• Title/Summary/Keyword: acetic acid fermentation

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Studies on the Acetic Acid Fermentation Using Maesil Juice (매실을 이용한 식초산 발효에 관한 연구)

  • 김용두;강성훈;강성구
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.4
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    • pp.695-700
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    • 1996
  • In other to produce vinegar using maesil, acetic acid bacteria were selected from several conventional vinegars, and total 16 acetic acid producing bacterial strains were isolated. Among the isolated strains, a strain was selected from the medium with maesil juice which showed the highest productivity of acetic acid. The strain was identified as Acetobacter sp. VC-2 and it's cultural characteristics were also investigated in the batch shake flasks. The medium containing 2% acetic acid, 6% ethanol, 0.2% glucose, 0.3% yeast extract and 30% maesil juice was suitable for acetic acid production with Acetobacter sp. VC-2 at 3$0^{\circ}C$. The acidity of culture medium was reached to 6.5% after 8 days shaking cultivation at 3$0^{\circ}C$. The main components of organic acids were acetic, citric, malic and tartaric acid, and they were 4.28, 1.38, 0.48 and 0.30%, respectively.

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Biohydrogen Production from Sugar Manufacturing Wastewater and Analysis of Microbial Diversity (제당폐수를 이용한 수소생산과 미생물의 군집해석)

  • Lee, Heesu;Lee, Tae-Jin
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.3
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    • pp.41-51
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    • 2012
  • Biohydrogen production and analysis of microbial community were attempted from the sugar manufacturing wastewater with anaerobic fermentation process. Addtion of nutrients ($N{\cdot}P$) into sugar manufacturing wastewater stimulates hydrogen production from 9.53 to $26.67m{\ell}$ $H_2/g$ COD. Butyric acid, acetic acid, lactic acid, and propionic acid were detected in the sample of the anaerobic fermentation process. Butyric acid/Acetic acid(B/A) ratio was increased 0.50 to 0.92 according to the nutrients addtion into the wastewater. Microbial community was analyzed as Clostridium sp. in the phylum of Firmicutes and Klebsiella sp., Erwinia sp., and enterobacter sp. of the class of $\gamma$-Proteobacteria. As the improvement of hydrogen production, Erwinia sp. was decreased and Klebsiella sp. was increased.

Effect of Ethanol on the Production of Cellulose and Acetic Acid by Gluconacetobacter persimmonensis KJ145 (Gluconacetobacter persimmonensis KJ145를 이용한 Bacterial Cellulose 및 초산발효에 미치는 Ethanol의 영향)

  • 이오석;장세영;정용진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.2
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    • pp.181-184
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    • 2003
  • We investigated the effect of ethanol on the production of cellulose and acetic acid fermentation by Gluconacetobacter persimmonensis KJ145. Results showed that bacterial cellulose productivity was highest when 2% ethyl alcohol was added to apple-juice medium. For acetic acid production, 7% ethyl alcohol was needed. Optimal concentration of ethyl alcohol was 5% for simultaneous production of bacterial cellulose and acetic acid. For simultaneous production of bacterial cellulose and acetic acid, optimal nitrogen source and optimal concentration were corn steep liquor and 15% (w/v), respectively Optimal culture time for simultaneous production of bacterial cellulose and acetic acid was 14 days. At the optimal condition, Cluconacetobacter persimmonenis KJ145 produced 7.55 g/L of bacterial cellulose (dry weight).

Application of Emulsion Liquid Membrane to Removal of Fermentation Inhibitors from Simulated Hemicellulosic Hydrolysates (모사 헤미셀룰로오스 가수분해액으로부터 발효 저해물질의 제거를 위해 에멀젼형 액막법의 적용)

  • Lee, Sang Cheol
    • Korean Chemical Engineering Research
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    • v.53 no.4
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    • pp.457-462
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    • 2015
  • Hemicellulosic hydrolysates contain not only sugars but also several kinds of ethanol fermentation inhibitory substances such as carboxylic acids, furans and phenolic compounds. In this work, emulsion liquid membrane (ELM) was chosen as a separation technology to remove the inhibitors. A basic simulated hemicellulosic hydrolysate was composed of xylose as sugar, dilute sulfuric acid solution as solvent, and acetic acid as carboxylic acid, and furfural as furan derivative or p-hydroxybenzoic acid(HBA) as phenolic compound was added to the hydrolysate when necessary. Acetic acid and HBA as weak acid could be selectively removed from the hydrolysates in all the ELM systems considered here, but furfural as aldehyde was quite hard to remove. Also, when HBA was added to the basic simulated hemicellulosic hydrolysate, both of acetic acid and HBA in the feed phase could be selectively removed up to 99% in an ELM system with tributyl phosphate as extractant.

