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http://dx.doi.org/10.7740/kjcs.2011.56.3.244

HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties  

Han, Sang-Jun (Dept. of Agronomy, Korea National Open University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.56, no.3, 2011 , pp. 244-249 More about this Journal
Abstract
A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by $^1H$, $^{13}C$ NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column ($4.6mm{\times}50mm$, $1.8{\mu}m$) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 ${\rightarrow}$ 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.
Keywords
soybean; soyasaponin; HPLC/MS/MS; QQQ;
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1 Tham, D. M., C. D. Gardner, and W. L. Haskell. 1998. Potential health benefits of dietary phytoestrogens: a review of clinical, epidemiological, and mechanistic evidence. J. Clin. Endocrinol. Metab. 83 : 2223-2235.   DOI   ScienceOn
2 Wang, X., X. Zhao, B. Yang, H. Dong, D. Liu, and L. Huang. 2011. A combination of ultrasonic-assisted extraction with RRLC-QQQ method for the determination of artemisin in the chinese herb Artemisia annua L. Phytochemical Analysis. 22(3) : 280-284.   DOI   ScienceOn
3 Yoshiki, Y., J. H. Kim, and K. Okubo. 1994. Saponins conjugated with 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one from Phaseolus coccineus. Phytochemistry. 36(4) : 1009-1012.   DOI   ScienceOn
4 Zhang, W., D. G. Popovich. 2008. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on Hep-G2 cell proliferation and apoptosis. J. Agric. Food Chem. 56 : 2603-2608.   DOI   ScienceOn
5 Zhang, W., S. P. Teng, D. G. Popovich. 2009. Generation of group B woyasaponins I and III by hydrolysis. J. Agric. Food Chem. 57 : 3620-3625.   DOI   ScienceOn
6 Lee, H. P., L. Gourley, S. W. Duffy, J. Esteve, and J. Lee. 1991. Day NE: Dietary effects on breast-cancer risk in Singapore. Lancet. 337(8751) : 1197-1200.   DOI   ScienceOn
7 Perterson, G. 1995. Evaluation of the biochemical targets of genistein in tumor cells. J. Nur. 125(3 Suppl) : 784-789.
8 Ruiz, R. G., K. R. Price, A. E. Arthur, M. E. Rose, M. J. C. Rhodes, R. G. Fenwick. 1996. Effect of soaking and cooking on the saponin content and composition of chickpeas (Cicer arietinum) and lentils (Lens culinaris). J. Agric. Food Chem. 44 : 1526-1530.   DOI   ScienceOn
9 Sakamoto, S., S Kofuji, M. Kuroyanagi, A. Ueno, and S. Sekita. 1992. Saponins from Trifolium repens. Phytochemistry. 31(5) : 1773-1777.   DOI   ScienceOn
10 Song, T. T., S. Hendrich, and P. A. Murphy. 1999. Estrogenic activity of glycitein, a soy isoflavone. J. Agric Food Chem. 47(4) : 1607-1610.   DOI   ScienceOn
11 Kim, S. L., M. A. Berhow, J. T. Kim, I. M. Chung, H. Y. Chi, J. Song, N. K. Park, and J. R. Son. 2006. Composition and content of soyasaponins and their interaction with chemical components in different seed-size soybeans. Korean J. Crop Sci. 51(4) : 340-347.
12 Daveby, Y. D., P. Aman, J. M. Betz, and S. M. Musser. 1998. Effect of storage and extraction on ration of soyasaponin I to 2,3-Dihydro-2,5-dihydroxy-6-methyl-4-pyrone-conjugated soyasaponin I in dehulled peas (Pisum sativum L). J. Sci. Food Agric. 141-146.
13 Kim, Y. H. 2003. Biological activities of soyasaponins and their genetic and environmental variations in soybean. Korean J. Crop Sci. 48(S) : 49-57.
14 Kang, E. Y., S. H. Kim, S. L. Kim, S. H. Seo, E. H. Kim, H. K. Song, J. K. Ahn, and I. M. Chung. 2010. Comparison of soyasapogenol A, B concentrations in soybean seeds and sprouts. Korean J. Crop Sci. 55(2) : 165-176.
15 Chung, H. J., Q. Liu, R. Hoover, T. D. Warkentin, B. Vandenberg. 2008. In vitro starch digestibility, expected glycemic index, and thermal and pasting properties of flours from pea, lentil and chickpea cultivars. J. Food Chem. 118 : 316-321.
16 Lampe, J. W. 2003. isoflavonoid and lignan phytoestrogens as dietary biomarkers. J. Nutr. 133(suppl 3) : 956S-964S.   DOI
17 Adlercreutz, H. and W. Mazur. 1997. Phyto-estrogens and western diseases. Ann. Med. 29 : 95-120.   DOI