• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1999년도 학술발표회 진행표 및 논문초록
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• pp.66-66
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• 1999

The negative chronotropic effect of ACh on heart fades in the continuous presence of ACh, which is known as a phenomenon called "vagal escape". The underlying mechanism of vagal escape is not entirely clear, but desensitization of acetylcholine-activated $K^{＋}$ currents ($K_{ACh}$) was suggested, at least in part, to be responsible.(omitted)d)

• 한국동물학회지
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• 제12권2호
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• pp.35-38
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• 1969

1. Ach에 대한 드렁허리의 감수성은 가장 민감한 자료로 알려진 가물치 보다 10배 이상 민감하므로 Ach의 생물학적 정량에 가장 우수한 재료로 생각된다. 2. 5-HT는 미꾸라지에서는 심장활동을 강하게 촉진하였으나 드렁허리에 있어서는 오히려 심장활동을 억제함을 알 수 있었다. 3. Norepinephrine에 대한 드렁허리의 반응은 epinephrine에 대한 반응 보다 100배 이상 민감하다.

• Environmental Analysis Health and Toxicology
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• 제18권2호
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• pp.155-163
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• 2003

Fluoranthene, a common polycyclicaromatic hydrocarbon (PAH), exhibits phototoxicity which may affect aquatic organisms. The eventual goal of this study is to develop a biomarker of Japanese medaka (Oryzias latipes) used in monitoring hazardous chemicals in the ecosystem. In this study we investigated neural toxicity of fluoranthene in Japanese medaka (Oryzias latipes) along with comparative analysis of corresponding behavioral response. The untreated individuals shooed normal behavioral characteristics (i. e., smooth and linear movements). The treated fish, however, showed stopping and abrupt change of orientation (100 ppb), and severely reduced locomotive activity and enhanced surfacing activity (1,000 ppb). Treatment of the medaka fish with fluoranthene caused a significant suppresson of acetycholine esterase (AChE) activities in the body portion but not in the head portion. When fish were exposed to 1,000 ppb of fluoranthene for 24 hr, the body AChE activities decreased from 126.${\pm}$31.89 (nmoles substrate hydrolyzed per min per mg protein) to 49.51${\pm}$11.99. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head and body portions were comparatively analyzed using an immunohistochemical technique. Five organs of the medaka fish showing a strong TH protein expression were the olfactory bulb, hypothalamus, optic lobe, pons and myelencephalon regions. This study provides molecular and neurobehavioral bases of a biomonitoring system for toxic chemicals using fish as a model organism.

• 약학회지
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• 제39권3호
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• pp.231-242
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• 1995

Electroconvulsive shock (ECS) increases the activity of acetylchohnesterase and decreases in brain acetylcholine levels. A large amount of free fatty acids accumulated in the brain tissue affects cerebral blood flow, brain edema and inflammation and results in brain injury. The present study examined the effect of docosahexaenoic acid (DHA) and D,L-pyroglutamic acid (D,L-PCA) on the learning and memory deficit using the passive avoidance failure technique and on the change of acetylcholine and choline level in the cerebral cortex of ECS-induced mice. The application of ECS (25mA, 0.5sec) induced a significant decrease in memory function for 30 min. ECS-induced a significant decrease in cortical acetylcholine and choline levels 1 min following the ECS application, which were almost recovered to ECS control level after 30 min. DHA (20 mg/kg, i.p.). administered 24 hr before shock. prevented the ECS-induced passive avoidance failure and the decrease of acetylcholine level 1 min following the ECS application. DHA failed to elicit a change in cortical choline level. DHA did not affect memory function and the cortical Ach and choline level of normal mice. The administration of D,L-PCA (500 mg/kg, i.p.) increased the effect of DHA on memory function and the change of cortical acetylcholine level of ECS induced mice. These results suggest that DHA treatment may be contributed to the prevention against memory deficit, and to the activation of cholinergic system in the ECS induced mice.

• 한국임상수의학회지
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• 제22권4호
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• pp.392-395
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• 2005

체중 32kg의 3년령 암컷 독일셰퍼드가 식이역류와 구토로 내원하였다. 신체검사에서 체중감소, 음성변화, 유연 및 후구유약이 관찰되었다. 방사선검사에서 거대식도가 관찰되었다. Neostigmine challenge test는 음성이었으며 acetyle\choline receptor에 대한 항체가는 1.58nmol/L로 양성이었다. 따라서 후천성 중증 근육무력증의 만성형으로 진단하였다. Pyridostigmine bromide 1mg/kg을 1일 2회 투여하여 치료하여 후구의 유약 증상은 현저히 개선되었고, 정상보행을 보였다. 치료과정중 이물성 폐렴이 발생하였고, 항생제 치료를 실시하였으나 관리의 어려움과 경제적 문제로 인한 보호자의 요구로 안락사 시켰다.

• Applied Biological Chemistry
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• 제50권2호
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• pp.127-131
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• 2007

일련의 새로운 3-benzylidenemyosmine 유도체들의 구조 변화와 미국 바퀴벌래(Periplaneta. americana L.)의 nicotin acetylcholine 수용체 (nAChRs) 사이의 결합 친화력 상수에 관한 정량적인 구조와 활성과의 관계를 분자 홀로그램(H) QSAR 방법으로 검토하였다. 친화력 상수에 관하여 가장 양호한 HQSAR 모델은 분자조각 크기 5${\sim}$8 bin 조건에서 유도된 모델(IV-2)이었다. HQSAR 모델(VI-2)은 높은 예측성(q$^2$=0.507)과 상관성(r$^2_{nev.}$=0.944)에 근거하여 양호한 통계값들을 나타내었다. 그리고 HQSAR 기여도로부터 결합 친화력 상수는 분자내 anabaseine 고리에 의존적이었으며 결합 친화력성이 높은 화합물들이 최적화된 모델(VI-2)에 의하여 설계되었다.