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Development of SCAR Marker for Identification of the Perilla Species  

Lee, Myoung-Hee (Department of Functional Crop, National Institute of Crop Science, RDA)
Yang, Ki-Woong (Department of Functional Crop, National Institute of Crop Science, RDA)
Ha, Tae Joung (Department of Functional Crop, National Institute of Crop Science, RDA)
Jung, Chan-Sik (Department of Functional Crop, National Institute of Crop Science, RDA)
Pae, Suk-Bok (Department of Functional Crop, National Institute of Crop Science, RDA)
Hwang, Chung-Dong (Department of Functional Crop, National Institute of Crop Science, RDA)
Park, Chang-Hwan (Department of Functional Crop, National Institute of Crop Science, RDA)
Baek, In-Youl (Department of Functional Crop, National Institute of Crop Science, RDA)
Kim, Hyeon-Kyeong (Pusan National University College of Medicine)
Park, Soon-Ki (Division of Plant Biosciences, Kyungpook National University)
Publication Information
Korean Journal of Breeding Science / v.43, no.4, 2011 , pp. 265-272 More about this Journal
Abstract
This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.
Keywords
Perilla frutescens; Molecular marker; Random Amplification of Polymorphic DNA (RAPD); Sequence Characterized Amplified Regions (SCARs);
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Hernandez P, Rosa R, Rallo L, Dorado G, Martin A. 2001. Development of SCAR markers in olive (Oleaeuropaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping. Theor. Appl. Genet. 103:788-791.   DOI   ScienceOn
2 Huh MK, Bang KH, Choi JS. 2007. Identification of Atractylodes japonica and A. macrocephala by AFLP and SCAR markers. Hort. Environ. Biotechnol. 48:201-205.
3 Ito M, Honda G. 1996. A Taxonomic Study of Japanese Wild Perilla (Labiatae). J. Phytogeogr. and Taxon. 44:43-52.
4 Ito M, Kato H, Oka Y, Honda G. 1998. Phytogenetic analysis of Japanese Perilla species by using DNA polymorphisms. Natural Medicines 52:248-252.
5 Jung CS, Lee MH, Oh KW, Kim HK, Park CB, Sung JD, Suh DY. 2005. Discovery of New Diploid Perilla Species in Korea. Korean J. of Breed. 37:152-154.
6 Kim CS, Lee GP, Han DH, Ryu KH, Lee CH. 2000. SCARs markers derived from RAPD for cultivar identification in Pyruspyrifolia. J. Kor. Soc. Hort. Sci. 41:125-128.
7 Nitta M, Ohnishi O. 1999. Genetic relationships among two Prerilla crops, shiso and egoma, and the weedy type revealed by RAPD markers. Genes Genet. Syst. 74:43-38.   DOI   ScienceOn
8 Paran I, Michelmore RW. 1993. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85:985-993.