• 제목/요약/키워드: after-school science program

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고령자를 대상으로 12주간 운동이 대요근 및 대퇴부 근황단면적에 미치는 영향 (Changes in Psoas Major and Quadriceps Cross Sectional Area in Elderly People after 12 Weeks of Exercise)

  • ;;;;;김준동;노호성
    • 생명과학회지
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    • 제21권1호
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    • pp.1-8
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    • 2011
  • 본 연구는 남녀 노인을 대상으로 12주간의 트레이닝을 실시한 후, 대요근과 대퇴부 근육의 근 횡단면적의 변화를 검토하였다. 연구 대상자는 65세에서 80세까지의 노인 58명(남성 30명, 여성 28명)이었으며, 대조군 19명과 운동군 39명으로 나누어 실시하였다. 측정항목은 신장, 체중, 체질량, 대요근과 대퇴부의 근 횡단면적을 측정하였다. 근육의 평가는 자기공명영상법(MRI)을 사용하여 실험 전, 후 실시하였다. 운동군은 대요근과 대퇴부 근육을 증가시키기 위하여 특별히 고안된 기계를 사용하여 평균 주 2일, 12주 동안 23번의 트레이닝을 실시하였다. 대요근의 측정부위는 L4와 L5의 중간부위를 분석하고, 대퇴부는 대퇴골과 무릎까지 길이를 나누어 50%부위에서 분석하였다. 트레이닝 후, 운동군의 대요근 근횡단면적은 9.4% 증가하였다(남성 11.5%, 여성 8.4%). 그러나 대퇴부 근 횡단면적은 남녀 모두 운동 후 증가경향은 나타나지 않았다. 또한 같은 기간 대조군의 근 횡단면적에서도 유의한 변화는 나타나지 않았다. 대요근은 자세 유지와 허리, 골반 및 대퇴부의 연결에 중요한 역할을 담당하기 때문에, 특히 노인의 이동활동에 중요하다고 사료된다. 본 연구에서는 새로이 개발된 트레이닝 기계를 사용하여 단기간트레이닝 후, 대요근 근 횡단면적의 증가를 확인하였으며, 이것은 노인들의 근력 및 이동능력의 향상 가능성이 시사되었다.

Co-authorship patterns and networks of Korean radiation oncologists

  • Choi, Jin-Hyun;Kang, Jin-Oh;Park, Seo-Hyun;Kim, Sang-Ki
    • Radiation Oncology Journal
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    • 제29권3호
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    • pp.164-173
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    • 2011
  • Purpose: This research aimed to analyze the patterns of co-authorship network among the Korean radiation oncologists and to identify attributing factors for the formation of networks. Materials and Methods: A total of 1,447 articles including contents of ‘Radiation Oncology' and 'Therapeutic Radiology' were searched from the KoreaMed database. The co-authorship was assorted by the author's full name, affiliation and specialties. UCINET 6.0 was used to fi gure out the author's network centrality and the cluster analysis, and KeyPlayer 1.44 program was used to get a result of key player index. Sociogram was analyzed with the Netdraw 2.090. The statistical comparison was performed by a t-test and ANOVA using SPSS 16.0 with p-value < 0.05 as the significant value. Results: The number of articles written by a radiation oncologist as the first author was 1,025 out of 1,447. The pattern of coauthorship was classified into five groups. For articles of which the first author was a radiation oncologist, the number of singleauthor articles (type-A) was 81; single-institution articles (type-B) was 687; and multiple-author articles (type-C) was 257. For the articles which radiation oncologists participated in as a co-author, the number of single-institution articles (type-D) was 280 while multiple-institution articles (type-E) were 142. There were 8,895 authors from 1,366 co-authored articles, thus the average number of authors per article was 6.51. It was 5.73 for type-B, 6.44 for type-C, 7.90 for type-D, and 7.67 for type-E (p = 0.000) in the average number of authors per article. The number of authors for articles from the hospitals published more than 100 articles was 7.23 while form others was 5.94 (p = 0.005). Its number was 5.94 and 7.16 for the articles published before and after 2001 (p = 0.000). The articles written by a radiation oncologist as the first author had 5.92 authors while others for 7.82 (p = 0.025). Its number was 5.57 and 7.71 for the Journal of the Korean Society for Therapeutic Radiology and Oncology and others (p = 0.000), respectively. Among the analysis, a significant difference in the average number of author per article was indicated. The out-degree centrality of network among authors was 4.26% (2.03-7.09%) while in-degree centrality was 1.31% (0.53-2.84%). The three significant nodes were classified and listed as following: Choi, Eun Kyung for 1991-1995, Kim, Dae Young for 1998-2001, Park, Won and Lee, Sang Wook for 2003-2010. Choi, Eun Kyung and Kim, Dae Young appeared in two cases, and ranked as the highest degree in centrality. In the key player analysis, Choi, Eun Kyung and Lee, Sang Wook appeared in two cases, and ranked as the highest. From the cluster analysis, Sungkyunkwan University, Seoul National University and Yonsei University revealed as the three large clusters when Ulsan University, Chonnam National University, and Korea Institute of Radiological & Medical Science as the medium clusters. Conclusion: The Korean radiation oncologist's society shows a closed network with numerous relationships among the particular clusters, and the result indicates it is different from other institutions in the pattern of co-authorship formation of the major hospitals.

