• Title/Summary/Keyword: alcohol extraction

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Volatile Flavor Components of Korean Auricularia polytricha (Mont.) sacc. Mushroom (한국산 털목이버섯의 휘발성 향기성분)

  • Lee, Jae-Gon;Do, Jae-Ho;Sung, Hyun-Soon;Lee, Jong-Won
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.546-548
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    • 1995
  • An attempt was made to determine the volatile flavor components of Auricularia polytricha mushroom. Essential oils from the dried mushroom were isolated by a simultaneous steam distillation-extraction(SDE) method using diethyl ether as a solvent. Total 30 components were identified bt GC-MS from the in essential oils including 11 acids, 10 alcohols, 5 aldehydes, 3 ketons, 1 other components. The major volatile components were heaxadecanoic acid(16.74%), benzeneethanol(7.77%), pentadecanoic acid(7.59%), dihydro-5-penhtyl-2-(3H)-furanone acid(4.28%), tetradecanoic acid(3.37%), pentanoic acid(3.38%) and 1-octen-3-ol(1.26%).

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Studies on the Development of Traditional Korean Golden Varnish(Hwangchil)(I) - Anatomical Characterisitics and Chemical Composition of Wood and Exudates of Hwangchil-namu(Dendropanax morbifera)- (전통 황칠 도료 개발에 관한 연구(I) - 황칠나무와 황칠액의 해부학적 특성과 화학적 조성-)

  • Lim, Kie-Pyo;Kim, Yoon-Soo;Chung, Woo-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.25 no.3
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    • pp.24-28
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    • 1997
  • As a way to revive the traditional technology of Korean golden varnish(Hwangchill), the exudates of D. morbifera, this study was carried out at first to investigate the anatomical characteristics and the chemical composition of D. morbifera stems and their exudates. Trees of more than 20 years old were harvested at Wando and Jejudo islands in the southwestern part of Korean peninsula during different physiological seasons of winter, spring, and summer, The results obtained are as follows: 1. In the anatomical aspect of wood, Hwangchil woods is ring-porous wood, has alternate inter-vessel pittings and horizontal intercellular canal in xylem. 2. In the chemical aspect of wood and bark, the general compositions appeared hot to differ from those of other hardwoods, and ash and alcohol-benzene extractives showed little increase with the increase of atmospheric temperature of harvesting season. 3. In the solvent-sequential extraction of bark, wood and exudate, the exudates was extracted up to 80% by ether, but the bark and wood contained a very small amount of ether extractives, about 2% in the bark and 0.5% in the xylem.

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Phytochemical Study for Botanical Utilization of the Fruits of Malus baccata (자원식물로서 응용을 위한 야광나무 열매의 식물화학적 연구)

  • Park, Hee-Juhn;Lee, Myung-Sun;Young, Han-Suk;Choi, Jae-Sue;Jung, Won-Tae
    • Korean Journal of Pharmacognosy
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    • v.24 no.4
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    • pp.282-288
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    • 1993
  • Very little utilization of the fruits of Malus baccata(Rosaceae) has been employed for food and medicinal plants except for preparing fruit beverages. But, it was estimated as valuable to investigate the chemical components for the botanical resource of this plant. In this study, it was found that the fruits of this plant contained primary long chain alcohol, ${\beta}-sitosterol$, campesterol, ursolic acid and ${\beta}-_D-glucosides$ of ${\beta}-sitosterol$ and campesterol. However, phloretin(dihydrochalcone) and its 5-O-glucoside(phloridzin) known as plant growth regulators in many Rosaceae plants were not found in this plant material by co-TLC analysis with authentic specimens. Although plant sex hormone, estrone, was often contained in relates of M. baccata, e.g., Prunus spp., Crataegus spp. and Malus spp., this compound was not detected in this fruit by comparison with an authentic material. By RIC chromatography, it was suggested that the Soxhlet extraction by the solvent of ether was excellently useful to extract ursolic acid efficiently.

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A Convenient Manufacturing Method for Mass Production of EGCG Rich Green Tea Extract (Epigallocatechin Gallate 고함유 녹차추출물의 제조공정 개선)

  • Seo, Eun Hye;Kim, Eun Jeong;Cheon, Seong Bong;Yoon, Min Ji;Choi, Sang Un;Ryu, Geon-Seek;Ryu, Shi Yong
    • Korean Journal of Pharmacognosy
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    • v.50 no.3
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    • pp.198-204
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    • 2019
  • A facile and convenient method was developed for the mass production of epigallocatechin gallate (EGCG) rich green tea extract (Er-GTE). The Er-GTE was successfully obtained from the crude water extract of green tea by the combination of two step purification, i.e., a simple adsorption process on the cation exchange resins (Trilite SCR-B) followed by the chromatography with Diaion HP-20 resins. The green tea extract produced by water extraction under $45^{\circ}C$ was subjected to adsorb on the strongly acidic cation exchange resin, Trilite SCR-B. The eluate passed through the resin was reabsorbed on Diaion HP-20 resin, which was subjected to elute with a mixture of water and alcohol by conventional chromatographical manner. The EGCG content in Er-GTE was estimated above 97% by HP-LC analysis and the newly developed method was regarded as the most suitable and appropriate process for the mass production of epigallocatechin gallate rich green tea extract (Er-GTE).

