• Molecules and Cells
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• v.41 no.3
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• pp.234-243
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• 2018

In recent years, the interest towards the relationship between incretins and bone has been increasing. Previous studies have suggested that glucagon-like peptide-1 (GLP-1) and its receptor agonists exert beneficial anabolic influence on skeletal metabolism, such as promoting proliferation and differentiation of osteoblasts via entero-osseous-axis. However, little is known regarding the effects of GLP-1 on osteoblast apoptosis and the underlying mechanisms involved. Thus, in the present study, we investigated the effects of liraglutide, a glucagon-like peptide-1 receptor agonist, on apoptosis of murine MC3T3-E1 osteoblastic cells. We confirmed the presence of GLP-1 receptor (GLP-1R) in MC3T3-E1 cells. Our data demonstrated that liraglutide inhibited the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation, as detected by Annexin V/PI and Hoechst 33258 staining and ELISA assays. Moreover, liraglutide upregulated Bcl-2 expression and downregulated Bax expression and caspase-3 activity at intermediate concentration (100 nM) for maximum effect. Further study suggested that liraglutide stimulated the phosphorylation of AKT and enhanced cAMP level, along with decreased phosphorylation of $GSK3{\beta}$, increased ${\beta}-catenin$ phosphorylation at Ser675 site and upregulated nuclear ${\beta}-catenin$ content and transcriptional activity. Pretreatment of cells with the PI3K inhibitor LY294002, PKA inhibitor H89, and siRNAs GLP-1R, ${\beta}-catenin$ abrogated the liraglutide-induced activation of cAMP, AKT, ${\beta}-catenin$, respectively. In conclusion, these findings illustrate that activation of GLP-1 receptor by liraglutide inhibits the apoptosis of osteoblastic MC3T3-E1 cells induced by serum deprivation through $cAMP/PKA/{\beta}-catenin$ and $PI3K/Akt/GSK3{\beta}$ signaling pathways.

• Food Science of Animal Resources
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• v.35 no.3
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• pp.277-285
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• 2015

This study was conducted to investigate the effects of kimchi powder and onion peel extract on the quality characteristics of emulsion sausage manufactured with irradiated pork. The emulsion sausages were formulated with 2% kimchi powder and/or 0.05% onion peel extract. The changes in pH value of all treatments were similar, depending on storage periods. The addition of kimchi powder increased the redness and yellowness of the emulsion sausage. The addition of onion peel extract decreased the thiobarbituric acid reactive substances value of the emulsion sausages prepared with irradiated pork. The volatile basic nitrogen value of the emulsion sausage prepared with kimchi powder was the highest, whereas that of the emulsion sausage prepared with onion peel extract was the lowest. The treatment without kimchi powder or onion peel extract and the treatments prepared with onion peel extract showed lower microbial populations than the other treatment. Sensory evaluations indicated that a higher acceptability was attained when kimchi powder was added to the emulsion sausages manufactured with irradiated pork. In conclusion, our results suggest that combined use of kimchi powder and onion peel extract could improve quality characteristics and shelf stability of the emulsion sausage formulated with irradiated pork during chilled storage.

• Applied Chemistry for Engineering
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• v.3 no.1
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• pp.148-155
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• 1992

For the enhancement of the concrete durability, we developed high - durable portland cement (ACTPC) by physical treatment of ordinary portland cement with asphalt and carbon black. In this research, the hydration reaction characteristics of ACTPC were studied by using SEM, X-Ray, Conduction Micro Calolimeter etc. When the ACTPC is mixed with water, it is believed the asphalt films on cement particles suppress the early stage of hydration reactions of cements, because the films hinder the contact with water and the elusion of soluble ions from particles. But the carbon blacks on the cement particles accelerated the hydration reactions of cement after a while, because the micro particles can be used as seed crystals in $CaO-SiO_2-H_2O$ gel formation.