보건행정 전공 대학생들의 병원실습 만족도에 영향을 미치는 요인분석 (Factors Analysis Related to Health Administration Students' Satisfaction on Hospital Practice)

  • 박은영;장영진;홍종필
    • 한국임상보건과학회지
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    • 제5권1호
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    • pp.825-833
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    • 2017
  • Purpose .This study aims to assess the influence factors on the level of satisfaction with hospital training and to provide basic data for more efficient hospital practice of students' public health administration . Methods . We have conducted a survey on students satisfaction with their hands on practice against 200 students in Busan, Kyeongnam and Kyungbook area, who have completed their practice in public health administration. We have identified the general characteristics of the target respondents, the current training status of the department for the hospital practice and training venue; also analyzed the characteristics composed of the apprentice student's attitude before the practice, satisfaction with the department training program and hospital for practice, as well as their practice at hospital. The general characteristics of the target respondents and clinic practice status at the hospital were measured in percentage and frequency analysis; the average and standard deviations of hospital size were also measured; and the correlations between satisfaction with the department, attitude towards the practice and satisfaction with the hospital were analyzed. Results . Most public health administration students experienced their practice at the hospital after the first semester on the $2^{nd}$year and they have received the foundation training for the practice. The survey allowed multiple responses and the results are as follows: medical terminology 49.5%, medical coordinator 36.7%, Hospital administration 26.5%, Health insurance 17.3%, and medical recording and practice 13.8% respectively. In terms of each individual student's attitude towards the practice at the hospital, the respondents answered as follows: I have completed the obligatory subjects for the practice before the practice 89.5%; I have been fully informed on the hospital for my practice. 74.5%; I responsibly practiced and worked with professionalism and sense of belonging to the hospital as a member of staff 90%; I have actively learned and adopted 95%; My knowledge and skills from the practice was relevant to the theory and practice from my course 83.5%. The findings of satisfaction by size of hospital were in order of a small clinic($3.24{\pm}0.63$), medium sized clinic($3.27{\pm}0.65$), hospital ($3.20{\pm}0.61$), and large sized comprehensive hospital ($2.93{\pm}0.74$). Consequently the satisfaction rate shows no significant difference by size of hospital. In relations between practice attitude and satisfaction with subject, the matter of acquiring information and fully understanding of the hospital for practice before practice(p=0.04), the matter of a sense of belonging and responsibility at hospital during practice(p=0.33). the matter of active adaptation attitude during practice (p=0.42), and the matter of correlations with school curriculum during practice(p=0.00) showed significant results. In relations between practice attitude and satisfaction with the hospital for practice, the matter of acquiring information and fully understanding of the hospital for practice before practice(p=0.01), the matter of a sense of belonging and responsibility at hospital during practice(p=0.04), and the matter of correlations with school curriculum during practice(p=0.00) showed significant results. Conclusion . The most essential subject for the practice in public health administration is medical terminology and there is no significance in satisfaction with the practice by size of hospital. Students were content with the hospital where they can experience as much as possible. Students content with their major show positive attitude towards the practice and so do those content with the hospital. Those with the positive attitude towards the practice show the high correlation of satisfaction with both the major and hospital. As a result, the satisfaction with the major is the significant attribute to the practice in the hospital.