Analysis of the chemical burn-inducing components from the extraction of herb drug-mixed-medicine

  • Lee, Ju-Seon;Lim, Mie-Ae;Park, Hye-Young;Eo, Sang-Heui;Lee, Han-Sun;Park, Yoo-Sin
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.281.1-281.1
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    • 2002
  • Psoralen(7H-Furo[3.2-g] [1]benzopyran-7-one) and angelicin(2-Oxo-[2H]- furo[2.3-h]-1-benzopyran) are angular furocoumarin with diverse photobiological effects. They are major components of Psoralea corylifolia L.(破古紙). Psoralea corylifolia L. is used for a tonic and nursing one's energy. It can be also used for loss of virility. vitiligo. a skin disease, etc.. But a well known and often appreciated side effects f psoralens is the hyperpigmentation caused by this treatment. A women who used the herbal drug-mixed-medicine named'sobaeksu' to treat her vitiligo made a complaint against the orinetal medical doctor. She complained that her skin got burned to 2nd degree by the liquid. 'sobaeksu' through a medical celtficate. So we analyzed the components of that liquid with gas chromatography and gas chromatography/mass spectometry. It has 57.3% ethyl alcohol and two kinds of psoralens. Psoralens were psoralen and angelicin and each one of their contained quantity was 0.128mg/ml and 0.123mg/ml.

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Comparison of the Antioxidant and Physiological Activities of Different Solvent Extracts Derived from Enteromorpha compressa (납작파래(Enteromorpha compressa) 추출물의 항산화성 및 생리활성)

  • Ji-Won Choi;Sun-A Park;Won-Suk Kim;Yong-Tae Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.2
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    • pp.137-144
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    • 2024
  • In this study, we investigated the antioxidant and physiological activities of the Korean marine algae, Enteromorpha compressa. Solvent extracts of E. compressa were prepared using 70% ethanol, 80% methanol, and water, with extraction yields ranging from 9.55% to 25.67%. The total polyphenol and flavonoid contents ranged from 20.76-28.41 mg/g and 2.56-18.59 mg/g, respectively. Compared with the water extract, the ethanol and methanol extracts were found to have higher antioxidant activities. All three extracts were found to promote alcohol dehydrogenase and aldehyde dehydrogenase activities in a concentration-dependent manner, whereas the methanol and ethanol extracts were established to have the highest angiotensin-converting enzyme (ACE) inhibitory activity (IC50=1.40 ㎍/mL) and β-secretase inhibitory activity (IC50=0.17 ㎍/mL), respectively. These findings thus indicate that E. compressa could have beneficial application as a supplementary antioxidant and functional constituent in food and pharmaceutical materials.

Optimization of Extraction Conditions from Hericium erinaceus by Response Surface Methodology (반응표면분석법에 의한 노루궁뎅이 버섯 에탄올 추출조건의 최적화)

  • Choi, Mi-Ae;Park, Nan-Young;Woo, Seung-Mi;Jeong, Yong-Jin
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.777-782
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    • 2003
  • Response surface methodology (RSM) was used to monitor the characteristics of ethanol extracts from Hericium erinaceus. A central composite design was applied to investigate the effects of independent variables, ethanol concentration $(X_1)$, and sample ratio $(X_2)$ on dependent variables, soluble solid $(Y_1)$, total phenols $(Y_2)$, crude protein $(Y_3)$, electron donating ability $(Y_4)$, and browning color $(Y_5)$ of the extracts. As the sample ratio increased, the soluble solid content increased. Ethanol concentration played a minor role. Total phenols and crude protein increased with sample ratio. Sample ratio had a greater effect than alcohol concentration in the extraction of soluble solid, total phenols, crude protein, and browning color, with an exception of electron donating ability. The optimum ranges at 2 hr extraction was $3.9{\sim}5.0\;g/l00\;mL$ in sample ratio and $36{\sim}52%$ in ethanol concentration. Predicted values at the optimized conditions were acceptable when compared to experimental values.

