• Title/Summary/Keyword: alpha-cellulose

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Cellulose Acetate Membrane Electrophoresis of Bull Serum Protein (Cellulose Acetate막(膜) 전기영동법(電氣泳動法)에 의한 종모우(種牡牛)의 혈청단백분획(血淸蛋白分劃)에 관한 조사(調査))

  • Lee, Chang-Woo
    • Korean Journal of Veterinary Research
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    • v.22 no.2
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    • pp.115-119
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    • 1982
  • Total protein levels of 60 bovine bull sera were treasured with refractometer and the strum proteins were fractionated by cellulose acetate membrane electorphoresis and the relative amount of each fraction was measured with automatic scanning densitometer. The sixty bovine bulls consisted of 17 Charolais bulls, 28 Holstein bulls and 15 Korean bulls. Mean total serum protein level of the total bulls was $7.50{\pm}0.50(Mean{\pm}SD)$, with the mean of Charolais $7.04{\pm}0.37g/dl$, the mean of Holstein $7.62{\pm}0.40g/dl$ and the mean of Korean $7.81{\pm}0.43g/dl$. As barbital-calcium buffer was used in fractionating the bovine serum protein with cellulose acetate membrane, electric current of 0.4 mA per centimeter width of the membrane for an hour resulted in more clear separation between ${\alpha}_1$-globulin and ${\alpha}_2$-globulin than electric current of 0.6 or 1.0 mA for an hour. However ${\alpha}_1$-globulin and ${\alpha}_2$-globulin could not be measured separately with automatic scanning densitometer. Mean serum albumin level of total bulls was $3.54{\pm}0.42g/dl$, with the mean of Charolais $3.42{\pm}0.26g/dl$, the mean of Holstein $3.69{\pm}0.38g/dl$ and the mean of Korean $3.39{\pm}0.55g/dl$. Mean serum ${\alpha}$-globulin level of the total bulls was $0.62{\pm}0.14g/dl$, with the mean of Charolais $0.61{\pm}0.07g/dl$, the mean of Holstein $0.60{\pm}0.11g/dl$ and the mean of Korean $0.67{\pm}0.23g/dl$. Mean ${\beta}$-globulin level of the total bulls was $0.85{\pm}0.21g/dl$, with the mean of Charolais $0.74{\pm}0.11g/dl$, the mean of Holstein $0.83{\pm}0.14g/dl$ and the mean of Korean $1.02{\pm}0.30g/dl$. Mean serum ${\gamma}$-globulin level of the total bulls was $2.48{\pm}0.45g/dl$ with the mean of Charolais $2.27{\pm}0.37g/dl$, the mean of Holstein $2.48{\pm}0.44g/dl$ and the mean of Korean $2.72{\pm}0.47g/dl$.

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The Chemical Composition of Abies koreana Wilson Wood (구상나무(Abies koreana Wilson)재(材)의 화학적(化学的) 조성(組成))

  • Moon, Chang Kuck;Pack, Chong Yawl;Kang, Wee Pyung
    • Journal of Korean Society of Forest Science
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    • v.50 no.1
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    • pp.1-4
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    • 1980
  • The Chemical components of Abies koreana Wilson grown in Korea were analized. The results obtained were summarized as follows. 1. The ash content is ca 0.56% on the average, and of the range is 0.33 to 0.76%. 2. The cold water extractive content is ca 8.76% on the average and of the range is 5.55 to 12.5%. 3. The hot water extractive content is ca 10.16% on the average and of the range is 4.80 to 13.65%. 4. Basic extractive content is ca 14.60% on the average and of the range is 5.51 to 25.44%. 5. The alcohol benzol soluble fraction is ca 4. 23% and of the range is 2.94 to 5.44%. 6. The holocellulose content is ca 76.49% on the average and of the range is 73.68% to 79.10%. 7. The cellulose content is ca 56.30% on the average and of the range is 46.02% to 61. 33%. The cellulose contains 78.54% ${\alpha}$-cellulose, 7.66% ${\beta}$-cellulose and 14.04% ${\gamma}$-cellulose respectively. 8. The Klason lignin content is ca 25.03% on the average and of the range is 22.5 to 27.0%. In conclusion, ash content is comparable to the other needle leaf trees. It has 76.49% in holocellulose content which is comparable value to the Pinus densiflora's. It has lower value than the Pinus densiffora in pentosan and lignin content. Having not resin cannal in xylem and long tracheid, this wood could be usable industrial material.

