• Title/Summary/Keyword: acp

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Arsenic-Induced Differentially Expressed Genes Identified in Medicago sativa L. roots

  • Rahman, Md. Atikur;Lee, Sang-Hoon;Kim, Ki-Yong;Park, Hyung Soo;Hwang, Tae Young;Choi, Gi Jun;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.3
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    • pp.243-247
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    • 2016
  • Arsenic (As) is a toxic element that easily taken up by plants root. Several toxic forms of As disrupt plant metabolism by a series of cellular alterations. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in alfalfa roots in response to As stress. Two-week-old alfalfa seedlings were exposed to As treatment for 6 hours. DEGs were screened from As treated samples using the ACP-based technique. A total of six DEGs including heat shock protein, HSP 23, plastocyanin-like domain protein162, thioredoxin H-type 1 protein, protein MKS1, and NAD(P)H dehydrogenase B2 were identified in alfalfa roots under As stress. These genes have putative functions in abiotic stress homeostasis, antioxidant activity, and plant defense. These identified genes would be useful to increase As tolerance in alfalfa plants.

Thermal Conductivity of Carbon-Phenolic 8-Harness Satin Weave Composite (탄소/페놀릭 8-매 주자직 복합재료의 열전도도)

  • Woo, Kyeong-Sik;Goo, Nam-Seo
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.31 no.9
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    • pp.18-25
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    • 2003
  • In this paper, thermal conductivities of carbon-phenolic 8-harness satin weave composite, ACP302, were measured and predicted. In the analysis, the satin weave unit cell was identified and modeled discretely by 3-dimensional finite elements, considering the interlaced fiber tow architecture microscopically. At the unit cell boundary, the corresponding periodic boundary conditions were applied. The results were analyzed to investigate the effect of microstructural parameters such as stacking phase shifts, waviness ratio, and fiber volume fraction. The conductivities were also obtained by experiments and compared with the numerical results.

Slitting Test of Simulated Fuel Rod by Using a Newly Developed Decladding Device (실증용 탈피복 장치를 이용한 모의 핵연료 슬릿팅 시험)

  • Jung, J.H.;Hong, D.H.;Kim, Y.H.;Park, B.S.;Lee, J.K.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2006.05a
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    • pp.141-144
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    • 2006
  • In this study, we developed a decladding device which separates 250 mm length of simulated nuclear spent fuel rod into the pallets and the pieces of the hulls after inserting the rod cut into the module with several pairs of blades. To improve the performance of the equipment, we considered some mechanisms to prevent the rod cut from being exposed or bounced into the hot-cell, to reduce the operation time, and to insert the rods automatically. It is expected that the newly developed system will contribute to prevent radioactive pollution in the hot-cell, reduce the operation time, and to increase the safety of the operators. As a result of the performance test for some mockup fuel rod cuts in the ACP(Advanced Spent Fuel Control Process) facility, it was verified that the decladding device could be applied to the actual fuel rod cut. And it will be able to use for a scale-up facility in the future.

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Application of Composites Composed of Phosphoric Acid-Doped Silica Gel and Styrene-Ethylene-Butylene-Styrene Elastomer to Electric Double-Layer Capacitors

  • Matsuda, Atsunori;Honjo, Hiroshi;Hirata, Kazuki;Tatsumisago, Masahiro;Minami, Tsutomu
    • The Korean Journal of Ceramics
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    • v.5 no.4
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    • pp.353-356
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    • 1999
  • Highly proten-conductive elastic composites have been successfully prepared from $H_3PO_4$-doped silica gel and styrene-ethylene-butylene-styrene block elastic copolymer. In addition solid state electric double-layer capacitors have been fabricated using the composite as an electrolyte and activated carbon powders(ACP) hybridized with the composite as a polrizable electrode. The cyclic voltammogram of the electric double-layer capacitor fabricated demonstrated that electric charge was stored in the elecric double-layer at the interface between the polarizable electrode and the electrolyte. The value of capacitance of the capacitor was 10 F/(gram of total ACP), which was comparable to that of the capacitors using conventional liquid electrolytes.

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Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis

  • Yeon, Jinouk;Park, Jong-Sug;Lee, Sang Ho;Lee, Kyeong-Ryeol;Yi, Hankuil
    • Journal of Plant Biotechnology
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    • v.46 no.4
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    • pp.282-290
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    • 2019
  • Cuphea viscosissima plants accumulate medium-chain fatty acids (MCFAs), i.e., those containing 8 ~ 14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl-ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.

RADIATION MONITORING SYSTEM FOR ADVANCED SPENT FUEL CONDITIONING PROCESS FACITLITY

  • Kook Dong-Hak;Choung Won-Myung;Lee Eun-Pyo;You Gil-Sung;Cho Il-Je;Kwon Kie-Chan;Lee Won-Kyoung;Ku Jeoung-Hoe
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.11b
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    • pp.149-155
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    • 2005
  • The $ACP^1$ is under development for effective management of spent fuel by converting $UO_2$ into U-metal. For demonstration of this process, $\alpha-\gamma$ type new hotcell was built in the $IMEF^2$ basement. To secure against radiation hazard, this facility needs radiation monitoring system which will observe the entire operating area before the hotcell and service area at back of it. This system consists of 7 parts; Area Monitor for $\gamma$-ray, Room Air Monitor for particulate and iodine in both area, Hotcell Monitor for hotcell inside high radiation and rear door interlock, Duct Monitor for particulate of outlet ventilation, Iodine Monitor for iodine of outlet duct, CCTV for watching workers and material movement, Server for management of whole monitoring system. After installation and test of this, radiation monitoring system will be expected to assist the successful ACP demonstration.