도시 생태공원 이용자 특성 연구 - 길동생태공원, 여의도샛강생태공원을 사례로 - (The Visitors Characteristics of Urban Ecological Park - The Cases of Gildong Ecological Park, Yeouido Tributary Ecological Park -)

  • 김은영;이정아;김형곤;전진형
    • 한국조경학회지
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    • 제42권1호
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    • pp.64-74
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    • 2014
  • 생태공원은 자연 자원의 지속 가능한 이용을 활성화하고 대상지를 기반으로 환경 교육을 위한 장소로서 활용될 수 있다. 그러나 생태공원에서 운영되는 생태 체험 프로그램은 이용자들이 직접 체험하고 관찰하는 방식의 교육으로 이용자들이 요구하는 여가편익을 반영하여야 하지만, 이용자들을 대상으로 생태공원에 대한 여가편익을 심층적으로 고찰한 연구는 미비한 실정이다. 이에 본 연구에서는 생태공원을 방문하는 이용자들의 환경태도, 여가편익, 만족도 간의 영향관계를 파악하고자 하였다. 실증적 분석을 위해 서울시에 위치한 길동생태공원, 여의도 샛강 생태공원의 이용자를 대상으로 총 282부의 유효한 설문지를 수집하였다. 통계적 분석결과, 생태공원을 방문하는 이용자들의 환경에 대한 태도가 긍정적일수록 이용자들의 생태공원 방문시 자연친화적 경험에 대한 교육과 전문적인 지식 확대에 대한 여가편익에 높은 기대를 하고 방문하는 것으로 나타났다. 생태공원을 통해 이용자들이 원하는 여가편익이 방문하기 전 기대와 방문 후 여가편익의 차이 분석을 실시한 결과, 이용자들이 생태공원을 방문할 가치가 있었다고 느꼈으나, 교육적인 측면의 여가편익에서는 방문 전 기대보다 불충분한 만족을 느끼고 있음을 나타냈다. 또한, 방문 후 여가편익은 생태공원 전체 만족도에 긍정적인 영향을 주고, 긍정적인 환경에 대한 태도는 전체 방문만족도에 영향을 미치지 못하는 것으로 나타났다. 이는 현재 우리나라의 생태공원이 일반 공원과 차이를 보이지 않고, 공원을 방문하는 이용자들도 차이를 느끼지 못하기 때문이라 판단된다. 이러한 연구결과는 향후 생태공원 이용자들이 요구하는 여가편익인 자연친화적 경험과 생태교육의 다양성을 중심으로의 일반공원과의 차별적인 관리가 필요하다.

임상간호원에 대한 연구조사 (A Study and Survey on Clinical Nurses concerning the General Items, the Motives of Determining their Profession, the Attitudes toward their Profession and the Desire and Expectation to their Profession and Society)