Studies on the Utilization of Orange Peel in the Spirit Vinegar Brewing (식초양조(食醋釀造)에 있어 밀감과피즙(果皮汁) 이용(利用)에 관(關)한 연구(硏究))

  • Kim, Yong Ho;Park, Yoon Joong;Sohn, Cheon Bae
    • Korean Journal of Agricultural Science
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    • v.8 no.1
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    • pp.108-116
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    • 1981
  • A study was carried out to get the basic information about the brewing of spirit vineger from medium containing mandarin orange peel, and the results obtained were as follows. 1. The yield of peel to fruit was 29.0%. 2. The optimum concentration of peel extract for the acetic acid fermentation medium was about 25%. 3. Acetic fermentation was inhibited when the peel extract content of medium was over 70%. Also the maximum acidity of the medium which contained 90% of peel extract was declined up to 1% comparing to the medium contained 25% of peel extract. 4. In the acetic acid fermentation of medium containing 25% of orange peel extract under the aerobic condition, the average rate of acetic acid production was 0.062g/100ml. hr. and the rate of acetic acid production in log phase was 0.15g/100ml. hr. also the yield of product based on acetification was 91.4% 5. Oxalate, pyruvate, malate was detected in acetic acid fermentation medium. 6. The quality of vineger made from medium containing 25% of orange peel extract was good.

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Quality characteristics of vinegar fermented with different amounts of black garlic and alcohol (흑마늘의 첨가량과 초기 알코올 농도를 달리한 발효 식초의 품질특성)

  • Seo, Weon Tack;Choi, Myoung Hyo;Sim, Hye Jin;Kim, Gyeong Wha;Shin, Yeon Mi;Kang, Min Jung
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.34-41
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    • 2016
  • The quality characteristics of black garlic vinegar produced by acetic acid fermentation were analyzed over a 25 day period. Black garlic vinegar was produced using black garlic 3, 6, 9 and 12 folds with water, and initial alcohol concentrations of 6~12%. The pH values of the vinegar samples were in range of 3.85~4.46 after 25 days fermentation, and the sugar contents were highest after 9 days of fermentation ($12.00{\sim}13.50^{\circ}Brix$). The total polyphenol concents of vinegars were in the range of 20.65~52.04 mg/100 mL 25 days fermentation. Pyrogallol and vanillic acid were detected in the completely fermented vinegars, and their contents increased with black garlic content. Five organic acids (lactic acid, acetic acid, citric acid, succinic acid and propionic acid) were detected after 25 days of fermentation, with the total amount of organic acids being 5,327.34~6,307.64 mg/100 mL, and the total abetic acid contents beging the highest of the organic acids at 4208.90~5,570.29 mg/100 mL. After 25 days of fermentation, the total minerals contents were higher, and depending on the quantity of black garlic added. Of the minerals, the of potassium content was the highest (366.23~1,619.67 mg/L).

The Quality Comparison of Grape Vinegar by Two Stages Fermentation with Traditional Grape Vinegar (2단계 발효에 의한 포도식초와 재래식 포도식초의 품질 비교)

  • 정용진;이명희;서권일;김주남;이용수
    • Journal of the East Asian Society of Dietary Life
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    • v.8 no.4
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    • pp.462-468
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    • 1998
  • 편이었다. 미량성분으로는 K이 다량으로 존재하였고, 2단계 발효로 제조된 포도식초는 포도양파식초 보다K, Na, Cu함량이 높게 나타났다. ^x Grape vinegar (A) and grape onion vinegar(B) added (3% of onion juice) were produced through two stages of fermentation(alcohol fermentation and acetic acid fermentation) to increase the grape's use. Grape wine which contained 5.6% alcohol was produced on the 3rd day of first stage. Then through the second stages, grape vinegar, of which total acidity was 5.37% was produced. The quality of (A) and ( B) which was produced through the two stages of fermentation was compared with the traditional grape vinegar (C, D) in the market. The content of sugar in (D) was a little higher such as 5.4 $^{\circ}$Brix than others. That of (A) and (B) was 5.13, 4.98 $^{\circ}$Brix respectively. The content of remaining alcohol in (C) was high such as 0.23% comparatively. But there was no remaining alcohol in (A) and (B). The content of acetic acid was 4.3~5.3% as a major organic acid of vinegars. The content of tartaric acid was 340.0 in (A), 315.7 in (B), 322.6 in (C) and 391.7mg% in (D). The content of lactic acid was distinctly high such as 277.4mg% in (D) There were differences such as 9.2~15.5mg% in the content of total free amino acids among grape vinegars. (D) contained 15.5mg% of total free amino acid and (B) also highly such as 12.0mg%. Potassium was high in grape vinegars. The content of potassium, sodium and copper was higher in (A) than (B).

