• Title/Summary/Keyword: advanced characterization

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Characterization and Antioxidant Activity of the Exopolysaccharide Produced by Bacillus amyloliquefaciens GSBa-1

  • Zhao, Wen;Zhang, Jian;Jiang, Yun-Yun;Zhao, Xiao;Hao, Xiao-Na;Li, Liu;Yang, Zhen-Nai
    • Journal of Microbiology and Biotechnology
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    • v.28 no.8
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    • pp.1282-1292
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    • 2018
  • The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predominantly glucose, and it was further confirmed by NMR spectroscopy to be ${\alpha}-glucan$ that consisted of a trisaccharide repeating unit with possible presence of two ${\alpha}-(1{\rightarrow}3)$ and one ${\alpha}-(1{\rightarrow}6)$ glucosidic linkages. Microstructural analysis showed that the EPS appeared as ellipsoid or globose with a smooth surface. The EPS had a degradation temperature at $240^{\circ}C$. Furthermore, the EPS had strong DPPH and hydroxyl radical scavenging activities, and moderate superoxidant anion scavenging and metal ion-chelating activities. This is the first characterization of a glucan produced by B. amyloliquefaciens with strong antioxidant activity. The results of this study suggest the potential of the EPS from B. amyloliquefaciens GSBa-1 to serve as a natural antioxidant for application in functional products.

Modeling and Characterization of Low Voltage Access Network for Narrowband Powerline Communications

  • Masood, Bilal;Haider, Arsalan;Baig, Sobia
    • Journal of Electrical Engineering and Technology
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    • v.12 no.1
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    • pp.443-450
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    • 2017
  • Nowadays, Power Line Communication (PLC) is gaining high attention from industry and electric supply companies for the services like demand response, demand side management and Advanced Metering Infrastructure (AMI). The reliable services to consumers using PLC can be provided by utilizing an efficient PLC channel for which sophisticated channel modeling is very important. This paper presents characterization of a Low Voltage (LV) access network for Narrowband Power Line Communications (NB-PLC) using transmission line (TL) theory and a Simulink model. The TL theory analysis not only includes the constant parameters but frequency selectivity is also introduced in these parameters such as resistance, conductance and impedances. However, the proposed Simulink channel model offers an analysis and characterization of capacitive coupler, network impedance and channel transfer function for NB-PLC. Analysis of analytical and simulated results shows a close agreement of the channel transfer function. In the absence of a standardized NBPLC channel model, this research work can prove significant in improving the efficiency and accuracy of NB-PLC communication transceivers for Smart Grid communications.

Large-scale Genotyping and Genetic Mapping in Plasmodium Parasites

  • Su, Xin-Zhuan;Jiang, Hongying;Yi, Ming;Mu, Jianbing;Stephens, Robert M.
    • Parasites, Hosts and Diseases
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    • v.47 no.2
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    • pp.83-91
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    • 2009
  • The completion of many malaria parasite genomes provides great opportunities for genomewide characterization of gene expression and high-throughput genotyping. Substantial progress in malaria genomics and genotyping has been made recently, particularly the development of various microarray platforms for large-scale characterization of the Plasmodium falciparum genome. Microarray has been used for gene expression analysis, detection of single nucleotide polymorphism (SNP) and copy number variation (CNV), characterization of chromatin modifications, and other applications. Here we discuss some recent advances in genetic mapping and genomic studies of malaria parasites, focusing on the use of high-throughput arrays for the detection of SNP and CNV in the P. falciparum genome. Strategies for genetic mapping of malaria traits are also discussed.

