• Journal of Acupuncture Research
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• 제18권2호
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• pp.136-149
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• 2001

Objetives : This study was done to evaluate the antiallegic effect of medicinal acupuncture solution of Acanthopanax senticosus(Rupr. et Maxim;abbreviated as ASMA), studies were done experimentally. Methods : For this aim MTT assay, anaphylaxis reaction, DTH, histamine release, IgE production and vascular permability was evaluated. Results : ASMA didn't show an effective cytotoxicity on the mouse lung fibroblast. It insignifcantly suppressed histamine release from mast cells induced by compound 48/80, while it significantly reduced IgE production and SRBC-challenged DTH(Delayed type hypersensitivity). ASMA effectively suppressed anaphylactic reaction induced by compound 48/80. However, it didn't inhibit vascular permeability and contact dermatitis significantly. Conclusion:Medicinal acupucture solution had antialletgic effects. Acanthopanax senticosus medical acupuncture depress the allergy reaction. Especially in anaphylactic type, the effect was good.

• 대한한방내과학회지
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• 제29권1호
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• pp.12-24
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• 2008

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.

• 대한한방내과학회지
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• 제28권3호
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• pp.453-463
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• 2007

Backgrounds : Glutathione-s-transferase (GST) is a kind of phase II metabolism enzyme and plays an important role in the detoxification of various toxic chemicals. It was reported that the genetic polymorphism of GSTM1 and GSTT1 genes may be responsible for asthma development and susceptibility to allergy. Traditional oriental medicine uses a unique diagnostic technique. differentiation-syndrome. to analyze signs and symptoms of patients synthetically. Through differentiation-syndrome. asthma patients can be divided into two groups: the deficiency syndrome group (DSG) and the excess syndrome group (ESG). Objectives : The purpose of this study was to investigate the possible association of GST gene polymorphism with clinical phenotype by differentiation-syndrome of bronchial asthma patients. Materials and Methods : One hundred and ten participants were evaluated by pulmonary function test. Patients with 53 DSG and 31 ESG by differentiation-syndrome were assessed for genetic analysis. GSTM1 and GSTT1 deletion polymorphism was performed by polymerase chain reaction (PCR). Results : GSTM1 gene deletion was detected in 43.4% of individuals in the DSG and in 38.71 % in the ESG. The distribution of GSTM1 polymorphism between DSG and ESG was not significantly different [$x^2$=0.1767, p=0.6742; OR(95% CI)=1.2139(0.4915-2.9979)]. The proportion of GSTT1 null genotypes was 41.51% in the DGS and 45.16% in the ESG. The distribution of GSTT1 polymorphism between DSG and ESG was also not significantly different [$x^2$=0.1065, p=0.7442; OR(95% CI)=0.8618(0.3525-2.1065)]. In the combined analysis of GSTM1 and GSTT1 genes, the frequency of both null type of GSTM1/GSTT1 genes was not significantly different from both positive type of GSTM1/GSTT1 genes[$x^2$=0.0768, p=0.7817; OR(95% CI)=1.2000(0.3303-4.3602)] Conclusions : These results indicate that polymorphism of the GST gene might not be associated with the symptomatic classification of DSG and ESG by differentiation-syndrome in Korean asthmatics.

• IMMUNE NETWORK
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• 제22권2호
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• pp.17.1-17.14
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• 2022

We aimed to investigate associations of dietary diversity (DD) with gut microbial diversity and the development of hen's egg allergy (HEA) in infants. We enrolled 68 infants in a high-risk group and 32 infants in a control group based on a family history of allergic diseases. All infants were followed from birth until 12 months of age. We collected infant feeding data, and DD was defined using 3 measures: the World Health Organization definition of minimum DD, food group diversity, and food allergen diversity. Gut microbiome profiles and expression of cytokines were evaluated by bacterial 16S rRNA sequencing and real-time reverse transcriptase-polymerase chain reaction. High DD scores at 3 and 4 months were associated with a lower risk of developing HEA in the high-risk group, but not in the control group. In the high-risk group, high DD scores at 3, 4, and 5 months of age were associated with an increase in Chao1 index at 6 months. We found that the gene expression of IL-4, IL-5, IL-6, and IL-8 were higher among infants who had lower DD scores compared to those who had higher DD scores in high-risk infants. Additionally, high-risk infants with a higher FAD score at 5 months of age showed a reduced gene expression of IL-13. Increasing DD within 6 months of life may increase gut microbial diversity, and thus reduce the development of HEA in infants with a family history of allergic diseases.

• Korean Journal of Acupuncture
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• 제20권3호
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• pp.81-90
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• 2003

Objectives : This experimental research has been done to study the effects of herbal-acupuncture with Juglandis Semen on the anti-allergic reaction. Methods : ICR mice were injected intraperioneally with compound 48/80 to induce the immediately type hypersensitivity (anaphylaxis). ICR mice were sensitized by PC and SRBC to induce the delayed type hypersensitivity. Sprage-Dawley rats were injected subcutaneously with egg albumin to measure the WBC. ICR mice and Sprage-Dawley rats were injected with Juglandis Semen herbal-acupuncture for the anti-allergic reaction test. Results : 1. In the restraint effect of systemic anaphylactic reaction, the mortality of mice decreased 20% each before 10 minutes and after 5 minutes. 2. In the contact dermatitis induced by PC, both Treat Ⅰand Treat Ⅱ group showed significant(p<0.01, p<0.05) decrease in comparison with the Control group, and also Treat Ⅲ group showed significance(p<0.005). 3. In the delayed type hypersensitivity in mice challenged by SRBC, Treat Ⅰ group showed significant(p<0.05) decrease in comparison with the Control group, and there was significance(p<0.05, p<0.05) in Treat Ⅲ and Treat Ⅳ group. 4. The change of the WBC in allergic rats induced by egg albumin showed significant(p<0.01, p<0.05, p<0.05) decrease in Treat Ⅰ, Treat Ⅱ and Treat Ⅲ group. Conclusions : The restraint effects of Juglandis Semen herbal-acupuncture on the anti-allergic reaction showed significance statistically. And also allergic group treated with $BL_{17}$ acupuncture (Treat Ⅲ group) showed same results.

