• 제목/요약/키워드: ammonium sulfate

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Methylovorus sp. strain SS1 DSM 11726의 메탄올 대사와 세포외 다당류 생합성 (Metanol Metabolism and Extracellular Polysaccharide Biosynthesis in Methylovorus sp. strain SS1 DSM 11726)

  • 김재성;김시욱;김영민
    • 미생물학회지
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    • 제34권4호
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    • pp.207-211
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    • 1998
  • 메탄올을 이용하여 성장하는 Methylovorus sp. strain SS1은 formaldehyde의 산화를 위한 linear route의 주효소인 $NAD^+$-linked formaldehyde dehydrogenase 및 $NAD^+$-linked formate dehydrogenas와 cyclic route의 주효소인 hexulose-6-phosphate synthase, glucose-6-phosphate isomerae, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase 등의 활성을 나타내었는데, cyclic route에 관여하는 효소의 활성이 상대적으로 더 높았다. 이 세균은 formaldehyde의 동화와 관련된 ribulose monophosphate 경로의 주효소와 Entner-Doudoroff 경로의 주효소 및 transaldolase 활성과 함께 세포외 다당류 합성과 관련된 phosphoglucomutase, UDP-glucose pyrophyosphorylase, mannose-6-phosphate isomerase의 활성도 나타내었다. 2.3 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균은 7.6 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균보다 더 많은 세포외 다당류를 생산하였지만 균체 수율은 낮았고, 6-phosphogluconate dehydrogenase와 phosphoglucomutase 및 UDP-glucose pyrophoshorylase의 활성은 높게 나타내었으나 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase의 활성은 낮았다.

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Edwardsiella tarda에 대한 계란난황항체의 분리와 정제 (Isolation and purification of chicken egg yolk immunoglobulin against Edwardsiella tarda)

  • 김영대;오명주;정태성;정성주
    • 한국어병학회지
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    • 제17권1호
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    • pp.11-20
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    • 2004
  • Edwardsiella tarda로 면역한 닭의 난황항체 (IgY) 정제 방법을 비교하였다. Anti-E. tarda IgY의 정제는 PEG법, chloroform-PEG법, ammonium sulfate법과 정제 kit를 사용한 4가지 다른 방법으로 실시하였다. 정제된 IgY는 64 kDa의 heavy chain과 27 kDa의 light chain을 나타내었다. E. tarda로 면역된 IgY는 면역되지 않은 대조 IgY 보다 높은 ELISA가와 응집항체가를 나타내었으며, 정제된 IgY는 western blotting에서 anti-E. tarda 토끼혈청과 유사한 E.tarda 단백질을 인식하였다. PEG법과 ammonium sulfate법에 의해 정제된 IgY는 응집항체가가 1:512, chloroform-PEG법과 정제 kit에 의해 정제된 IgY는 1:128을 나타내었으며, PEG법이 IgY를 정제하기 위한 가장 빠른 방법이었다. 이 연구의 결과로 PEG법이 IgY의 생물학적 활성을 유지함과 더불어 신속하고 효과적인 정제방법임을 알 수 있었다.

고령토로부터 알루미나 추출에 관한 연구 (Study on the Alumina Extraction from Kaolin)

  • 맹중재;김철주;신병식
    • 한국세라믹학회지
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    • 제17권1호
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    • pp.35-41
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    • 1980
  • Alumina extraction from raw Hadong kaolin with sulfuric acid was studied to obtain relatively pure alumina. Factors as acid concentration, heating temperature and conditions of ammonium alum crystal formation from extracted solution are also surveyed and most of iron oxide in kaolin is eliminated in crystallization of ammonium alum. Pure crystal obtained from the extracted solution with ammonium sulfate is relatively free from iron containment in mother liquor. Alumina in ammonium alum crystal is converted to gibbsite form after complete hydrolysis in ammonia gas.