Benchmarking of Zinc Coatings for Corrosion Protection: A Detailed Characterization of Corrosion and Electrochemical Properties of Zinc Coatings

  • Wijesinghe, Sudesh L;Zixi, Tan
    • Corrosion Science and Technology
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    • v.16 no.1
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    • pp.38-47
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    • 2017
  • Due to various types of Zn coatings for many decades for various applications, it is imperative to study and compare their corrosion resistance properties of some of these. Here, we introduce a systematic methodology for evaluation and validation of corrosion protection properties of metallic coatings. According to this methodology, samples are were exposed in an advanced cyclic corrosion test chamber according to ISO 14993, and removed at the end of each withdrawal for respective corrosion and electrochemical characterization to evaluate both barrier and galvanic protection properties. Corrosion protection properties of coatings were evaluated by visual examination according to ISO 10289, mass loss and subsequent corrosion rate measurements, electrochemical properties, and advanced electrochemical scanning techniques. In this study, corrosion protection properties of a commercial zinc rich coating (ZRC) on AISI 1020 mild steel substrates were evaluated and benchmarked against hot dip galvanized (HDG). Results were correlated, and corrosion protection capabilities of the two coatings were compared. The zinc rich coating performed better than hot dip galvanized coating in terms of overall corrosion protection properties, according to the exposure and experimental conditions used in this study. It proved to be a suitable candidate to replace hot dip galvanized coatings for desired applications.

Microstructure and Characterization of Overlay Welding Layer using Fe-based Composite Powders (철계 복합 분말로 제조된 오버레이 용접층의 미세조직 및 특성)

  • Min, Hong;Lee, Jong-Jae;Lee, Jin Kyu
    • Journal of Powder Materials
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    • v.26 no.3
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    • pp.214-219
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    • 2019
  • In this study, the microstructure and characterization of an overlay welding layer using Fe-based composite powders are reported. The effects of the number of passes and composition of powders on the microstructure and mechanical properties are investigated in detail. The welding wire and powders are deposited twice on a stainless-steel rod using a laser overlay welding process. The microstructure and structural characterization are performed by scanning electron microscopy and X-ray diffraction. The mechanical properties of the first and second overlay layers are analyzed through the micro-Vickers-hardness tester and abrasion wear tester. In the second overlay layer, the hardness and specific wear are approximately 840 Hv and $2.0{\times}10^{-5}mm^3/Nm$, respectively. It is suggested that the increase of the volume fractions of $(Cr,Fe)_7C_3$ and NbC phases in the second welding layer enhances the hardness and wear resistance.

Statistical Methodologies for Scaling Factor Implementation: Part 1. Overview of Current Scaling Factor Method for Radioactive Waste Characterization

  • Kim, Tae-Hyeong;Park, Junghwan;Lee, Jeongmook;Kim, Junhyuck;Kim, Jong-Yun;Lim, Sang Ho
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.4
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    • pp.517-536
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    • 2020
  • The radionuclide inventory in radioactive waste from nuclear power plants should be determined to secure the safety of final repositories. As an alternative to time-consuming, labor-intensive, and destructive radiochemical analysis, the indirect scaling factor (SF) method has been used to determine the concentrations of difficult-to-measure radionuclides. Despite its long history, the original SF methodology remains almost unchanged and now needs to be improved for advanced SF implementation. Intense public attention and interest have been strongly directed to the reliability of the procedures and data regarding repository safety since the first operation of the low- and intermediate-level radioactive waste disposal facility in Gyeongju, Korea. In this review, statistical methodologies for SF implementation are described and evaluated to achieve reasonable and advanced decision-making. The first part of this review begins with an overview of the current status of the scaling factor method and global experiences, including some specific statistical issues associated with SF implementation. In addition, this review aims to extend the applicability of SF to the characterization of large quantities of waste from the decommissioning of nuclear facilities.

