• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.230-235
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• 2004

We previously reported that the phytoprotein extracted from Acanthopanax senticosus (PA) had anti-carcinogenic anti-metastatic activity via increase of inhibition of gap junctional intercellular communication. In the present study investigated the immunomodulatory mechanism of phytoprotein isolated from the stem bark of Acanthopanax sentic (PA). PA was found to significantly stimulate macrophages producing TNF-α and IL-1β in vitro. It also showed tumori activity indicating that PA had the ability to stimulate macrophage directly. Moreover, PA induced the CDB/sup +/ CTL cy responses to recognize antigen on the B16-BL6 melanoma cells. Treatment of PA with B16-BL6 melanoma cells increased the proliferation of splenocytes compared with untreated control. These results demonstrate that PA immunomodulatory activity suggesting a useful anti-tumor agent applicable to treatment and prevention of cancer.

• The Journal of Internal Korean Medicine
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• v.43 no.4
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• pp.529-541
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• 2022

Objectives: Acanthopanax senticosus is a tree used in traditional medicine for various diseases. In this study, we investigated the anti-cancer effects of a water extract of Acanthopanax senticocus fruit (ASF) on 2 human breast cancer cell lines (MCF-7 and MDA-MB-231). Methods: The MTT assay was used to assess cell proliferation. The expression of apoptosis-related genes was assessed by quantitative real-time PCR. Results: ASF treatment caused a dose-dependent inhibition of cell growth in both estrogen-independent MDA-MB-231 and estrogen-dependent MCF-7 breast cancer cells. ASF decreased mRNA expression of the apoptotic suppressor gene Bcl-xL, and increased mRNA expression of proapoptotic genes. ASF increased the mRNA expression of p21 and RIP-1 in both cell types. ASF decreased the mRNA expression of survivin in the MCF-7 cell line. Conclusions: ASF exhibits anti-cancer activity involving apoptotic cell death.

• Korean Journal of Medicinal Crop Science
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• v.6 no.2
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• pp.114-120
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• 1998

The present study was carried out to investigate the changes in essential oil contents as a part of searching the changes in active components in the graft cultivation of A. senticosus. To increase the yield of aerial parts and the contents of active components of Acanthopanax senticosus, scions of A. senticosus were grafted on different rootstocks. As a result, the contents of calarene, ${\alpha}-beramotene$ and spathulenol increased, but cis-caryophyllene and epizonarene contents decreased. ${\beta}-pinene$, ${\beta}-myrcene$, 2,5,5 -trimethyl-1, 3, 6-heptatriene and ${\beta}-elemene$ were not detected in the leaves of grafted A. senticosus. Essential oils from stems did not differ with the methods of cultivation. Oil contents of 3-year-old plants were higher than those of 1-year-old plants, whereas ${\alpha}-bergamotene$ content(27%) was highest in the stem of grafted A. senticosus(27%). Ethylbenzene, 2-furancarboxaldehyde, M134 $(t_R=2.11)$ and M205 $(t_R=3.16)$ disappeared in the roots of A. senticosus var. subinermis, but 6,6-dimethyl-3-methylenebicycloheptane, M166 $(t_R=1.82)$, and (+) -aromadendrene and ${\beta}-bisabolene$ were newly found.

• Natural Product Sciences
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• v.9 no.2
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• pp.45-48
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• 2003

The content of chiisanoside in the Acanthopanax Species was determined by reversed-phase high performance liquid chromatographic method. Chiisanoside was separated from the other components in the plant extracts using Zorbax 300 SB $C_{18}$ column with gradient elution of acetonitrile. Identification of chiisanoside was carried out by comparison in the LC/MS spectrum of separated peak from extract with that of standard. By HPLC analysis in this experiment, Acanthopanax species could be classified into two groups based upon the content of chiisanoside-one with low concentration of chiisanoside, such as A. senticosus and A. koreanum, and another with high concentration of chiisanoside, such as A. senticosus f. inermis, A. Divaricatus var. albeofructus, and A. chiisanensis.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2001.06a
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• pp.89-92
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• 2001

오갈피속 수종은 우리나라에 10여 종 있는 것으로 보고되고 있는데(김영진, 등 1997)약용성분이 가장 뛰어난 수종은 가시오갈피(Acanthopanax senticosus (Ruper. et Max.)Harms)로 알려져 있다. 가시오갈피는 높이 2∼3 m의 낙엽활엽관목으로서 강한 햇볕이 드는 더운곳에서는 거의 발견되지 않으며 내음 및 내한성이 강하다.(중략)

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.60.1-60.1
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• 2007

