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http://dx.doi.org/10.4014/jmb.0906.06029

Cloning of aprE86-1 Gene Encoding a 27-kDa Mature Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1  

Lee, Ae-Ran (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Kim, Gyoung-Min (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Kwon, Gun-Hee (Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Kang-Wook (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Park, Jae-Yong (Institute of Agriculture and Life Science, Gyeongsang National University)
Chun, Ji-Yeon (Department of Food Science and Technology, Sunchon National University)
Cha, Jae-Ho (Department of Microbiology, Pusan National University)
Song, Young-Sun (School of Food and Life Science, Inje University)
Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.2, 2010 , pp. 370-374 More about this Journal
Abstract
A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.
Keywords
Bacillus amyloliquefaciens; fibrinolytic enzymes; gene expression;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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