• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.697-700
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• 2001

Iron is an essential nutrient for most organisms, which supplied to them in a protein-iron complex known as ferritin. Ferritins are multimeric proteins found in prokaryotes, plants and animals. They are consisted of spherical shell of 24 subunits defining a cavity of about 8nm in diameter, where an iron core is laid down. Expression of ferritin in recombinant E. coli at $37^{\circ}C$ led to the accumulation of recombinant ferritin. Insoluble form of ferritin was separated from disrupted cells, followed by various primary separation steps with two kinds of buffers. Collected samples from the primary steps were purified by DEAE-cellulose gels packed in a column. The fractions from the DEAE column were assayed to gain the amount and the purity of ferritin by using HPLC and SDS-PAGE.

• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.156-163
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• 2001

Vanadium has been known as environmental polluants resulted from the burning of fossil fuels in nature. It led to toxic responses by prooxidant activity, inducing free radicals and the accumulation in the tissues. Recently, there has been growing interest in an essential nutritional requirement of vandium and especially the treatment of diabetes. But because of its strong toxicity, thease chemicals have narrow safety margin. In order to reduce metal toxicity, and increase absorption and biological activities, metal ions such as selenium and chromium were uptaken in yeast cells. In this study, Vanadium yeast was prepared by uptaking vanadate in yeast cells. Vanadate induced hematological and biochemical changes in the experimental rat blood were inhibited by the treatments of vanadium yeast. Lipid peroxidation and catalase activity were significantly increased in kidney and liver after a single intraperitoneal injection of vanadate to rats. However, these observations were apparently reduced in the vanadium yeast treated group. Vanadium amount in blood, kidney and liver after a single intraperitoneal injection of vanadium yeast was significantly reduced than that of vanadate treated group. In conclusion, vanadium yeast uptaken vanadate in yeast cells could reduce toxic effects of vanadate.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2002년도 춘계학술대회 논문집
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• pp.134-139
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• 2002

Motorcycle industry, for its competitive position, depends on part design technology. This part design has a bulky amount of data and asks the accumulation of advanced technical skill and experience, and fragmentary technical application is not enough to get to the kernel of a problem. Therefore, the improvement of proper productivity - the starting point of engineering design - and useful Design Methodology for products manufacturing are needed. Thus this paper is aimed to create a program that outputs dynamic characteristics of a vehicle when the data from fully understanding on motorcycle's brake system and induced formula for brake design are inputted.

• 한국유기농업학회지
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• 제2권1호
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• pp.2-15
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• 1993

Major vegetables and fruits in Korea are grouped in 3 Categories according to the Concentration of NO3- content in edible parts of the plant. NO3- content are high in order fruit

• 한국공작기계학회논문집
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• 제12권1호
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• pp.101-110
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• 2003

Motorcycle industry, for its competitive position, depends on part design technology. This part design has a bulky amount of data and asks the accumulation of advanced technical skill and experience, and fragmentary technical application is not enough to get to the kernel of a problem. Therefore, the improvement of proper productivity - the starting point of engineering design - and useful Design Methodology for products manufacturing are needed. Thus this paper is aimed to create a program that outputs dynamic characteristics of a vehicle when the data from fully understanding on motorcycle's brake system and induced formula for brake design are inputted. This paper is intended to show a creative design method by the thermal analysis data through FEM study and using shape design parameters derived from our programs.

• 미생물학회지
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• 제20권4호
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• pp.195-200
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• 1982

Rhizobium japonicum was isolated from the nodule of soybean root grown at the reclaimed tidal land in Kang-Wha island. The effect of pH and salt concentration to the viability of the isolated strain were examined in relationship between microbial growth and accumulation of PHB. Optimal pH value for the good viability of the isolated strain was 7.0 and also, at 5.0 and 6.0 viability was favorable to large extent, but 9.0 was unfavorable. Examined the effect of salt concentration treated two times as of the salinity in the reclaimed tidal land, viability of the isolated strain showed about 30 to 40%. And also in treatment with NaCl(40g/l) whatever the pH value adopted, viability was mostly less than 10%. The amount of accumulated PHB was relatively high at low pH value(5-6) and at high salt concentrration, respectively.

• 기술경영경제학회:학술대회논문집
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• 기술경영경제학회 2003년도 제22회 동계학술발표회 논문집
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• pp.27-40
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• 2003

Though it has been recognized that the accumulation of technological knowledge has been the core competency to reinforce the competitiveness of individual firms and to raise the innovation capability of social and economic systems, only single or fragmentary variables, such as R&D expenditure, R&D stock, the number of researchers and the number of R&D employee have been adopted to measure the amount of technological knowledge. In this research, we use nine conventional technological knowledge measures under the conceptual structure of input-output framework to technological knowledge. Applying correlation and factor analysis, we examine the relationships among the nine proxy measures quantitatively and suggest the new approach for the calculation of technological knowledge index as a aggregated measure.

• Asian Journal of Atmospheric Environment
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• 제7권3호
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• pp.161-168
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• 2013

In this study, an investigation was conducted to assess the washout mechanism of Asian dust particles through both laboratory-scale experiment and model calculation. To artificially simulate Asian dust particle, $CaCO_3$ particles were generated inside an experimental chamber. They were then scavenged by the artificial rain drops. The abundant $CaCO_3$ particles scavenged on a rain drop were successively identified by SEM observation. The concentrations of Ca in residual $CaCO_3$ particles on individual droplet were quantified by PIXE analysis. There was a tendency toward a high accumulation of Ca on a relatively small drop (e.g., <1.0 mm diameter). It is thus suggested that smaller rain drops can effectively scavenge a significant amount of Asian dust particles in ambient atmosphere. The numerical estimation can account for 92.1% and 83.2% of Ca that were measured in small (<1.0 mm diameter) and large (>2.0 mm diameter) size drops, respectively.

• Environmental Engineering Research
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• 제20권1호
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• pp.25-32
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• 2015

The growth of the algae strains Neochloris oleabundans, Botryococcus Braunii and Dunaliella sp. under mixotrophic conditions in the presence of different concentrations of crude glycerol was evaluated with the objective of increasing the biomass growth and algal oil content. A high biomass concentration was characteristic of these strains when grown on crude glycerol compared to autotrophic growth, and 5 g/L glycerol yielded the highest biomass concentration for these strains. Mixotrophic conditions improved both the growth of the microalgae and the accumulation of triacylglycerols (TAGs). The maximum amount of TAGs in the algal strains was obtained in the 5 g/L glycerol growth medium. The fatty acid profiles of the oil for the cultures met the necessary requirements and are promising resources for biofuel production.

• 한국미생물·생명공학회지
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• 제21권2호
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• pp.119-124
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• 1993

An alkalophilic microorganism for overproduction of cyclodextrin glucanotransferase (CGTase) was newly isolated from hot-water spring soil, and identified as Bacillus firmus var. alkalophilus H609. The strain maintained stability during preservation and cultivation for the enzyme production, and produced significant amount of CGTase corresponding to the volumetric activity of 75 units/mL at 37C, initial pH of 11.2, and after 40 hours. The strain excreted several different proteins showing CGTase activity that catalyzed the formation of mainly beta-and Gamma-type cyclodextrin (ratio of 7:1) from soluble starch without accumulation of alpha-type. Other enzymatic properties were also investigated.