• 제목/요약/키워드: Zoysia grass

검색결과 76건 처리시간 0.021초

추비의 시용이 한국잔디(Zoyisa japonica Steud.)의 춘계생육에 미치는 영향 (Effect of the Application of Fertilizer in Autumn on the Development of Korean Lawn grass( Zoysia japonica Steud.) in the Following Spring)

  • 이정재;김인철;함선규;김성태;양승원
    • 아시안잔디학회지
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    • 제6권1호
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    • pp.39-45
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    • 1992
  • This experiment was undertaken to study effect of fall Fertitizarion on spring regrowth of Korean lawngrass( Zoysia japonica Steud ) Results obtained are summarized as follows : 1. late fall fertilization stimulated the spring regrowth of Korean lawngrass. hut early spring application showed little effect on that 2. The yield of clippings on May 21 was obviously greater in late fertilization plots(treatment C, D and E) than other plots(treatment A, B and control, but there was no significant difference in clipping yields of C, D and F treatment. 3. Both nitrogen and reducing sugar contents of runner fell down in spring. It was suggested thatthese constituents be translocated from runner to other parts during the budding period. 4. The nitrogen content of runner was obviously mote in the plot of late fall fertilization. In contrast to nitrogen, no obvious difference was found in reducing sugar content of runner of seven application date. It was suggested that the roots of plant be still able to absorb to absorb nitrogen under dormancy. .5.From these results. late fall fertilization enhances the development of Korean lawngrass in the following spring. But early spring application shows little effect on spring regrowth.

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Production of a Phytotoxic Compound, 3-Phenylpropionic Acid by a Bacterial Endophyte, Arthrobacter humicola YC6002 Isolated from the Root of Zoysia japonica

  • Chung, Eu-Jin;Park, Joo-Hwang;Park, Tae-Soon;Ahn, Jong-Woong;Chung, Young-Ryun
    • The Plant Pathology Journal
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    • 제26권3호
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    • pp.245-252
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    • 2010
  • An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

관악산의 잔디와 억새 생태계에 있어서 에너지의 흐름과 무기물의 순환 4.인의 순환 (The Energy Flow and Mineral Cycles In a Zoysia japonica and a Miscanthus sinensis Ecosystem on Mt. Kwanak 4. The Cycles of Phosphorus)

  • 장남기;김정석;강경미
    • 아시안잔디학회지
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    • 제9권4호
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    • pp.275-284
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    • 1995
  • In this investigation, the accumulation mineralization and annual cycle of organic P has been studied in grassland ecosysterns of a Z japonica grassland and a M sinensis grassland on Mt. Kwanak. The basic models of the accumulation and mineralization for ash components of a grass-litter have been presented as the equations (1), (2), and (3). The equations (7)~(10) for organic P are derived from these basic concepts. There was a highty significant relationship between organic matter and organic P. The estimates between organic matter and organic P correlated very high significance. The parameter factors k or k' of mineralization of organic P for the Z. japonica and M sinensis asslands were k=0.412 or k'=0.292 and k=0.224 or k'=0.183, respectively. The time required for a cycle to be completed from organic P to inorganic P of 50, 95 and 99 % are 3.9, 16.7 and 27.8 years in the Z. japonica grassland and 4.1, 17.7 and 29.4 years in the M sinensis grassland. The annual P cycle formulae for mineralization were based on the equations (5), (11) and (12). Annual yields of mineralization for organic P in the steady state grasslands of Z. japonica and M sinensis were 0.407 and 0.504g /$m^2$, respectively.

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해저준설토 사면에서 개량제 처리에 의한 한국들잔디 직파 지표고정 공법에 관한 연구 (Soil Surface Fixation by Direct Sowing of Zoysia japonica with Soil Improvement on the Dredged Soil Slope)