• Korean Journal of Veterinary Pathology
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• v.1 no.1
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• pp.1-6
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• 1997

The cellularity and composition of the spleen lymph node thymus and peripheral blood and tempo of regeneration were studied at various time points after syngeneic bone marrow transplantation(BMT) in C3H/Hen mice. Significant depression of absolute lymphocyte count was noted on week 1 after lethal whole-body irradiation and BMT. In comparison to the lymph node thymus and spleen had an rapid regeneration of cellularity. The distinct cell populations($CD4^+,\;CD8^+,\;CD28^+,\;B220^+) have determined in the lymphoid tissue of mice subjected to irradiation. The relative representation of these subpopulations was significantly different from that in nonirradiated control. $CD4^+\;and\;CD8^+$ cells were present in very low numbers whereas the $B220^+$ cells reached more than normal range at 2 weeks after BMT. The number of $CD4^+$ cells returned to normal relatively soon than $CD8^+$ cell. At week 4 after BMT, the cellularity and composition of spleen lymph node and peripheral blood lymphocyte reached about 50% of the normal range therefore we can choose this time point for the other tests of immune function after BMT.

• Proceedings of the Korea Water Resources Association Conference
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• 2011.05a
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• pp.202-206
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• 2011

본 연구에서는 정체성 수역인 신갈호를 대상으로 저층 퇴적물의 오염물질에 대한 조사를 실시 하였다. 신갈호 저층 퇴적물의 분석 결과, 함수율의 범위는 49.0~68.2%로 조사되었으며, 평균 함수율은 60.8%로 나타났다. 함수비의 경우에는 지점별 96.1~214.6%의 넓은 범위를 보였으며 평균 함수비는 165.8%로 조사되었다. 퇴적물의 pH는 모든 지점에서 산성을 띄었으며, 하류부로 갈수록 더욱 낮아지는 경향을 보였다. 입도분석 결과, 토성은 SL(사양토) 및 SiL(실트질양토)로 나타났으며, 이러한 토성분포는 입자별 침강속도에 의한 것으로 퇴적물의 지점별 이 화학적 특성에 많은 영향을 줄 것으로 판단된다. 유기물 함량 분석결과, 강열감량의 경우 8.22~11.36%로 평균 10.22% 의 유기물 함량을 보였으며, COD는 24,92 mg/kg~27,38 mg/kg의 값으로 평균 26,16 mg/kg로 조사되었다. 영양물질 함량 분석결과, T-N의 경우 신갈호 저층 퇴적물 평균 2,916 mg/kg의 질소 함량을 보였으며, T-P의는 평균 710 mg/kg로 조사되었다. 영양물질 함량 분석결과 하류부로 갈수록 높아지는 경향을 보였으나 총질소의 경우 지점별 비슷한 값을 보인 반면, 총인은 지점별 큰 차이를 보였다. 퇴적물의 중금속 함량은 Cd, Cu, Zn, $Cr^{6+}$, Pb을 분석하였으며, Cd 0.10~0.12 mg/kg, Cu 18.33~20.67 mg/kg, Zn 82.73~110.15 mg/kg, $Cr^{6+}$ 0.78~0.93 mg/kg Pb 11.04~14.53 mg/kg의 범위를 보였으며, 지점별로 큰 차이를 보이지 않았다. 본 연구의 자료는 신갈호 유역의 개발계획 및 준설 검토에 대한 유용한 자료로 활용 가능할 것으로 판단된다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.3 no.3
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• pp.165-169
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• 2002

Silicon wafer is very important accuracy make use semiconductor device substrate. In this research, for the uniformity dopant density distribution obtained to Neutron Transmutation Doping on make use Si in P Doping study work. In this research. we irradiated neutron on FZ silicon wafers which had high resistivity (1000~2000 ${\Omega}$cm), HANARO reactor was utilized resistivity changes due to observed, the generation of neutron irradiation on point defect analyzed, point defect on resistivity changes inquire into the effect. Before neutron irradiation theoretical due to calculated 5 ${\Omega}$-cm, 20.1 ${\Omega}$-cm for HTS hole and 5 ${\Omega}$-cm, 26.5 ${\Omega}$-cm, 32.5 ${\Omega}$-cm for IP3 hole. After neutron irradiation through SRP measurement the designed resistivities were approached, which were 2.1 H-cm for HTS-1, 7.21 ${\Omega}$-cm for HTS-2, 1.79 ${\Omega}$-cm for IP-1, 6.83 ${\Omega}$-cm for IP-2, 9.23 ${\Omega}$-cm for IP-3, respectively. Also after neutron irradiation resistivity changes due to thermal neutron dependent irradiation hole types free.