  • 이귀향;우옥자;서문자
    • 대한간호학회지
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    • 제3권3호
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    • pp.78-96
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    • 1973
  • This study of 855 clinical nurses was conducted using a questionnaire that include tour different scales; the motives of determining their profession, the attitudes toward their profession, the general items, and desire and expectation to their profession and society. The data were analyzed by Chi-Square Test and Percentage. The results of this study included Hypothesis are as follows; The respondents were 855 (78.6%) among 1088 clinical nurses who were employed by General of Educational hospitals through the city of Seoul, Pusan, Taegu, Daejun, Kwangju, and Wonju. 1) a. In the Age Distribution, the majority of respondents were under the age of 30yrs(88.2%) and the minority were above 31yrs(11.2%), and the preponderance of the majority to minority(9:1) was noted. In compared with area, a group above 31yrs old in Seoul (6.9%) was lower than other area (16.3%). b. The types of Educational background were 16.3% in Voc.Tr. School, 66.5% in Diploma and 17.1% in Degree.146 clinical nurses were from the Degree course, and 142 (97.3%) CN among those of them were occupied around Seoul and 4(2.7%) around other area. c. In the Marital Status,71,5% were the unmarried and 28.5% were the married. And compared with the area was 20.4% in Seoul and 41.4% in other area. d. Most common Length of Clinical Experience after graduation was under tile 2yrs (55.4%), 3yrs(14.2%, and 4yrs (6.2%). In compared with area, Seoul (15.3%) was lower than other area (38.1%) above 5yrs of clinical experience, and the preponderance of tile other area to Seoul as 2.5: I was noted. 2) a. Hypothesis 1 was significant relation between the types of Educational Background of the CN and their motives for selection of Nursing, P-value was below 0.01. b. There was a significance on hypothesis 2 (P<0.01): that was relation between their motives for selection of clinical nursing field after their graduation and the area which they were employed. c. Hypothesis 4 was accepted as significant relation between the level of satisfaction of their clinical experience after their graduation and the types of educational back ground, P-value was below 0.01. d. There was a significance on hypothesis 5(P<0.01) that was relation between the CN's response about the orientation program and the area which they were employed. e. Hypothesis 6 was retained as significant relation between the area and inservice educational programme of their employed hospital was practising or not. P-value was 0.01. f. Hypothesis 7 was retained as significant relation between the area and the CN's response about the inservice educational programme of their employed. P-value was below 0.01. g. There was a significance on hypothesis 8 (P<0.0l) that was relation between the CN's experience on attending the professional meeting and the area. h. Hypothesis 10 was accepted as significant relation between the response about the present licence system and their educational background. p-value was below 0.01. i. There was a significance on hypothesis 11 (P fO.01) that was relation between the carrying out the regular and delivery vacation and the area. J. Hypothesis 12 was accepted as significant relation between the CN's consideration of the lack of leisure and their marital status. p-value was below 0.01' k. There was a significance on hypothesis 13 (P <0.01) that was relation between the CN's response about their salary and their marital status. l. Hypothesis 14 was significant relation between the most difficulties of CN during their working and the hospital which they were employed. p-value was below 0.01.

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척수장애 기혼남성의 성 적응과 삶의 질에 관한 연구 (A Study on the Sexual Adjustment and Quality of Life in Married Men with Spinal cord Injuries)

  • 김영희;조복희
    • 재활간호학회지
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    • 제3권1호
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    • pp.27-42
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    • 2000
  • The purpose of this study was to identify the degree of sexual adjustment and quality of life in married men with spinal cord injuries and to furnish primary data to sexuality rehabilitation nursing practice which can lead to ideal sexual life and quality of life those men with SCI. Seventy married men with SCI were conveniently sampled in Kwangju, Suncheon, Mokpo and Seoul for responding the questionnaire, which was based on this research. With the research scale, SIS (Sexual interest and satisfaction) by Siosteen et al.(1990) and SB(Sexual behaviour) by Kreuter et al.(1996) were used for the measurement of sexual adjustment. And, for measuring quality of life was used SCI QL-23(Spinal cord injury of life-23) scale by Lundqvist et al.(1997). Data were collected from January 20 to March 20, 1999, using a structured questionnaire. A hundred volumes of questionnaire were used. and 85 volumes were collected, 70 volumes were used as research data after excluding 15 volumes unsuitable to data analysis. The obtain data were analysed using percentage, t-test, ANOVA, Duncan test, and Pearson's correlation by SAS PC+ program. The results were as follows: 1. The mean score of sexual interest and satisfaction of the subjects was 8.42. out of 18. The ways of their sexual behavior after spinal cord injuries were embracing and caressing (62.9%), kissing(58.6%), caressing breast with hands (55.7%), caressing breast with hands (52.9%), caressing genitals with hands (37.2%), caressing genitals with mouth(30.0%) and sexual intercourse (18.6%). The mean score of quality of life was 52.53 out of 100. 2. Age(F=3.24, p=.045) and caregiver (F=4.02, p=.022)were major variables which reveals significant differences in terms for sexual interest and satisfaction. The later results on Duncan's test showed that subjects who were in their 30s or 40s were significant higher than subjects whose age were in their 50s in their sexual interest and satisfaction. Also subjects with their spouse's care or mother's care were higher than those with other's care. 3. Subjects with incomplete paraplegia were higher than those with the complete paraplegia in sexual interest and satisfaction (F=3.01, p=.036). 4. Variables that showed the significant differences in the quality of life were education(t=2.860, p=.007) and period of marriage(t=2.125, p=.037). and occupational status(t=-2.161, p=.034). High school graduates, those who married before spinal cord injuries and those who didn't have occupation were higher than the other subjects. 5. Variables that revealed significant differences in the quality of life were time passage after spinal cord injuries(F=8.72, p=.001) and injured level of spinal cord(F=3.32, p=.042). Duncan's test showed that subjects who had lived for less than 4 years were higher those with time passage of 5-9 years and 10 years. Also subjects with lumbar injuries were higher than those with thoracic injuries in terms of quality of life. 6. There was negatively correlated between sexual interest and satisfaction and quality of life(r=-.256, p<.05). As a result of these findings sexuality rehabilitation for individuals with SCI was very important issue for their quality of life. Thus, registered nurses who care clients with SCI should activily participate in the client's sexual needs. Also, various sexual behaviors as well as sexual intercourse should be encouraged for the sexual adjustment of client's with SCI.

