• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2004년도 춘계 학술발표회
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• pp.166-166
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• 2004

• International Journal of Industrial Entomology and Biomaterials
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• 제30권2호
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• pp.64-74
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• 2015

Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.

• 한국환경농학회지
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• 제39권3호
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• pp.253-262
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• 2020

BACKGROUND: It has been reported that the nutritional composition of Locusta migratoria (Orthoptera: Acrididae) changes nutrients depending on the host plants. In this study, to confirm whether Locusta migratoria is an edible insect, the nutrients and harmful substances such as heavy metals and pathogens were analyzed and compared according to corn (LC) or wheat (LW) as host plants. METHODS AND RESULTS: All experimental methods mainly referred to AOAC (2004). The content of crude protein per dry weight was 77.3% in (LW), 1.1 times higher than 69.8% in LC. Crude fat was 6.5% in LW, 2.2 times less than LC 14.3%. Alpha-linolenic acid, which has the highest content among unsaturated fatty acids, was 1.2 times higher in LC (39.9%) than LW (32.5%). As a result of analysis of harmful substances by LC and LW, lead and cadmium among heavy metals were at levels suitable for heavy metal standards of edible insects, and arsenic was not detected in both groups. E. coli and Salmonella spp. were not detected in both groups. CONCLUSION: When comparing the overall nutrients composition of LW and LC, it was confirmed nutrients are different depending on the host plants, and the safety was proved.

• 식품과학과 산업
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• 제55권2호
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• pp.140-153
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• 2022

The purpose of this study is to organize research trends by analyzing healing studies using emotional insects, and to suggest future research directions. As a result, only six emotional insect species were used for healing purposes, and most of the age groups of the test subjects were elementary school students and the elderly. As for the type of research, field application of the healing program was dominant at 76.5%. Effectiveness verification was mainly conducted through questionnaires before and after participating in the healing program. In some cases, stress-related salivary hormones were used as indicators or clinical tests were conducted by medical staff. Based on the results of this analysis, future strategies such as finding new emotional insect species, developing various types of healing program models, and expanding and subdividing the target subjects were suggested to promote the growth of the healing industry using emotional insects in the future.

• 농촌지도와개발
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• 제25권2호
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• pp.99-110
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• 2018

Research confirms that healthful benefits accrued when people connect with nature and insect by viewing and interacting with them. Insect rearing is easy, relatively inexpensive, and can breed small space. This study aimed to investigate the physiological effects of animal assisted activity using insect(Oriental garden cricket) with diets and a rearing manual. The group of insect activity whose ages ranged from 65 to 82 years old that attending a community center in Daegu, Korea, were enrolled in the study between April and May 2014. The collected data was analyzed using qualitative analysis. Qualitative study is utilized to explore mental models, and their linguistic, affective, cognitive, social and cultural significance. The result showed that people-insect interactions promote well being of the elders, and the important aspect of insect activity aids in improvements in their social, emotional and cognitive functioning which were enhancing life satisfaction, reducing loneliness and increasing activities of daily life.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.70-74
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• 2015

The current market size of insect industry in Korea is estimated at 300 million dollars and more than 500 local farms are related to many insect industry. One of the strong candidates for insect industry is Korean horn beetle, Allomyrina dichotoma. Early this year, we reported a viral disease extremely fatal to A. dichotoma larvae. While we were proceeding a nationwide investigation of this disease, it was informed that similar disease symptom has been occurred occasionally during past over 10 years. The symptom can be easily confused with early stage of bacterial infection or physiological damage such as low temperature and high humidity. A peroral infection with the purified virus to healthy larvae produced a result that only 21% of larvae survived and became pupae. Although some of the survived adult beetle was deformational, many of them had no abnormal appearance and even succeeded in mating. Later, these beetles were examined if they were carrying the virus, and all except one were confirmed as live virus carrier. This implies that these beetles may fly out and spread the disease to the nature. We found the evidence for this possibility by collecting a few wild A. dichotoma larvae which were virus infected, near two local farms rearing A. dichotoma larvae. So far, transovarial transmission of this virus to the eggs, or horizontal transmission to other commercially reared insects is not known yet.

• 한국환경과학회지
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• 제32권11호
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• pp.833-840
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• 2023

Insect-healing program is designed for farm use, and this study attempted to scientifically investigate its healing effect based on application methods. The insects used in the program are Trypoxylus dichotomus, Papilio xuthus, and Bombyx mori. Insect-healing program was conducted on 61 adults of over 50 years old and at two different farms for 12 weeks. Stress, anxiety, insomnia, and cognitive function were measured through pre- and post-surveys. Result of the analysis showed that cognitive function changed positively in direct experienced group (those that encountered live insects) and indirect experience group (those that encountered insects only through photos and videos). However, psychological variables such as stress and anxiety decreased and sleep time increased significantly only in the direct experienced group. These results demonstrate that an agro-healing program that involve the use of insect must include activities such as raising and taking care of live insect to achieve positive psychological healing effects.

• Molecules and Cells
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• 제28권3호
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• pp.155-165
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• 2009

The newly sequenced complete mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys($St{\aa}l$) (Hemiptera: Pentatomidae), is a circular molecule of 16,518 bp with a total A+T content of 76.4% and two extensive repeat regions in A+T rich region. Nucleotide composition and codon usage of H. halys are about average when compared with values observed in 19 other published hemipteran mitochondrial genomes. Phylogenetic analyses using these 20 hemipteran mitochondrial genomes support the currently accepted hypothesis that suborders Heteroptera and Auchenorrhyncha form a monophyletic group. The mitochondrial gene arrangements of the 20 genomes are also consistent with our results.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.59-62
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• 2016

Many insects have gained increasing attention as an alternative protein for humans. Among those, the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae), was recently approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. For industrial utilization of G. bimaculatus, mass rearing techniques and production system should be standardized first. In this study, we investigated the effects of five different feeds on the growth and development of G. bimaculatus. Feed is the one of the key factors that has considerable effects on rearing insects. With five different kinds of feed on $1^{st}$, $3^{rd}$, and $5^{th}$ instar nymphs, the change of survival rate, body weight were monitored up to eight wk after hatching. We concluded that 50% of soybean flour, 20% of corn powder, 10% of rice bran, 9% of milk serum, 10% of rice flour, 0.5% of microorganisms, and 0.5% of multivitamins and minerals (diet C) was the best mix for promoting growth and development of $3^{rd}$ instar nymphs compared to the control diet.

• 한국응용곤충학회지
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• 제61권2호
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• pp.283-288
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• 2022

본 연구에서는 정서곤충을 이용한 치유프로그램 활동이 지역아동센터를 이용하는 아동 43명의 스트레스에 미치는 영향을 조사하였다. 치유프로그램에 이용된 정서곤충은 누에나방[Bombyx mori (Linnaeus)]이다. 스트레스의 정도는 타액 ɑ-아밀라아제 검사를 통해 측정하였다. 스트레스 수치를 평가하기 위한 타액 샘플 채취와 곤충 선호도에 대한 설문지는 곤충치유활동에 참여하기 전과 후에 측정되었고, 활동을 모두 마친 후에는 치유프로그램 참여 경험에 대한 만족도 점수를 평가하였다. 분석 결과, 곤충치유프로그램 활동은 참여 아동의 타액 ɑ-아밀라아제 수치를 통계적으로 낮추고, 곤충에 대한 선호도를 높여준다는 결과를 확인하였다. 누에를 이용한 치유프로그램 활동에 참가한 아동들의 만족도는 5점 만점에 4.23점이었다.