Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Quality characteristics of vinegar fermented with different amounts of black garlic and alcohol

흑마늘의 첨가량과 초기 알코올 농도를 달리한 발효 식초의 품질특성

  • Received : 2015.10.17
  • Accepted : 2015.12.14
  • Published : 2016.02.28
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Abstract

The quality characteristics of black garlic vinegar produced by acetic acid fermentation were analyzed over a 25 day period. Black garlic vinegar was produced using black garlic 3, 6, 9 and 12 folds with water, and initial alcohol concentrations of 6~12%. The pH values of the vinegar samples were in range of 3.85~4.46 after 25 days fermentation, and the sugar contents were highest after 9 days of fermentation ($12.00{\sim}13.50^{\circ}Brix$). The total polyphenol concents of vinegars were in the range of 20.65~52.04 mg/100 mL 25 days fermentation. Pyrogallol and vanillic acid were detected in the completely fermented vinegars, and their contents increased with black garlic content. Five organic acids (lactic acid, acetic acid, citric acid, succinic acid and propionic acid) were detected after 25 days of fermentation, with the total amount of organic acids being 5,327.34~6,307.64 mg/100 mL, and the total abetic acid contents beging the highest of the organic acids at 4208.90~5,570.29 mg/100 mL. After 25 days of fermentation, the total minerals contents were higher, and depending on the quantity of black garlic added. Of the minerals, the of potassium content was the highest (366.23~1,619.67 mg/L).

흑마늘에 가수량을 달리하여 3, 6, 9 및 12배가 되도록하여 알코올 발효시켜 초기 알코올 농도를(6~12%)로 달리한 흑마늘 식초를 25일간 초산발효 시키면서 발효과정 중 품질특성 분석을 위해 관련인자를 분석하였다. pH는 발효 중 점차 감소하여 25일 후에 3.85~4.46였고, 당도는 발효 9일 후에 $12.00{\sim}13.50^{\circ}Brix$로 가장 높았으나 이후 다시 감소하여 발효초기와 유사한 범위였다. 총 폴리페놀 화합물 함량은 발효 0일차에 19.67~50.46 mg/100 mL이던 것이 최종 25일에는 20.65~52.04 mg/100 mL의 범위로 미량 증가 하였다. 최종 숙성 완료된 흑마늘 식초에서 페놀화합물을 확인 동정한 결과 pyrogallol과 vanilic acid가 검출되었으며, 흑마늘의 농도가 높을수록 그 함량이 더 높았다. S-allyl cysteine(SAC) 함량은 6배 이상으로 가수희석된 흑마늘 식초에서는 검출되지 않았고, 흑마늘의 첨가량에 비례하여 그 함량이 높았는데 발효 기간에 따른 함량의 차이는 적었다. 유기산은 총 5종이 검출되었는데, 검출된 유기산 중 acetic acid의 함량이 가장 높아 발효가 완료된 25일차 시료에서 4,208.90~5,570.29 mg/100 mL이었고 유기산의 총량은 5,327.34~6,307.64 mg/100 mL의 범위였다. 발효 25일 후 흑마늘 식초의 무기물 함량은 K이 366.23~1619.67 mg/L로 가장 높았고, 흑마늘의 첨가량이 높을수록 총 함량이 더 높았다.