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한국과 중국 간호대학생의 죽음에 대한 의식 (The Death Orientation of nursing students in Korea and China)

  • 이진숙;최화숙
    • 호스피스학술지
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    • 제8권1호
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    • pp.1-12
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    • 2008
  • 목적: 한국과 중국 간호대학생의 죽음의식을 조사하여 죽음교육과정설계에 간호대학생의 특성을 고려한 내용이 반영되도록 기여하고 간호교육 및 임상간호의 질적 향상에 도움이 되는 자료를 제공하고자 하였다. 방법: 본 연구는 2007년 3월19일부터 3월23일 사이에 한국 E대학교 간호대학생 248명과 중국 Y대학교 간호대학생 244명 총 492명을 대상으로 하였으며 측정도구는 Thorson과 Powell(1988)의 죽음의식도구를 박석춘(1992)이 번안하여 사용했던 도구를 이용하였다. 수집된 자료는 SPSS(12.0)통계 프로그램을 이용하여 전산처리 하였으며, 실수, 백분율, 평균, 표준편차는 기술통계 방법을 사용하고, Cronbach Alpha Coefficient, 요인분석, t-test, ANOVA 및 회귀분석 등을 통하여 분석하였다. 결과: 1. 한국 간호대학생은 반수이상(58.5%)이 종교가 있는데 비해 중국 간호대학생은 거의 종교를 가지고 있지 않았다(93.9%). 죽음경험이 한국 간호대학생(66.9%)보다는 중국 간호대학생이 많았다(76.6%). 사후세계유형에서 한국 간호대학생은 '사람이 죽으면 천국이나 지옥중의 한곳으로 간다'고 생각하는 경우가 제일 많았고(27.3%), '사람이 죽으면 어떻게 될 것인지 통 모르겠다'가 2위로 나타났다(22.9%). 중국 간호대학생은 '사람이 죽으면 어떻게 될 것인지 통 모르겠다'가 제일 많고(30.3%), '사람이 죽으면 그것으로 끝이고 사후세계는 존재하지 않는다'가 그 다음으로 많았다(29.5%). 2. 죽음의식은 한국 간호대학생이 평균 2.36점, 중국 간호대학생이 평균 2.50점으로 죽음에 대한 염려, 불안, 두려움이 중간수준이었으며 중국 간호대학생이 한국 간호대학생보다 높았다(t=3.51,p=.000). 3. 한국 간호대학생의 죽음의식은 연령(t=3.20, p=.002), 종교(t=2.56, p=.011), 사후세계(F=4.64, p=.000)에 대한 태도에 따라 유의한 차이가 나타났으며, 죽음의식에 대한 기여도는 사후세계변수가 수량화범위 0.735(p=.000)로 나타났다. 중국 간호대학생의 죽음의식은 모든 변수에서 유의한 차이가 없는 것으로 나타났다. 결론: 본 연구의 결과, 내세에 대한 믿음을 가지고 있는 대상자의 죽음의식이 제일 긍정적이므로 영적간호의 필요성이 강조된다. 한편 중국 간호대학생은 한국 간호대학생보다 죽음을 더 의식하고 죽음에 대한 염려, 불안, 두려움이 더 높게 나타났으므로 간호교육과정의 비교검토를 통해 한국의 죽음교육내용을 참고하고 중국의 정치, 문화와 사회제도에 알맞은 죽음교육을 해야 된다고 하겠다. 죽음의식은 사후세계에 대한 태도가 가장 크게 영향을 미치고 있으므로 이에 대한 지속적인 연구와 교육 및 실무에 적용을 제언한다.

