• 농촌지도와개발
• /
• 제20권2호
• /
• pp.383-405
• /
• 2013

본 연구의 목적은 농촌지도사의 직무별로 관련성 있는 역량을 도출하고자 하였으며 이를 위해 농촌지도사의 직무와 개별역량 간의 대응분석을 실시하였다. 분석결과 농촌지도사의 지도기획 직무는 '문제해결' 및 '아이디어 창출' 역량과 관련성이 높게 나타났으며, 생활개선 교육 직무는 '지도사업평가', '고객지향성' 및 '이해력' 역량과 관련성이 높게 나타났고, 작물기술보급 직무는 '현장지도' 및 '책임성' 역량과 관련성이 높은 것으로 분석되었다. 본 연구에서는 이러한 분석결과를 바탕으로 향후 농촌지도사들의 역량강화를 통한 직무의 효과성을 제고하기 위하여 직무별로 관련성이 높게 나타난 역량들에 대한 중점적인 교육이 실시될 필요성을 제시하였다. 또한 신규지도사의 최초 직무 배치시 개별역량의 측정 결과를 바탕으로 적정한 직무에 배치할 수 있는 기초자료로 활용하고자 하였다.

• Journal of Veterinary Science
• /
• 제21권2호
• /
• pp.26.1-26.14
• /
• 2020

Pancreatic ductal adenocarcinoma is a lethal cancer type that is associated with multiple gene mutations in somatic cells. Genetically engineered mouse is hardly applicable for developing a pancreatic cancer model, and the xenograft model poses a limitation in the reflection of early stage pancreatic cancer. Thus, in vivo somatic cell gene engineering with clustered regularly interspaced short palindromic repeats is drawing increasing attention for generating an animal model of pancreatic cancer. In this study, we selected Kras, Trp53, Ink4a, Smad4, and Brca2 as target genes, and applied Campylobacter jejuni Cas9 (CjCas9) and Streptococcus pyogens Cas9 (SpCas9) for developing pancreatic cancer using adeno associated virus (AAV) transduction. After confirming multifocal and diffuse transduction of AAV2, we generated SpCas9 overexpression mice, which exhibited high double-strand DNA breakage (DSB) in target genes and pancreatic intraepithelial neoplasia (PanIN) lesions with two AAV transductions; however, wild-type (WT) mice with three AAV transductions did not develop PanIN. Furthermore, small-sized Cjcas9 was applied to WT mice with two AAV system, which, in addition, developed high extensive DSB and PanIN lesions. Histological changes and expression of cancer markers such as Ki67, cytokeratin, Mucin5a, alpha smooth muscle actin in duct and islet cells were observed. In addition, the study revealed several findings such as 1) multiple DSB potential of AAV-CjCas9, 2) peri-ductal lymphocyte infiltration, 3) multi-focal cancer marker expression, and 4) requirement of > 12 months for initiation of PanIN in AAV mediated targeting. In this study, we present a useful tool for in vivo cancer modeling that would be applicable for other disease models as well.

• 대한지역사회영양학회지
• /
• 제12권4호
• /
• pp.489-500
• /
• 2007

The purpose of this study was to determine factors affecting intention to participate in school breakfast programs of middle and high school students in Seoul using the Theory of Planned Behavior. Out of 2,280 questionnaires distributed to the middle and high school students, 2,060 were returned (90.4% response rate) and 1,799 were analyzed (78.9% analysis rate). To determine factors affecting intention to participate in school breakfast programs, logistic regression analyses were conducted for middle and high school students, respectively. For logistic regression, data of 1,217 out of 1,799 students (637 middle and 580 high school students) were used after excluding 582 students which had an answer 'Not sure' to the question about intention to participate in school breakfast programs. In case of middle school students, male than female students (OR = 1.504), the students who skipped all breakfast (OR = 1.851), who ate breakfast $1{\sim}2$ times (OR = 3.474) or $3{\sim}4$ times (OR = 1.950) than those who ate breakfast everyday during weekdays of the previous week were more likely to participate in school breakfast programs. In case of high school students, male than female (OR = 1.967), the students who skipped all breakfast (OR = 4.187), the students who ate breakfast $1{\sim}2$ times (OR = 3.024) or $3{\sim}4$ times (OR = 2.095) than those who ate breakfast everyday during weekdays of the previous week were more likely to participate in school breakfast programs. In addition, both possibility of middle and high school students' participation in school breakfast programs increased as the satisfaction with school lunch service (OR = 1.704, 1.653) increased. Middle school students who perceived their household income level 'low or middle low' (OR = 1.999) than those who perceived their household income level 'middle' and the students who had more positive 'attitude' (OR = 1.311) toward eating breakfast were more likely to participate in school breakfast programs. However, high school students who had higher 'perceived difficulty in access to breakfast' (OR = 1.370) were more likely to participate in school breakfast programs. The results of this study could be useful data to plan and develop school breakfast programs in Korea.