  • 정용호;이임균;서경원;임주훈;김정호;신문현
    • 한국환경복원기술학회지
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    • 제14권4호
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    • pp.1-10
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    • 2011
  • 본 연구는 새만금방조제의 해저준설토 매립지반에서 한국들잔디(Zoysia japonica)에 의한 효율적인 지표고정 공법을 개발하기 위해 수행하였다. 관행적 방법인 줄떼붙이기 시공지(A처리구 : 해저준설토 위에 15cm의 일반 흙을 복토)를 대조구로 하고, 해저준설토와 토양개량제를 혼합한 처리구와 여기에 일반 흙을 추가로 혼합한 처리구 등 생육기반이 다른 3개의 처리구에 한국들잔디를 기계파종 하였다. 관행방식으로 처리한 대조구인 A는 일반 흙을 15cm나 복토한 상태에서 시공한 것임에도 피복률이 43%이었으나 해저준설토 개량처리구인 B(천층)와 C, D(심층) 처리구는 모두 100%로 피복률이 높게 나타났다. 생육상황 분석은 엽장, 엽폭, 뿌리길이에 대해 실시하였는데 토양개량 효과가 뚜렷하였다. 엽장은 대조구 A는 5.73cm, 천층처리구 B는 5.97cm로 유사하였으며, 심층처리구인 C와 D처리구는 27.42cm, 30.18cm로 높게 나타났다. 엽폭은 C와 D처리구가 각각 3.79mm, 3.49mm이었으며 천층처리구 B는 2.40mm, 대조구 A는 1.97mm에 그쳤다. 뿌리길이는 D, C, B처리구에서 각각 13.18cm, 12.20cm, 8.56cm이었으며 대조구 A는 9.38cm이었다. 식물체 내 양분 분석 결과 전질소를 비롯하여 다른 양분함량에 대해서도 토양개량 효과가 확인되었다. 전질소 함량은 심층개량구인 C처리구의 지상부가 1.014%로 가장 높았으며 D와는 근소한 차이를 보였고 천층개량구 B, 대조구 A처리구 순으로 나타났다. 질소는 식물의 생장에 가장 큰 영향을 하는 양분으로서 지상부 생육상황 조사결과와 일치하였으며 지하부는 D처리구가 0.892%, C > A > B처리구 순이었다. 뿌리생장에 중요한 기능을 하는 인산의 함량에 있어서는, C처리구의 지상부가 0.442%로 가장 높았으며 D > B > A 처리구 순으로 나타났고, 지하부는 D > C > A > B처리구 순으로 높게 나타나 전질소와 같은 경향을 보였다. 칼륨 함량은 지상부에서 C > D > B > A 순으로, 지하부에서는 D > C > B > A 순으로 나타나 전질소만큼의 차이는 아니지만 뚜렷한 토양개량 효과를 보였다. 나트륨은 칼슘과 더불어 간척지, 건조지 등에서 토양의 염분집적으로 식물생장에 장애를 일으키는 대표적인 염류로서 토양을 개량하지 않은 대조구 A에서 지상부의 함량이 월등히 높았다. 이에 반해 지하부의 함량은 나트륨, 칼슘 모두 모든 처리구에서 큰 차이를 보이지 않았다. 또한 엽록소의 생성에 영향하는 마그네슘도 모든 처리구에서 유사한 수치를 나타냈는데 지상부는 0.226~0.237%, 지하부는 0.167~0.211%이었다. 이상과 같이 본 연구를 수행함으로써 해저준설토만의 생육기반에 일반 흙을 사용하지 않거나 최소화를 추구하면서 식재가 아닌 파종에 의한 지표고정공법을 개발하였다. 즉, 피복률은 물론 엽장, 엽폭, 뿌리길이 등 생장에서 심층개량구(C와 D)는 물론 천층개량구(B)도 관행방법이나 다른 집약관리 시험지에 비해 아주 우수한 것으로 나타났다. 단 C와 D 심층처리구는 수목식재기반까지 고려한 것이므로 잔디가 필요이상으로 생장한 것이다. 따라서 교목성 수목 식재를 고려하지 않고 지표고정만을 목적으로 한다면 B 처리구가 적합한 것으로 판단된다. 현재 방조제 사면에 대한 지표고정은 해저준설토 위에 산지채취토를 15~30cm 복토하고 한국들잔디(Zoysia japonica)를 평떼나 줄떼붙이기로 시공되어 있는데 특히 줄떼붙이기 시공지의 경우 피복속도가 예상보다 더디고 잡초와의 전쟁을 하고 있으며, 더욱이 일부 구간에는 2종의 녹화매트류가 시험적 규모로 시공된 상태이나 1년도 못되어 완전히 고사하는 등 충분한 지표고정 효과를 보지 못하고 있다. 일반 흙을 전혀 사용하지 않거나 적은 양을 사용하면 훼손산지가 줄게 됨으로 자연을 보호하고 보전하는 효과와 가치는 헤아릴 수 없을 정도로 지대하다고 생각한다. 따라서 관행적으로 시공하고 있는 현행의 지표고정공법은 바꾸어야 할 필요가 있으며, 본 연구결과가 널리 활용되기를 기대한다.