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동계훈련 시 산수유 추출물 섭취가 남중 축구선수의 혈중 피로물질, 근 손상 및 간 기능에 미치는 영향 (Effects of Cornus Officinalis Extract on Blood Fatigue Substance, Muscle Damage and Liver Function during Winter Training in Middle School Male Soccer Players)

  • 박성환;하수민;하민성;백영호
    • 한국응용과학기술학회지
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    • 제34권4호
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    • pp.827-838
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    • 2017
  • 본 연구는 4주간의 동계훈련기간 중 산수유 추출물을 섭취시켜 간 기능, 근 손상 및 혈중 피로물질에 미치는 영향을 구명하기 위하여 남중 축구선수 20명을 대상으로 동계훈련+산수유 추출물 섭취군(10명), 동계훈련군(10명)으로 구분하여 실시하였다. 동계훈련은 1일 오전, 오후, 야간운동으로 구분하여 주 6일, 4주간 실시되었으며, 운동강도는 70-95%HRR로 수행되었다. 산수유 추출물은 매일 아침과 저녁 식사 후 각각 80 mL(1일 160mL)를 4주간 섭취시켰으며, 측정된 자료의 그룹 내 변화는 대응표본 T검정, 그룹간 차이는 변화율을 산출 한 뒤 독립표본 T검정을 이용하여 비교 분석하였다. 그 결과 혈중 피로물질의 젖산은 동계훈련+산수유 추출물 섭취군에서 유의하게 감소하였으며(p<.05), 동계훈련군 보다 동계훈련+산수유 추출물 섭취군이 유의하게 감소하였다(p<.05). 암모니아는 동계훈련+산수유 추출물 섭취군(p<.05), 동계훈련군(p<.001) 모두 유의하게 증가하였다. 근 손상지표의 CK는 동계훈련+산수유 추출물 섭취군이 유의하게 감소하였으며(p<.05), LDH는 동계훈련+산수유 추출물 섭취군(p<.001), 동계훈련군(p<.05) 모두 유의하게 감소하였다. 간 기능 효소 중 AST(p<.01), ALT(p<.05)는 동계훈련+산수유 추출물 섭취군이 유의하게 감소하였으며, AST는 동계훈련군 보다 동계훈련+산수유 추출물 섭취군이 유의하게 감소하였다. 동계훈련 시 피로에 쉽게 노출 될 수 있는 선수들에게 있어 산수유 추출물 섭취는 항피로의 역할을 하는데 긍정적인 영향을 미치는 것으로 사료된다.

일부 치과병원 흡연환자의 구강위생용품 이용도 (Availability of Oral Hygiene Devices for the Patients with Smoking in some Dental Clinics)

