• Nuclear Medicine and Molecular Imaging
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• v.43 no.4
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• pp.337-343
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• 2009

Purpose: ((R)-1-(2-chlorophenyl)-N-1-[$^{11}$C]methyl-N(1-propyl)-3-isoquinoline carboxamide ((R)-PK11195) is a specific ligand for the peripheral type benzodiazepine receptor and a marker of activated microglia, used to measure inflammation in neurologic disorders. We report here that a direct and simple radiosynthesis of [$^{11}$C](R)-PK11195 in mild condition using NaH suspension in DMF and one-step loop method. Materials and Methods: (R)-N-Desmethyl-PK11195 (1 mg) in DMSO (0.1 mL) and NaH suspension in DMF (0.1 mL) were injected into a semi-prep HPLC loop. [$^{11}$C]methyl iodide was passed through HPLC loop at room temperature. Purification was performed using semi-preparative HPLC. Aliquots eluted at 11.3 min were collected and analyzed by analytical HPLC and mass spectrometer. Results: The labeling efficiency of [$^{11}$C](R)-PK11195 was 71.8$\pm$8.5%. The specific activity was 11.8:$\pm$6.4 GBq/$\mu$mol and radiochemical purity was higher than 99.2%. The mass spectrum of the product eluted at 11.3 min showed m/z peaks at 353.1 (M+1), indicating the mass and structure of (R)-PK11195. Conclusion: By the one-step loop method with the [$^{11}$C]CH3l automated synthesis module, [$^{11}C$](R)-PK11195 could be easily prepared in high radiochemical yield using NaH suspension in DMF.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.3
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• pp.244-249
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• 2011

A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by $^1H$, $^{13}C$ NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column ($4.6mm{\times}50mm$, $1.8{\mu}m$) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 ${\rightarrow}$ 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.

• Restorative Dentistry and Endodontics
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• v.36 no.6
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• pp.483-489
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• 2011

Objectives: The purpose of this study was to examine the effect of glycerin topical application on the surface hardness of composite after curing. Materials and Methods: A composite (Z-250, 3M ESPE) was packed into a disc-shaped brass mold and light cured according to one of the following protocols. Group 1 (control) was exposed to air and light cured for 40 sec, group 2 was covered with a Mylar strip and light cured for 40 sec, group 3 was surface coated with glycerin and light cured for 40 sec, and group 4 was exposed to air and light cured for 20 sec and then surface coated with glycerin and cured for additional 20 sec. Twenty specimens were prepared for each group. The surface hardnesses of specimens were measured with or without polishing. Five days later, the surface hardness of each specimen was measured again. Data were analyzed by three-way ANOVA and Tukey's post hoc tests. Results: The surface hardnesses of the unpolished specimens immediately after curing decreased in the following order: group 2 > 3 > 4 > 1. For the polished specimens, there was no significant difference among the groups. Within the same group, the hardness measured after five days was increased compared to that immediately after curing, and the polished specimens showed greater hardness than did the unpolished specimens. Conclusions: The most effective way to increase the surface hardness of composite is polishing after curing. The uses of a Mylar strip or glycerin topical application before curing is recommended.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.33 no.4
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• pp.87-98
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• 2015

The natural monument forests (no.374) located at Pyeongdae-ri in Jeju island are described and classified by using phytosociological methods and numerical analysis. The purpose of this paper is to identify the ecological character of Torreya nucifera forests between natural habitat and artificial habitat, as well as their spatial and phytogeographical distribution in the Korea. The comparison of forests between Pyeongdae-ri and other regions was analyzed by using a non-metric multidimensional scaling analysis (NMDS) and hierarchical clustering. On the basis of the 12 phytosociological $relev{\acute{e}}s$, the vegetation of T. nucifera dominant forest in Jeju island was arranged in one syntaxon (Alangium platanifolium-Torreya nucifera community included typicum and one subcommunity) within Camellietea. The community of T. nucifera dominant forests were characterized floristically and ecologically. We discussed diagnostic species with references, and proposed a few important diagnostic species (Ilex crenata for. microphylla, Acer palmatum, Zingiber mioga, Mercurialis leiocarpa, Osmorhiza aristata, Mecodium wrightii etc.) to explain condition of the habitat and synecological character. The communities were described by concerning their edaphical and syndynamical niche; we discussed their total distribution in Korea. In most forests they are widespread in Korean peninsular and their distribution is primarily determined by artificial plantation and periodical management. The forests consisted of T. nucifera have developed from natural environment element and artificial management. As a result they have very unique characters with the floristic, structural characterization and distribution. Furthermore, we identified that they need to apposite management for sustainability.

• The Journal of Dong Guk Oriental Medicine
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• v.8 no.2
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• pp.69-81
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• 2000

OBJECTIVE Iontophoresis is a kind of medical treatment of penetration of several ion into a body through skin by dirrect current. In oriental medicine for iontophoresis, it has little standard and method to extract the efficient element from herbal medicine. So we studied the quantitative analyzation of extracted Corydalis Tuber. METHODS AND MATERIALS Materials are Corydaline standard and extracted Corydalis Tuber, and the measurement methods of variation by time and pH are HPLC/UV, CG/MS. RESULTS 1. The chromatography and spectrometer of HPLC/UV on extracted Corydalis Tuber has no corydaline. 2. The CG/MS on extracted Corydalis Tuber has no corydaline. CONCLUSION According to the above results, when we extract herbal medicine for iontophoresis, we should consider special nature of the object herbal medicine to take the efficient element. And it needs more syudy on possibility of another element effect with the exception of Corydaline.