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Available Organic Carbon Controls Nitrification and Immobilization of Ammonium in an Acid Loam-Textured Soil

  • Choi, Woo-Jung;Lee, Sang-Mo;Han, Gwang-Hyun;Yoon, Kwang-Sik;Jung, Jae-Woon;Lim, Sang-Sun;Kwak, Jin-Hyeob
    • Journal of Applied Biological Chemistry
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    • 제49권1호
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    • pp.28-32
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    • 2006
  • Effect of organic-C on immobilization and nitrification patterns in acidic soil was examined during 20 weeks incubation period to verify if organic amendments such as composted material can increase soil retention of N by stimulating microbial immobilization of $NH_4^+$. Four treatments were laid out: control without fertilizer N and glucose (treatment code: S), ammonium sulfate (SN), ammonium sulfate with single glucose at the commencement (0 week) of incubation (SNG), and ammonium sulfate with double glucose at 0 and 4 weeks of incubation (SNGG). Glucose application (SNG) significantly increased microbial immobilization of $NH_4^+$ within 1 week of incubation over SN. Immobilization was followed by remineralization thereafter; however, second-application of glucose (SNGG) restored $NH_4^+$ immobilization. At the same time, nitrification was significantly inhibited by glucose application as indicated by consistently low $NO_3^-$ concentration in SNG and SNGG soils, suggesting that microbial assimilation of $NH_4^+$ is predominant compared to nitrification when available C-source is abundant. These results suggest application of chemical fertilizer-N with organic amendment would have beneficial effect on soil-N retention and environmental conservation by reducing production of $NO_3^-$ which is likely to be lost through leaching or denitrification.

봄철과 가을철 용인지역 입자상 물질의 이온 농도 (Ion Concentrations of Particulate Matter in Yongin in Spring and Fall)

  • 원수란;최용주;김아롱;최순호;김영성
    • 한국대기환경학회지
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    • 제26권3호
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    • pp.265-275
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    • 2010
  • Mass and ion concentrations of $PM_{10}$ and $PM_{2.5}$ were measured in Yongin, downwind of the Seoul metropolitan area. Twenty-four hour average samples were collected daily for a month or so in spring and fall of both 2007 and 2008. Asian dust occurred twice in each period except in the fall of 2007. During the Asian dust period, nitrate in fine particles decreased since large amount of cations in coarse particles of Asian dust absorbed it. In spring 2008, sulfate as well as ammonium also increased in coarse particles. In spite of occasional Asian dust events, the influence of secondary formation was dominant over the period. Excessive ammonium over sulfate was enough to neutralize nitrate as well. High correlation coefficients between $K^+$ and sulfate, nitrate, and ammonium indicate a close relationship between secondary formation and biomass burning. Biomass burning and open burning are considered to be important sources of chloride.

두부폐액(廢液)의 조성(組成) 및 Saccharomyces Cerevisiae를 이용(利用)한 Alcohol 발효(醱酵) (Soybean Whey Composition and Alcohol Fermentation by Using Saccharomyces Cerevisiae)

  • 최미애;최경호
    • 한국식품영양과학회지
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    • 제11권2호
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    • pp.31-35
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    • 1982
  • 폐액으로 방류되는 두부 폐수(whey)를 이용하여 alcohol 발효를 실시하였으며 다음과 같은 결과를 얻었다. whey중(中)에 함유(含有)된 환원당은 약 0.71%로서 이당(糖)을 이용(利用)하며 l당(當) 최대(最大) 1.86 g의 ethanol이 생산(生産)되었으나 수률(收率)이 낮아서 경제성은 희박하다. 그러나 발효에 의해서 단백질과 당이 각각 94%, 81%만큼이나 감소한 결과(結果)로 미루어 보아 두부 폐수중(廢水中)의 유기물(有機物)을 alcohol 발효(醱酵)에 이용(利用)할 수 있음을 확인(確認)하였다.

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1996/1997년 겨울 우리나라 배경측정소에서의 대기오염 물질 특성 (Characteristics of Air Pollutants at Three Background Sites in Korea in the Winter of 1996/1997)

  • 김용표
    • 한국대기환경학회지
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    • 제16권4호
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    • pp.415-420
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    • 2000
  • The daily mean concentrations of PM2.5 mass ionic species carbonaceous species and HNO3, HCl, and NH3 were analyzed at three background sites in Korea : Kangwha, Kosan, and Dongbaik during two intensive field studies between December 1996 and January 1997, Four-day backward trajectory analysis was also carried out. The mean concentrations of anthropogenic species were higher at Kangwha and Dongbaik than Kosan. At these two sites themean concentrations of nitrate and ammonium/ammonia were higher than those of Kosan. It was suggested that these two sites be considered as the background sites of the Seoul Metropolitan Area and Pusan respectively. Major chemical components in fine particles were sulfate organic carbon nitrate and ammonium for all the sites. More than 90% of sulfate were non-sea-salt(nss) sulfates and most of the nss sulfate in PM2.5 might be present as ammonium sulfates at all sites. Most of air parcels arriving a Kangwha and Dongbaik were from the northern China. At Kosan during the measurement period half of air parcels were from the northern China and the other half from the southern China. At Kosan the concentrations of anthropogenic air pollutants originated from the southern China were higher than those typically observed from the northern China.