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Characterization of Crude $\alpha-fucosidase$ from Bacteroides fragilis Roid8 (Bacteroides fragilis Roid8의 $\alpha-fucosidase$ 조효소 특성)

  • Heo Soon Young;Park Myung Soo;Kwon Bin;Ji Geun Eog
    • KSBB Journal
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    • v.20 no.5 s.94
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    • pp.355-358
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    • 2005
  • Bacteroides is the most predominant bacteria in the human large intestinal tract. Bacteroides fragilis RoidB isolated from a Korean faecal sample showed high $\alpha-fucosidase$ activity compared to other bacterial species. The optimized meidum for the production of $\alpha-fucosidase$ from 8. fragilis contained BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K1mg, and starch 5g per 1 L. The crude $\alpha-fucosidase$ obtained through DEAE-sepharose and CM- cellulose chromatography showed optimum temperature and pH at $40^{\circ}C$ and 7.0, respectively. Among several metals, $Co^{++}\;and\;Zn^{++}$ showed strong inhibition on enzyme activity.

Production of Amylases from Herpetosiphon geysericola (Herpetosiphon geysericola 균주의 Amylase 생성)

  • Jun, Yeong-Soo;Seu, Jung-Hwn
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.2
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    • pp.188-191
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    • 1985
  • A thermophilic and cellulolytic bacterium, Herpetosiphon geysericola CUM 317 isolated from the compost, produced ${\alpha}-amylase,\;{\beta}-amylase$, and glucoamylase. Mutual relationships on the production of the three amylases were studied by changing the cultivation conditions. ${\alpha}-Amylase$ and glucoamylase were produced highly after 40 hrs on wheat bran medium at $50^{\circ}C$ and after 30 hrs on liquid medium at $40^{\circ}C$, though ${\beta}-amylase$ was produced best at 10 hrs of initial cultivation phase. The production of the amylases was generally repressed by the addition of carbon sources in liquid medium containing polypeptone. ${\alpha}-Amylase$ production was enhanced relatively by the addition of cupric sulfate in the liquid medium, ${\beta}-amylase$ was enhanced by cadmium sulfate, and glucoamylase was enhanced by calcium chloride.

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The Comparison of Absorption Characteristics between High Absorbent Polymers and Cellulose (고흡수성(高吸水性) Polymer와 Cellulose의 흡수특성(吸水特性) 비교(比較))

  • Yang, In;Ahn, Won-Yung
    • Journal of the Korean Wood Science and Technology
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    • v.18 no.2
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    • pp.36-48
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    • 1990
  • This experiment was carried out to improve the quality of high absorbent polymer which has excellent absorptivity and water retention compared to pulp and absorption sheet, through absorption characteristics-absorptive power, water retention, absorption rate, gel strength, pH, particle size, and moisture content. - of six polymers, namely, anionic polyacrylamide, cationic polyacrylamide, polyacrylic, acid polyvinyle alcohol 500, and 1500, and a-cellulose. and to examine the possibility of substitution of amide groups for carboxyl group and/or hydroxyl group which were commercial high absorbent polymer by comparing the absorption characteristics of the polymers. Polyacrylamide has high absorptive power and water retention, but has low gel strength and poor absorption rate. The rest of polymers were similiar to ${\alpha}$-cellulose in every respect. Thus, polyacrylamides could be replaced with polyacrylic acid and polyvinyl alcohol which are presently a high absorbent polymers. In comparing the absorption characteristics and the absorptive power of the polymers-anionic polyacrylamide, cationic polyacrylamide, polyacrylic acid, polyvinyle alcohol. a-cellulose-the absorptive power was in inverse proportion to the gel strength and absorption rates, affected by the particle size and pH change.

