• Journal of Microbiology and Biotechnology
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• 제7권6호
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• pp.438-440
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• 1997

A continuous fermentation system employing immobilized cells of Zymomonas mobilis and Saccharomyces cerevisiae was studied for the mass production of ethanol. Ethanol production by cells immobilized with Ca-alginate was better than those by cells immobilized with K-carrageenan. Maximum ethanol production employing a continuous system by cells immobilized with Ca-alginate was 77.5 $g.l^{-1}h^{-1}$ at a dilution rate of 1.85 $h^{-1}$ with 82% conversion rate for Z. mobilis while that was 40.2 $g.l^{-1}h^{-1}$ at a dilution rate of 0.92 $h^{-1}$ with 85% conversion rate for S. cerevisiae. These results suggest that Ca-alginate is a better cell immobilization matrix than K-carrageenan and that immobilized cells of Z. mobilis are more efficient than S. cerevisiae for ethanol production.

• Environmental Engineering Research
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• 제14권1호
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• pp.63-67
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• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• Animal Bioscience
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• 제36권3호
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• pp.429-440
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• 2023

Objective: 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-KETE), a metabolite of arachidonic acid, is a strong candidate signal for placenta separation following calf discharge at delivery. In the present study, the effects of 12-KETE on bovine trophoblast cells were investigated to determine its function in the placentome at delivery. Methods: Bovine trophoblast cells derived from blastocysts were used. They were cocultured with or without fibroblasts derived from bovine placentome and/or bovine uterine epithelial cells. 12-KETE was added to the culture medium. Results: Bovine trophoblast cells contained binucleate cells and strongly expressed caudal type homeobox 2 (CDX-2) genes. Addition of 12-KETE to the trophoblast cell colony without feeder cells or that on a fibroblast monolayer induced rapid exfoliation of the colony. After 12-KETE addition, trophoblast cells emitted strong fluorescence caused by the degradation of dye-quenched collagen, indicating that 12-KETE activated matrix metalloproteinase of the trophoblast cells. Exfoliated cell colonies were stained with YOPRO-1, but not propidium iodide (PI). Moreover, DNA fragmentation and Bcl-2 associated X protein (Bax) gene (apoptosis stimulator) upregulation were observed in exfoliated cells, indicating that 12- KETE induced trophoblast cell apoptosis. These results were consistent with previous in vivo observations; however, even a lower concentration of 12-KETE activated trophoblast protease. Meanwhile, fibroblasts derived from the bovine placentome converted arachidonic acid to 12-KETE. Conclusion: These observations indicate that 12-KETE may serve as a signal for placenta separation at delivery.

• 한국안광학회지
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• 제4권2호
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• pp.51-55
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• 1999

안구운동의 회선점을 중심으로 한 안구운동은 직각좌표의 좌표축 회전변환에 의한 회전행렬 $R_x$, $R_y$, $R_z$로 표현할 수 있으며, 6가지의 눈의 주된 회전 형태인 외-내전, 상-하전, 내-외방 회선 등으로 기술된다. 제1안위에서 제3안위까지의 안구운동은 회전행렬의 조합으로 구성되고, 6가지의 주회전과 8가지의 보조회전으로 6개의 외안근 운동을 표현 할 수 있다. 안구운동에 의한 동공점인 각막정점의 위치를 각각좌표의 회전행렬을 구좌표$(r,{\theta},{\phi})$로 전환하여 9가지로 기술된다.

• Advanced Composite Materials
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• 제18권4호
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• pp.351-364
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• 2009

The thermal stability and elevated temperature mechanical properties of $SiC_P$/Al-11.7Fe-1.3V-1.7Si (Al-11.7Fe-1.3V-1.7Si reinforced with SiC particulates) composites sheets prepared by spray deposition (SD) $\rightarrow$ hot pressing $\rightarrow$ rolling process were investigated. The experimental results showed that the composite possessed high ${\sigma}_b$ (elevated temperature tensile strength), for instance, ${\sigma}_b$ was 315.8 MPa, which was tested at $315^{\circ}C$, meanwhile the figure was 232.6 MPa tested at $400^{\circ}C$, and the elongations were 2.5% and 1.4%, respectively. Furthermore, the composite sheets exhibited excellent thermal stability: the hardness showed no significant decline after annealing at $550^{\circ}C$ for 200 h or at $600^{\circ}C$ for 10 h. The good elevated temperature mechanical properties and excellent thermal stability should mainly be attributed to the formation of spherical ${\alpha}-Al_{12}(Fe,\;V)_3Si$ dispersed phase particulates in the aluminum matrix. Furthermore, the addition of SiC particles into the alloy is another important factor, which the following properties are responsible for. The resultant Si of the reaction between Al matrix and SiC particles diffused into Al matrix can stabilize ${\alpha}-Al_{12}(Fe,\;V)_3Si$ dispersed phase; in addition, the interface (Si layer) improved the wettability of Al/$SiC_P$, hence, elevated the bonding between them. Furthermore, the fine $Al_4C_3$ phase also strengthened the matrix as a dispersion-strengthened phase. Meanwhile, load is transferred from Al matrix to SiC particles, which increased the cooling rate of the melt droplets and improved the solution strengthening and dispersion strengthening.