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Measurement of Free Polysaccharide in Tetanus Toxoid-Conjugate Vaccine Using Antibody/Ammonium Sulfate Precipitation

  • Yoo, Tae-Hyeon;Kim, Hyun-Sung;Park, Sung-Sik;Bang, Eun-Young;Oh, Yong-K.;Kim, Li-Seop;Kim, Hun;Hur, Byung-Ki;Ryu, Yeon-Woo;Kim, Jong-Su
    • Journal of Microbiology and Biotechnology
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    • 제13권3호
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    • pp.469-472
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    • 2003
  • A method that effectively precipitates capsular polysaccharide of Haemophilus influenzae type b (polyribosylribitol phosphate, PRP) conjugated to tetanus toxoid (TT), PRP TT in a liquid vaccine has been developed to measure free PRP present in TT-conjugate vaccine. The method involves adding anti-TT antibody and ammonium sulfate to precipitate PRP-TT conjugate and measuring free PRP in tile supernatant. This new method provides a complete precipitation of the total PRP-TT, and provides an accurate and reproducible measurements of free PRP. The accuracy of the assay was confirmed by spiking known amounts of unconjugated PRP to PRP-TT conjugate, and the new method was found to have no effect on free PRP while precipitating PRP-TT. The published acid precipitation method did not produce reproducible results due to incomplete precipitation of PRP-TT, especially when the vaccine is formulated in a salt-buffered solution.

수산을 사용한 크롬도금의 광택성에 미치는 전류밀도와 전류효율의 영향 (Effect of Current Density and Current Efficiency on the Decorative Property of Chromium Deposits using Oxalic Acid)

  • 오이식;박정덕
    • 동력기계공학회지
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    • 제5권1호
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    • pp.89-96
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    • 2001
  • Decorative property of chromium deposition from oxalic acid bath containing chromium oxide and ammonium sulfate, has been examined over a wide range of bath compositions and plating conditions. The followings were determined as optimum bath composition, $CrO_3\;200{\sim}250g/{\ell},\;H_2C_2O_4{\cdot}2H_2O\;500{\sim}700g/{\ell},\;(NH_4){_2}SO_4\;40{\sim}120g/{\ell}$, and operation conditions; pH $2.0{\sim}2.5$, current density of $15{\sim}250Adm^2 $ at the bath temperatures of $30{\sim}80^{\circ}C$. Bright chromium deposits were obtained over a wide range of ammonium sulfate concentration, bath temperature, and current density. The current efficiency decreased with increasing current density and bath pH, and increased with Increasing bath temperature. The highest current efficiency was obtained in the bath containing $80g/{\ell}$ of ammonium sulfate. Bright chromium deposits were not obtained at conditions of all the highest current efficiencies.

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딸기복비(複肥), 염화가리(鹽化加里), 황산가리(黃酸加里) 및 질산(窒酸)암모늄이 딸기의 수량(收量)과 품질(品質)에 미친 영향(影響) (The Effect of Strawberry Compound Fertilizer, Potassium Chloride, Potassium Sulphate and Ammonium Nitrate on the Yield and Quality of Strawberry)

  • 오왕근
    • 한국토양비료학회지
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    • 제19권1호
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    • pp.9-13
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    • 1986
  • 황산칼리와 인산암모니아를 주축으로 한 경기화학공업주식회사인 시제 딸기복비의 비효를 염화칼리, 황산칼리, 질산암모니아에 대비하여 비닐하우스에서 딸기, 보교조생 (Fragaria Grand EHRD)으로 시험한 결과를 요약하였다. 가수분해당을 포함하는 전당의 함량은 높였다. 일반적으로 염화칼리보다 황산칼리가 딸기즙의 딸기의 생육, 화방수.,수량에 미친 시제 딸기복비의 효과는 염화칼리, 황산칼리, 질산암모니아와 통계적인 차이가 없으나 딸기즙의 환원당, 묽은 산당분함량을 높였으며 딸기복비가 딸기즙의 당 함량을 높인것도 황산칼리 때문인 것으로 생각되었다.

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