Korean Journal of Veterinary Pathology (한국수의병리학회지)

Korean Society of Veterinary Pathology (한국수의병리학회)
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Regeneration of Immune System after Syngeneic Bone Marrow Transplantation in Irradiated Mice: I. The Changes of Cell Number in Peripheral blood and Lymphatic Organs and Composition of Lymphocyte Subsets

마우스 동계골수이식 후 면역체계의 재생: I. 말초혈액 및 림프장기 세포의 숫적 변 화와 림프구아군의 분포

  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Heon (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Song-Eun (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Soon-Tae (Kyungpook Veterinary Service Laboratory) ;
  • Jo, Sung-Kee (Korea Atomic Energy Research Institute) ;
  • Hyun, Byung-Hwa (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ryu, Si-Yun (College of Veterinary Medicine, Chungnam National University) ;
  • Daynes, Raymond A. (School of Medicine, University of Utah)
  • Published : 1997.04.01
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Abstract

The cellularity and composition of the spleen lymph node thymus and peripheral blood and tempo of regeneration were studied at various time points after syngeneic bone marrow transplantation(BMT) in C3H/Hen mice. Significant depression of absolute lymphocyte count was noted on week 1 after lethal whole-body irradiation and BMT. In comparison to the lymph node thymus and spleen had an rapid regeneration of cellularity. The distinct cell populations($CD4^+,\;CD8^+,\;CD28^+,\;B220^+) have determined in the lymphoid tissue of mice subjected to irradiation. The relative representation of these subpopulations was significantly different from that in nonirradiated control. $CD4^+\;and\;CD8^+$ cells were present in very low numbers whereas the $B220^+$ cells reached more than normal range at 2 weeks after BMT. The number of $CD4^+$ cells returned to normal relatively soon than $CD8^+$ cell. At week 4 after BMT, the cellularity and composition of spleen lymph node and peripheral blood lymphocyte reached about 50% of the normal range therefore we can choose this time point for the other tests of immune function after BMT.

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