제초제저항성 들잔디(Zoysia japonica Steud.) 이벤트 Jeju Green21의 환경위해성평가 (Environmental risk assessment of genetically modified Herbicide-Tolerant zoysiagrass (Event: Jeju Green21))

  • 배태웅;강홍규;송인자;선현진;고석민;송필순;이효연
    • Journal of Plant Biotechnology
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    • 제38권2호
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    • pp.105-116
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    • 2011
  • Transgenic zoysiagrass (Zoysia japonica Steud.) expressing the bar gene inserted in the plant genome has been generated previously through Agrobacterium tumefaciens-mediated transformation. The GM zoysiagrass (event: JG21) permits efficient management of weed control of widely cultivated zoysiagrass fields, reducing the frequency and cost of using various herbicides for weed control. Now we have carried out the environmental risk assessment of JG21 prior to applying to the governmental regulatory agency for the commercial release of the GM turf grass outside of test plots. The morphological phenotypes, molecular analysis, weediness and gene flow from each test plot of JG21 and wild-type zoysiagrasses have been evaluated by selectively analyzing environmental effects. There were no marked differences in morphological phenotypes between JG21 and wild-type grasses. The JG21 retained its stable integration in the host plant in T1 generation, exhibiting a 3:1 segregation ratio according to the Mendelian genetics. We confirmed the copy number (1) of JG21 by using Southern blot analysis, as the transgenic plants were tolerant to ammonium glufosinate throughout the culture period. From cross-fertilization and gene flow studies, we found a 9% cross-pollination rate at the center of JG21 field and 0% at distances over 3 m from the field. The JG21 and wild-type zoysiagrass plants are not considered "weed" because zoysiagrasses generally are not dominant and do not spread into weedy areas easily. We assessed the horizontal gene transfer (HGT) of the transgene DNA to soil microorganisms from JG21 and wild-type plants. The bar gene was not detected from the total genomic DNA extracted from each rhizosphere soil of GM and non-GM Zoysia grass fields. Through the monitoring of JG21 transgene's unintentional release into the environment, we found no evidence for either pollen mediated gene flow of zoysiagrass or seed dispersal from the test field within a 3 km radius of the natural habitat.

Performance of Three Warm Season Turfgrasses under Linear Gradient Irrigation

  • Ow, Lai Fern;Ghosh, Subhadip
    • Weed & Turfgrass Science
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    • 제6권1호
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    • pp.61-66
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    • 2017
  • The appropriate level of irrigation for turfgrasses is vital to the performance of the turfgrass as well as conservation of water. Linear gradient irrigation system (LGIS) facilitates long-term study of turf performance under continuous irrigation gradients at extreme ends of the irrigation scale. The objectives of this study were to: a) determine the minimum irrigation requirements and relative drought resistance in three warm season turfgrasses; and b) evaluate the medium to long-term effects of irrigation levels on turf persistence, weed invasion, and susceptibility to diseases. Results suggest that grasses differed in drought resistance and persistence under variable irrigation regimes. Irrigation (Ep) required for consistent acceptable turf quality for respective grasses was Cynodon dactylon x C. transvaalensis (61%), Zoysia matrella L. Merr (73%), and Stenotaphrum secundatum 'Palmetto' (86%). Brown patch infection was most prevalent in Stenotaphrum secundatum 'Palmetto' at 12 and 125% Ep irrigation. Cynodon dactylon x C. transvaalensis and Zoysia matrella L. Merr were better able to adapt to the various irrigation regimes, and this ability allowed these species to resist drought, and maintain turf coverage which in turn, kept weeds and the occurrence of diseases at bay. Ranking these grasses for their drought tolerance abilities showed that Cynodon dactylon x C. transvaalensis had the most outstanding resistance against drought, followed by Zoysia matrella L. Merr, and lastly, Stenotaphrum secundatum 'Palmetto'. Despite having the highest irrigation requirement, Stenotaphrum secundatum 'Palmetto' was still not able to maintain persistence at high irrigation regimes. Likewise, this grass also lost turf coverage at low irrigation levels.

