Antioxidant capacities and β-glucan content of ethanol extract from Phellinus baumii

Phellinus baumii has been used in traditional oriental medicine for the treatment of various cancer types, such as lung cancer, ovarian cancer and malignant melanoma. It has strong anti-cancer, anti-inflammatory and antioxidant activities due to its polysaccharides including glucan, schizophyllan, heteroglycan and lentinan, as well as its polyphenolics such as protocatechuic acid, caffeic acid, coumaric acid. ${\beta}$-Glucan and polyphenolics may be the most important activ ecompounds in P. baumii. Therefore, researchers have focused on these two compounds to improve their contents in extracts. In this study, P. baumii was extracted with hot-water and ethanol at different pH conditions, and their ${\beta}$-glucan contents, antioxidant activity and antioxidant contents were determined. Extraction yield was highest for the 60% ethanol extract at pH 4. The ${\beta}$-glucan contents of the hot-water extract at pH 7 was higher than those of the ethanol extracts. The antioxidant contents and antioxidant activities of the ethanol extracts were higher than those of the hot-water extracts. Extraction with 60% ethanol at pH 7 was appropriate with respect to the antioxidant capacities.

상황버섯(Phellinus baumii) 에탄올 추출물의 항산화능과 β-glucan 함량

상황버섯을 $90^{\circ}C$에서 환류추출 시 시간과 추출용매의 조건(물 및 에탄올 농도, pH)에 따른 추출물을 제조하여 ${\beta}$-glucan 함량과 항산화활성 및 항산화성분의 함량을 조사하였다. 상황버섯 추출물은 추출시간이 길어짐에 따라 수율과 ${\beta}$-glucan 함량이 증가하여 24시간 추출 조건을 실험에 사용하였다. 추출용매에 따른 상황버섯 추출물의 수율은 60% 에탄올, pH 4의 조건에서 가장 높았다. ${\beta}$-glucan 함량은 열수 추출물에서 높은 함량을 나타내었고, 산성과 중성 조건에서 높게 나타났다. 항산화활성은 60% 에탄올, pH 7 조건에서 추출한 것이 가장 높았다. 항산화성분의 함량 또한 항산화활성과 같은 경향을 보였으며, 수율, ${\beta}$-glucan 함량, 항산화활성 및 성분을 모두 고려하였을 때 60% 에탄올, pH 7 추출 조건이 적합하였다.