• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2005년도 춘계 학술발표회 논문집
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• pp.107-112
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• 2005

Road is built continuously along with development of industry and cut slope is happened necessarily in road construction. Geoengineers are executing cut slope stability analysis considering various cut slope condition such as topography, geology, hydraulic condition and so on. The Tertiary Jungja Basin is located in the southeastern coastal area of the Korea Peninsula. Jungja Basin area is created by geotectonic movement of the plate after Early Miocene epoch. The northwestern and southwestern boundary of the basin is fault zone. The Basement rock is hornfels (Ulsan Formation). Basin-fills consist of extrusive volcanic rock(Tangsa Andesites), unconsolidated fluviatile conglomerate(Kangdong Formation) and shallow brackish-water sandstone(Sinhyun Formation). The characteristics of cut slopes in this area is different with cut slopes in the other site. Soil layers in this area is unconsolidated sediments and is not formed the weathering and erosion of the rock. So, the depth of soil layer is very thick. Faults of this area are northwest-southeast and northeast-southwest direction. Expandible clay mineral as smectite, chlorite et al. detected from fault gouge using XRD. Therefore, Jungja Basin area must consider the characteristics of the faults and soil layers thickness necessarily cut slopes stability analysis.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.576-581
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• 2005

Phytase improves the bioavailability of phytate phosphorus in plant foods to humans and animals, and reduces the phosphorus pollution of animal waste. We have engineered the cell surface of the yeast. Saccharomyces cerevisiae, by anchoring active fungal phytase on its cell wall, in order to apply it as a dietary supplement containing bioconversional functions in animal foods and a whole cell bio-catalyst for the treatment of waste. The phytase gene (phyA) of Aspergillus niger with a signal peptide of rice amylase 1A (Ramy1A) was fused with the gene encoding the C-terminal half (320 amino acid residues from the C-terminus) of yeast ${\alpha}-agglutinin$, a protein which is involved in mating and is covalently anchored to the cell wall. The resulting fusion construct was introduced into S. cerevisiae and expressed under the control of the constitutive glyceraldehydes-3-phosphate dehydrogenase (GPD) promoter. Phytase plate assay revealed that the surface-engineered cell exhibited a catalytically active opaque zone which was restricted to the margin of the colony. Additionally, the phytase activity was detected in the cell fraction, but was not detected in the culture medium when it was grown in liquid. These results indicate that the phytase was successfully anchored to the cell surface of yeast and was displayed as its active form. The amount of recombinant phytase on the surface of yeast cells was estimated to be 16,000 molecules per cell.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.462-467
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• 2007

From the research which it sees verification of the whole interpretation and local interpretation of the durability steel deck bridge a static test and it produces the test body which it sells with character and it executes smallness pul lek detailed interpretation it leads and the appropriate characteristic of smallness pul lek detailed interpretation and to sleep a nominal stress price and it compares it judges it does. The stress quality from each structure region which it follows in load stock location it analyzes and from the hazard which evaluates, the objective region the length rib and the bottom grater weld zone, the length rib and width rib connection department and the width rib with the father it divided. It investigated the stress distribution of the test body from these objective location, FEM interpretation it led and the conduct against each structure state tax it analyzed. General conduct the load stock location the floor plate is located in the center with interpretation price together symmetry characteristic to seem, it cannot be like that it cannot there is one actual test price. Like this reason the length rib and width rib connection actually production even production characteristic security it is a day when it is impossible with the curvature junction department which it blows, it follows in examination body deferment condition and form feed with the fact that it is visible a big difference even with error of some it becomes. Consequently for a data and the research which are more accurate it is judged with the fact that the effort which is prudent will be necessary.

• 한국지구물리탐사학회:학술대회논문집
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• 한국지구물리탐사학회 2006년도 공동학술대회 논문집
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• pp.95-100
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• 2006

울산단층의 지하구조를 확인하기 위하여 울산시 농소읍 부근에서 750 m의 측선을 따라 탄성파 자료를 획득하였다. 5 kg의 해머를 지표에 비스듬히 설치된 알루미늄 플레이트에 타격하여 P파와 S파를 동시에 발생시켰으며, 3 m 간격의 10 Hz 3성분 지오폰 16개로 수신하였다. P파 굴절파 자료는 토모그래피 방식으로 역산하였으며, 반사파 자료는 통상적인 처리과정에 경사경로시차보정, 구조보정 등의 과정을 추가하였다. P파 굴절파로부터 구한 속도정보와 S파 및 P파 반사파 중합단면을 해석하여 4개 층을 구분하였다. 상부로부터 각 층의 P파 속도는 $300{\sim}1100\;m/s$, $1100{\sim}1700\;m/s$, $1700{\sim}2700\;m/s$, 2700 m/s 이상이고, 상부 3개 층의 두께는 각각 평균 3.9 m, 5.9 m, 4.4 m 정도이다. S파 중합단면은 10 m 이내의 지질구조 규명에 효과적이었다.