질소 시비 수준별 금잔디의 생육과 질소 시비량 설정 (Establishment of a Standard Nitrogen Application Rate for Zoysia matrella Using Growth Responses to Various Fertilization Level)

  • 김영선;최문진;윤정호;이긍주
    • 한국환경농학회지
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    • 제41권3호
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    • pp.167-176
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    • 2022
  • BACKGROUND: Nitrogen (N) is an important element for turfgrass (Zoysia matrella) growth; however, standard N application rate for turfgrass is not established yet. This study was conducted to evaluate the effect of N application rates on the growth and quality of turfgrass for establishment of standard N application rate. METHODS AND RESULTS: Treatments were as follows; control (0 N g/m2/month), 1N (1 N g/m2/month), 2N(2 N g/m2/month), 3N (3 N g/m2/month), 4N (4 N g/m2/month), and 5N (5 N g/m2/month). N application improved visual turfgrass quality. Compared with the control, clipping yield of all N treatments increased by 90~194%. The grass shoot weight of 3N, 4N, and 5N treatments increased by 52%, 43%, and 111%, respectively, and the stolon weight of 4N and 5N treatments increased by 412% and 201%, respectively, compared to the control. The N uptake amount and N recovery rate were estimated to be 4.10~6.28 g/m2 and 14~58%, respectively. CONCLUSION(S): These results indicate that considering visual quality, clipping yield, N uptake amount, and N recovery, the application rate of 2~3 N g/m2/month was suggested to be suitable for Z. matrella production.

질소시용, 예초 및 재식밀도가 한국잔디(Zoysia Japonica Steud)의 생육에 미치는 영향 (Studies on the growth of Korea Lawn Grass (Zoysia japonica Steud.)in Reponse to Nitrogen Application, Clipping Treatment and Plant Density)