• Journal of Adhesion and Interface
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• v.15 no.4
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• pp.145-150
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• 2014

Dicyclopentadiene (DCPD)-based hydrocarbon resins are widely used as tackifiers in many applications. In particular, hydrogenated DCPD-based hydrocarbon resins are widely used in premium hot-melt-type adhesives such as hot melt adhesives (HMAs) and/or hot melt pressure-sensitive adhesives (HMPSAs), because are water-white in color and possess excellent stability to light and heat. This article discusses the adhesive performance of various hydrogenated DCPD resins when they are used as tackifiers in styrene-block-copolymer (SBC)-based HMPSAs. This article shows the correlation between the characteristics of tackifiers and the adhesive performance of SBC-based HMPSAs. The higher the softening point of the tackifier, the higher is the $T_g$, softening point, and crossover temperature of the PSAs. High aromatic H wt% content reduces the high-temperature resistance of PSAs, as suggested by the decrease in the crossover temperature and softening point of the PSAs.

• Bulletin of the Korean Chemical Society
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• v.29 no.5
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• pp.1038-1042
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• 2008

Mono and multilayer TiO2(Fe, $PEG_{600}$) films were deposited by the dip-coating on $SiO_2$/glass substrate using sol-gel method. In an attempt to improve the antibacterial properties of doped $TiO_2$ films, the influence of the iron oxides and polyethilenglycol ($PEG_{600}$) on the morphological, optical, surface chemical composition and biological properties of nanostructured layers was studied. Complementary measurements were performed including Spectroscopic Ellipsometry (SE), Scanning Electron Microscopy (SEM) coupled with the fractal analysis, X-Ray Photoelectron Spectroscopy (XPS) and antibacterial tests. It was found that different concentrations of Fe and $PEG_{600}$ added to coating solution strongly influence the porosity and morphology at nanometric scale related to fractal behaviour and the elemental and chemical states of the surfaces as well. The thermal treatment under oxidative atmosphere leads to films densification and oxides phase stabilization. The antibacterial activity of coatings against Escherichia Coli bacteria was examined by specific antibacterial tests.

• Journal of the Korean Ceramic Society
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• v.51 no.2
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• pp.88-96
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• 2014

Natural hydraulic lime (NHL) is produced by burning a form of low-grade limestone containing silica and alumina which, above certain temperatures, combine with calcium oxide. The resulting silicates and aluminates impart hydraulic properties to the product. This study aims to determine the calcined characteristics of NHL using domestic low-grade limestone with maximized hydraulic properties. Six types of low-grade limestone containing $SiO_2$ were selected and experiments were carried out with different burning temperatures and holding times. The burning temperature and holding time as the most suitable burning conditions were $1,200^{\circ}C$ to $1,300^{\circ}C$ and 3 to 7 h, respectively, for the manufacturing of NHL from domestic low-grade limestone. These results demonstrate the feasibility of NHL using domestic low-grade limestone to produce NHL.

• Journal of the Korean Vacuum Society
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• v.18 no.1
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• pp.73-78
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• 2009

Zinc Oxide (ZnO) nanorods arrays were deposited on ZnO buffered p-Si(100) substrates by hydrothermal method. The ZnO buffer layer with a thickness of 30 nm was deposited by metal oxide chemical vapor deposition at $500^{\circ}C$. The structural and optical properties of ZnO nanorods arrays controlled by precursor concentrations from 0.06 to 0.5 M were studied by FE-SEM(field emission scanning electron microscopy), XRD(X-ray diffraction), and PL(photoluminescence), respectively. It was found that the structural and optical properties of ZnO nanorods arrays are changed significantly with increase of precursor concentration. The sizes of diameter and length of nanorods were increased as the concentration increase, and good optical property was shown with the concentration of 0.3 M.