• Toxicological Research
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• 제21권1호
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• pp.63-70
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• 2005

Gami-Honghwa-Tang (KH-19), a herbal prescription for reducing the side effect of radiotherapy, is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos, and Glycyrrhizae Radix. In this study, marker substances in KH-19 were analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD) and safety evaluation of standardized KH-19 was evaluated by good laboratory practices (GLP) guideline of Korea Food and Drug Administration. HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. In acute oral toxicity study on rat, transient inhibition of body weight was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and body weight reduction were observed, However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

• 생약학회지
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• 제24권2호
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• pp.166-176
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• 1993

This study was attempted to investigate the acute toxicity in mice, the subacute toxicity in rats of Cynanchi wilfordii Radix extract, the effect on transaminase activities, hematological parameters, leukocyte parameters in serum of subacute-toxicated rats. In acute toxicity test, the death rate was not observed in 50, 100, 200, 300 mg/kg(i.p.), one tenths to two tenths in 300, 500, 1000, 1500 mg/kg(p.o.) for two weeks. In subacute test, rats were all died in 300 mg/kg(p.o.) during 4 weeks, in 500, 1000 mg/kg(p.o.) during three weeks. The cause of death believed to be stomach ulcer. The activities of S-GOT and S-GPT were significantly increased in all sample-treated groups, when compared with the normal groups. A number of WBC and neutrophil belong to hematological parameter were significantly increased, lymphocyte was decreased in all sample-treated group, when compared with normal group. The hemolytic action on water extract, saponin and alcohol extract showed very low activities.

• 대한본초학회지
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• 제23권2호
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• pp.81-85
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• 2008

Objectives : The aim of this study is to collect data for toxicity and safety of Mud chrysanthemum indicum. Methods : In this study, we investigated the acute toxicity for buthanol-extracted Mud schrysanthemum indicum, 25 male and 25 female mice were observed for 14days after one day oral administration of Mud chrysanthemum indicum at the respective doses of 0(control group), 1024, 1280, 1600 and 2000mg/kg. Results : We observed survival rates, general toxicity, change of body weight and autopsy. No abnormality was found for all cases. Conclusions : The data confirmed that Mud chrysanthemum indicum is free from, the toxicity and safety problems. Compared with the control group, we could not find any toxic alteration in all treated groups (1024, 1280, 1600 and 2000mg/kg), In conclusion, LD50 of Mud chrysanthemum indicum was over 2000 mg/kg and it is very safe to mice.

• 한국안전학회지
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• 제30권4호
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• pp.51-55
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• 2015

There are various studies on the fire suppression process but the study on second pollution from fire products is not enough yet. Therefor, in this study verify that environmentally-friendly properties($LC_{50}$) of foam extinguishing agent with increases its amount used through with Fish-Acute Toxicity Test using a fish named Misgurnus anguillicaudatus that is appointed by OECD Test Guideline. In conclusion, proven that environmentally friendly properties of the agent of hoseo university through 16 times of LC50 than that of market.

• 생약학회지
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• 제39권4호
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• pp.352-356
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• 2008

Acute toxicity of standardized extract of Salvia miltiorrhiza Bunge was examined using male and female ICR mice. Mice were treated with standardized extract the intragastrically at 5 mg/kg, 50 mg/kg, 500 mg/kg or 2,000 mg/kg and observed for two weeks. At the doses used, no mortality or abnormal clinical signs in animals were shown during at the observation period. Also there was no difference in net body weight gain, gross pathological findings at the terminal sacrifice among the groups mice treated with different doses of the test substance. The results suggested that acute oral toxicity of standardized extract of S. miltiorrhiza in mice is very low at the conditions employed in this study.

• Biomolecules & Therapeutics
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• 제10권2호
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• pp.114-116
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• 2002

This study was performed to investigate the acute toxicity of PEG-hemoglobin SB1, a blood substitute, in beagle dogs. The male and female dogs were administered intravenously at the doses of 0.4375, 0.875 and 1.75 g/kg body weight, respectively. After a single intravenous administration of SB1 to dogs, we observed them daily for 2 weeks. SB1 did not induce any toxic signs in the mortalities, clinical signs, body weight changes, and gross necropsy findings of dogs. Based on these results, acute toxicity, dogs SB1 may have no side effect and its $LD_{50}$ value may be over 1.75 g/kg (25 ml/kg) of body weight in dogs.

• 한국한의학연구원논문집
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• 제13권1호통권19호
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• pp.161-164
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• 2007

SsangHwaTang has been traditionally prescribed a medicine as a restorative. In this study, We investigated the acute toxicity about water-extracted SsangHwaTang. Twenty-five mice completed 14 days of oral SsangHwaTang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000mg/kg. And then we observed survival rates, general toxicity, change of body weight, and autopsy. Compared with the control group, we could not find any toxic alteration in all treated groups (2560, 3200, 4000 and 5000mg/kg). In conclusion, LD50 of SsangHwaTang was over 5000mg/kg and it is very safe to ICR mice.

• 생명과학회지
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• 제11권2호
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• pp.97-102
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• 2001

This study was performed to evaluate the actue sbucutaneous toxicity of hydroxyapatite sinter produced from tuna bone in Sprague-Dawley(SD) rats. Hydroxyapatite sinter was administrated at dose levels of 5000, 2500, 1250, 625, 312.5 and 0 mg/kg. After single subcutaneous adiminstration to both sexes to both sexes SD rats, we observed rats for 14 days. Hydroxyapatite sinter did not induce any toxic signs inmortalities, clinical findings, body weights and gross findings of the rats. In view of result, it was impossible to estimate LD/ sub 50/ values in SD rats. In conclusion, these results suggest that hydroxyapatite sinter produced from tuna bone has no effect on acute subcutaneous toxicity in SD rats.

• 대한예방한의학회지
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• 제10권1호
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• pp.123-140
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• 2006

This experiment was carried out to study on the safety assessment of Pinus densiflora Siebold et Zuccarini for Hebal-acupuncture. SD rats and ICR mice were used for acute toxicity test, the results were summerized as follows; 1. In rats and mice, $LD_{50}$ value could not be measured. 2. There were no abnormal finding in acute toxicity test treated Pinus densiflora Siebold et Zuccarini for Hebal-acupuncture.

• Toxicological Research
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• 제13권4호
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• pp.349-352
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• 1997

Scopoliae rhizoma is a perennial herb which has a similar effect with atropine on the cardiovascular system. It is also known to have a seditive and anticonvulsant activity on the central nerve system. In order to evaluate an acute toxicity of Scopoliae rhizoma, the present study was performed after administration the Scopoliae rhizoma prepared by both decoctional and frozen dried extract through three different routes (oral; 5,000 mg/kg, intraperitoneal; 2,000 mg/kg, subcutaneous; 5,000 mg/kg) to the female ICR mice. In the group treated intraperitoneally with a frozen dried extract, abnormal clinical signs such as decreased activity, crouch, potosis and abnormal walking were observed for 40 rain after administration. With regard to WBC, decreased number of lymphocyte and increased number of monocyte and granulocyte were also observed in the animals received intraperitoneally with Scopoliae rhizoma extract. Taken together, what toxicity of Scopoliae rhizoma was shown differently depending on its type for administration may be resulted in the differency of administered dose. The results provided here support a pharmacological and toxicological consideration for its clinical use in the regard of oriental medicine.