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Characterization of Crude $\alpha-fucosidase$ from Bacteroides fragilis Roid8  

Heo Soon Young (Department of Food and Nutrition, Seoul National University)
Park Myung Soo (Research Center, BIFIDO Co. Ltd)
Kwon Bin (Department of Food and Nutrition, Seoul National University, Research Center, BIFIDO Co. Ltd)
Ji Geun Eog (Research Institute of Human Ecology, Seoul National University)
Publication Information
KSBB Journal / v.20, no.5, 2005 , pp. 355-358 More about this Journal
Abstract
Bacteroides is the most predominant bacteria in the human large intestinal tract. Bacteroides fragilis RoidB isolated from a Korean faecal sample showed high $\alpha-fucosidase$ activity compared to other bacterial species. The optimized meidum for the production of $\alpha-fucosidase$ from 8. fragilis contained BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K1mg, and starch 5g per 1 L. The crude $\alpha-fucosidase$ obtained through DEAE-sepharose and CM- cellulose chromatography showed optimum temperature and pH at $40^{\circ}C$ and 7.0, respectively. Among several metals, $Co^{++}\;and\;Zn^{++}$ showed strong inhibition on enzyme activity.
Keywords
Bacteroides fragilis; characterization;
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