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• Journal of Acupuncture Research
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• v.19 no.2
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• pp.1-13
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• 2002

Objective ; In order to study the effect of Acanthopanax senticosus(AS) aqua-acupuncture manufactured with water soluble fraction and ether soluble fraction on the streptozotocin induced diabetic rats Methods ; The fractions of AS aqua-acupuncture were carried out on corresponding bilateral loci of Bisu(BL20) everyday for 4 weeks. The experimental animals were divided into control group and AS groups(AS water fraction group and AS ether fraction group). Thereafter the levels of serum glucose, total choleterol, HDL, triglyceride, AST, ALT, creatinine, BUN, urinary albumin excretion, index of kidney hypertrophy, heart rate, mean blood pressure and fibronectin in glomeruli and tubular cells were measured. Results ; The increased serum total cholestrol, triglyceride levels, HDL and urinary albumin excretion, the index of kidney hypertrophy, the mean blood pressure and the amount of fibronectin in glomeruli and tubular cells were significantly decreased in the AS groups, showing more significant decrease in the AS water fraction group as compared with the control group. In the serum ALT, AST, creatinine and BUN levels, there were no significant changes in the AS groups as compared with the control group. Conclusion ; According to the above results, it reveals that Acanthopanax senticosus water soluble and ether soluble fraction have the antidiabetic effect, the antilipidemic effect and the inhibitory effect of renal damage. Also, the results showed that Acanthopanax senticosus water soluble fraction is more effective than ether soluble fraction.

• Korean Journal of Agricultural Science
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• v.35 no.1
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• pp.11-17
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• 2008

This study was conducted to isolate polyacetylenes from the Acanthopanax senticosus and to identify the chemical structure of the polyacetylenes by UV, IR, $^1H$-NMR and $^{13}C$-NMR. One of the liposoluble materials was extracted with petroleum ether. Polyacetylene compounds were collected through solvent fractionation at silica gel column chromatograph. The HPLC was used for the semi-preparative separation IR spectra of fraction 5 showed triple bonds at $2256cm^{-1}$ and double bond at $1654cm^{-1}$, respectively, $^1H$-NMR spectra of Fraction 5 showed the double bond at 5.35-5.48 ppm. Triple bond at 64.0. 71.2, 74.2, 80.2 ppm and double bond at 121.89, 133.0 ppm were observed in the $^{13}C$-NMR spectra.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.1
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• pp.40-46
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• 2014

We demonstrated that Acanthopanax senticosus fermentation products (FM-5111 and FM-5131) administered to rats functionally protect against DL-ethionine-induced and ethanol-induced fatty liver models. In DL-ethionine-induced fatty liver models, the serum concentrations of aspartate aminotransferase (AST), as well as liver concentrations of triglyceride and total lipid against the control decreased in FM-5111 and FM-5131 treated rats. In ethanol-induced fatty liver models, FM-5111 and FM-5131 treated rats showed a decrease in the liver concentrations of triglyceride and total lipid in ethanol-induced fatty liver models. There were no significant differences in the serum concentrations of AST and alanine aminotransferase in FM-5111 and FM-5131 treated rats. Additionally, FM-5111-, or FM-5131-treated rats showed no significant differences in the body weight gain between the control. These results indicate that Acanthopanax senticosus fermentation products might have protective effects against DL-ethionine-induced and ethanol-induced fatty liver models.

• Journal of Life Science
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• v.22 no.12
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• pp.1704-1711
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• 2012

Three mold strains, Aspergillus oryzae (A. oryzae), Aspergillus kawachii (A. kawachii), and Monascus purpureus (M. purpureus), were tested for fermentation of Acanthopanax senticosus (A. senticosus) leaf, root, stem, and fruit powders. The fermented A. senticosus materials were then tested for bioactive materials (phenolic compounds, flavonoids, mineral and fatty acid) and biological activities (DPPH free radical scavenging activity, reducing power, and tyrosinase inhibition activity). The highest concentrations of phenolic compounds and flavonoids were NFASL at 4.11% and MPASL at 2.30%, respectively. Major minerals were Ca, K, Mg and Mn. Major fatty acids in fermented A. senticosus powders were palmitic, linolenic, and stearic acids. DPPH radical scavenging activity was slightly stronger in non-fermented than in fermented A. senticosus. Tyrosinase inhibition activity was stronger in fermented A. senticosus than in NFAG. The Fe/Cu reducing powers were stronger in non-fermented A. senticosus than in any of the fermented A. senticosus materials. Overall, the study provides basic data for understanding the biological activities and chemical characteristics of A. senticosus fermented by molds for the development of functional foods.