• Journal of Ginseng Research
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• 제33권2호
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• pp.93-98
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• 2009

To understand the anti-allergic mechanism of compound K, which is a metabolite of ginsenoside Rb1, a main constituent of the root of Panax ginseng C.A. Meyer (family Araliaceae), its inhibitory effect against IgE-antigen complex IAC)-induced passive cutaneous anaphylaxis (PCA) reaction in mice and mRNA and protein expressions of allergic cytokines in lAC-stimulated RBL-2H3 cells were investigated. Orally administered ginsenoside Rb1 more potently inhibited PCA reaction when administered at 5 h prior to the lAC treatment than when administered at I h before. However, compound K orally administered 1 h before lAC treatment showed a more potent anti-PCA reaction effect than when treated at 5 h before. Orally administered ginsenoside Rb1 more potently inhibited PCA reaction induced by lAC in mice than intraperitoneally treated one, apart from orally administered its metabolite, compound K, which was more potent than the orally administered one. The compound K, a metabolite of ginsenoside Rb1, inhibited mRNA and protein expressions of IL-4 and TNF-${\alpha}$ and the activation of their transcription factor NF-$\kappa$B and MAPK in lAC-stimulated RBL-2H3 cells. These findings suggest that orally administered ginsenoside Rb1 may be dependent on its metabolism by intestinal microflora in the intestine and the compound K may improve allergic diseases by the inhibition of IL-4 and TNF-${\alpha}$ expresseion.

• 동의생리병리학회지
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• 제18권3호
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• pp.857-862
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• 2004

The purpose of this research was to investigate the effects of supercritical fluid extract of Kamichungbieum (SFE) on allergic reaction. SFE (500 mg/kg) inhibited the systemic anaphylaxis induced by compound 48/80 or platelet activating factor and inhibited the passive cutaneous anaphylaxis (PCA) induced by anti-dinitrophenyl (DNP)-lgE and DNP-human serum albumin (HSA) in vivo. Also, SFE inhibited the SRSC-induced delayed type hypersensitivity and inhibited the hind paw edema induced by histamine. In addition, SFE inhibited the permeability of evans blue induced by acetic acid and inhibited the writhing syndrome induced by acetic acid. These results indicate that SFE may be useful for the prevention and treatment of allergy related disease.

• 대한약침학회지
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• 제2권1호
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• pp.1-11
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• 1999

We have studied Bee Venom Therapy to treat Sequalae of Sprain, and obtained following results. 1. The treatment of sprain is using the effect of the affected part on Bee Venom Therapy. 2. For the treatment of sprain, it is often used the near acupuncture point of injury(近位取穴) or painful point to press(阿是穴). 3. Bee Venom Therapy is one of the powerful treatment, so we are using the sequalae of sprain in the case of not cured by Acupuncture, Cupping or another therapy. 4. Bee Venom injection is started a small quantity, and is increased slowly by degrees. 5. The immune response by Bee Venom Therapy is a sort of Allergy, and it is classified large delayed reaction. It is considered positive reaction. 6. We have selected Acupuncture Points from one to four by the degree of injury. 7. The period of treatment is some different from the degree of injury, but generally we have obtained good results to treat from 5 to 20 by Bee Venom Therapy.

• 동의생리병리학회지
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• 제17권6호
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• pp.1487-1492
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• 2003

Gunjung-tang(GJT) has been reported at Shanghanlun(傷寒論), which has been used for the treatment of general weakness, digestive organ disease and the treatment of allergic disorders in clinical medicine. However, its effect in experimental models remains unknown. In a rat and mouse model, the role of GJT was examined in mast cell-dependent allergic reactions and secretion of inflammatory cytokines. GJT inhibited anti-dinitrophenyl IgE antibody-induced passive cutaneous anaphylaxis reaction by Intraperitoneal pretreatment. GJT inhibited both IL-1β and TNF-α secretion on egg albumin induced allergic tissues and compound 48/80 induced thymus. Furthermore, GJT enhanced Band T lymphocytes proliferation. Our findings provide evidence that GJT inhibits the IgE-dependent allergic reactions and inflammatory cytokines secretion, and enhanced immune response.

• Clinical and Experimental Pediatrics
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• 제54권7호
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• pp.304-309
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• 2011

Purpose: There are very few reports of adverse drug reactions (ADR) and almost no study of drug provocation test (DPT) in Korean children. We aimed to assess the role of DPT in children with unpredictable ADRs, and compare the causative drugs and clinical characteristics between detailed history of ADRs and result of DPTs. Methods: We included 16 children who were experienced ADRs referred to pediatric allergy clinic at Ajou University Hospital (January 2006 to December 2009). With various suspected drugs, 71 DPTs were done in 16 patients using our own protocol, and skin tests to antibiotics were combined in ADRs to antibiotics in medical history. Results: There were 17 (23.9%) positive DPTs results out of 71 individual DPTs, and 11 patients (68.8%) from 16 patients were positive to at least one drug. Drugs causing positive reactions were acetaminophen in 5 (31%), Non-steroidal anti-inflammatory drugs in 4 (25%), penicillin in 3 (19%), cephalosporin in 2 (13%), and cotrimoxazole, macrolide and lactose in 1 each. Conclusion: DPT seems a safe and useful procedure to confirm causative drug and identify safely administering alternative drugs in children with ADR.