• Title/Summary/Keyword: acid sulfate

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Variation in Microbial Biomass and Community Structure in Sediments of Peter the Great Bay (Sea of Japan/East Sea), as Estimated from Fatty Acid Biomarkers

  • Zhukova Natalia V.
    • Ocean Science Journal
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    • v.40 no.3
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    • pp.145-153
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    • 2005
  • Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria - were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.

Microbial Amelioration of Acid Mine Drainage Impaired Soil using the Bacterial Consortia of Klebsiella sp. and Raoultella sp.

  • Park, Seon Yeong;Lee, Gi Won;Kim, Chang Gyun
    • Journal of Soil and Groundwater Environment
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    • v.26 no.1
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    • pp.34-44
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    • 2021
  • Acid mine drainage (AMD) resulting from pyrite oxidation in mining areas, subsequently leads to soil acidification accompanied by lowering pH and high concentration of metals and metalloids in its surrounding environment. Regarding to this, the microbial amelioration has been considered as a promising option for a more cost-effective and eco-friendlier countermeasure, compared to the use of alkaline chemicals. This study was aimed to evaluate influencing factors in microbially-mediated amelioration of acidic soil spiked by simulated AMD. For this, microcosm experiments were conducted by acid-neutralizing bacterial consortium (dominated by Klebsiella sp. and Raoultella sp.) under the various conditions of AMD spikes (0-2,500 mg SO42-/L), together with acidic mine soil (0-100 g) or sphagnum peat (0-5 g) in the 200 mL of nutrient medium. The employed bacterial consortium, capable of resisting to high level of sulfate concentration (up to 1,500 mg SO42-/L) in low pH, generated the ammonium while concomitantly reduced the sulfate, subsequently contributing to the effective soil stabilization with an evolution of soil pH up to neutral. Furthermore, it demonstrates that suitable condition has to be tuned for successful microbial metabolism to facilitate with neutralization during practical application.

Influence of Cement types on the Resistance to Acid and Sulfate (산 및 황산염 저항성에 미치는 시멘트 종류의 영향)

  • Park, Jae-Im;Bae, Su-Ho;Yu, Kyung-Geun;Lee, Kwang-Myong;Cha, Soo-Won;Chol, Sung
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.11a
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    • pp.421-424
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    • 2008
  • The purpose of this experimental research is to investigate the influence of cement types on the resistance to acid and sulfate. For this purpose, concrete specimens with three types of cement such as ordinary portland cement(OPC), binary blended cement(BBC), and ternary blended cement(TBC) were made for water-binder(W/B) ratios of 32% and 43%, and then according to JSTM C 7401, the appearance change and ratio of mass change of them were estimated through the immersion tests by 5% sulfuric acid, 10% sodium sulfate, and 10% magnesium sulfate solution, respectively. It was observed from the test result that the resistance against acid and sulfate increased with decreasing W/B ratio and those of BBC and TBC concretes were better than the case of OPC concrete from immersion tests of 91 days.

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Fabrication of Three-Dimensional Network Structures by an Electrochemical Method (전기화학적 방법을 통한 3차원 금속 다공성 막의 제조)

  • Kang, Dae-Keun;Heo, Jung-Ho;Shin, Heon-Cheol
    • Korean Journal of Materials Research
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    • v.18 no.3
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    • pp.163-168
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    • 2008
  • The morphology of three-dimensional (3D) cross-linked electrodeposits of copper and tin was investigated as a function of the content of metal sulfate and acetic acid in a deposition bath. The composition of copper sulfate had little effect on the overall copper network structure, whereas that of tin sulfate produced significant differences in the tin network structure. The effect of the metal sulfate content on the copper and tin network is discussed in terms of whether or not hydrogen evolution occurs on electrodeposits. In addition, the hydrophobic additive, i.e., acetic acid, which suppresses the coalescence of evolved hydrogen bubbles and thereby makes the pore size controllable, proved to be detrimental to the formation of a well-defined network structure. This led to a non-uniform or discontinuous copper network. This implies that acetic acid critically retards the electrodeposition of copper.

홍어연골을 이용한 저 어취 chondroitin sulfate의 제조

  • 최주현;조승목;박현덕;김성구;지청일;이양봉;김선봉
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.105-106
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    • 2003
  • 콘드로이틴황산(Chondroitin sulfate)은 생물체내에 널리 분포하는 mucopoly-saccharide의 일종으로 N-acetyl galactosamine과 glucuronic acid의 이당체 단위가 반복적으로 되풀이되는 구조를 가지며, 황산기의 결합 위치에 따라 A타입(4-sulfate) 및 C타입(6-sulfate) 등으로 나누어진다(Mucci et al., 2000). 콘드로이틴황산은 피부노화방지, 골 형성단백질, 항종양 등의 생리활성작용이 우수하고(Bayliss et al., 1999 ; Ha et al., 1999), 특히 퇴행성관절염에 효과(Omata et al., 2000)가 높아 최근 화장품, 건강보조 식품 및 의약품으로 이용되고 있다. (중략)

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Synthesis of Fibrous Gypsum from By-Product Gypsum fo Phosphoric Acid Process (인산 석고로부터 섬유상 반수석고의 생성)

  • 배동식;이구종;최상흘
    • Journal of the Korean Ceramic Society
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    • v.27 no.5
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    • pp.577-582
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    • 1990
  • The synthesis conditions of fibrous calcium sulfate hemihydrate were investigated by using phosphogypsum and calcium sulfate hemihydrate. The unstable organogel was deposited by adding methanol to the saturated solution with gypsum at ageing temperature, and it was crystallized to fibrous gypsum hemihydrate while methanol was removed by rapid filtrating. In case of using calcium sulfate hemihydrate, fibrous $\beta$-calcium sulfate hemihydrate was formed by adding methanol of 67% to saturated solution at 6$0^{\circ}C$ and ageing for 5 minutes and filtering with suction. Minor components in phosphogypsum did not affect the reaction.

