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Determination of Chiisanoside in Acanthopanax Species by High Performance Liquid Chromatography  

Kang, Jong-Seong (College of Pharmacy, Chungnam National University)
Linh, Pham Tuan (College of Pharmacy, Chungnam National University)
Cai, Xing Fu (College of Pharmacy, Chungnam National University)
Lee, Jung-Joon (Korea Research Institute of Bioscience and Biotechnology)
Kim, Young-Ho (College of Pharmacy, Chungnam National University)
Publication Information
Natural Product Sciences / v.9, no.2, 2003 , pp. 45-48 More about this Journal
Abstract
The content of chiisanoside in the Acanthopanax Species was determined by reversed-phase high performance liquid chromatographic method. Chiisanoside was separated from the other components in the plant extracts using Zorbax 300 SB $C_{18}$ column with gradient elution of acetonitrile. Identification of chiisanoside was carried out by comparison in the LC/MS spectrum of separated peak from extract with that of standard. By HPLC analysis in this experiment, Acanthopanax species could be classified into two groups based upon the content of chiisanoside-one with low concentration of chiisanoside, such as A. senticosus and A. koreanum, and another with high concentration of chiisanoside, such as A. senticosus f. inermis, A. Divaricatus var. albeofructus, and A. chiisanensis.
Keywords
Acanthopanax species; chiisanoside; HPLC; LC-MS;
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1 Bae, E. A., Yook, C. S., Oh, O. J., Chang, S. Y. Nohara, T., Kim, D. H., Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Bioi. Pharrn. Bull. 24, 582-585 (2001)   DOI   ScienceOn
2 Park, S. Y., Chang, S. Y., Yook, C. S., Nohara, T., New 3,4-seco-lupane-type triterpene glycosides from Acanthopanax senticosus forma inermis. J. Nat. Prod. 63, 1630-1633 (2000)   DOI   ScienceOn
3 Oh, O. J., Chang, S. Y., Yook, C. S., Yang K. S., Park, S. Y., Nohara, T., Two 3,4-seco-Iupane triterpenes from leaves of Acanthopanax divaricatus var. albeofructus. Chern. Pharrn. Bull. 48, 879-881 (2000)   DOI   ScienceOn
4 Kim, C. J., Hahn, D. R., The biological activity of a new glycoside, chiisanoside from Acanthopanax chiisanensis Nakai leaves. Yakhak Hoeji 24, 123-134 (1980)
5 Yook, C. S., Rho, Y. S., Seo, S. H., Leem, J. Y., Han, D. R., Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40, 251-261 (1996)
6 Kang, J. S., Pham, T. L., Cai, X. F.,Kim, H. S., Lee J. J., Kim, Y. H., Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography. Arch. Pharm. Res., 24,407-411 (2001)   DOI   ScienceOn
7 Kim, Y. O., Cho, D. H., Chung H. J., Kim, J. H., Chang, S. Y., Yook, C. S., Yang, K. S., Oh, O. J., Effects of lupane-triterpenoids on mitogen-induced proliferation of lymphocytes. Yakhak Hoeji 43, 208-239 (1999)
8 Hahn D. R., Kasai R., Kim J. H., Taniyasu S., and Tanaka O., A new glycosyl ester ofa 3,4-seco-triterpene from Korean medicinal plant, Acanthopanax chiisanensis. Chern. Pharrn. Bull. 32, 1244-1247 (1984)   DOI
9 Kim, Y. H., Chung, B. S. Sankawa, U., Pimaradiene diterpenes from Acanthopanax koreanurn. J. Nat. Prod., 51, 1080~1083 (1988)   DOI
10 Perry, L. M., and Metzger, J., Medicinal plants of east and southeast of Asia. MIT Press, Cambridge, MA and London, 1980, pp. 41.
11 Yook, C. S., Medicinal herbs of Acanthopanax in Asia. Kyeongwon Media, Seoul, 2001
12 Nishibe S, Kinoshita H, Takeda H, Okano G., Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats. Chern. Pharrn. Bull. 38, 1763-1765 (1990)   DOI   ScienceOn
13 Cho, H. K, Ham, I., Whang, W. K, Constituents and quantitative analysis from the leaves of Acanthopanax divaricatus f. narnbunensis. Yakhak Hoeji 43, 294-299 (1999)
14 Park, S. Y. Studies on RAPD analysis and triterpenoidal constituents of Acanthopanax species. PH. D. Dissertation, Kumamoto University (2002)