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Cadmium Adsorption by Natural Zeolite (천연(天然) Zeolite에 의(依)한 Cadmium의 흡착(吸着))

  • Kim, Young-Kyung;Lee, Jyung-Jae;Choi, Jyung
    • Korean Journal of Environmental Agriculture
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    • v.5 no.2
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    • pp.101-105
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    • 1986
  • An adsorption and desorption experiment was conducted to utilize natural zeolite as cadmium adsorbent in wastewater. Adsorption of cadmium by natural zeolite was conformed to Freundlich's adsorption equation and natural zeolite was found to be effective adsorbent. The higher the cadmium concentration of solution, the more the adsorption amount of cadmium was and the adsorption was in the order of $Ca-<Nontreated-<H-{\leqq}Al-<Nazeolite$. Ion selectivity of natural zeolite in mixed solution increased in the order of Cd$NaCl<CaCl_2<AlCl_3<HCl$.

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Chemical Characteristics and Enzyme Activities of Icheon Ge-Geol Radish, Gangwha Turnip, and Korean Radish (이천 게걸무, 강화 순무, 조선무의 화학적 특성 및 효소활성)

  • Kim, Haeng-Ran;Lee, Ji-Hyun;Kim, Yang-Suk;Kim, Kyung-Mi
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.255-259
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    • 2007
  • For this investigation, we analyzed the chemical characteristics and enzyme activities of Icheon Ge-Geol radish, Gangwha turnip, and Korean radish. Regarding their proximate compositions, the water contents of the Icheon Ge-Geol radish, Gangwha turnip, and Korean radish were 87.78, 92.73, and 91.45%, respectively. The crude protein, crude fiber, and ash contents of the Icheon Ge-Geol radish were 1.35, 1.11, and 1.55%, respectively, which were much higher than the contents of the other samples; however, total dietary fiber was significantly lower. For mineral content, the magnesium, potassium, and calcium levels of the Icheon Ge-Geol radish were higher than the other samples. With regards to the enzyme activities of the samples, the protease and myrosinase activities of the Icheon Ge-Geol radish were higher than in the other samples. For ${\alpha}-amylase$ activity, the Korean radish showed the strongest activity (18.99 units/g of sample), followed by the Gangwha turnip and Icheon Ge-Geol radish.

Studies for Component Analysis, Antioxidative Activity and ${\alpha}-glucosidase$ Inhibitory Activity from Equisetum arvense (쇠뜨기(Equisetum arvense) 추출물의 항산화 활성 분석 및 ${\alpha}-glucosidase$ 저해활성)

  • Gua, Jia;Jin, Ying-Shan;Han, Woong;Shim, Tae-Heum;Sa, Jae-Hoon;Wang, Myeong-Hyeon
    • Applied Biological Chemistry
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    • v.49 no.1
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    • pp.77-81
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    • 2006
  • This study was carried out to investigate the chemical components, and antioxidative and $anti-{\alpha}-glucosidase$ activities of Equisetum arvense extracts. In Equisetum arvense extracts were composed of 53.20% of crude fiber, 20.42% of crude ash, 15.32% of crude protein and 2.21% of crude fat. Potassium was the most predominant mineral and followed by phosphorus, calcium, magnesium, and sodium. The contents of the unsaturated fatty acids, such as linolenic acid, linoleic acid, and palmitic acid, were higher than those of saturated fatty acids. Seventy percent ethanol extract exhibited antioxidative activity with $IC_{50}$ of $168.1\;{\mu}g/ml$. The Seventy percent methanol extract showed higher ${\alpha}-glucosidase$ inhibitory activity than other solvent extracts.