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Isolation of High-molecular-weight-compound degrading microorganisms and sulfate reducing Bacteria involved in Composting Process (퇴비화 과정에 관여하는 생체 고분자 분해 미생물 및 황산 환원균의 분리)

  • Lee, Seong-Taek;Lee, Jae-Jeong;Na, Hyun-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.2
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    • pp.31-37
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    • 1994
  • For a microbiological study of composting process, screening and assay method for biopolymer degrading enzymes and microorganisms were developed and for the study of the possibility of composting in anaerobic state, distribution of sulfate reducing bacteria which plays a final role in anaerobic degradation was investigated. Substrates used for the development of assay methods for biopolymer degradation are ${\beta}-glucan$, xylan, dextran, CMC(carboxy methly cellulose), casein, and collagen. These substrates were made insoluble by a cross-linking agent and linked with dye to make chromogenic substrates. ${\beta}-glucan$ and xylan substrates could substitute congo-red method for screening of polymer degrading microorganisms without damaging the colonies. Sulfate reducing bacteria contained in the sample sludge showed preference to lactic acid, propionic acid, butyric acid and formic acid and could use acetic acid and valeric acid.

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A Comparison of shear Bonding Strength with Polyacrylic acid and Phosphoric acid Enamel Surface Conditioning (폴리아크릴산과 인산으로 법랑질표면 처리후 전단결합강도의 비교)

  • Roh, Joung-Sub;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.32 no.1 s.90
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    • pp.51-57
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    • 2002
  • The purpose of this study was to compare in vitro shear bonding strength with three different enamel surface preparations (1) 30% sulfated polyacrylic acid with 0.3M lithium sulfate (2) 40% sulfated polyacrylic acid with 0.3M lithium sulfate (3) 37% phosphoric acid. 105 extracted human premolar teeth were divided into each three groups of 35. Metal brackets were bonded to teeth in the three groups. The same self curing resin was used for all groups. A shearing force was applied to the teeth. After debonding, bases of bracket and enamel surfaces were examined under steroscopic microscope to determine the failure modes. Statistical analysis of the data was carried out with one way ANOVA and Student t- test. The results were as follows. 1. Shear bond strength values for the 30% polyacrylic acid and 40% polyacrylic acid group were approximately two thirds of the phosphoric acid group. It maintains clinically acceptable but not enough bond strength. 2. There was no statistically significant difference in shear bond strengths between 30% and 40% polyacrylic acid group. 3. The failure modes of brackets had some differences. In polyacrylic acid groups, the percentage of adhesive/enamel failure was higher than that of adhesive/ bracket interface failure. On the contrary in phosphoric acid groups, the results were reversed. Further study of bond strength could be required. If polyacrylic acid enamel conditioning is used clinically.

Effects of Glycosaminoglycan on the Development of In vitro Fertilized Mouse Embryo (Glycosaminoglycan이 생쥐 수정란의 체외 발달에 미치는 영향)

  • Kim, J.W.;Seo, D.S.;Yoon, S.H.;Ko, Y.
    • Korean Journal of Animal Reproduction
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    • v.24 no.3
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    • pp.269-279
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    • 2000
  • The present study was carried out to evaluate the effect of glycosaminoglycans added to the culture medium on the mouse embryo development to the blastocyst stage. In vitro fertilized mouse oocytes were cultured in Ham's F-10 supplemented with 10% FBS either in the absence or presence of 0.1, 0.5, 1.0 mg/$m\ell$ hyaluronic acid, chondroitin sulfate, and dermatan sulfate, respectively. After 4 days in culture, embryos developed to blastocysts were observed in all groups. There was a significant increase in blastocyst yield in the presence of hyaluronic acid and chondroitin sulfate (p<0.05), whereas dermatan sulfate was ineffective. Development to the blastocyst stage was best supported in 0.1, 0.5, 1.0 mg/$m\ell$ hyaluronic acid and 0.5mg/$m\ell$ chondroitin sulfate. It is concluded that hyaluronic acid and chondroitin sulfate support the development of mouse oocyte fertilized in vitro to the blastocyst stage. Furthermore, these results suggest that glycosaminoglycans can be utilized to support embryo development in vitro as a nutrient instead of serum.

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계면활성제를 이용한 우지지방산을 포화지방산과 불포화 지방산의 분리

  • 이옥섭;김점식
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.10 no.1
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    • pp.24-41
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    • 1984
  • Tallow fatty acid consists of mixtures of fatty acids differing in chain length and saturation. In separation of tallow fatty acid, the effects of the type and concentration of detergents and electrolytes were studied. And the changes of acid composition of particular fractions were determined by gas-chromatography. Sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES) and sodium lauryl benzene sulfonate (SLBS) were used as detergents and NaCl, Na2SO4 and MgSO4 were used as electrolytes. At low concentration of detergent, the tallow fatty acid was not fully wetted, and at high concentration, the emulsion was so stable that the tallow fatty acid was not well separated. The addition of proper amount of electrolyte increased the separation efficiency by the decrease of interfacial tension and by the increase of the amount of adsorbed detergent on the surface of solid fatty acid crystals. The optimum range of detergent was 0.4-0.6% (wt.) in SLS, 0.2-0.4% in SLES and 2.0-) .0% in SLBS. And the optimum range of electrolyte was 2.0-2.5% in NaCl, 3.0-4.0% In Na2SO4 and 0.5-1.0% in MgSO4 respectively.

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