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Effect of Cell Wall-Wounding Reagents on Agrobacterium-mediated Barley Seedling Transformation  

Choi, Jang-Won (Dept. of Bio-Industry, Daegu University)
Park, Hee-Sung (Dept. of Biotechnology, Catholic University of Daegu)
Publication Information
Journal of agriculture & life science / v.44, no.1, 2010 , pp. 9-15 More about this Journal
Abstract
Barley, a monocotyledonous plant, is relatively recalcitrant to the process of Agrobacterium-mediated genetic transformation. In this study, seedlings of six barley cultivars (Keunal-1-Ho, Saessal, Ol, Saechalssal, Seodunchal and Pungsanchalssal) were injured using alkali, oxidizing or reducing agents. They were then transformed using Agrobacterium via vacuum infiltration for the analysis of comparative GUS gene expression. It was determined that chemical injuries causing a slight growth retardation could overall enhance the GUS transformation rate. Hydrogen peroxide was determined to be the most effective.
Keywords
Agrobacterium; Barley seedling; Transformation; Wounding; GUS;
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