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Kinetics of Oil-Proof Agent Adsorption onto Cellulose Fibers

  • Zhu, Hongxiang;Honghu, Zeng;Wang, Shuangfei;Lee, Hak-Lae
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.41 no.5
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    • pp.59-65
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    • 2009
  • Adsorption of an oil-proof agent (OPA)onto cellulose fibers during the papermaking process was investigated using UV-Vis spectrophotometry by measuring the OPA concentration decrease in the solution. From the calibration curve, the spectrum were converted to chemical concentrations in solution, from which the amount adsorbed onto the fiber surface could be determined. Thus, it was possible to determine the total amount adsorbed onto the fibers and in solution. Using this approach, we studied the adsorption behavior of the OPA onto the fiber surface and derived its, ${\Gamma}^s_t={\Gamma}^s_{\infty}(1-e^{-k_{\alpha}t})$. The values of the parameters kaand ${\Gamma}^s_{\infty}$ were determined using a mathematic model based on a mass transfer equation. Ultimately, a complete was derived: $Q={\alpha}{\cdot}\sum\limits_{i-1}^m{\pi}d_il_i{\cdot}M_A{\cdot}(1-e^{-k_{\alpha}t})/A_N$.

Detection of Alpha Tracks of Boron by Nuclear Reaction with Neutron (중성자 핵반응에 의한 보론의 알파트랙 검출)

  • Sohn, Se Chul;Pyo, Hyung Yeal;Park, Yong Jun;Jee, Kwang Yong;Kim, Won Ho
    • Analytical Science and Technology
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    • v.17 no.1
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    • pp.8-15
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    • 2004
  • The detection efficiencies of the several solid track detectors were investigated for the determination of boron content in aqueous solution by using the alpha muti-Radioisotope(RI) source. Polycarbonate (Lexan and CR-39) and cellulose nitrate (CN-85 and LR-115) were selected as materials for alpha track detection of boron. Alpha muti-RI source, uranium metal particles and boron standard solution were used for alpha emission. In this study, four solid track detectors(CN-85, LR-115, Lexan and CR-39) were characterized under various etching conditions as well as neutron irradiation conditions. As a result, the CN-85 was turned out to be best to provide good efficiency among the four detectors. The selected solid track detector was utilized for the determination of trace amount of boron in aqueous sample and its results were discussed in the text.

Retarding Effect of Dietary Fiber Isolated from Persimmon Peels and Juubes on in vitro Glucose, Bile Acid, and Cadmium Transport (감과피와 대추로부터 분리한 식이섬유의 포도당, 담즙산 , 카드뮴 투과 억제에 관한 in vitro 연구)

  • 이혜진
    • Journal of Nutrition and Health
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    • v.31 no.4
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    • pp.809-822
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    • 1998
  • Retarding effects of the dietary fibers from persimmon peels (PTDF ; total dietary fibers, PIDF ; insoluble dietary fibers, PSDF ; soluble dietary fibers) and dried jujubes (JTDF ; total dietary fibers, JIDF ; insoluble dietary fibers, JSDF ; soluble dietary fibers) on glucose, bile acid and cadmium transport were evaluated by in vitro dialysis sack method. These effects were compared with those of commerical citrus pectin, CM-cellulose (CMC) and $\alpha$-cellulose. Yields of PTDF, PIDF and PSDF on wet weight basis were 14.04% , 11.61%, 1.76%, respectively, and those from dried jujubes were 14.80%, 9.98% and 2.80%, respectively. The amount of soluble fibers in JTDF was higher than PTDF. Soluble fibers had the retarding effects on glucose transport but insoluble fibers did not have. CM-cellulose showed the greatest retarding effect, which was followed by citrus pectin and JSDF. Among the soluble fibers, PSDF had the lowest retardig effect. Retarding effect of TDF was dependent upon the amount of SDF in TDF. Regarding bile acid dialysis , insoluble dietary fibers as well as soluble dietary fibers showed the retarding effects, among which JSDF had the greatest retarding effects, among which JSDF had the greatest retarding effect, followed by citrus pectin. Among the extracted fibers, dietary fibers from dried jujubes were more effective than these from persimmon peels, and SDF seemed to show higher retarding effects than IDF and TDF. On cadmium transport retardation , all dietary fibers except $\alpha$-cellulose had the retarding effects and PSDF showed the greatest effect which was followed by PIDF and CMC, The extracted fibers showed higher retarding effect on Cd transport than glucose and bile acid transport, and dietary fibers from persimmon peels showed higher retarding effects than those from dried jujubes.

