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Statistical Optimization of Culture Conditions of Probiotic Lactobacillus brevis SBB07 for Enhanced Cell Growth (프로바이오틱 Lactobacillus brevis SBB07의 균체량 증가를 위한 배양 조건 최적화)

  • Jeong, Su-Ji;Yang, Hee-Jong;Ryu, Myeong Seon;Seo, Ji Won;Jeong, Seong-Yeop;Jeong, Do-Youn
    • Journal of Life Science
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    • v.28 no.5
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    • pp.577-586
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    • 2018
  • We recently reported the potential probiotic properties of Lactobacillus brevis SBB07 isolated from blueberries. The present study investigates the effect of culture conditions such as temperature, initial pH, culture time, and medium constituent for industrial application. The ingredients of the medium to improve cell growth were selected by Plackett-Burman design (PBD) and central composite design (CCD) within a desirable range. The PBD was applied with 19 factors: seven carbon sources, six nitrogen sources, and six microelements. Protease peptone, corn steep powder (CSP), and yeast extract were found to be significant factors for the growth of SBB07. The CCD was then applied with three variables found from the PBD at five levels, and the optimum values were decided for the three variables: protease peptone, CSP, and yeast extract. In the case of the growth of SBB07, the proposed optimal media contained 2.0% protease peptone, 2.5% CSP, and 2.0% yeast extract, and the maximum dried-cell weight was predicted to be 2.93963 g/l. By the model verification, it was confirmed that the predicted and actual results are similar. Finally, the study investigated the effects of incubation temperature and initial pH at the optimized medium. It was confirmed that the dried-cell weight increased from $2.2933{\pm}0.0601g/l$ to $3.85{\pm}0.0265g/l$ when compared to the basal medium at $37^{\circ}C$ and initial pH 8.0. Establishing the optimal culture condition for SBB07 provides good potential for applications in probiotics and can serve as the foundation for the industrialization of materials.

A Study on Martial art for suggesting the role of Martial art Sports as a Leisure Activity (여가활동으로서의 무도스포츠 역할 재고를 위한 고찰)

  • Lim, Young-Sam
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.3
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    • pp.564-570
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    • 2020
  • The purpose of this study is to examine the preceding studies and suggest alternatives to establish the role and value of martial arts sports as a leisure activity. To achieve the purpose of this study, the keywords and themes of leisure-related journals were extracted and the articles and current status of martial arts-related journals in Korea were derived using SPSS descriptive statistics method. The subjects of the analysis were 'mudo' and 'leisure' of leisure-related journals between 2005 and 2017, and an interpretative textual analysis was conducted to analyze the contents of individual studies. According to the results of the survey on the actual condition of participation in the 2016 National Sports for all, Taekwondo was ranked 5th among the top 5 sports with 6.1% of the sports for all, and Taekwondo and Kendo were ranked 1st and 2nd respectively in the sports for all students and the clubs that they want to join in the future. Second, the study on martial arts in the journals related to leisure was found most in 2006 and 2010, but only one study was not conducted after 2014, which confirmed that the absolute number of studies was very insufficient. Third, the research themes of the journals related to leisure were serious leisure, female college students, physical self-concept, social development, leisure recreation class, job satisfaction, life satisfaction, training, leisure constraints, etc., and the study of martial arts related to leisure was found to require quantitative and qualitative multilateral approaches. Fourth, in the current status of dance related studies by year in domestic journals, two of 23 studies in 2007 were conducted on leisure topics, and the average number of studies related to domestic martial arts and leisure related papers was 5.65%, which is very low. In conclusion, as a result of analyzing the trend of research on leisure as a martial arts sport, it is necessary to suggest the direction of future research that can reconsider the role and value of martial arts sport as a leisure activity that can improve the quality of life and happiness in future society through quantitative and qualitative improvement.