• 한국축산식품학회지
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• 제30권5호
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• pp.804-810
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• 2010

This study was performed to select protease-producing Bacillus sp. as a potential probiotic starter for fermented meat products. In order to isolate protease-producing bacterium from meju, measured the diameter of the clear zone on agar plate (TSA, 1% (w/v) skim milk) and analyzed for intracellular protease activity, then 10 Bacillus-like strains were isolated. Three Bacillus-like strains (P19, P27, and P33) among 10 strains were able to tolerate in acidic condition (TSB, pH 2.5, 2 h incubation). These 3 strains were showed antimicrobial activity against food-borne pathogenic bacteria. These vegetative cells of 3 strains were showed a survival rate of 0.04% to 0.08% under the artificial gastric acidic condition (TSB, pH 2.5 with 1% (w/v) pepsin), but spore-forming cells were 56.29% to 84.77%. Vegetative cells of 3 strains were the least bile-resistant, while spore-forming cells of 3 strains showed higher survival rate more than 76% under artificial bile condition (TSB, 0.1% (w/v) oxgall bile). In these strains, P27 strain was finally selected as a good probiotic strain. P27 strain was tentatively identified as Bacillus amyloliquefaciens by API CHB kit and 16S rDNA sequence analysis. The results of this study suggest that B. amyloliquefaciens P27 can be used as a potential probiotic starter for fermented meat product.

• 한국안전학회지
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• 제5권1호
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• pp.19-29
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• 1990

The objective of this paper is to investigate the effect of residual stresses on the $\Delta$K$\sub$th/ and fatigue crack growth behavior of butt weldments. For this purpose, transverse butt sutmerged arc welding was performed on SM50A steel plate and CT(compact tension) specimens which loading direction is perpendicular to weld bead were selected. Welding residual stresses distribution on the specimen was determined by hole drilling method. The case of crack located parallel to weld bead, the states of as weld and PWHT, $\Delta$K$\sub$th/ of specimens(HAZ, weld zone) was higher than that of the base metal probably because of the compressive residual stresses of crack tip. In low $\Delta$K region, it is estimated that the effects of residual stresses for da/dN are great. In region II, the da/dN of weldments in as weld state was lower than that of the base metal. Though da/dN of Weldments in PWHT state was similar to that of the base metal. The constant of power law, m in two states consisted with the base metal. Therefore , it is estimated that the value of m is not affected by residual stresses. Fatigue crack growth behavior of weldments consisted with the base metal considering the effective stress intensity factor range($\Delta$K$\sub$eff/) included the effect of initial residual stress(Kres). Thus, we can predict the fatigue crack growth behavior of weldment by knowing the distribution of initial residual stress at the crack tip.

• 한국터널지하공간학회 논문집
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• 제6권2호
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• pp.171-182
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• 2004

사질토지반에서 2 Arch 터널을 굴착할 때에 중앙터널의 굴착규모와 인버트 폐합에 따른 중앙필러 및 터널 주변지반의 거동특성을 규명하기 위하여 모형실험을 수행하였다. 3개로 분리되어 수직이동이 가능한 가동판 위에 중앙필러와 모형라이닝을 설치하고 모형지반 조성 후에 가동판을 강하시켜서 중앙터널굴착을 표현하였고, 중앙터널굴착 후에 좌 우측 터널을 굴착하였으며 굴착이 완료된 2 Arch 터널 전체에 대한 가동판을 움직여서 2 Arch 터널굴착에 따른 하중전이 및 지반이완 형태는 물론 중앙필러에 작용하는 하중의 변화를 관찰하였다. 실험중에 가동판, 중앙필러 및 바닥판에 작용하는 이완하중 및 전이하중을 측정하였고, 모형터널의 내부와 지중 및 지표에 변위계를 설치하여 모형터널의 내공변위 및 지반의 변위를 측정하였다. 본 연구결과 시공순서와 라이닝의 기초부 구속조건에 따라 중앙필러가 부담하게 되는 하중의 변화를 확인하였고 2 Arch 터널굴착에 따른 하중전이 및 지반이완형태를 확인하였다.