• 한국환경교육학회지:환경교육
• /
• 제20권4호
• /
• pp.117-131
• /
• 2007

The objectives of this study were to investigate using environment-friendly agricultural products and to analyze consciousness of agricultural safety of consumers. The data were collected from 1,000 consumers by two-staged stratified random sampling. SPSSWIN/ver. 13 program was used for analyzing data with frequency, cross-tab, t-test and ANOVA. The major findings of this study were as follows: 1) Consumers with higher academic career, more income, and white collars showed higher experiences on environment-friendly agricultural products. 2) Consumers who are under thirties, university graduation and upper income revealed high intention to use environment-friendly agricultural products. 3) The study revealed information needs and information acquisition are significantly different among consumers 4) Consumers with higher academic and upper income showed higher agricultural safety concern, 5) The study discovered that consumers who are over sixties and under middle school graduation were higher on agricultural safety effects.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권11호
• /
• pp.1625-1633
• /
• 2003

This study was conducted to observe effects of dietary acidogenicity value (AV) on rumen fermentation characteristics and nutrients digestibility. The AV of feedstuffs was based on the dissolution of Ca from $CaCO_3$ powder added at the end of a 24 h in vitro fermentation. Three diets were formulated to be iso-energetic and iso-nitrogenous with different AV. Two experiments were involved in this study. In experiment 1, it appears that pH, $NH_3-N$ concentration and A:P ratio tended to decrease, but gas production, VFA production and DM disappearance tended to increase with increasing dietary AV. In experiment 2, the rumen pH tended to decrease in order of high AV>medium AV>low AV treatment, respectively. There were no significant effects of dietary AV on $NH_3-N$ concentration, enzyme activity and nutrient digestibility. In addition, total VFA and individual VFA concentrations tended to increase with increasing dietary AV without significance. In fact, we hypothesized that different dietary AV would affect rumen fermentation and nutrients digestibility because dietary AV was adjusted with fermentable carbohydrate sources. The present results indicate that differences in dietary AV between treatments were too small to affect rumen fermentation and its effects were minimal.

• 대한수의학회지
• /
• 제41권3호
• /
• pp.381-385
• /
• 2001

Neospora caninum infections have been associated with neonatal paresis as well as abortion around the world. Bovine abortion induced by N caninum was first reported in 1997 in Korea. Diagnosis of N caninum infection is usually based on histopathology and immunohistochemical detection of organism. However, often the tissues having lesion suggestive of N caninum infection were negative on immunohistochemistry. Here, we describe establishment of PCR-based diagnostic strategy for N caninum infection using DNA extracted from paraffin blocks containing the lesion. PCR was able to amplify N caninum-specific bands from the paraffin blocks containing at least moderate degree of inflammation. Compared to paraffin-blocks, DNA extracted from fresh tissues were less sensitive than that of paraffin blocks. This PCR-based method can be practically applicable for rapid diagnosis of bovine N caninum infection with high specificity and sensitivity. Based on this method, 17% of bovine abortion surveyed during a designated period was associated with N caninum infection.

