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A Convenient Manufacturing Method for Mass Production of EGCG Rich Green Tea Extract  

Seo, Eun Hye (AlnO Food Inc.)
Kim, Eun Jeong (AlnO Food Inc.)
Cheon, Seong Bong (AlnO Food Inc.)
Yoon, Min Ji (AlnO Food Inc.)
Choi, Sang Un (Korea Research Institute of Chemical Technology)
Ryu, Geon-Seek (AlnO Food Inc.)
Ryu, Shi Yong (Graduate School of New Drug Discovery and Development, Chungnam National University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.3, 2019 , pp. 198-204 More about this Journal
Abstract
A facile and convenient method was developed for the mass production of epigallocatechin gallate (EGCG) rich green tea extract (Er-GTE). The Er-GTE was successfully obtained from the crude water extract of green tea by the combination of two step purification, i.e., a simple adsorption process on the cation exchange resins (Trilite SCR-B) followed by the chromatography with Diaion HP-20 resins. The green tea extract produced by water extraction under $45^{\circ}C$ was subjected to adsorb on the strongly acidic cation exchange resin, Trilite SCR-B. The eluate passed through the resin was reabsorbed on Diaion HP-20 resin, which was subjected to elute with a mixture of water and alcohol by conventional chromatographical manner. The EGCG content in Er-GTE was estimated above 97% by HP-LC analysis and the newly developed method was regarded as the most suitable and appropriate process for the mass production of epigallocatechin gallate rich green tea extract (Er-GTE).
Keywords
Camellia sinensis; Epigallocatechin gallate; Green tea extract; Mass production;
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