A Survey on the Forest Cultural Assets in the Southern Coastal Areas for the Designation and Maintenance of Law-protected Trees (보호수 지정 및 관리를 위한 남해안 지역 산림문화자산 실태조사)

  • Kang, Kee-Rae
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.30 no.2
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    • pp.28-36
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    • 2012
  • Forests are one of the basic assets nearest our daily lives that provide the foundation of life necessities, culture and even survival. These assets pervade local residents' lives as culture and the necessities of life, and they will be handed down to posterity. Therefore, this study was conducted to carry out a field survey on old trees, plant communities and forests in the southern coastal areas in Korea, where not many surveys were not conducted on the forest cultural assets, further examining a possibility that some of the trees should be designated as law-protected. As for the survey of plants, this study investigated 80 different spots of old trees, plant communities and forests, located in 11 cities and counties in Gyengnam Province and Jeonnam Province adjacent to the southern coast of Korea. To measure the health and actual maintenance conditions of old trees and plant communities objectively, the standard of judgment was established through the brain storming of experts. As a result of the field survey, out of all the old trees and plant communities that are not law-protected, this study selected old trees and plant communities that are in good health and likely to pass the standard of designating law-protected trees according to the Established Rule 574 of the Korea Forest Service Regulation. As for the plant community and old trees likely to be law-protected, this study judged that the torreya forest in Naneum Li, Namhae and old trees in 13 spots, in cluding so-called 'Couple Pine Tree' in Pyeongsa Li, Hadong, have a high possibility of being designated as a law-protected plant community and old trees. As for the species of old trees, there were 3 zelkova serrata trees, 3 celtis sinensis persoon trees, 2 pinus densiflora trees, 1 castanopsis cuspidata tree, 1 salix chaenomeloides tree and 1 machilus thunbergii tree found in the survey areas. Overall, this study judged that those old trees and plant communities require a national systematic maintenance by being designated as law-protected ones, and their values should be handed down to posterity as our forest cultural assets.

Status of Meal Serving and Nutritional Quality of Foods Served for Children at Community Child Centers in Korea (지역아동센터의 배식 실태 및 배식량 측정을 통한 영양적 질 평가)

  • Kwon, Sooyoun;Yeoh, Yoonjae
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.2
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    • pp.352-362
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    • 2015
  • The purpose of this study was to examine portion sizes and nutritional quality of foods served to children at Community Child Centers (CCCs), July 2014, in Korea. A survey was administered to foodservice employees working at CCCs. Thirty-nine participants completed the self-administered questionnaire regarding CCCs foodservice practices. In the weight test, fifteen volunteers of demonstrated typical portion sizes for $5^{th}$ grade elementary school students. Nutrition knowledge scores were significantly different between foodservice employees with experience taking a foodservice class for one year (6.04 out of 10.0 points) and those without experience (4.58 points). Foodservice employees with experience taking a foodservice class scored significantly higher in performing meal serving practices, e.g., wearing a sanitary cap and apron when serving food, than those without experience. The amount of foods served for children did not meet standard serving sizes; portion sizes of rice, soup, main dish, side dish and kimchi served by foodservice employees were 87.3%, 63.2%, 56.5%, 37.1% and 81.3% of standard serving sizes, respectively. When energy and nutrient intakes from portion sizes were calculated, energy, vitamin A, thiamin, riboflavin and calcium intakes did not meet standards for nutrition control of school meals. However, protein, vitamin C and iron intakes met more than 100.0% of standards. These findings suggest that foodservice employees with experience taking a foodservice class apply their knowledge to foodservice practices. Although portion sizes can influence energy and nutrient intakes in children, the actual portion sizes served by CCCs foodservice employees were inadequate and did not meet standards for serving size and nutrition. Thus, all CCCs foodservice employees need to receive foodservice education and be provided guidelines regarding portion sizes for children.

