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Change of Aroma Compounds during Corn Vinegar Ripening

숙성에 의한 옥수수 식초의 향기 성분 변화

  • Shin, In-Ung (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Park, Eun-Hee (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University) ;
  • Choi, Yeong-Hwan (Kooksoondang Brewery Co. Ltd) ;
  • Ryu, Su-Jin (Kooksoondang Brewery Co. Ltd) ;
  • Shin, Woo-Chang (Kooksoondang Brewery Co. Ltd) ;
  • Kim, Myoung-Dong (Division of Food Biotechnology and Biosystems Engineering, Kangwon National University)
  • Received : 2019.02.07
  • Accepted : 2019.05.13
  • Published : 2020.03.28

Abstract

Vinegar was prepared from corn produced in Gangwon province and changes to the major aroma components were investigated for acetic acid fermentation and ripening. The most prevalent volatile component in corn alcohol was determined to be 2-phenylethanol (accounting for 27% of total aroma components); the levels of 2-phenylethanol decreased to 15% after acetic acid fermentation. Principal component analysis of volatile compounds revealed that corn vinegar was distinguishable from corn alcohol. The highest content of total volatile components (50%) was acetic acid, followed by 2-phenylethanol and diethyl succinate. Ethyl phenylacetate and diethyl succinate had the highest odor activity value, and might contribute most profoundly to the aroma of corn vinegar.

강원도에서 생산된 옥수수를 사용하여 식초를 제조한 후 초산 발효 및 숙성에 의해 변화되는 주요 향기성분을 조사하였다. 옥수수 술에서 가장 높은 함량을 나타낸 휘발성 성분은 2-phenylethanol으로서 전체 휘발성 성분의 약 27%를 차지하였으나, 초산 발효 후 15% 수준으로 감소하였다. 주성분 분석 결과 옥수수 술과 옥수수 식초는 초산 발효 및 숙성 기간에 따라 향기 성분의 특성이 패턴의 차이가 확인되었다. 초산 발효 이후에 전체 휘발성 물질 중에서 초산이 약 50%를 차지하여 가장 함량이 높은 것으로 나타났으며, 그 다음으로 2-phenylethanol, diethyl succinate 순서로 높았다. 장미향을 나타내는 ethyl phenylacetate와 과일향을 나타내는 diethyl succinate 등 에스테르 화합물의 향기 활성가가 높게 나타나 이들 성분이 초산 발효 후 식초의 향기 특성에 큰 영향을 미치는 것으로 판단되었다.