Keywords

References

  1. Adams MR (1998) Vinegar In: Microbiology of fermented food. Blackie Academic and Professional, London, UK, p 1-44
  2. Kim MJ, Choi JH, Kwon SH, Kim HD, Bang MH, Yang SA (2013) Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells. Korean J Food Sci Technol, 45, 350-355 https://doi.org/10.9721/KJFST.2013.45.3.350
  3. Jeong YJ, Lee MH (2000) A view and prospect of vinegar using kyungpook speccial products (persimmon, apple and vinegar). Food Ind Nutr, 5, 53-59
  4. Baek CH, Jeong DH, Baek SY, Choi JH, Park HY, Choi HS, Jeong ST, Kim JH, Jeong YJ, Kwon JH, Yeo SH (2013) Quality characteristics of farm-made brown rice vinegar via traditional static fermentation. Korean J Food Preserv, 20, 564-572 https://doi.org/10.11002/kjfp.2013.20.4.564
  5. Ann YG, Kim SK, Shin CS (2001) Studies on wax gourd - ginseng vinegar. Korean J Food Nutr, 14, 52-58
  6. Seo HB, Song YJ, Kang JY, Kwon DK, Kim PG, Ryu SP (2011) The study of persimmon vinegar as a functional drink on reduce blood lipids and enhance exercise perfomance. J Korean For Soc, 100, 232-239
  7. Na HS, Choi GC, Yang SI, Lee JH, Cho JY, Ma SJ, Kim JY (2013) Comparison of characteristics in commercial fermented vingars made with different ingredients. Korean J Jood Preserv, 20, 482-487 https://doi.org/10.11002/kjfp.2013.20.4.482
  8. Hong SM, Moon HS, Lee JH, Lee HI, Jeong JH, Lee MK, Seo KI (2012) Development of functional vinegar by using cucumbers. J Korean Soc Food Sci Nutr, 41, 927-935 https://doi.org/10.3746/jkfn.2012.41.7.927
  9. Cho JG (2008) Garlic and health. Food Preserv Proces Ind, 7, 2-8
  10. Jeong DH, Jeong SO (2005) Garlic science. World science, Seoul, Korea, p 6
  11. Bakri IM, Douglas CWI (2005) Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol, 50, 645-651 https://doi.org/10.1016/j.archoralbio.2004.12.002
  12. Clement F, Pramod SN, Venkatesh YP (2010) Identity of the immunomodulatory proteins from garlic (Allium sativum) with the major garlic lectins or agglutinins. Int Immunopharmacol, 10, 316-324 https://doi.org/10.1016/j.intimp.2009.12.002
  13. Pedraza-Chaverri J, Medina-Campos ON, Avila-Lombardo R, Berenice Zuniga-Bustos A, Orozco-Ibarra M (2006) Reactive oxygen species scavenging capacity of different cooked garlic preparation. Life Sci, 78, 761-770 https://doi.org/10.1016/j.lfs.2005.05.075
  14. Rose P, Whiteman M, Moore PK, Zhu YZ (2005) Bioactive S-alk(en)yl cysteine sulfoxide metabolites in the genus Allium: the chemistry of potential therapeutic agents. Nat Prod Rep, 22, 51-68
  15. Bae SE, Cho SY, Won DY, Lee SH, Park HJ (2012) A comparative study of the different analytical methods for analysis of S-allyl cysteine in black garlic by HPLC. LWT-Food Sci Technol, 46, 532-535 https://doi.org/10.1016/j.lwt.2011.11.013
  16. Nagatoshi I, Benjamin HS, Kenjiro R, Hiromichi M, Yoichi I (1999) Antioxidant effects of fructosyl arginine, a maillard reaction product in aged garlic extract. J Nutr Biochem, 10, 373-376
  17. Min JS, Kang BW, Park JU, Kim MJ, Lee HH, Ryu EJ, Joo WH, Kim KH, Jeong YK (2013) Effect of black garlic extract on cytokine generation of mouse spleen cells. J Life Sci, 23, 63-68 https://doi.org/10.5352/JLS.2013.23.1.63
  18. Kim GH, Kim JH, Yang JY (2014) Change in flavor components of black-fermented garlic wine according to the type of chips during the manufacturing process. J Food Hyg Safety, 29, 73-77 https://doi.org/10.13103/JFHS.2014.29.1.073
  19. Park CH, Kim KH, Kim NY, Kim SH, Yook HS (2014) Antioxidative capacity and quality characteristics of yanggaeng with fermented aged black giant garlic (Allium ampeloprasum L., var. ampeloprasum auct.) paste. Korean J Food Nutr, 27, 1014-1021 https://doi.org/10.9799/ksfan.2014.27.6.1014
  20. Sim HJ, Hwang CR, Kang MJ, Kim GM, Shin JH (2014) The quality and sensory characteristics of tofu with various levels of balck garlic extract. Korean J Food Preserv, 21, 688-693 https://doi.org/10.11002/kjfp.2014.21.5.688
  21. Jeong TS, Kim JH, An SA, Won YD, Lee SH (2013) Effect of black garlic on antioxidant activity and amino acids composition in Cheonggukjang. Korean J Food Preserv, 20, 643-649 https://doi.org/10.11002/kjfp.2013.20.5.643
  22. Folin O, Denis W (1915) A colorimetric method for determination of phenols (phenol derivatives) in urine. J Biol Chem, 22, 305-308.