A Study on Nurse' Image in a Medical Center (일 대학병원 간호사 이미지에 관한 연구)

  • Han, Sang-Sook;Sohn, In-Soon;Lee, Myung-Hai;Choi, Kyoung-Soon
    • Journal of East-West Nursing Research
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    • v.8 no.1
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    • pp.113-125
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    • 2003
  • This study is a descriptive investigation into the image of nurses, and attempted to help to advance the profession of nursing and to provide basic data for developing strategies to improve the image of nurses. The Subject of this study was a group of 380 persons from a K Medical Centre in Seoul, including the hospital patients and their guardians, as well as the doctors, assistants and hospital administrative staff. The data have been collected from the 10th to the 30th of May, 2003. We have developed a research tool of 40 questions divided into three categories using a tool developed by Kim, H.J and KIm, H.O.(2001) verifying its construct validity. The reliability of the tool was Cronbache's ${\alpha}=.97$, and by categories, Cronbach's ${\alpha}=.86$ for service image, Cronbach's ${\alpha}=.96$ for professional image and Cronbache's ${\alpha}=.90$ for social image. The collected data have been analysed according to the purpose of this study using SPSS WIN 11.0 for real number, percentage, factors analysis, multiple regression analysis, ANOVA and $x^2$-test, and the results are as follows: 1) There was a significant difference in the image of nurses by job series of the subjects; from patients and guardians for 4.01 to doctors 3.62, assistants 3.54 and staff members 3.41 (F=36.14, p=.000). As well, there was a significant difference in service, professional and social image categories according to the position of the subjects ($F=20.36{\sim}42.35$, p=.000). 2) The main factors that affect on formation the nurse's imaging came by direct experiences with nurses at hospitals for 81.3%, by looking at the every life of the nurses that the subjects personally know for 15.5%, by mass media for 1.6% and by the accounts from the others for 1.6%. 3) 78.4% of the subjects considered that the image of nurses on mass media is described better than for real, 8.2% believed that the image is described worse than for real, and only 13.2% of the subjects perceived that the image of nurses on mass media corresponds the image of nurses in actual life. 4) 74.5% of the subjects said that they got a better image of nurses after their hospitalization while 2% got a worse one and 23.5% said to have had no changes, and the period of hospitalization had no relevance to the image of nurses (X2=5.04, P=.489). However, while 16.8% of the subjects who spent less than one week in hospital said that they got a better image of nurses, 27.5% of those who spent longer than four weeks got a better image of nurses. 5) There was a significant difference in the total image points of nurses by the patients and their guardians according to the period of hospitalization; 4.14 for 1 to 2 weeks, 4.07 for 2 to 4 weeks, 4.02 for 4 weeks and longer and 3.80 for less than a week (F=3.40, P=.019). Upon the results stated above, I should like to propose as below: 1) An investigative enquiry is needed to improve the image of nurses as though being a nurse is very hard and difficult. 2) A continuous monitoring in mass media is needed to create a positive image of nurses.

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Students' Experience and Preference on Student Activities in the Clothing & Textiles Section of Middle School 'Technology.Home Economics' Textbooks (중학교 기술.가정 교과서 의생활 영역 옷차림 단원의 활동과제에 대한 학습자의 수행경험과 선호도 조사 연구)

  • Eo, Ji-Hyun;Oh, Kyung-Wha
    • Journal of Korean Home Economics Education Association
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    • v.21 no.1
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    • pp.51-69
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    • 2009
  • This study is intended to provide fundamental information to improve the quality of student activities presented in the Clothing & Textiles How to Dress Appropriately' section of the current middle school 'Technology Home Economics' textbooks so that Home Economics may better reflect students' interests, making it applicable in real life. The survey was conducted to 154 male and 160 female students on their preferences regarding student activities. The results are as follows. First, students who like clothing & textiles section regard "Opportunities to take part in various kinds of practices and student activities" as the major reason for preference. And the single biggest reason why they dislike the unit was due to "Too much contents to be memorized." Among various contents regarding dress in the unit, "How to Wear Clothes That Look Good on Me, and the Right Ways to Wear Them" attracted the most attention, regardless of what contents they consider necessary, interesting, or helpful in real life. Second, as for the time of implementation of the activities, students preferred "End of each class". They also preferred small-group activities (group size), well-structured problems (type of problems) and tasks that require analysis based on theoretical principles through experiments and practices (methods of implementation). Third, the findings as to the actual experience of conducting the student activities indicated that, in most cases, student activities were conducted in accordance to what was suggested in the textbooks, but not to what the students preferred. Therefore, in order to make home economics more applicable to students, it is desirable to focus on their everyday lives as is favored by the students, and increase small-group activities. Also, suggesting various and comprehensive problematic situations such as non-structured, open-ended problems and encouraging diverse implementation would be helpful in improving students' critical and creative thinking abilities.

