• BMB Reports
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• 제49권11호
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• pp.612-616
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• 2016

CD44 pre-mRNA includes 20 exons, of which exons 1-5 ($C_1-C_5$) and exons 16-20 ($C_6-C_{10}$) are constant exons, whereas exons 6-15 ($V_1-V_{10}$) are variant exons. $V_6$-exon-containing isoforms have been known to be implicated in tumor cell invasion and metastasis. In the present study, we performed a SR protein screen for CD44 $V_6$ splicing using overexpression and lentivirus-mediated shRNA treatment. Using a CD44 $V_6$ minigene, we demonstrate that increased SRSF3 and SRSF4 expression do not affect $V_6$ splicing, but increased expression of SRSF1, SRSF6 and SRSF9 significantly inhibit $V_6$ splicing. In addition, using a constitutive exon-specific primer set, we could not detect alterations of CD44 splicing after SR protein-targeting shRNA treatment. However, using a $V_6$ specific primer, we identified that reduced SRSF2 expression significantly reduced the $V_6$ isoform, but increased $V_{6-10}$ and $V_{6,8-10}$ isoforms. Our results indicate that SR proteins are important regulatory proteins for CD44 $V_6$ splicing.

• 한국재료학회지
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• 제33권11호
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• pp.447-457
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• 2023

Single-atom Pd clusters anchored on t-BaTiO₃ material was synthesized using hydrothermal and ultrasonic methods for the effective piezoelectric catalytic degradation of pollutants using vibration energy. XRD patterns of BaTiO₃ loaded with monoatomic Pd were obtained before and after calcining, and showed typical cubic-phase BTO. TEM and HAADF-STEM images indicated single-atom Pd clusters were successfully introduced into the BaTiO₃. The piezoelectric current density of the prepared Pd-BaTiO₃ binary composite was significantly higher than that of the pristine BaTiO₃. Under mechanical vibration, the nanomaterial exhibited a tetracycline decomposition rate of ~95 % within 7 h, which is much higher than the degradation rate of 56.7 % observed with pure BaTiO₃. Many of the piezo-induced electrons escaped to the Pd-doped BaTiO₃ interface because of Pd's excellent conductivity. Single-atom Pd clusters help promote the separation of the piezo-induced electrons, thereby achieving synergistic catalysis. This work demonstrates the feasibility of combining ultrasonic technology with the piezoelectric effect and provides a promising strategy for the development of ultrasonic and piezoelectric materials.

• 아동학회지
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• 제30권2호
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• pp.129-144
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• 2009

This study investigated Korean elementary school children's happiness by gender and grade and by attendance in after-school programs during weekdays and weekends. Participants were 3,608 children in Korean provincial areas except Jeju-do; they responded to the Happiness Scale developed by the researchers. Data were analyzed by frequency, percent, and t-test. Results were (1) children's happiness level was high; girls' happiness level was higher than boys'. As they moved up in school grade, happiness level decreased. (2) Their attendance in after-school programs during weekdays and weekends decreased their happiness level in leisure and extra academic activity, but it increased happiness in school/community life and sibling relations. Conclusions were that reduction in the amount of children's after-school programming would increase children's happiness.

• 한국간호교육학회지
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• 제17권3호
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• pp.487-500
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• 2011

Purpose: The purpose of this study was to develop a finding meaning in life CD program about life-esteemed education and to identify the effect of the program. Methods: The life-esteemed education philosophy and the concepts of logotherapy were applied as a theoretical framework for this program. This program was developed through the process of planning, designing, developing, and evaluating with a content validity test. To identify the effect of the program, one experimental group design was applied to 54 students. Data were collected before the program started and one week and five weeks after the program finished. Results: The program was developed based on the students' needs and evaluation of the CD's content and consists of five periods: Dinosaur Park of Promise, Hill of Fragrance, Garden of Love, Forest of Acceptance, and My Lake. Each post-test score of knowledge, attitude, and practice on meaning of life was significantly higher than pretest scores. Conclusion: This program can be effective for life-esteemed education in elementary school students. Moreover, it is encouraged that the program will be utilized in more life-esteemed education for elementary school students.

• 한국가정과교육학회지
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• 제17권1호
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• pp.1-13
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• 2005