  • 심재성
    • 자연과학논문집
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    • 제1권
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    • pp.61-113
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    • 1987
  • The increasing emphasis placed on the production of fine turf for lawns, golf courses, parks, and other recreational sites has led to many unsolved problems as to how such turf could be best established and mainteined. For this purpose, a series of experiments were conducted under con ditions of pot and field. The results obtained were as follows EXPERIMENT I. The effect of nitrogen fertilizer and clipping interval on Zoysia japonica. 1. Increasing the rate of nitrogen and frequent clipping increased tiller number of Zoysis japonica and the maximum number of tillers were obtained from 700 kg N application and freqnent clippings (10 days interval ) in October. Treatment of 350kg N with 10 days clipping interval increased tillers much more than those of 700 kgN with 20 and 30 days clipping intervals. 2. The average number of green leaves occurred during the growth period maximized by applying 700 kg N and clipping 10 days interval. 3. Increasing tiller numbers significantly decreased tops DM weight per tiller by clipping plants at interval of 10 and 20 days, irrespective of nitrogen applied, and with nil N, at the interval of 30 days. By applying 700 kg N, however, top DM weight per tiller increased as the number of tillers increased consistently. 4. The highest top DM weight was achieved from late August to early September by applying 350 and 700kgN. 5. During the growth period, differences in unders ( stolon + root ) DM weight occurred bynitrogen application were found between nil N and two applied nitrogen levels, whereas, at the same level of nitrogen applied, the increase in stolon DM weight enhanced by lengthening the clipping interval to 30 days. 6. Nitrogen efficiency to green leaves, stolon nodes and DM weight of root with high nitrogen was achieved as clipping interval was shortened. 7. By increasing fertilizer nitrogen rate applied, N content n the leaves and stems of Zoysiajaponica was increased. On the other hand, N content in root and stolon had little effect onfertilizer nitrogen, resulting in the lowest content among plant fractions. The largest content of N was recorded in leaves. Lengthening the clipping interval from 10 or 20 to 30 days tends to decrease the N content in the leaves and stems, whereas this trend did not appeared in stolon androot. 8. A positive correlations between N and K contents in tops and stolon were established andthus K content increased as N content in tops and stolon increased. Meanwhile, P content was not affected by N and clipping treatments. 9. Total soluble carbohydrate content in Zoysia japonica was largest in stolon and stem, and was reduced by increasing fertilizer nitrogen rate. Reduction in total soluble carbohydrate due to increased nitrogen rate was severer in the stolons and stems than in the leaves. 10. Increasing the rate of nitrogen applied increased the number of small and large vascular bundles in leaf blade, but shortened distance among the large vascular bundles. Shortening the clipping interval resulted in increase of the number of large vascular bundles but decrease ofdistance between large vascular bundles.EXPERIMENT II. Growth response of Zoysia japonica imposed by different plant densities. 1. Tiller numbers per unit area increased as plant density heightened. Differences in num ber between densities at higher densities than 120 D were of no significance. 2. Tiller numbers per clone attained by 110 days after transplanting were 126 at 40D,77 at 80D, 67 at 120D, 54 at 160D, and 41 at 200D. A decreasing trend of tiller numbers per clone with increasing density was noticable from 100 days after transplanting onwards. 3. During the growth period, the greatest number of green leaves per unit area were attainedin 90days after transplanting at 160D and 200D, and 100 days after transplanting at 40D, 80Dand 120D. Thus the period to reach the maximum green leaf number with the high plantdensity was likely to be earlier that with the low plant density. 4. Stolon growth up to 80 days after transplaning was relatively slow, but from 80 daysonwards, the growth quickened to range from 1.9 m/clone at 40D to 0.6m/clone at 200Din 200 days after transplanting, these followed by the stolon node produced. 5. Plant density did not affect stolon weight/clone and root weight/clone until 80 daysafter transplanting. 6. DM weight of root was heavier in the early period of growth than that of stolon, butthis trend was reversed in the late period of growth : DM weight of stolon was much higherthan that of root.EXPERIMENT Ill. Vegetative growth of Zoysia japonica and Zoysia matrella as affected by nitrogen and clipping height. 1. When no nitrogen was applied to Zoysia japonica, leaf blade which appeared during theAugust-early September period remained green for a perid of about 10 weeks and even leavesemerged in rate September lived for 42 days. However, leaf longevity did not exceed 8 weeks asnitrogen was applied. In contrast the leaf longevity of Zoysia matrella which emerged during the mid August-earlySeptember period was 11 weeks and, under the nitrogen applied, 9 weeks, indicating that thelife-spen of individual leaf of Zoysia matrella may be longer than that of Zoysia japorica. Clipping height had no effect on the leaf longevity in both grasses. 2. During the July-August period, tiller number, green leaf number and DM weightof Zoysia japonica were increased significantly with fertilizer nitrogen, but were not with twolevel of clipping height. This trend was reversed after late September ; no effect of nitrogen wasappeared. Instead, lax clipping increased tiller number, green leaf number and DM weight. Greenleaves stimulated by lax clipping resulted in the occurrance of more dead leaves in late October. 3. Among the stolons outgrown until early September, the primary stolon was not influencedby nitrogen and clipping treatments to produce only 2-3 stolons. However, 1st branch stoIon asaffected by nitrogen increased significantly, so most of stolons which occurred consisted of 1st branch stolons. 4. Until early September, stolon length obtained at nil nitrogen level was chiefly caused bythe primary stolons. By applying nitrogen, the primary stolons of Zoysia japonica waslonger than 1st branch stolons when severe clipping was involved and in turn, shorter than 1stbranch stolons when lax clipping was concerned. In Zoysia matrella, 1st branch stolons were muchlonger than the primary stolon when turf was clipped severely but in conditions of lax clippingthere was little difference in length between primary and 1st branch stolons. 5. Stolon nodes of both Zoysia japonica and Z. matrella were positively influenced by nit rogen, but no particular increase by imposing clipping height treatment was marked in Zoysiamatrella. Although the stolon of Zoysia japonica grew until late October, the growthstimulated by nitrogen was not so remarkable as to exceed that by nil N.

