• 한국미생물·생명공학회지
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• 제20권6호
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• pp.630-636
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• 1992

토양으로부터 19균주의 메탄올 자화성 효모를 분리하였으며, 이들 중 높은 균체농도와 aldehyde 생산을 보인 균주를 선별 및 동정하여 Hansenula nonfermentant KYP-1으로 명명하였다. Aldehyde 생산은 메탄올 자화성 효모의 균체를 생촉매로 하는 resting cell system에서 행하였으며, 조사된 aldehyde 가운데 acetaldehyde의 생산량이 가장 높았다. 최대의 aldehyde 생산은 40시간 배양한 균체를 생촉매로 이용하였을 때 얻어졌다.

• 한국조리학회지
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• 제9권3호
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• pp.130-140
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• 2003

김치로부터 유산균과 효모를 분리하여 분리균주의 혼합 배양액을 이용하여 제빵 공정의 최적화에 관하여 연구하였다. 김치로부터 젖산박테리아(lactic acid bactria)를 분리하여 제빵 환경에서 생육 조건이 좋은 것으로 나타난 저온 숙성 김치의 젖산균은 Leuconostoc mesenteroides와 Lactobacillus brevis의 2종이 선별되었고, yeast는 Saccharomyces fermentati, Saccharomyces cerevisiae 2종이 선별되었다. 분리된 4종의 균주를 적절하게 혼합배양시 co-work system을 형성하여 발효촉진의 효과를 보여주었다.

• 한국식생활문화학회지
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• 제23권2호
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• pp.228-234
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• 2008

In this study, Astragalus membranaceus powder was added (3, 6, 9%) during yeast bread preparation and its effect on product quality was examined. The results showed that the dough pH increased as the Astragalus membranaceus powder content increased. However, dough volume during fermentation did not differ significantly among the samples. Bread volume decreased (p<0.001) with an increasing amount of Astragalus membranaceus powder. Also, as the Astragalus membranaceus powder content increased, the Hunter’s color ‘L’ value of the crust decreased and the ‘a’ value increased, and for the crumb, the ‘L’ value decreased and the ‘a’ value increased. Textural property analysis indicated that hardness increased with an increasing amount of Astragalus membranaceus powder. However, up to the 6% level, there were no sensory attribute differences among the samples.

• 한국식품영양학회지
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• 제11권6호
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• pp.683-688
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• 1998

This study was conducted to develop optimal composition of flour brew in order to economically utilize flour brew inoculated by lactic acid bacteria as a starter(mother sponge) in bread-making. Two flour brews were prepared ; one with flour and water, the other with flour, water and NaCl. Various nutrients were added to both flour brews and Lactobacilli deMan Rogosa and Sharpe (MRS) broth to investigate the effect of them on growth and activities of Streptococcus thermophilus, Lactobacillus brevis and their mixed culture in flour brews to be tested with incubation at 37$^{\circ}C$. The growth of Streptococcus thermophilus, Lactobacillus brevis and their mixed culture was stimulated by addition of NaCl with 0.85% concentratin and more by mixed culture than by single lactic acid bacteria, resulting in 3 hrs reduction in cultivation and more by mixed culture than by single lactic acid bacteria, resulting in 3 hrs reduction in cultivation time. the addition of 3% glucose to flour brew with NaCl was observed to enhance acid productioni by mixed culture. Yeast extract greatly affected growth and activities of mixed culture of lactic acid bacteria in flour brew with NaCl and its optimum level of this additive in flour brew with NaCl was approximately 1.0%. The optimal composition of flour brew for mixed culture of lactic acid bacteria was suggested as follows; flour 100g, water 300g, NaCl 3.46g, glucose 12.48g, yeast extract 3.46g.

• Journal of Microbiology and Biotechnology
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• 제10권4호
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• pp.482-487
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• 2000

Optimization of submerged culture conditions for the production of exo-biopolymer from Paecilomyces japonica ws studied. Maltose, yeast extract, and potassium phosphate were the most suitable sources of carbon, nitrogen, and inorganic salt, respectively, for both production of the exo-biopolymer and mycelial growth. The optimal culture conditions in a flask culture were pH 5.0, $25^{\circ}C$, and 150 rpm in a medium containing (as in g/l) 30 maltose, 6 yeast extruct, 2 polypeptone, $0.5{\;}K_3HPO_4,{\;}0.2{\;}KH_2PO_4,{\;}0.2{\;}MnSO_4{\cdot}5H_2O,{\;}0.2{\;}MgSO_4{\cdot}7H_2O$. Exo-biopolymer production and mycelial growth in the above suggested medium were significantly increased in a 2.5-1 jar fermentor, where the maximum biopolymer concentration was 8 g/l. The morphological changes of the mycelium in the submerged culture were observed within pH ranges from 4.0 to 9.0; i.e., growth of the filamentous form was optimal at culture pHs of 5.0 and 6.0, whereas pellet was formed at other pHs.