• 한국기계가공학회지
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• 제19권7호
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• pp.89-97
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• 2020

Container vessels continue to grow in size, led by global shipowner. Large ships can be loaded more cargo at a time, reducing the cost of transportation per teu. this eventually leads to economies of sale, in which the production cost per unit decreases with increasing output. in accordance with the 70th Convention of the Marine Environment Protection Committee of the International Maritime Organization, as of January 1, 2020, MARPOL Annex VI Regulation 14.1.3 will be effective. All vessels must be meet these criteria to reduce Sox emissions and reduce NOx emissions by reducing the content of manned sulfur oxides from 3.5% to less than 0.5%, otherwise IACS Member States Entry to the port is denied. in order to do that need to LNG storage tank. in this study characteristic of the material after line heating (600℃,700℃,800℃,900℃) of 9% Ni steel used in the manufacture of LNG fuel tank of ship were verified using by mechanical test. In the heating method by line heating. The initial properties of steel are changed by variables such as temperature, time, speed. The experimental data of line heating presented in this paper confirmed that the initial change of 9% Ni steel could be minimized.

• 한국조경학회지
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• 제30권5호
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• pp.89-97
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• 2002

This report describes the first prize design of Midongsan Eco-park, which is located in Chungcheongbuk-do (North Chungcheong) province. Criteria of the winning design are as follows: 1) The design should include a neighborhood park as well as a portion of a green zone, in order to harmonize the area ecosystem, gradually assimilate the surroundings, and serve as an education tool for the public. 2) According to an analysis of area maps and a site-inspection, a preserved area, a buffer area, a restored area, and a developed area should be developed. In the buffer and restored areas, major facilities and activity spaces should be set up. 3) Observation trails should be accessible to visitors, and a variety of adventure programs should be available. 4) The eco-park should be designed to provide a habitat for the Gorani (Chinese water deer) which used to live around Midongsan. 5) For sensuous and vivid visitor experiences, diverse programs are to be designed to accommodate different seasons, visitor ages, and required time. Environment and eco-education are to be emphasized to maximize the effect of the eco-programs. 6) Optimal activities for visitors of different ages, optimal time intervals and eco-programs by age are also suggested. 7) Facilities are zoned into three sections according to level of development. Each section has a main area and supplementary areas for better understanding. 8) Nature-friendly arrangement of facilities and construction should minimize earthwork and adverse effects on the local ecology. Throughout the planning, the following things were learned: longer-term analysis including all season's observation is essential; planning, designing, bui Iding and managing should be based on this careful, extended observation. The contest should have taken plate over a longer period of time in order enable more detailed planing of the eco-park For improved management and activities, a volunteer system in linked with schools eco-institutions and NGOs should be developed with eco-interpreters.

• Journal of Microbiology and Biotechnology
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• 제17권9호
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• pp.1469-1476
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• 2007

Jeot-gal is a traditional Korean fermented seafood and has long been used for seasoning. We isolated 188 strains from shrimp, anchovy, and yellow corvina Jeot-gal, and screened sixteen strains that showed strong fibrinolytic activities on a fibrin plate. Among those strains, the strain that had the largest halo zone was chosen and identified as Bacillus licheniformis by using 16S rDNA sequencing and an API CHB kit. The fibrinolytic activity of Bacillus licheniformis was characterized and designated as bpKJ-31. The active component of bpKJ-31 was identified as a 37 kDa protein, designated bacillopeptidase F, by internal peptide mapping and N-terminal sequencing. The optimum activity of bpKJ-31 was shown at pH 9 and $40^{\circ}C$, with a chromogenic substrate for plasmin. It had high degrading activity for the $B{\beta}$-chain and $A{\alpha}$-chain of fibrin(ogen), and also acted on thrombin, but not skim milk and casein. The amidolytic activity of bpKJ-31 was inhibited by 1 mM phenylmethanesulfonyl fluoride, but 1 mM EDTA did not affect the enzyme activity, indicating that bpKJ-31 is an alkaline serine protease, like a plasmin. The bpKJ-31 showed approximately 14.3% higher fibrinolytic activity than the plasmin. These features of bpKJ-31 make it attractive as a health-promoting biomaterial.