Establishment of ICT Specialized Teaching-Learning System in the Era of Superintelligence, Super-Connectivity, and Super-Convergence

  • Seung-Woo LEE;Sangwon LEE
    • International journal of advanced smart convergence
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    • v.12 no.3
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    • pp.149-156
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    • 2023
  • Joint research on software, electronic engineering, computer engineering, and financial engineering and the use of ICT knowledge through network formation play an important role in strengthening science and technology-based innovation capabilities and facilitating the development and production process of products using new technologies. For the purpose of this study, I would like to strategically propose ICT specialized education in the 4th industrial revolution. To this end, the ICT specialization model, ICT specialization strategy analysis, and ICT specialization operation and effect were explored to establish ICT specialization strategies centered on software, electronic engineering, computer engineering, and financial engineering in the era of super-intelligence, hyper-connected, and hyper-convergence. Secondly, a roadmap for detailed promotion tasks related to efficient ICT characterization based on core strategies, detailed promotion tasks, and programs was proposed, focusing on talent related to ICT characterization. Thirdly, we would like to propose a reorganization of the academic structure and organization related to ICT characterization. Finally, we would like to propose the establishment of a future-oriented education system related to ICT specialization based on the advanced education and research environment.

Synthesis and Characterization of ABA Type Block Copolymers of Trimethylene Carbonate and $\varepsilon$-caprolactone (Trimethylene Carbonate 와 $\varepsilon$-caprolactone ABA 트리블럭 공중합체의 합성 및 특성)

  • Jia, Yong-Tang;Kim, Hak-Yong;Jian Gong;Lee, Duok-Rae;Bin Ding;Narayan Bhattarai
    • Proceedings of the Korean Fiber Society Conference
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    • 2002.04a
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    • pp.352-354
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    • 2002
  • A series of ABA type triblock copolymers of trimethylene carbonate (TMC) and $\varepsilon$-caprolactone($\varepsilon$-CL) with different molar ratio were synthesized using ethylene glycol as initiator and stannous octoate as catalyst by ring-opening bulk polymerization. The characterization of the triblock copolymers was characterized by $^1$H-NMR, $\^$13/C-NMR, FT-IR, GPC and DSC, and compared with random copolymer. (omitted)

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Cloning and characterization of the psbA Gene from Panax ginseng(Characterization of the psbA Gene from P. ginseng)

  • Lee, Won-Kyu;Tae, Gun-Sik
    • Journal of Photoscience
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    • v.10 no.3
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    • pp.245-249
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    • 2003
  • The psbA gene of photo system II was cloned and characterized from the P. ginseng chloroplast. The psbA gene is composed of 1,062 nucleotides. The overall amino acid sequence shows 99% and 98% identities to dicots and monocots of higher plants, respectively. Southern blot analysis revealed that a single copy of the psbA gene existed in the chloroplast genome. Northern blot analysis of the in vivo accumulation of the psbA transcript, after being grown under the different intensities (5%, 10%, 20%, and 100%) of daylight, indicated that the steady-state level of the psbA transcript was not significantly affected by light intensity.

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Characterization of ultra Precision Grinding Plate for GMR Head Manufacturing by Measuring Frictional Force (마찰력 측정을 통한 GMR 헤드 제작용 초정밀 연마판의 특성화)

  • 노병국;김기대
    • Journal of the Korean Society for Precision Engineering
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    • v.20 no.7
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    • pp.78-83
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    • 2003
  • Characterization of an ultra precision grinding plate for GMR head manufacturing is performed by measuring frictional forces between the grinding plate and the advanced ceramic Two kinds of methods of producing the precision grinding plates are presented: texturing and micro-channeling. Texturing is effective in terms of production time but micro-channeling excels in quality control. It is found that the frictional coefficient of a precision grinding plate decreases as the impregnation of diamond grain onto the precision-grinding plate progresses, and remains unchanged once the impregnation process is successfully completed, even after 100 revolutions of the precision-grinding plate against the advanced ceramic under 40 N of normal force. Therefore, the measurement of the frictional coefficient can replace costly and time-consuming process of estimating the level of impregnation of diamond grain on the precision-grinding plate, which has been performed by using scanning electron microscope, and be employed as an index to determine the level of impregnation of diamond grain.