Antioxidant capacities and β-glucan content of ethanol extract from Phellinus baumii (상황버섯(Phellinus baumii) 에탄올 추출물의 항산화능과 β-glucan 함량)

  • Bae, Hyun-Kyung;Hwang, In-Wook;Hong, Hee-Do;Chung, Shin-Kyo
    • Food Science and Preservation
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    • v.22 no.5
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    • pp.721-726
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    • 2015
  • Phellinus baumii has been used in traditional oriental medicine for the treatment of various cancer types, such as lung cancer, ovarian cancer and malignant melanoma. It has strong anti-cancer, anti-inflammatory and antioxidant activities due to its polysaccharides including glucan, schizophyllan, heteroglycan and lentinan, as well as its polyphenolics such as protocatechuic acid, caffeic acid, coumaric acid. ${\beta}$-Glucan and polyphenolics may be the most important activ ecompounds in P. baumii. Therefore, researchers have focused on these two compounds to improve their contents in extracts. In this study, P. baumii was extracted with hot-water and ethanol at different pH conditions, and their ${\beta}$-glucan contents, antioxidant activity and antioxidant contents were determined. Extraction yield was highest for the 60% ethanol extract at pH 4. The ${\beta}$-glucan contents of the hot-water extract at pH 7 was higher than those of the ethanol extracts. The antioxidant contents and antioxidant activities of the ethanol extracts were higher than those of the hot-water extracts. Extraction with 60% ethanol at pH 7 was appropriate with respect to the antioxidant capacities.

The Photoprotective Effects of Gynura Procumbens Against Photoaging: A Comparative Study Based on Extraction Methods (추출방법에 따른 명월초(Gynura Procumbens)의 광노화 완화 효과)

  • So Yeon Han;Da Yoon Lee;Ji Sun Moon;Hye Won Lee;Jae Hun Kim;Jae Ho Park
    • Korean Journal of Plant Resources
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    • v.37 no.2
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    • pp.101-109
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    • 2024
  • In this study, we investigated the antioxidant effects and active ingredients of Gynura Procumbens extracts obtained through various extraction methods for the development of natural-based cosmetics and pharmaceutical materials. The contents of compounds, total flavonoids, chlorogenic acid, and caffeic acid were compared at different concentrations, revealing the highest content of active ingredients in the 100% ethanol extract. Antioxidant activity assessed by DPPH and ABTS radical scavenging assays showed a concentration-dependent increase in antioxidant activity with the ethanol concentration. Additionally, we validated the DNA damage inhibition and anti-inflammatory effects of Gynura Procumbens extracts through UVB irradiation on Hs68 cell models. The 100% ethanol extract demonstrated significant inhibition of the expression of p-p53, γ-H2AX, iNOS, and COX-2 induced by UVB, indicating its potential in alleviating photodamage effects. Consequently, the efficient extraction of Gynura Procumbens for skin functional material development was confirmed, suggesting the suitability of ethanol or alcohol-based solvents.

Processing of Oleoresin Onion (양파 Oleoresin의 가공)

  • 최옥수;배태진
    • The Korean Journal of Food And Nutrition
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    • v.10 no.3
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    • pp.302-308
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    • 1997
  • The purpose of this study was to investigate the extraction yield and quality stability as to the oleoresin process with large amount of onion at one time. The first mixed-product is raw onion juice which was reduced the compression and concentrated by Brix 70% mixed together wit the residue which was extracted and concentrated by ethanol, the second product manufactured by the same method above after the autoclaving with onion, and the other product is made by grinding by 50mesh to freeze-dried onion. Each of yields were 7.3, 9.1 and 0.8% and each of total sugar content was 616.4, 712.3 and 150.3mg/g. Therefore the product extracted by ethanol from freeze-dried onion was very low in yield and total sugar content. By the index of the overall odor intensity, contents of total pyruvate were 1,733.7, 520.6, and 2,716.5$\mu\textrm{g}$/g for each product. As a result, oleoresin onion processing that desired to use raw onion was remarkable for odor recovery. For the homogenous mixture with concentrate of onion juice and ethanol extract were emulsified by the addition of 2% of PGDR(polyglycerol condensed ricinoleate) and agitation(10,000rpm, 30 minutes). At this time, interfacial tension was 1.9 dyne/cm and the formation of emulsion was for 96.2% when left over 24hours in 6$0^{\circ}C$. When it was to be centrifuged(2,000$\times$G, 80 minutes) after emulsification, the volume of emulsion level without seperation was 92.6%, and very high in emulsification stability. The induced heating-oxidize with soy bean oil and sesame oil added to 1% of onion oleoresin, induction-time extension effect appeared with antioxidant activity that was applicable for 80.8~82.2% as to the effect of addition of 0.02% BHA.

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