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A Case study and Analysis on the Up-Lift Pressure Treatment Evaluation of Underground Installations for their Efficient Adoption (사례분석을 통한 효율적 상향수압(Up-Lift Pressure) 처리공법 적용방안에 관한연구 - ◯◯ 상업지역 현장사례 중심으로 -)

  • Ko, Ok-Yeol;Kwon, Oh-Chul;Shim, Jae-Kwang;Park, Tae-Eun
    • Journal of the Korea Institute of Building Construction
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    • v.9 no.4
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    • pp.119-129
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    • 2009
  • Building construction trends have been changed dramatically in terms of size and mass. With the need to maximize land usage, there has been an increase in the construction of high-rise buildings. This affects not only the entire construction duration and cost, but also subsequent construction activities, such as work to increase underground facilities and in reclamation land area construction. These types of site conditions require soft ground reinforcement and the proper uplift water pressure treatment. In general, two kinds of methods have been used for uplift water pressure treatment systems. However, there have been some problems arising as the result of a lack of research and analysis on underground construction techniques, and a reliance on experiments over actual survey and analysis of site conditions. This paper focused on the problems of conventional selection procedure, by analyzing drawings and proposing a kind of modeling for a reasonable procedure. The results were applied to OO project as a sample construction case to be verified in this research. The initial plan in the case project was the Rock Anchor System. However, as there were terrible miscalculations of basic site conditions that had an extraordinary influence on the underground water level, such as the site's proximity to the Han-river, it was necessary to change the plan to include apermanent drainage system. This achieved a direct construction cost reduction \ 406,702,000 and a maximum sayings of 4% of operational cost, based on the 50-year building Life Cycle Cost.

Analysis of Environmental Design Data for Growing Pleurotus ervngii (큰 느타리버섯 재배사의 환경설계용 자료 분석)

  • Yoon, Yong-Cheol;Suh, Won-Myung;Lee, In-Bok
    • Journal of Bio-Environment Control
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    • v.14 no.2
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    • pp.95-105
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    • 2005
  • This study was carried out to file up using effect and requirement of energy for environmental design data of Pleurotus eryngii growing houses. Heating and cooling Degree-Hour (D-H) were calculated and compared for. some Pleurotus eryngii growing houses of sandwich-panel (permanent) o. arch-roofed(simple) type structures modified and suggested through field survey and analysis. Also thermal resistance (R-value) was calculated for the heat insulating and covering materials of the permanent and simple-type, which were made of polyurethane or polystyrene panel and $7\~8$ layers heat conservation cover wall. The variations of heating and cooling D-H simulated for Jinju area was nearly linearly proportional to the setting inside temperatures. The variations of cooling D-H was much more sensitive than those of heating D-H. Therefore, it was expected that the variations of required energy in accordance with setting temperature or actual temperature maintained inside of the cultivation house could be estimated and also the estimated results of heating and cooling D-H could be effectively used far the verification of environmental simulation as well as for the calculation of required energy amounts. When the cultivation floor areas are all equal, panel type houses to be constructed by various combinations of materials were found to by far more effective than simple type pipe house in the aspect of energy conservation maintenance except some additional cost invested initially. And also the energy effectiveness of multi-span house compared to single span together with the prediction of energy requirement depending on the level insulated for the wall and roof area could be estimated. Additionally, structural as well as environmental optimizations are expected to be possible by calculating periodical and/or seasonal energy requirements for those various combinations of insulation level and different climate conditions, etc.