Release of Heavy Metals into Water from the Resuspension of Coastal Sediment (연안 오염퇴적물의 재부상에 의한 중금속의 수계용출특성)

  • Song, Young-Chae;Subha, Bakthavachallam;Woo, Jung-Hui
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.7
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    • pp.469-475
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    • 2014
  • The study investigated the physicochemical characteristics and the ecological risk of the Northport sediment in B city and the releasing properties of heavy metals into seawater during the resuspension also studied. The major components of the sediment are fine silt and clay which contains high organic matter and AVS (Acid volatile sulfide) and the ecological risk of the heavy metals in sediment also very high. The release rate of heavy metals into seawater was in order of Pb>>Cu>Cr>>Zn>Cd during the resuspension in a batch experiment, and the heavy metal release mainly attributed to the oxidation of metal sulfides. Heavy metals which came from easily oxidisable metal sulfides rapidly contaminated seawater within about 1.0 h of the sediment resuspension. The sulfide oxidation during the resuspension increased the residual fraction of heavy metals in the sediment, decreased the organic bound fraction, and changed the other fractions of heavy metals in the sediment. The release of heavy metals from the sediment during resuspension was affected by the resuspension time, the oxidation rate of metal sulfides and resuspended concentration of the sediment particle.

Identification of PM10 Chemical Characteristics and Sources and Estimation of their Contributions in a Seoul Metropolitan Subway Station (서울시 지하역사에서 PM10의 화학적 특성과 오염원의 확인 및 기여도 추정)

  • Park, Seul-Ba-Sen-Na;Lee, Tae-Jung;Ko, Hyun-Ki;Bae, Sung-Joon;Kim, Shin-Do;Park, Duckshin;Sohn, Jong-Ryeul;Kim, Dong-Sool
    • Journal of Korean Society for Atmospheric Environment
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    • v.29 no.1
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    • pp.74-85
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    • 2013
  • Since the underground transportation system is a closed environment, indoor air quality problems may seriously affect many passengers' health. The purpose of this study was to understand $PM_{10}$ characteristics in the underground air environment and further to quantitatively estimate $PM_{10}$ source contributions in a Seoul Metropolitan subway station. The $PM_{10}$ was intensively collected on various filters with $PM_{10}$ aerosol samplers to obtain sufficient samples for its chemical analysis. Sampling was carried out in the M station on the Line-4 from April 21 to 28, July 13 to 21, and October 11 to 19 in the year of 2010 and January 11 to 17 in the year of 2011. The aerosol filter samples were then analyzed for metals, water soluble ions, and carbon components. The 29 chemical species (OC1, OC2, OC3, OC4, CC, PC, EC, Ag, Al, Ba, Cd, Cr, Cu, Fe, Mn, Ni, Pb, Si, Ti, V, Zn, $Cl^-$, $NO_3{^-}$, $SO_4{^{2-}}$, $Na^+$, $NH_4{^+}$, $K^+$, $Mg^{2+}$, $Ca^{2+}$) were analyzed by using ICP-AES, IC, and TOR after proper pretreatments of each sample filter. Based on the chemical information, positive matrix factorization (PMF) model was applied to identify the $PM_{10}$ sources and then six sources such as biomass burning, outdoor, vehicle, soil and road dust, secondary aerosol, ferrous, and brakewear related source were classified. The contributions rate of their sources in tunnel are 4.0%, 5.8%, 1.6%, 17.9%, 13.8% and 56.9% in order.

Molecular and Biochemical Characteristics of ${\beta}$-Propeller Phytase from Marine Pseudomonas sp. BS10-3 and Its Potential Application for Animal Feed Additives