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추석 전 잔디 깍기(벌초) 시기 및 높이에 따른 들잔디와 금잔디의 생육 (Effects of Mowing (Beolcho) Timing and Height on Growth Characteristics of Zoysia japonica and Z. matrella before Chuseok)

  • 장석원;구준학;성창현;이정호;박소준;지재욱;윤정호
    • Weed & Turfgrass Science
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    • 제7권2호
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    • pp.140-147
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    • 2018
  • 우리나라에서 벌초는 추석 성묘와 시향에 앞서 이루어지는 매우 중요한 행사이다. 벌초는 보통 추석 1-2주 전에 집중해서 이루어지기 때문에 전국적으로 도로의 교통체증이 심해지고 안전사고의 발생도 점점 많아지고 있다. 잘못된 벌초 방법은 흙이 드러나 토양 유실을 유발하거나 묘지 잔디의 밀도 저하와 잡초 발생의 원인이 되기도 한다. 다라서 벌초시기의 분산과 올바른 벌초 방법은 매우 중요한 문제일 수 있다. 본 연구에서 벌초 시기는 추석 1주전, 2주전, 3주전, 4주전 잔디 깍기 처리를 두었고, 벌초 방법에서는 잔디 깍는 높이를 달리하여 들잔디와 금잔디의 생육에 미치는 영향을 평가하였다. 들잔디에서 추석과 시향 시기를 기준으로 잔디 깍기 시기는 빠를수록 초장이 길었으나 피복률과 밀도는 높은 경향이었다. 금잔디에서 잔디 깍기 시기는 빠를수록 초장이 길었으나, 피복률과 밀도는 각각 차이가 없거나 일정한 경향을 보이지 않았다. 들잔디에 비해서 금잔디의 초장은 잔디 깍기 시기에 관계없이 짧게 유지되었으며 피복률과 밀도도 높게 유지되었다. 벌초는 들잔디와 금잔디 모두 짧게 할 수록 초장이 짧아졌고, 피복률과 밀도도 낮아졌다. 또한 벌초 시 들잔디와 금잔디는 각각 지상부의 40-50 mm or 30-40 mm 남겨둘수록 묘지 잔디의 밀도유지에 유리한 것으로 나타났다.

잔디 토양전염성병원진균에 대한 길항미생물의 분리 및 길항효과 (Isolation of Antibiotic-producing Microorganisms Antagonistic to Soilborne Pathogenic Fungi of Bentgrass and Their Antifungal Activity)

  • 이용세;전하준;이창호;송치현
    • 한국유기농업학회지
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    • 제6권1호
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    • pp.133-149
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    • 1997
  • Recently, the importance of management and cultivation of grasses has been increased in Korea. Among these cultural practices, the appropriate control of diseases is considered more important than other cultivation techniques such as fertilization and irrigation. The damages of brown patch and large patch caused by Rhizoctonia spp. and Pythium blight caused by Pythium spp. are serious in the major cultivation area of turfgrass in Korea. Since these diseases are difficult to control by agrochemicals, the damages are very serious if these are occured. The periodic spray of agrochemicals, to protect and control these diseases could make some problems of toxicity and environmental pollution as well as rising of non-target diseases. Therefore, the biological methods to control diseases have been required to decrease problems resulted from overuse of agrochemicals, to conserve natural ecosystem, and to control effectively diseases of grasses in the long period. The number of studies about biological control using antagonistic microorganisms have been increased for last half century. However, the application of biological control method has been very limited. In this study, thirteen isolates of R. cerealis, 8 isolates of R. solani and 3 isolates of Phthyn spp. have been isolated from diseased turfgrass in golf course and grass-culture area that have patch and wilting symptoms of zoysia grass and creeping bentgrass. Isolation frequency of R. cerealis and R. solani was high in especially zoysiagrass, while Pythym spp. was isolated from bent grass at low frequency but showed high pathogenicity. Totally, 205 isolates of soil microorganisms were isolated in this study as primary antagonistic microorganism by Herr's triple agar layer plate and dual culture method using rhizosphere of grasses, soil of crop field as the source of antagonistic microorganisms. Among the 205 isolates, 23 isolates were actinomycetes and 182 isolates were bacteria. All of the actinomycetes were isolated by Herr's method. Antagonistic effect of primary isolated microorganisms was tested for in vitro mycelial growth inhibition against pathogenic fungi isolated from grasses and for inhibition of disease occurrence in 24 well tissue culture plate and pot experiment. Then, four isolated of bacteria which are BG23, BG74, BG136 and BG171 were selected as antagonistic microorganisms against soil-born pathogenic fungi of bentgrass.

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