• Korean Journal of Environment and Ecology
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• v.21 no.3
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• pp.257-277
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• 2007

We carried out a phytosociological study on pine forests in Korea with the method of Zurich-Montpellier School. We collected the data of 252 $relev{\acute{e}}s$ from 45 sites in the pine forests throughout the Korean Peninsula and its attached islands. The vegetation of the pine forests was classified into one association, three communities and seven subcommunities as follows: A: Quercus mongolica-Pinus densiflora community, A-1: Typical subcommunity, A-2: Vaccinium korean urn subcommunity, A-3 : Rhododendron micranthum subcommunity, B: Quercus serrata-Pinus densiflora community, B-1: Typical subcommunity, B-2: Juniperus rigida subcommunity, B-3: Styrax japonica subcommunity, B-4: Eurya japonica subcommunity, C: Saso-Pinetum densiflorae Yim et al. 1990, and D: Castanopsis cuspidata var. sieboldii-Pinus densiflora community. The former three communities were integrated into the Lindero-Quercion mongolicae Kim 1990 em. 1992. The Castanopsis cuspidata var. sieboldii-Pinus densiflora community remained to be studied in future to determine the association. The communities of Quercus mongolica-Pinus densiflora community was distributed throughout the montane zone in central-northern part of the Korean Peninsula. Quercus serrata-Pinus densiflora community occupied widely in the sub-montane and hilly areas in central and Southern Korean Peninsula. The association of Saso-Pinetum densiflorae was found in Cheju Island. Castanopsis cuspidata var. sieboldii-Pinus densiflora community were distributed in the warm-temperate zone including islands off the south-west coast of the Peninsula.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.3
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• pp.808-814
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• 2010

In this paper we executed a SBL(Short Baseline) underwater acoustic positioning system that is a kind of underwater position estimation system to estimates the 3-dimensional position of ROV(Remotely Operated Vehicle) using hydrophones and DAQ(Data Acquisition) system in the basin which dimensions are $3{\times}3{\times}1.7(m)$. For this experiment, we let 4 hydrophones in different positions of the basin for receiver and 1 hydrophone is fixed on the underwater vehicle for transmitting sensor(pinger). These five hydrophones are communicated with each other to find the 3-D positions of the moving ROV in the basin. The measured signals are collected by DAQ system and the positions of the ROV are plotted by LabView program in real-time. To estimate the position of the ROV we used a trigonometric method. In X and Y plane the estimated data has a small errors but in Z plane the estimated data has large errors so we cannot use this data for position control. One solution of this problem is using depth sensor that implemented of the underwater vehicle. Hereafter, we will test in the ocean using designed SBL system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.323-328
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• 2019

In order to construct 3D geospatial information about the terrain, current measurement using a total station, remote sensing, GNSS(Global Navigation Satellite System) have been used. However, ground survey and GNSS survey have time and economic disadvantages because they have to be surveyed directly in the field. In case of using aerial photographs and satellite images, these methods have the disadvantage that it is difficult to obtain the three-dimensional shape of the terrain. The terrestrial LiDAR can acquire 3D information of X, Y, Z coordinate and shape obtained by scanning innumerable laser pulses at densely spaced intervals on the surface of the object to be observed at high density, and the processing can also be automated. In this study, terrestrial LiDAR was used to analyze slope displacement. Study area slopes were selected and data were acquired using LiDAR in 2016 and 2017. Data processing has been used to generate slope cross section and slope data, and the overlay analysis of the generated data identifies slope displacements within 0.1 m and suggests the possibility of using slope LiDAR on land to manage slopes. If periodic data acquisition and analysis is performed in the future, the method using the terrestrial lidar will contribute to effective risk slope management.

• Journal of Food Hygiene and Safety
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• v.34 no.3
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• pp.263-268
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• 2019

Methanol is a toxic alcohol used in various products such as antifreeze, detergent, disinfectant and industrial solvent. In the human body, methanol is oxidized to formaldehyde and formic acid, which can lead to metabolic acidosis, optic nerve impairment, and death. In this study, the methanol levels in detergents (n=191) and rinse aids (n=13) were analyzed by gas chromatography-headspace-mass spectrometry (GC-HS-MS). Limit of detection was 1.09 mg/kg, accuracy and precision were 91.1-97.9% and <10%, and it was suitable for quantitative analysis. This analysis method was simple and fast with a higher recovery rate than the conventional MFDS (Ministry of Food and Drug Safety) method of diluting the sample in water and putting it in a headspace vial.

• Journal of Wetlands Research
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• v.23 no.3
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• pp.201-212
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• 2021

In wetland protection areas, a phytosociological research was conducted on willow forests, which plays an important ecological and environmental role and is the main material for ecological restoration. A total of 61 relevés were collected according to the Z-M(Zürich-Montpellier) school's method and the characteristics of plant communities and the composition of the species were identified. A total of 9 plant communities including 237 taxa were differentiated. Willow species showing the high r-NCD(relative net contribution degree) value in study areas were Salix koriyanagi, Salix chaenomeloides, Salix triandra subsp. nipponica, Salix gracilistyla and Salix pierotii. Poaceae was the most diverse in species, followed by Asteraceae, Apiaceae, Polygonaceae and Fabaceae. Life form type of willow forests in wetland protection areas was determined to be Th(therophytes)-R₅(non-clonal form)-D₄(clitochores)-e(erect form) type. The naturalized plants and invasive alien species were identified as 24 taxa and 4 taxa, respectively. Naturalized and disturbance indices were 10.1% and 41.4%, respectively. The results of the site-species ordination by Non-Metric Multidimensional Scaling(NMDS), wetland types and altitude gradient were the main ecological factors determining the spatial distribution of plant communities. Diversity index and evenness index were high in mountainous palustrine wetlands with relatively high altitude, and the disturbance index, naturalized index and appearance rate of annual plant were high in riverine and lacustrine wetlands with low altitude.