  • Nam, Seung-Jeung;Kim, Young-Ok;Ko, Tea-Kyung;Kang, Jin-Ku;Chun, Kwang-Hoon;Auh, Joong-Hyuck;Lee, Chul-Soon;Lee, In-Kyu;Park, Sunghoon;Oh, Byung-Chul
    • Journal of Microbiology and Biotechnology
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    • v.24 no.10
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    • pp.1413-1420
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    • 2014
  • Phytate is an antinutritional factor that impacts the bioavailability of essential minerals such as $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ by forming insoluble mineral-phytate salts. These insoluble mineral-phytate salts are hydrolyzed rarely by monogastric animals, because they lack the hydrolyzing phytases and thus excrete the majority of them. The ${\beta}$-propeller phytases (BPPs) hydrolyze these insoluble mineral-phytate salts efficiently. In this study, we cloned a novel BPP gene from a marine Pseudomonas sp. This Pseudomonas BPP gene (PsBPP) had low sequence identity with other known phytases and contained an extra internal repeat domain (residues 24-279) and a typical BPP domain (residues 280-634) at the C-terminus. Structure-based sequence alignment suggested that the N-terminal repeat domain did not possess the active-site residues, whereas the C-terminal BPP domain contained multiple calcium-binding sites, which provide a favorable electrostatic environment for substrate binding and catalytic activity. Thus, we overexpressed the BPP domain from Pseudomonas sp. to potentially hydrolyze insoluble mineral-phytate salts. Purified recombinant PsBPP required $Ca^{2+}$ or $Fe^{2+}$ for phytase activity, indicating that PsBPP hydrolyzes insoluble $Fe^{2+}$-phytate or $Ca^{2+}$-phytate salts. The optimal temperature and pH for the hydrolysis of $Ca^{2+}$-phytate by PsBPP were $50^{\circ}C$ and 6.0, respectively. Biochemical and kinetic studies clearly showed that PsBPP efficiently hydrolyzed $Ca^{2+}$-phytate salts and yielded myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products. Finally, we showed that PsBPP was highly effective for hydrolyzing rice bran with high phytate content. Taken together, our results suggest that PsBPP has great potential in the animal feed industry for reducing phytates.

Integrated Eco-Engineering Design for Sustainable Management of Fecal Sludge and Domestic Wastewater

  • Koottatep, Thammarat;Polprasert, Chongrak;Laugesen, Carsten H.
    • Journal of Wetlands Research
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    • v.9 no.1
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    • pp.69-78
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    • 2007
  • Constructed wetlands and other aquatic systems have been successfully used for waste and wastewater treatment in either temperate or tropical regions. To treat waste or wastewater in a sustainable manner, the integrated eco-engineering designs are explained in this paper with 2 case studies: (i) a combination of vertical-flow constructed wetland (CW) with plant irrigation systemfor fecal sludge management and (ii) integrated CW units with landscaping at full-scale application for domestic wastewater treatment. The pilot-scale study of fecal sludge management employed 3 vertical-flow CW units, each with a dimension of $5{\times}5{\times}0.65m$ (width ${\times}$ length ${\times}$ media depth) and planted with cattails (Typha augustifolia). At the solid loading rate of 250 kg total solids (TS)/$m^2.yr$ and a 6-day percolate impoundment, the CW system could achieve chemical oxygen demand (COD), TS and total Kjeldahl nitrogen (TKN) removal efficiencies in the range of 80 - 96%. The accumulated sludge layers of about 80 - 90 cm was found at the CW bed surface after operating the CW units for 7 years, but no clogging problem has been observed. The CW percolate was applied to 16 irrigation Sunflower plant (Helianthus annuus) plots, each with a dimension of $4.5{\times}4.5m$ ($width{\times}length$). In the study, the CW percolate were fed to the treatment plots at the application rate of 7.5 mm/day but the percolate was mixed with tap water at different ratio of 20%, 80% and 100%. Based on a 1-year data of 3-crop plantation were experimented, the contents of Zn, Mn and Cu in soil of the experimental plots were found to increase with increasing in CW percolate ratios. The highest plant biomass yield and oil content of 1,000 kg/ha and 35%, respectively, were obtained from the plots fed with 20% or 50% of the CW percolate, whereas no accumulation of heavy metals in the plant tissues (i.e. leaves, stems and flowers) of the sunflower is found. In addition to the pilot-scale and field experiments, a case study of the integrated CW systems for wastewater treatment at Phi Phi Island (a Tsunami-hit area), Krabi province, Thailand is illustrated. The $5,200-m^2$ CW systems on Phi Phi Island are not only for treatment of $400m^3/day$ wastewater from hotels, households or other domestic activities, but also incorporating public consultation in the design processes, resulting in introducing the aesthetic landscaping as well as reusing of the treated effluent for irrigating green areas on the Island.