Keywords

References

  1. Yoon HN. 1999. Chemical characterization of commercial vinegars. Korean J. Food Sci. Technol. 31: 1440-1446.
  2. Park EH, Choi CY, Kwon HJ, Kim MD. 2016. Literature review on type and manufacturing methods of Korean traditional vinegar. Food Sci. Indust. 49: 94-99.
  3. Moon SY, Chung HC, Yoon HN. 1997. Comparative analysis of commercial vinegars in physicochemical properties, minor components and organoleptic tastes. Korean J. Food Sci. Technol. 29: 663-670.
  4. Park YO. 2018. Quality comparison of natural fermented vinegars manufactured with different raw materials. J. Korean Soc. Food Sci. Nutr. 47: 46-54. https://doi.org/10.3746/jkfn.2018.47.1.046
  5. Na HS, Choi GC, Yang SI, Lee JH, Cho JY, Ma SJ, et al. 2013. Comparison of characteristics in commercial fermented vinegars made with different ingredients. Korean J. Food Preserv. 20: 482-487. https://doi.org/10.11002/kjfp.2013.20.4.482
  6. Kwon SH, Jeong EJ, Lee GD, Jeong YJ. 2000. Preparation method of fruit vinegars by two stage fermentation and beverages including vinegar. Food Indust. Nutr. 5: 18-24.
  7. Oh YJ. 1992. A study on cultural conditions for acetic acid production employing pear juice. J. Korean Soc. Food Nutr. 21: 377-380.
  8. Park MH, Lee KO, Lee JY, Yu SJ, Ko YJ, Kim YH, et al. 2005. Isolation and characteristics of acetic acid bacteria for persimmon vinegar fermentation. J. Korean Soc. Food Sci. Nutr. 34: 1251-1257. https://doi.org/10.3746/jkfn.2005.34.8.1251
  9. Lee HY, Goh YJ, Park JS, Ahn MS, Kwon HJ. 2018. Optimization of acetic acid fermentation of hardy kiwi vinegar using low-pH tolerant Acetobacter pasterianus AFY-4. J. East Asian Soc. Dietaty Life. 28: 136-144. https://doi.org/10.17495/easdl.2018.4.28.2.136
  10. Kim ML, Choi KH. 2005. Sensory characteristics of citrus vinegar fermented by Gluconacetobacter hansenii CV1. Korean J. Food Cookery Sci. 21: 263-269.
  11. Lino T, Suzuki R, Tanaka N, Kosako Y, Ohkuma M, Komagata K, et al. 2012. Gluconacetobacter kakiaceti sp. nov., an acetic acid bacterium isolated from a traditional Japanese fruit vinegar. Inter. J. System. Evol. Microbiol. 61: 1465-1469.
  12. Jo YJ, Kim OM, Jeong YJ. 2013. Monitoring of the changes in volatile flavor components in oriental melon wine using SPME. Korean J. Food Presev. 20: 207-214. https://doi.org/10.11002/kjfp.2013.20.2.207
  13. Song H, Liu J. 2018. GC-O-MS technique and its applications in food flavor analysis. Food Res. Int. 114: 187-198. https://doi.org/10.1016/j.foodres.2018.07.037
  14. Noh BS. 2005. Analysis of volatile compounds using electronic nose and its application in food industry. Korean J. Food Sci. Technol. 37: 1048-1064.
  15. Zhu J, Chen F, Wang L, Niu Y, Chen H, Wang H, et al. 2016. Characterization of the key aroma volatile compounds in cranberry (vaccinium macrocarpon ait.) using gas chromatographyolfactometry (GC-O) and odor activity value (OAV). J. Agric. Food Chem. 64: 4990-4999. https://doi.org/10.1021/acs.jafc.6b01150
  16. Lee JK, Koo BS, Kim SY. 2003. Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis. Appl. Environ. Microbiol. 69: 6179-6188. https://doi.org/10.1128/AEM.69.10.6179-6188.2003
  17. Kim HJ. 2012. Application of metabolomics in food science. Food Sci. Indust. 45: 36-44.
  18. Roda A, Lucini L, Torchio F, Dordoni R, Faveri DM, Lambri M. 2017. Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste. Food Chem. 229: 734-742. https://doi.org/10.1016/j.foodchem.2017.02.111
  19. Joo KH, Cho MH, Park KJ, Jeong SW, Lim JH. 2009. Effects of fermentation methods and brown rice content on quality characteristics of brown rice vinegar. Korean J. Food Preserv. 16: 33-39.
  20. Choi CY, Park EH, Ryu SJ, Shin WC, Kim MD. 2018. Metabolome analysis and aroma characteristics of fermented fruit vinegar. Microbiol. Biotechnol. Lett. 46: 416-424. https://doi.org/10.4014/mbl.1806.06012
  21. Jo YH, Gu SY, Chung NH, Gao Y, Kim HJ, Jeong MH, et al. 2016. Comparative analysis of sensory profiles of commercial cider vinegars from Korea, China, Japan, and US by SPME/GC-MS, Enose, and E-tounge. Korean J. Food Sci. Technol. 48: 430-436. https://doi.org/10.9721/KJFST.2016.48.5.430
  22. Lee HD, Jeon SB, Choi WJ, Lee SS, Lee MH, Oh KJ. 2013. A novel assessment of odor sources using instrumental analysis combined with resident monitoring records for an industrial area in Korea. Atmospheric. Environ. 74: 277-290. https://doi.org/10.1016/j.atmosenv.2013.04.001