• Journal of Microbiology and Biotechnology
• /
• 제14권2호
• /
• pp.417-421
• /
• 2004

A normal prion protein (PrPc) is converted to a protease resistant isoform (PrPsc) by an apparent self-propagating activity in bovine spongiform encephalopathies (BSE), which is a neurodegenerative disease. The cDNA encoding bovine PrP open reading frame (ORP) in Korean cattle was cloned by polymerase chain reaction (PCR). The cloned cDNA had a length of 795 base pairs which coded for a protein of 264 amino acid residues with a calculated molecular mass of 28.6 kDa. Identities of 90, 90, 79 and 78% on nucleotide and 94, 94, 84, and 84% on amino acid sequence were shown to PrP genes from sheep, goat, human, and mouse, respectively. The cloned DNA was ligated into the pQE30 expression vector and transformed into E. coli M15. The PrP was expressed by induction with isopropyl-$\beta$-D-thiogalactoside (IPTG) and purified on the Ni-NTA affinity column. High specific activities of the recombinant PrP were observed in the fraction of pH 5.8 eluate and showed a molecular mass of-29 kDa on SDS-PAGE and Western blot analysis.

• The Korean Journal of Ecology
• /
• 제26권2호
• /
• pp.65-70
• /
• 2003

Shihwa tidal freshwater marsh was constructed recently to treat pollutants entering Shihwa lake. In this study, we examined the spatial and temporal patterns of heavy metal accumulation in soils of Shihwa marsh and sought correlations between several soil variables (pH, electrical conductivity, organic matter, and acid ammonium oxalate-extractable Fe and Al contents) and the heavy metal concentration of soils. Surface soil samples (0∼20 cm) were collected in June 2000, November 2000, and July 2001, and were analyzed for heavy metals (Zn, Cd, Pb, Cu, Cr, As, and Hg) and soil chemical properties. The neutral pH and water-saturated conditions of Shihwa marsh appeared to favor immobilization of heavy metal through adsorption onto soils. The concentrations of heavy metal (especially Zn, Cu, and Cr) in soils of Shihwa marsh increased along the sampling occasions, suggesting that soils of Shihwa marsh serve as a sink of heavy metal. Among the sub-marshes, metal concentrations were highest in Banweol high marshes and lowest in Samhwa marshes. The temporal and spatial variations in the heavy metal concentrations of soils were correlated positively with organic matter and oxalate extractable Fe and Al contents, but negatively with electrical conductivity. These results suggest that organic matter and hydrous oxide of Fe/Al may playa key role in removing heavy metals in soils of Shihwa marsh, and that heavy metal removing capacity would increase with desalinization. However, the removal patterns of heavy metal by reeds warrant further studies to evaluate the total removal capacity of heavy metals by Shihwa marsh.

• 대한수의학회지
• /
• 제44권4호
• /
• pp.621-624
• /
• 2004

A 13-year-old female Miniature Pincher was euthanized after suffering from respiratory insufficiency and seizure. At necropsy, firm tan masses approsimately 4 to 5 cm in diameter were noted at the right caudal love and left cranial lobe of the lung. On cross sections of the cerebellum and the verebrum, several compressive firm round masses, 0.5 to 1 cm in diameter, were noted. similar looking nodules were also present in the mediastinal lymph nodes. Histopathologically, the neoplastic masses consisted of cuboidal shape epithelial cells that form glandular structure. The neoplastic cells have hyperchromatic nuclei with prominent nucleoli and moderate amount of cytoplasm. The degree of mitosis was high (4-6/400X). Multiple areas of necrosis, hemorrhage, and tumor emboli were also noted. Metastasis to the mediastinal lymph nodes, pancreas, and brain was confirmed. Based on the gross and histopathologic examinations, a diagnosis of primary metastatic pulmonary adenocarcinoma was made. Clinicaly recognized neurological signs were therefore due to brain metastasis.

• 한국학교보건학회지
• /
• 제19권2호
• /
• pp.37-47
• /
• 2006

Purpose : This study was to performed to investigate on the knowledge, atitues of Parents toward the use of food materials at school fod service and to provide schol mea l management with basic data to improvement of food purchasing guideline Methods rents who have children atending in elementary school or midle, high schol. The questionnaires we re administered from July to September, 2003. For the statistical analysis, SPS(12.0) was used to conduct th e descriptive analysis.Results : The results were as folows: seventy percent of the parents important. 'nutrition' and 'education' was next. Most parents wanted using food products produced in Korea for the safe and students' health. But 60% said it could not purcha se because of expensive price. About consuming regional agricultural products for school food service, 90% of parent welcomed.Conclusion quality and purchasing criteria ned to be developed for helping parent to do fod inspection. the parent should improve their knowledges on the food quality stadards. This result sugests that efective fod supply system in school food service of central goverment and autonomy also should be prepared.