Operator exposure risk assessment of benzimidazole fungicides on Korean agricultural condition (Benzimidazole계 살균제의 농작업자 위해성평가)

  • Lee, Je-Bong;Shin, Jin-Sup;Jeong, Mi-Hye;Park, Yeon-Ki;Im, Geon-Jae;Kang, Kyu-Young
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.347-353
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    • 2005
  • Pesticide risk assessment for pesticide operators as well as for consumers has become one of the pesticide regulatory tools to reduce any unreasonable adverse health effects from pesticide use. The risk for pesticide operators can be quantified by comparing the acceptable operator exposure level(AOEL) with exposure level during pesticide application. This study is to evaluate the risk of benzimidazole fungicides application worker. The exposure level of pesticide applicators were calculated using Japanese operator exposure study tested with EPN 45% EC. The AOELs for pesticides were obtained dividing relevant lowest no observed abuse effect levels(NOAELs) for the exposure scenario into uncertainty factor, 100. For the non-cancer and cancer occupational risk assessment, $Q_1^*$ produced by US/EPA and life time average daily dose(LADD) calculated from average daily dose(ADD), treatment days per year, worked years for life time were used. Operator exposure for benzimidazole fungicides application were benomyl 0.2, carbendazim 0.36 and thiophanate-methyl 0.42 mg/kg/day. Short-term AOELs for benomyl, carbendazim and thiophanate-methyl were 0.3, 0.1, and 0.2 mg/kg/day, and long-term AOEL were 0.025, 0.025, 0.08 mg/kg/day, respectively. LADDs were benomyl 0.0038, carbendazim 0.0067, thiophanate-methyl 0.0081 mg/kg/day. The ratios of exposure to AOEL were $0.28{\sim}1.5$ for short-term and $3.73{\sim}9.88$ for long-term. Cancer risk for operator were $9.12{\times}10^{-6}$ for benomyl, $1.61{\times}10^{-5}$ for carbendazim and $1.13{\times}10^{-4}$ for thiophanate-methyl by the standard application scenario. The result showed 3 fungicides exceed the risk criteria, $1.0{\times}10^{-6}$. The above risk assessments were based upon conservative assumptions and therefore are believed to be protective of the applicator. To refine the risk at the more actual conditions, further risk assessment with more realistic data would be needed.

Development and Application of Practical Problem-based Teaching·Learning Process for Interacting with Neighbors (이웃과 더불어 살아가는 주생활을 위한 실천적 문제 중심 교수·학습 과정안 개발 및 적용)

  • Woo, Yeseul;Cho, Jaesoon
    • Journal of Korean Home Economics Education Association
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    • v.30 no.3
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    • pp.67-90
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    • 2018
  • The purpose of this study was to develop and apply the practical problem-based teaching·learning process plan for 'interacting with neighbors' of home economics subject. The plan consisting of 3 lessons has been developed and implemented according to the ADDIE model. Various activity materials (7 student's activity sheets, 3 reading texts, 1 homework sheet, 3 sets of ppt, 6 videos, and 3 teacher's reading texts) as well as questionnaire were developed for the 3-session lessons. The plans were implemented by the researcher to 204 freshmen, 8 classes, of C middle school in Seoul during september, 2017. The result, of students' lower level of actual participation in interacting with neighbors comparing to their interests in, supported the need of this study. Students were satisfied with the whole 3-lessons in the aspects such as beneficial usage of the contents in their daily life and in building the sense of community, as well as adequacy of materials and activities. Students also reported that they would highly aware to the importance of interacting with neighbors and to practice the contents learned from the lessons in daily life at community. They had an opportunity to reflect one's own attitude to neighbors and recommended to teach it to other schools, too. It can be concluded that the teaching·learning process plan for 'interacting with neighbors' would raise students' housing values living together and attain the overall objective and achievement standards of 2015 home economics middle school curriculum.