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Effect of Biological and Liquid Hot Water Pretreatments on Ethanol Yield from Mengkuang (Pandanus artocarpus Griff)

  • Yanti, Hikma;Syafii, Wasrin;Wistara, Nyoman J;Febrianto, Fauzi;Kim, Nam Hun
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.2
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    • pp.145-162
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    • 2019
  • This study aimed to increase the sugar and ethanol yield from the mengkuang plant biomass through biological and liquid hot water (LHW) pretreatment and their combination. The results showed that biological pretreatments with 5% inoculum of the fungus Trametes versicolor resulted in the highest alpha cellulose content incubated for 30 days, and 10% inoculum resulted in the lowest lignin content. LHW pretreatment decreased the hemicellulose content of pulps from 10.17% to 9.99%. T. versicolor altered the structure of the mengkuang pulp by degrading the lignin and lignocellulose matrix. The resulting delignification and cellulose degradation facilitate the hydrolysis of cellulose into sugars. The alpha cellulose content after biological-LHW pretreatment was higher (78.99%) compared to LHW-biological pretreatment (76.85%). Scanning electron microscopy analysis showed that biological-LHW combinated treatment degrades the cell wall structures. The ethanol yield for biological-LHW pretreated sample was observed 43.86% (13.11 g/L ethanol by weight of the substrate, which is much higher than that of LHW-biological pretreatment (34.02%; 9.097 g/L). The highest reducing sugar content about 45.10% was observed with a resulting ethanol content of 15.5 g/L at LHW pretreatment temperature of $180^{\circ}C$ for 30 min.

Hydrothermal Pretreatment of Ulva pertusa Kjellman Using Microwave Irradiation for Enhanced Enzymatic Hydrolysis (구멍갈파래의 효소 가수분해 증진을 위한 마이크로파 이용 열수 전처리)

  • Kim, Jungmin;Ha, Sung Ho
    • Korean Chemical Engineering Research
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    • v.53 no.5
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    • pp.570-575
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    • 2015
  • The green algae have cellulose as a main structural component of their cell wall and the cellulose content in green algae is much higher than other marine algae such as brown algae and red algae. Furthermore, green algae do not contain lignin in their cell wall and store starch as food in their plastids. Thus, it was investigated that the effect of hydrothermal pretreatment process utilizing microwave irradiation for Ulva pertusa Kjellman, a division of green algae, which is expected to be utilized for bioenergy production, on the enzymatic hydrolysis. The hydrothermal temperature have an effect on the pretreatment of Ulva pertusa Kjellman, but the effect of power of microwave irradiation is negligible. The rate of enzymatic hydrolysis was increased as the hydrothermal temperature increased until $140^{\circ}C$. The enzymatic hydrolysis of pretreated Ulva pertusa Kjellman under the optimum pretreatment conditions (50 W of microwave irradiation power and $150^{\circ}C$ of hydrothermal temperature) with cellulase, ${\alpha}$-amylase, and Novozyme 188 having ${\beta}$-glucosidase acitivity resulted in the saccharification of 96 wt% of total carbohydrate in Ulva pertusa Kjellman during 3 hrs, while it took 24 hrs for the enzymatic hydrolysis of untreated Ulva pertusa Kjellman. It confirmed that the hydrothermal pretreatment was effective on Ulva pertusa Kjellman for the enzymatic hydrolysis.