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Isolation and Identification of Short Term Drought-Induced Genes in Zea mays L. Leaves

  • Rahman, Md. Atikur;Lee, Sang-Hoon;Choi, Gi Jun;Ji, Hee Jung;Kim, Won Ho;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.237-241
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    • 2017
  • Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

Optimization of Atmospheric Cold Plasma Treatment with Different Gases for Reduction of Escherichia coli in Wheat Flour

  • Lee, Jeongmin;Park, Seul-Ki;Korber, Darren;Baik, Oon-Doo
    • Journal of Microbiology and Biotechnology
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    • v.32 no.6
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    • pp.768-775
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    • 2022
  • In this study we aimed to derive the response surface models for Escherichia coli reduction in wheat flour using atmospheric cold plasma (ACP) with three types of gas. The jet-type atmospheric cold plasma wand system was used with a 30 W power supply, and three gases (argon, air, and nitrogen) were applied as the treatment gas. The operating parameters for process optimization considered were wheat flour mass (g), treatment time (min), and gas flow rate (L/min). The wheat flour samples were artificially contaminated with E. coli at a concentration of 9.25 ± 0.74 log CFU/g. ACP treatments with argon, air, and nitrogen resulted in 2.66, 4.21, and 5.55 log CFU/g reduction of E. coli, respectively, in wheat flour under optimized conditions. The optimized conditions to reduce E. coli were 0.5 g of the flour mass, 15 min of treatment time, and 0.20 L/min of nitrogen gas flow rate, and the predicted highest reduction level from modeling was 5.63 log CFU/g.

COMPARATIVE STUDY OF THE REMINERALIZATION EFFECTS USING CPP-ACP AND FLUORIDE ON THE ARTIFICIAL ENAMEL LESION (CPP-ACP 함유 크림과 불소가 초기 인공우식 법랑질에 미치는 재광화 효과에 대한 비교 연구)

  • Kim, Ki-Baek;Choi, Nam-Ki;Kim, Seon-Mi;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.36 no.1
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    • pp.38-45
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    • 2009
  • The aim of this study was to examine efficacy of the commercially available 10% CPP-ACP cream (Tooth mousse, GC Co., Japan) and/or 0.05% NaF solution on the remineralization of artifical caries-like lesion in the bovine teeth enamel. Sixty bovine teeth were embedded in orthodontic resin and flattened. The enamel surface in 3 mm diameter was exposed with nail varnish. Specimens were stored in demineralizing solution and divided 5 groups; Group 1 (No treatment), 2 (0.05% NaF solution 1 min), 3 (Tooth mousse 3 min), 4 (After 0.05% NaF solution, Tooth mousse treatment), and 5 (After Tooth mousse treatment, soaking in 0.05% NaF solution during 1 min). After treatment by groups, all specimens was stored in artificial saliva for 30 min. After the process described as above was performed during 10 days without pH cycling, surface hardness (Vickers Hardness Number, VHN) was tested and analyzed by paired t-test and one-way ANOVA test with SPSS 14.0. In intragroup comparison between surface hardness of pre and post-treatment, group 3, 4, 5 showed statistically significant increase (P < 0.05). In intergroup comparison among surface hardness increase of all groups, difference of group 5 between pre and post-treatment ($15.80{\pm}12.21$) was the highest, and followed by group 4 ($14.27{\pm}11.73$), 3 ($4.05{\pm}5.18$), 2 ($1.15{\pm}6.83$), 1 ($0.78{\pm}6.21$). Tooth mousse can be a good alternative agent for the fluoride, and the combination use with fluoride might have the additional anticariogenic effect.

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Influence of Cerebral Protection Methods in Thoracic Aortic Surgery Using Hypothermic Circulatory Arrest (저체온 순환정지를 이용한 흉부 대동맥 수술 시 뇌관류 방법에 따른 수술결과)

  • Kim, Jae-Hyun;Na, Chan-Young;Oh, Sam-Sae
    • Journal of Chest Surgery
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    • v.41 no.2
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    • pp.229-238
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    • 2008
  • Background: Protection of the brain is a major concern during thoracic aortic surgery using hypothermic circulatory arrest (HCA). This study compares the surgical outcomes of two different cerebral protection methods in thoracic aortic surgery using HCA: retrograde cerebral protection (RCP) and antegrade cerebral protection (ACP). Material and Method: We retrospectively reviewed data on 146 patients who underwent thoracic aortic surgery from May 1995 to February 2007 using either RCP (114 patients, Group 1) or ACP (32 patients, Group 2) during HCA. There were 104 dissections (94 acute and 10 chronic) and 42 aneurysms (41 true aneurysms and 1 pseudoaneurysm), and all patients underwent ascending aortic replacement. There were 33 cases of hemiarch replacement, 5 of partial arch replacement, and 21 of total arch replacement. Result: The two groups were similar in preoperative and operative characteristics, but Group 2 had more elderly (over 70 years old) patients (34.4% vs. 10.5%), more coronary artery diseases (18.8% vs. 4.4%), more total arch replacements (46.9% vs. 5.3%) and longer HCA time ($50{\pm}24$ minutes vs. $32{\pm}17$ minutes) than Group 1. The operative mortality was 4.4% (5/114) and 3.1% (1/32), the incidence of permanent neurologic deficits was 5.3% (6/114) and 3.1% (1/32), and the incidence of temporary neurologic deficits was 1.8% (2/114) and 9.4% (3/32) in Groups 1 and 2, respectively. There were no statistical differences between the two groups in operative mortality, postoperative bleeding, or neurologic deficits (permanent and temporary). Conclusion: The early outcomes of aortic surgery using HCA were favorable and showed no statistical difference between RCP and ACP. However, the ACP patients endured longer HCA times and more extended arch surgeries. ACP is the preferred brain protection technique when longer HCA time is expected or extended arch replacement is needed.