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Change of Aroma Compounds during Corn Vinegar Ripening (숙성에 의한 옥수수 식초의 향기 성분 변화)

  • Shin, In-Ung;Park, Eun-Hee;Choi, Yeong-Hwan;Ryu, Su-Jin;Shin, Woo-Chang;Kim, Myoung-Dong
    • Microbiology and Biotechnology Letters
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    • v.48 no.1
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    • pp.90-97
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    • 2020
  • Vinegar was prepared from corn produced in Gangwon province and changes to the major aroma components were investigated for acetic acid fermentation and ripening. The most prevalent volatile component in corn alcohol was determined to be 2-phenylethanol (accounting for 27% of total aroma components); the levels of 2-phenylethanol decreased to 15% after acetic acid fermentation. Principal component analysis of volatile compounds revealed that corn vinegar was distinguishable from corn alcohol. The highest content of total volatile components (50%) was acetic acid, followed by 2-phenylethanol and diethyl succinate. Ethyl phenylacetate and diethyl succinate had the highest odor activity value, and might contribute most profoundly to the aroma of corn vinegar.

Effects of Sugars Addition in Alcohol Fermentation of Oriental Melon (첨가 당의 종류가 참외의 알코올발효에 미치는 영향)

  • Jo, Yong-Jun;Jang, Se-Young;Kim, Ok-Mi;Park, Chan-Woo;Jeong, Yong-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.9
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    • pp.1359-1365
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    • 2010
  • This study investigated effects of types of added sugar on alcohol fermentation of oriental melon. According to the results, pH was not significantly different according to types of added sugar and fermentation process. Total acidity increased with fermentation process in all groups by recording around 1.4% at the ninth day of fermentation. For sugar content, its initial level was $22^{\circ}Brix$, and alcohol fermented oriental melon fluids added by sucrose, fructose and glucose recorded similar levels or $6.6{\sim}6.8^{\circ}Brix$ while the fluids added by honey and fructo-oligosaccharide showed a slightly higher level or $8.1^{\circ}Brix$ at the ninth day of fermentation. Although free sugar content was different in the early phase of fermentation according to types of added sugar such as sucrose, fructose and glucose, it reduced with fermentation process to nearly non-detection at the ninth day of fermentation. As organic acids, lactic acid and acetic acid were observed in all phases of fermentation and their contents became higher gradually with fermentation process. Alcohol content showed the highest level in alcohol fermented oriental melon fluid added by sucrose by recording 12.80% and was relatively low in the fluids added by fructose and oligosaccharide. For alcohol, acetaldehyde, n-propanol and iso- amylalcohol contents were not significantly different according to types of added sugar and methanol content was the lowest in the fluid added by fructose by recording 84.99 ppm.

Studies on the Acetic Acid Fermentation (아세트산발효에 관한 연구)

  • 노완섭
    • Microbiology and Biotechnology Letters
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    • v.6 no.3
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    • pp.115-120
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    • 1978
  • The manufacture of vinegar provides a means of utilizing the juices of many fruits and various kinds of cereals as well as of starchy vegetables. However, no successful attempt has been made to utilize the glutinous rice powder, which is discussed from the Mi Gwa (made of glutinous rice) making, for the manufacture of vinegar. After alcoholic fermentation of the glutinous rice power, it was devided to ferment the liquor to vinegar and to examine factors affecting this fermentation. A laboratory typed vinegar generator was used to convert the alcohol in fermented liquor to acetic acid. Recycling of the fermented liquor through the generator was found essential in this process. Among many strains of Acetobacter cultures used, which was selected from ordinary home made vinegar from all over the Korea, Acetobacter No. Aa-97 proved more efficient for this fermentation. Addition of 1% phosphate (K$_2$HPO$_4$) and 20% apple cider vinegar. to the fermented liquor increased both the rate and efficiency of acetic acid fermentation.

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