  23. Lee SJ, Ryu JH, Kim RJ, Lee HJ, Sung NJ (2010) Effect of removed peel from sweet persimmon on nutritional ingredients and antioxidant activities. J Korean Soc Food Sci Nut, 39, 1495-1502 https://doi.org/10.3746/jkfn.2010.39.10.1495
  24. Yoon JR, Chang YH (2014) Quality characteristics of vinegar added with sea buckthorn (Hippophae rhamnoides) leaf extract and berry juice by different fermentation methods. Ph D Thesis, Myongji University, Korea, p 178
  25. Choi DJ, Lee SJ, Kang MJ, Cho HS, Sung NJ, Shin JH (2008) Physicochemical characteristics of black garlic (Allium sativum L.). J Korean Soc Food Sci Nutr, 37, 465-471 https://doi.org/10.3746/jkfn.2008.37.4.465
  26. Yi MR, Hwang JH, Oh YS, Oh HJ, Lim SB (2014) Quality characteristics and antioxidant activity of immature Citrus unshiu vinegar. J Korean Soc Food Sci Nutr, 43, 250-257 https://doi.org/10.3746/jkfn.2014.43.2.250
  27. Hong SM, Kang MJ, Lee JH, Jeong JH, Kwon SH, Seo KI (2012) Production of vinegar using Rubus coreanus and its antioxidant activities. Korean J Food Preserv, 19, 594-603 https://doi.org/10.11002/kjfp.2012.19.4.594
  28. Lee JC, Han WC, Lee JH, Jang KH (2012) Quality evaluation of vinegar manufactured using rice and Rosa rugosa Thunb. Korean J Food Sci Technol, 44, 202-206 https://doi.org/10.9721/KJFST.2012.44.2.202
  29. Murota K, Terao J (2003) Antioxidative flavonoid quercetin : implication of its intestinal absorption and metabolism. Arch Biochem Biophys, 417, 12-17 https://doi.org/10.1016/S0003-9861(03)00284-4
  30. Mei Y, Wei D, Liu J (2003) Reversal of cancer mutidrug resistance by tea polyphenol in KB cells. J Chemother, 15, 260-265 https://doi.org/10.1179/joc.2003.15.3.260
  31. Ha DH, Choi TJ, Yoo SM (2007) Effects of vanillic acid on the cell viability and melanogenesis in cultured human skin melanoma cells damaged by ROS-induced cytotoxicity. J Exp Biomed Sci, 13, 349-354
  32. Kim KJ, Do JR, Jo JH, Kim YM, Kim BS, Lim SD, Kang SN (2005) Antibacterial activity of Terminalia chebula Retz. extract against food spoilage microorganisms. Korean J Food Sci Technol, 37, 498-503
  33. Malcolm D, Elliott KAC (1930) The use of barcroft apparatus for the measurement of tissue respiration. Biochem J, 24, 820-833 https://doi.org/10.1042/bj0240820
  34. Amagase H (2006) Clarifying the real bioactive constituents of garlic. J Nutr, 136, 716-725 https://doi.org/10.1093/jn/136.3.716S
  35. Jung EB, Choi JH, Yu HJ, Kim KH, Lee SK, Hwang YI, Lee SH (2013) Organosulfur compounds in fermented garlic extracts and the effects on alcohol induced cytotoxicity in CYP2E1-transfected HepG2 cells. J Korean Soc Food Sci Nutr, 42, 342-347 https://doi.org/10.3746/jkfn.2013.42.3.342
  36. Tak HM, Kim GM, Hwang CR, Kang MJ, Shin JH (2014) Quality characteristics and biological activity of fermented black garlic with probiotics. J Life Sci, 24, 549-557 https://doi.org/10.5352/JLS.2014.24.5.549
  37. Ko EJ, Hur SS, Choi YH (1998) The establichment of optimum cultural conditions for manufacturing garlic vinegar. J Korean Soc Food Sci Nutr, 27, 102-108
  38. Woo CH, Park CH, Yoon HH (2000) Production of acetic acid from cellulosic biomass. Korean J Biotechnol Bioeng, 15, 458-463
  39. Yoon SR, Kim GR, Lee JH, Lee SW, Yeo SH, Jeong YJ, Kwon JH (2010) Properties of organic acids and volatile components in brown rice vinegar prepared using different yeasts and fermentation methods. Korean J Food Preserv, 17, 733-740
  40. Isuzugawa K, Ogihara Y, Inoue M (2001) Different generation of inhibitons against gallic acid induced apoptosis produces different sensitivity to gallic acid. Biol Pharm Bull, 24, 249-353 https://doi.org/10.1248/bpb.24.249
  41. Woo SM, Kim OM, Choi IW, Kim YS, Choi HD, Jeong YJ (2007) Condition of acetic acid fermentation and effect of oligosaccharide addition on kiwi vinegar. Korean J Food Preserv, 14, 100-104
  42. Hong SM, Moon HS, Lee JH, Lee HI, Jeong JH, Lee MK, Seo KI (2012) Development of functional vinegar by using cucumbers. J Korean Soc Food Sci Nutr, 41, 927-935 https://doi.org/10.3746/jkfn.2012.41.7.927
  43. Joo KH, Cho MH, Park KJ, Jeong SW, Lim JH (2009) Effects of fermentation method and brown rice content on quality characteristics of brown rice vinegar. Korean J Food Preserv, 16, 33-39