본 연구는 고등학교 가정과의 ${\ulcorner}$가정 생활의 설계${\lrcorner}$ 단원을 중심으로 실험수업을 실시하여 양성평등 수업이 고등학생의 남녀평등의식에 미치는 효과를 분석하여 양성평등의식 함양과 양성평등교육의 기초를 마련하고자 하였다. 그 결과를 요약하면 다음과 같다. 1. ${\ulcorner}$가정 생활의 설계${\lrcorner}$ 단원에서 양성평등 수업 후 남녀평등의식의 가정생활영역, 학교생활영역, 직업생활영역, 사회문화생활영역 모두에서 실험집단이 비교집단보다 남녀평등의식이 더 높게 나타나 두 집단간에 통계적으로 유의한 차이가 나타났다. 2. ${\ulcorner}$가정 생활의 설계${\lrcorner}$ 단원에서 양성평등 수업 전과 후 실험집단의 남녀평등의식 사후검사가 사전검사보다 높게 나타나 통계적으로 유의한 차이가 나타난 반면 비교집단에서는 사전검사와 사후검사 간에 통계적으로 유의한 차이가 나타나지 않았다. 3. ${\ulcorner}$가정 생활의 설계${\lrcorner}$ 단원에서 양성평등 수업 후 남녀평등의식 전체에서 실험집단의 변화량이 비교집단의 변화량보다 높게 나타나 통계적으로 유의한 차이가 나타났다. 이상의 연구 결과로 볼 때 고등학교 가정과의 ${\ulcorner}$가정 생활의 설계${\lrcorner}$ 단원에서 실험집단에 적용한 양성평등 수업은 가정생황영역, 학교생활영역, 직업생황영역, 사회문화생황영역 4개 영역 모두 고등학생의 남녀평등의식을 높이는데 효과적이었다. 무의식 속에 내재되어 있는 성차별 의식이 짧은 기간에도 불구하고 효과가 있는 것으로 볼 때, 앞으로 지속적인 지도가 절실히 필요하다.

• Journal of Microbiology and Biotechnology
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• 제20권2호
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• pp.370-374
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• 2010

A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.

• BMB Reports
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• 제41권4호
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• pp.294-299
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• 2008

Elevated expression of SMYD3 is a frequent genetic abnormality in several malignancies. Few studies knocking down SMYD3 expression in cervical carcinoma cells have been performed to date. In this paper, we established an inducible short hairpin RNA expression system to examine its role in maintaining the malignant phenotype of HeLa cells. After being induced by doxycycline, SMYD3 mRNA and protein expression were both reduced, and significant reductions in cell proliferation, colony formation and migration/invasion activity were observed in the SMYD3-silenced HeLa cells. The percentage of cells in sub-G1 was elevated and DNA ladder formation could be detected, indicating potent induction of apoptosis by SMYD3 knockdown. These findings imply that SMYD3 plays crucial roles in HeLa cell proliferation and migration/invasion, and that it may be a useful therapeutic target in human cervical carcinomas.

• 한국지역지리학회지
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• 제8권4호
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• pp.492-512
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• 2002

본 연구는 최근 다변화되고 있는 초등학생의 일상생활 활동의 시간배분 구조 및 주요 일상생활 활동의 시 공간적 특성을 고찰하였다. 일상생활 활동의 시간배분 구조는 학교 활동, 과외 활동 그리고 가내 학습활동을 중심으로 매우 단조로운 패턴을 가지며, 여가 활동을 비롯한 기타 활동은 이들 활동간의 틈새 시간을 활용하는 부수적인 기능에 그치고 있다. 등 하교 통행 활동은 최근 맞벌이 부부 및 승용차 보급률의 증가 그리고 과외 및 오락 활동을 위한 각종 기능 시설의 증가에 따라 다변화되고 있다. 사설 학원 중심의 과외 활동은 시간과 통행 자율성의 제한으로 인해 과외 시설의 선정에 있어서는 거주지와의 접근성이 중요한 요인이 되고 있다. 여가 활동은 실내 놀이가 중심이며, 문제점으로는 여가 시간의 절대적 부족과 더불어 이들 여가 활동의 시 공간적 성격은 부모들의 여가활동에 대한 제도적 환경에 의하여 영향을 받고 있다는 점을 들 수 있다.

• 기본간호학회지
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• 제24권3호
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• pp.209-218
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• 2017

Purpose: The purpose of this research was to identify factors that influence health-related quality of life of patients who have had knee or hip arthroplasty. Factors included sleep disorders, pain, activities of daily living, and depression. Methods: The participants in this research were 97 patients within one year after receiving knee or hip arthroplasty surgery and who regularly visited a hospital outpatient department. Data were analyzed using descriptive statistics, t-test, ANOVA, and stepwise regression analysis with the SPSS program. Results: Sleep disorders, pain, activities of daily living, and depression had significant correlations with health related quality of life. Factors influencing health-related quality of life among arthroplasty patients were the length of time since surgery, sleep disorders, activities of daily living, and depression. These factors explained 58% of the participants' health-related quality of life. Conclusion: The results show that various factors can affect patients' health-related quality of life following joint replacement surgery. The findings indicate that sleep disorders and depression should receive continued attention after the arthroplasty. It is also necessary to develop appropriate nursing intervention programs to lower depression and increase patient participation in daily living activities.

• 가정과삶의질연구
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• 제33권6호
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• pp.51-63
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• 2015

This study explored the effects of after-school services on whether they reduced the possibility of self-care of children. In terms of characteristics of the use of after-school services, it focused on the 1) types, 2) diversity, and 3) use patterns of the after-school services. For the analysis, it used data of the cohort of elementary-school children from the Korean Children and Youth Panel Survey. The main findings are as follows. First, private mentoring and after-school services provided by welfare centers reduced the possibility of self-care. Second, when the total amount of educational time was controlled, the diversity of participating after-school services did not explain the possibility of self-care. Third, when the total amount of educational time was controlled, the use patterns of after-school services did not explain the possibility of self-care. Based on these findings, it suggested policy implications and future research.