• 대한수학회보
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• 제48권2호
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• pp.303-314
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• 2011

For Ax = b, it has recently been reported that the convergence of the preconditioned Gauss-Seidel iterative method which uses a matrix of the type P = I + S (${\alpha}$) to perform certain elementary row operations on is faster than the basic Gauss-Seidel method. In this paper, we discuss the adaptive Gauss-Seidel iterative method which uses P = I + S (${\alpha}$) + $\bar{K}({\beta})$ as a preconditioner. We present some comparison theorems, which show the rate of convergence of the new method is faster than the basic method and the method in [7] theoretically. Numerical examples show the effectiveness of our algorithm.

• 호남수학학술지
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• 제31권4호
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• pp.515-527
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• 2009

Let R be a commutative ring with identity. Then we prove $M_n(R)=GL_n(R)$ ${\cup}${$A{\in}M_n(R)\;{\mid}\;detA{\neq}0$ and det $A{\neq}U(R)$}${\cup}Z(M-n(R))$ where U(R) denotes the set of all units of R. In particular, it will be proved that the full matrix ring $M_n(F)$ over a field F is the disjoint union of the general linear group $GL_n(F)$ of degree n over the field F and the set $Z(M_n(F))$ of all zero-divisors of $M_n(F)$. Using the result and universal mapping property we prove that $M_n(F)$ is its total ring of fractions.

• Smart Structures and Systems
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• 제19권2호
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• pp.203-211
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• 2017

This paper provides a numerical solution for multiple confocal elliptic dissimilar cylinders. In the problem, the inner elliptic notch is under the traction free condition. The medium is composed of many confocal elliptic dissimilar cylinders. The transfer matrix method is used to study the continuity condition for the stress and displacement along the interfaces. Two cases, or the infinite matrix case and the finite matrix case, are studied in this paper. In the former case, the remote tension is applied in y- direction. In the latter case, the normal loading is applied along the exterior elliptic contour. For two cases, several numerical results are provided.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1989년도 하계종합학술대회 논문집
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• pp.221-225
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• 1989

Direct calculation algorithm for the elements of A matrix in multimachine power systems with constant impedance loads has been suggested. Generator's rotor parameters need not be determined from the manufacturer's data. We can identify the elements of A matrix into two categories: One is related to only generator parameters, and the other is related to generator parameters, initial values, and $Z_{Bus}$ matrix.

• 호남수학학술지
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• 제27권2호
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• pp.301-315
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• 2005

Let $\{A_u,\;u=0,\;1,\;2,\;{\cdots}\}$ be a sequence of coefficient matrices such that ${\sum}_{u=0}^{\infty}{\parallel}A_u{\parallel}<{\infty}$ and ${\sum}_{u=0}^{\infty}\;A_u{\neq}O_{m{\times}m}$, where for any $m{\times}m(m{\geq}1)$, matrix $A=(a_{ij})$, ${\parallel}A{\parallel}={\sum}_{i=1}^m{\sum}_{j=1}^m{\mid}a_{ij}{\mid}$ and $O_{m{\times}m}$ denotes the $m{\times}m$ zero matrix. In this paper, a functional central limit theorem is derived for a stationary m-dimensional linear process ${\mathbb{X}}_t$ of the form ${\mathbb{X}_t}={\sum}_{u=0}^{\infty}A_u{\mathbb{Z}_{t-u}}$, where $\{\mathbb{Z}_t,\;t=0,\;{\pm}1,\;{\pm}2,\;{\cdots}\}$ is a stationary sequence of linearly positive quadrant dependent m-dimensional random vectors with $E({\mathbb{Z}_t})={{\mathbb{O}}$ and $E{\parallel}{\mathbb{Z}_t}{\parallel}^2<{\infty}$.