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최소가공 과일의 갈변저해를 위한 저해제의 탐색

  • 박연주;최소영;김영언;문광덕
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.119.1-119
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    • 2003
  • 단체 급식의 증가와 더불어 편의식품을 선호하는 소비 경향에 맞추어 최소가공한 과일의 갈변저해를 위한 저해제를 탐색하였다. 실험에 사용한 과일은 배와 감으로 각각 박피하여 속심을 제거한 후 배는 8조각으로 절단하고 감은 1~2cm 두께로 slice 하였다. 저해제는 1% ascorbic acid, citric acid, NaCl, MgCl$_2$, CaCl$_2$, oxalic acid, EDTA, raffinose, salicylic acit, sporix를 사용하였다. 또한 열수 추출한 오약, 계피, 황기, 당기, 건칡, 진피, 천궁, 감초 등도 저해제로 사용하였다. 최소가공한 과일은 저해제에 3분간 침지한 후 물기를 제거하고 tray에 포장하여 실온에서 저장하면서 colorimeter를 이용하여 갈변도의 변화를 측정하였다. 갈변 정도를 나타내는 $\Delta$E 는 시간의 경과에 따라 점차 감소하는 경향을 보였으나 배의 경우 변화 정도가 크지 않았다. NaCl, MgCl$_2$, CaCl$_2$를 처리한 배의 갈변 정도가 가장 적었다. cysteine을 처리한 구에서는 황화합물 냄새가 났다. 또한 oxalic acid를 처리한 구에서는 연화가 빠르게 일어났으며 저장 시간이 경과함에 따라 수분이 용출되었다. 한약재는 자체 색으로 인해 배의 색변화를 증가시켰으며 이취가 발생했다. 또한 저장 시간이 경과함에 따라 건조가 빠르게 일어나 관능적으로도 좋지 않은 결과를 나타냈다. 감에서는 CaCl$_2$, MgCl$_2$를 처리한 구에서 색의 변화가 가장 적었으며 cysteine은 색변화는 적었으나 배의 경우과 같이 이취가 발생하였다. 한약제는 계피와 진피를 처리한 감에서 색의 변화를 저해하는 효과를 보였다.를 처리하였을 경우 당귀, 천문동을 처리한 구에서 효과를 보였다. oxalic acid는 갈변저해에는 효과적이었으나 시간이 경과함에 따라 수분의 용출이 증가하였으며 물성의 변화도 컸다. 또한 ZnCl$_2$를 처리한 구에서는 이취 발생과 수분 용출이 컸으며 색의 변화도 컸다.내었다. 고온 단시간 처리구의 경우 저온 장시간 처리구와는 달리 저장 1일 후부터 그 농도가 다소 상승되었다가 다시 감소는 경향을 보였다. 한편 열처리하여 냉각시킨 직후 및 저장1주 후 사과의 pH, 산도, 당도, 경도, 과육의 갈변도 등을 조사하였던 바 대조구 및 처리구간에 일부 항목에 있어서는 약간의 차이를 나타내었다. 대치할 수 있을 것이라고 사료된다. 수분함량은 기계적 검사보다 관능검사와 더욱 높은 상관관계를 나타냈다.내었다. 항균활성이 우수한 생약재를 농도별로 활성을 조사한 결과, 물 추출물과 10% Ethanol 추출물 모두 낮은 농도에서도 우수한 항균활성을 나타내었다.취와 함께 점질성 갈변물질이 생성되었다. 이와 같은 결과로 볼 때, BAAG의 처리는 BAAC의 경우보다 가격은 저렴하면서도 항균력은 우수한 천연 항균복합제재로써 농산물 식품원료에 적용하여 선도유지 기간을 연장할 수 있는 효과를 기대할 수 있었다. 과일 등의 포장제로서 이용할 가능성을 확인하였다.로 [-wh] 겹의문사는 복수 의미를 지닐 수 없 다. 그러면 단수 의미는 어떻게 생성되는가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([$[W1]_{XO-}$ $[W1]_{XO}$ ]

