Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Development of SCAR Marker for Identification of the Perilla Species

  • Lee, Myoung-Hee (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Yang, Ki-Woong (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Ha, Tae Joung (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Jung, Chan-Sik (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Pae, Suk-Bok (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Hwang, Chung-Dong (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Park, Chang-Hwan (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Baek, In-Youl (Department of Functional Crop, National Institute of Crop Science, RDA) ;
  • Kim, Hyeon-Kyeong (Pusan National University College of Medicine) ;
  • Park, Soon-Ki (Division of Plant Biosciences, Kyungpook National University)
  • Received : 2011.05.30
  • Published : 2011.09.30
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Abstract

This study is to generate SCARs markers for identification of Perilla species. A SCAR is a genomic DNA fragment at a single genetically defined locus that is identified by PCR amplification using a pair of specific oligonucleotide primers. We derived SCARs by sequencing and cloning the both ends of the amplified products of RAPD markers. Sixteen sequence-specific primers were synthesized from eight RAPD markers, which were completely sequenced. We developed the species-specific SCAR markers which could be used successfully in detecting genetic variation in four Perilla species. These markers could be used to verify species-origins of various forms of Perilla germplasms.

Keywords

References

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