  • 장경애;성미경;강현경;최정옥;김윤신
    • 치위생과학회지
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    • 제8권1호
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    • pp.7-12
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    • 2008
  • 본 연구는 흡연유무에 따른 구강위생용품의 이용도를 파악하여 구강건강을 증진시키고 금연활동지도를 위해 일부 치과병원에 내원한 326명을 대상으로 자기기입식 설문조사를 실시하였다. 수집된 자료는 SPSS 12.0 통계프로그램을 이용하여 다음과 같은 결론를 얻었다. 1. 흡연유무에 있어 사용하는 잇솔의 종류는 '부드러운 잇솔' 이라는 응답은 흡연자에서 64.5%로 높게 나타났고(p < 0.001), 1회 잇솔질 시간은 '3분이상' 이라는 응답은 비흡연자가 61.4%로 높게 나타났다(p < 0.001). 2. 흡연유무에 있어 사용하는 구강위생용품의 종류는 비흡연자는 36.7%로 흡연자보다 치간잇솔 사용이 더 높게 나타났다. 3. 흡연유무에 있어 구강위생용품을 사용하게 된 동기는 '치과에서 권장해서' 라고 응답한 비흡연자와 흡연자는 각각 69.6%, 67.5%로 비슷하였고, 구강위생용품을 사용하지 않는 이유는 '사용법을 몰라서' 라고 응답한 흡연자는 38.7%로 비흡연자보다 높게 나타났다(p < 0.05). 4. 흡연유무에 있어 구강건강관리방법은 '정기적으로 치과에서 검진한다' 라고 응답 한 흡연자는 28.3%이었고, 비흡연자는 35.3%로 비흡연자가 약간 높게 나타났다. 본 연구의 결과, 흡연의 유무에 따른 구강위생용품의 이용도와 인지는 흡연자가 낮았으며, 유의한 차이를 보였다. 따라서 전국민을 대상으로 구강위생용품의 효과 및 필요성에 대한 홍보가 적극적으로 이루어지고, 치과의료기관에서는 구강구강보건 전문인력인 치과위생사는 흡연유무에 있어 구강의 특성을 파악하여 적합한 구강위생용품의 선택과 사용방법에 대한 교육과 금연지도활동이 치과에서 확대실시 될 수 있도록 노력하여야 할 것이다.

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인간 제대혈액에서 유래된 중간엽 줄기세포의 신경 및 콜린성 분화 (Neural and Cholinergic Differentiation of Mesenchymal Stem Cells Derived from the Human Umbilical Cord Blood)

  • 감경윤;강지혜;도병록;김해권;강성구
    • 한국발생생물학회지:발생과생식
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    • 제11권3호
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    • pp.235-243
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    • 2007
  • 인간 제대혈 세포는 조혈모세포, 중간엽 줄기세포와내피전구세포를 풍부하게 포함하고 있다. 인간 제대혈 속의 중간엽 줄기세포는 조혈모세포와는 달리 다능성 줄기세포이며 신경세포로 분화할 수 있는 잠재성을 가지고 있다. 본 연구에서는 세포배양을 통해 제대혈의 중간엽 줄기세포를 신경세포와 콜린성 신경세포로 분화를 유도하였다. 중간엽 줄기세포를 신경세포로 분화시키기 위해 배양액에 dimethyl sulphoxide(DMSO)와 butylated hydroxyanisole(BHA)를 첨가하여 유도하였으며 basic fibroblast growth factor(bFGF), retinoic acid(RA), sonic hedgehog(Shh)를 처리하여 콜린성 신경세포로 분화시켰다. DMSO와 BHA에 처리된 중간엽 줄기세포가 빠르게 신경세포 모양으로 분화하는 것을 관찰하였으며, 이것은 면역조직학적 염색에서 신경세포 특이 표지인 $\beta$-tubulin III, 별아교세포에 대한 특이 표지인 GFAP, 희돌기아교세포에 대한 특이 표지인 Gal-C에 대해 양성반응을 나타내었고, 그 비율은 각각 $32.3{\pm}2.9%$, $11.0{\pm}0.9%,\;9.4{\pm}1.0%$였다. RT-PCR 분석에서 배양 단계에 따라 신경세포에 특이적인 표지 인자가 발현됨을 통해, 중간엽 줄기세포가 신경세포로 분화됨을 확인하였다. 또한, 중간엽 줄기세포에 bFGF, RA, Shh를 처리하여 콜린성 신경세포로 분화시켰을 때, 전체 중간엽 세포 중 $31.3{\pm}3.2%$가 신경세포 특이 표지인 $\beta$-tubulin III에 양성반응을 보였으며 이들 세포 중 $70.0{\pm}7.8%$가 콜린성 신경 특이 표지인 ChAT에 양성반응을 보였고, 이것은 Woodbury 방법에 의한 신경분화의 경우보다 3배 가량 높은 비율로 콜린성 신경의 분화를 유도한 것이다. 이러한 실험 결과들은 인간 제대혈의 중간엽 줄기세포가 콜린성 신경세포로 분화가 가능하고 이러한 잠재성을 가진 제대혈 중간엽 줄기세포는 퇴행성 신경질환에 대한 세포 치료제로서 가능성을 제시한다.

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