  23. Guth H. 1997. Quantitation and sensory studies of character impact odorants of different white wine varieties. J. Agric. Food Chem. 45: 3027-3032. https://doi.org/10.1021/jf970280a
  24. Welke JE, Zarus M, Lazzarotto M, Zini CA. 2014. Quantitative analysis of headspace volatile compounds using comprehensive two-dimensional gas chromatography and their contribution to the aroma of Chardonnay wine. Food Res. Int. 59: 85-99. https://doi.org/10.1016/j.foodres.2014.02.002
  25. Dragone G, Mussatto SI, Oliveira JM, Teixeira JA. 2009. Characterisation of volatile compounds in an alcoholic beverage produced by whey fermentation. Food Chem. 112: 929-935. https://doi.org/10.1016/j.foodchem.2008.07.005
  26. Acena L, Vera L, Guasch J, Busto O, Mestres M. 2011. Chemical characterization of commercial sherry vinegar aroma by headspace solid-phase microextraction and gas chromatographyolfactometry. J. Agric. Food Chem. 59: 4062-4070. https://doi.org/10.1021/jf104763u
  27. Butkhup L, Jeenphakdee M, Jorjong S, Samappito S, Samappito W, Chowtivannakul S. 2011. HS-SPME-GC-MS analysis of volatile aromatic compounds in alcohol related beverages made with mulberry fruits. Food Sci. Biotechnol. 20: 1021-1032. https://doi.org/10.1007/s10068-011-0140-4
  28. Zhu H, Zhu J, Wang L, Li Z. 2015. Development of a SPME-GC-MS method for the determination of volatile compounds in Shanxi aged vinegar and its analytical characterization by aroma wheel. J. Food. Sci. Technol. 53: 171-183.
  29. Duncan DB. 1955. Multiple range and multiple F test. Biometrics. 11: 1-42. https://doi.org/10.2307/3001478
  30. Ramadan Z, Jacobs D, Grigorov M, Kochhar S. 2006. Metabolic profiling using principal component analysis, discriminant partial least squares, and genetic algorithms. Talanta 68: 1683-1691. https://doi.org/10.1016/j.talanta.2005.08.042
  31. Worley B, Powers R. 2015. Multivariate analysis in metabolomics. Curr. Metabolomics 1: 92-107. https://doi.org/10.2174/2213235X11301010092
  32. Caja MM, Blanch GP, Herraiz M, del Castillo ML. 2004. On-line reversed-phase liquid chromatography-gas chromatography coupled to mass spectrometry for enantiomeric analysis of chiral compounds in fruit beverages. J. Chromatogr. 1054: 81-85. https://doi.org/10.1016/j.chroma.2004.04.050
  33. Aznar M, Lopez R, Cacho JF, Ferreira V. 2001. Identification and quantification of impact odorants of aged red wines from Rioja. GC-olfactometry, quantitative GC-MS, and odor evaluation of HPLC fractions. J. Agric. Food Chem. 46: 2924-2929.
  34. Hossain A, Rahman M. 2011. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Res. Int. 44: 672-676. https://doi.org/10.1016/j.foodres.2010.11.036
  35. Vogt M, Brusseler C, Ooyeon J, Bott M, Marienhagen J. 2016. Production of 2-mrthyl-1-butanol and 3-methyl-1-butanol in ehgineered Corynebacterium glutamicum. Metab. Eng. 38: 436-445. https://doi.org/10.1016/j.ymben.2016.10.007
  36. Kim HI, Hur YY, Jung SM, Im DJ, Chung KH, Kim SJ. 2018. Characteristics of aroma compounds of 11 red wines from international grape cultivars grown in Korea. Korean J. Food Preserv. 25: 491-500. https://doi.org/10.11002/kjfp.2018.25.5.491
  37. Lee SW, Yoon SR, Kim GR, Kyung HK, Jeong YJ, Yeo SH, et al. 2011. Effect of Nuruks and crude amylolytic enzyme on free amino acid and volatile components of brown rice vinegar prepared by static culture. Korean J. Food Sci. Technol. 43: 570-576. https://doi.org/10.9721/KJFST.2011.43.5.570
  38. Yoon SR, Kim GR, Lee SW, Jeong YJ, Yeo SH, Choi HS, et al. 2010. Volatile compounds and sensory properties of commercial brown rice vinegars fermented with and without ethanol. Korean J. Food Sci. Technol. 42: 527-532.
  39. Kim GR, Yoon SR, Lee JH, Yeo SH, Jeong YJ, Yoon KY, et al. 2010. Physicochemical properties of and volatile components in commercial fruit vinegars. Korean J. Food Preserv. 17: 616-624.
  40. Curran AM, Rabin SI, Prada PA, Furton KG. 2005. Comparion of the volatile organic compounds present in human odor using SPME-GC/MS. J. Chem. Econol. 31: 1607-1619. https://doi.org/10.1007/s10886-005-5801-4
  41. Chen T, Gui Q, Shi JJ, Zhang XY, Chen FS. 2013. Analysis of variation of main components during aging process of Shanxi aged vinegar. Acetic Acid Bact. 2: 31-38.
  42. Yoon HN, Moon SY, Song SH. 1998. Volatile compounds and sensory odor properties of commercial vinegars. Korean J. Food Sci. Technol. 30: 299-305.