• KSBB Journal
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• 제19권4호
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• pp.291-294
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• 2004

박테리오신의 일종인 leucocin A로 형질전환된 효모가 보이는, B. subtilis에 대한 항균 활성을 파악하기 위하여 형질 전환되지 않은 효모와 형질전환된 효모를 배양하면서 시간별로 채취하여 광학밀도, 건조중량, 총단백질량, 단백질 분해효소 그리고 항균활성을 측정하였다. Leucocin A의 항균활성은 성장양상과 비례하였다. 배양초기에 비해 12시간 배양 후에 항균활성이 3배 이상 증가하는 것으로 나타났고, 이때 B. subtilis의 성장이 70.57% 감소하는 것으로 나타났다. 정지기 이후에는 항균활성이 급속히 감소하였는데, 이는 항균활성을 보이는 leucocin A가 단백질이고, 단백질 분해효소가 정지기 이후 증가한 것에 의한 것으로 사료되었다. 이 연구는 향후 식품산업 등에 사용할 수 있는 bacteriocin의 산업적 생산에 필요한 기초자료를 제공할 수 있을 것이다.

• Archives of Pharmacal Research
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• 제29권3호
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• pp.249-255
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• 2006

Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.

• 한국균학회지
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• 제39권3호
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• pp.227-228
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• 2011

한국 식품연구원에서 분양받은 48종의 효모들의 PD broth 배양 농축물들의 혈전용해활성을 조사하였다. Saccharomyces cerevisiae Y99-7의 PD broth 배양 농축물이 25 mm의 용해환(clear zone)을 보여 가장 높은 혈전용해 활성을 보였다. 또한 선발 효모를 PD broth 배지로 $30^{\circ}C$에서 24 h 배양하였을 때 yeast extract-peptone dextrose 배양농축물 보다 높은 혈전용해활성을 보였다.

• 생명과학회지
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• 제13권4호
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• pp.492-497
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• 2003

액체배양을 이용한 잣버섯(Lentinus lepideus KACC 50120)의 균사체 생육에 관한 결과로는 최적온도는 $25^{\circ}C$이고, 최적 pH는 5.5이었다. 공시배지중에서 최대 균사 생육을 보인 최적배지는 YMG 배지로 조사되었다. YMG 배지를 기본배지로 하여 영양 요구성 실험을 한 결과 탄소원 선발 및 최적 농도에서는 최적 탄소원은 glucose이고 최적 농도는 4.0%(w/v)이며 질소원 선발 및 최적 농도에서는 malt extract와 yeast extract를 혼합하여 사용하였을 때 최대 균사 생육을 보였고, 상기의 두가지 복합질소원을 1:1의 무게비로 혼합하여 1.5%(w/v)농도로 첨가하였을 때 균사생육이 가장 양호하였다.

• Journal of Microbiology and Biotechnology
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• 제6권2호
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• pp.103-109
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• 1996

Mn(II)+succinate decreased the carotenoid formation of the yeast Phaffia rhodozyma, probably by scavenging $O_2$. When duroquinone (DQ), an internal and external $O_2$ generator, was added to medium, P. rhodozyma produced more amount of carotenoids. The increased carotenoid production was destroyed by oxygen radical (OR) scavengers, ascorbate+Cu(II) and dimethylsulfoxide. When sub-lethal concentrations of $H_2O_2$ , an external OR source, and antimycin, an internal OR inducer, were used, the effect of $H_2O_2$ on carotenoid formation and composition was less significant than that of antimycin. Addition of superoxide dismutase, an external OR remover, rescued cells from death caused by the high concentration of DO. In this condition, the yeast culture showed an increase in carotenoid content. Addition of DQ into P. rhodozyma culture in the stationary phase did not increase carotenoid production. Therefore, carotenoid formation was stimulated by internal ORs in the growing yeast. It was probably due to release of catabolite repression on carotenogenesis in the yeast. Aeration was important for carotenoid production but was not as effective as the internal OR producer, DQ.