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A Study on Superoxide Dismutase from various Tissue of the Tricarboxylic acid cycle blocked Rat (Tricarboxylic acid회로를 차단한 흰쥐의 조직에서 Superoxide Dismutase에 관한 연구)

  • Kim, Yil
    • Korean Journal of Microbiology
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    • v.23 no.1
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    • pp.69-76
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    • 1985
  • This study was carried out to observe the formation of superoxide radicals and the changes in the activities of superoxide dismutase (EC.1.15.1.1.) from the various organs of a rat which was blocked tricarboxylic acid cycle. In order to block the tricarboxylic acid cycle, the beta-fluoroethylacetate was injected into peritoneal cavity of rat and removed the various tissues from the rat at internals of an hour. By tissue extracts being prepared by the method of Weigiger and Fridovich the activities of superoxide dismutase, aconitase, and contents of bliid glucose, citrates, and wuperoxide radicals were determined. The experimental results are summarized as follows: Accumulation of citrates if increased within three hours after treatment in the all tested tissues, especially, in the geart and spleen they are higher than one of other tissues as 12 and 20 times of control. The activities of aconitase are ingibited to 30-35% on an hour after beta-fluoroethylacetate treatment comparing with that of control rat. The content of blood glucose is increased to 1.6 fold of normal value after 5 hours of treatment. In all tested tissues, superoxide radicals are formed in the heart as 0.26 micromoles per gram tissue between one and three hours after treatment. The activities of total superoxide dismutase are increased between one and three hours after treatment in the all tested tissues and one of these enzymes in heart is highest. The activities of superoxide dismutase containing Mn are also increased with an increase of total superoxide dismutase activities.

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Change in Geochemical Characteristics of Surface Sediments in the Nakdong River Main Stream (낙동강 본류에 분포하는 표층 퇴적물의 지화학적 특성 변화)

  • Kim, Shin;Lee, Kwonchul;Kim, Jueon;Jung, Kangyoung;Ahn, Jungmin;Kim, Hyounggeun;Lee, Injung;Shin, Dongseok;Yang, Deukseok
    • Journal of Environmental Science International
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    • v.26 no.3
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    • pp.311-324
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    • 2017
  • To certificate change in the geochemical characteristics of surface sediments in the main stream of the Nakdong River, surface sediments from 12 sampling sites during the first and second half year (total 24 sampling sites) were collected and analyzed for grain size, ignition loss, total organic carbon and heavy metal content. Surface sediments mainly composed of sand (coarse and medium sand) and fining changed from the first half to the second half of the year. Ignition loss, total organic carbon and heavy metals content increased in the second half of the year. Some heavy metals (Zn, Ni and Cu) were found to be at the lowest effect levels according to Ontario sediment quality guidelines. Additionally, most heavy metals were found to be at the non polluted level and level I according to USEPA sediment quality standards and National Institute of Environmental Research sediment pollution evaluation standard, respectively. The enrichment factor (< 1) and index of geoaccmulation (< 0) were non polluted in the study area. The correlation analysis results showed that ignition loss, total organic carbon and heavy metal content were highly correlated with grain size. Regarding changes in geochemical characteristics of surface sediments in the study area, grain size fine and organic matter and heavy metal content increased in the second half year. Nonetheless these results indicated pollution levels that did not adversely affect the benthos.