• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.137-143
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• 2006

The purpose of this study was to prepare a binder containing porcine blood transglutaminase (TGase), thrombin and fibrinogen. Extracted TGase, thrombin and fibrinogen were used alone or mixed with different proportions of their volume (v/v/v) by nine combinations as follows were 0.5:1:15, 0.5:1:20, 0.5:1:25, 1:1:15, 1:1:20, 1:1:25, 1.5:1:15, 1.5:1:20 and 1.5:1:25, respectively. Five ml of each combination were mixed with 0.6 ml of 0.25 M calcium chloride before experiment. After storage at 4C for 0, 1, 2, 3, 4 and 5 weeks, enzyme activity, total plate count, pH value, and SDS-PAGE of TGase, thrombin and fibrinogen were tested and pH value, clotting time and gel strength of the nine combination binders were determined. The results showed that total plate count of thrombin and pH value of TGase were significantly higher (p<0.05) than in other treatments. SDS-PAGE results showed that purified TGase, thrombin and fibrinogen from porcine blood plasma compared with commercial products (Sigma) had the same band patterns and nine different combination binders had no significant effect. Enzymatic activity of TGase and thrombin decreased as storage time increased. Total plate count of TGase, thrombin and fibrinogen and clotting time of the binder increased as storage time increased. The higher amount of fibrinogen in combinations, the stronger the gel strength.

• Korean Journal of Pharmacognosy
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• v.43 no.4
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• pp.297-301
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• 2012

In the course of screening for antioxidant compounds by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH), a total extract of the twigs of Taxillus delavayi (Loranthaceae) was found to show potent radical scavenging activity. Subsequent activity-guided fractionation of the methanolic extract led to the isolation of two phenolic compounds, catechin (1) and galloyl catechin (2), as active principles. Their structures were elucidated by spectroscopic studies. These two compounds showed the significant antioxidative effects on DPPH, and riboflavin- and xanthin-originated superoxide quenching activities. And these two compounds also showed strong nitric oxide radical scavenging effects. In riboflavin- and xanthin-originated superoxide quenching activities and nitric oxide radical scavenging effect, compound 2 showed better antioxidant activities than vitamin C.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.454-464
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• 2019

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, $MgSO_4$ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as $89fg/{\mu}l$, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.

• Journal of Chest Surgery
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• v.55 no.5
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• pp.364-377
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• 2022

An anatomical understanding of the atrial myocardium is crucial for surgeons and interventionists who treat atrial arrhythmias. We reviewed the anatomy of the inter-nodal and intra-atrial conduction systems. The anterior inter-nodal route (#1) arises from the sinus node and runs through the ventral wall of the atrial chambers. The major branch of route #1 approaches the atrioventricular node from the anterior aspect. Other branches of route #1 are Bachmann's bundle and a vestibular branch around the tricuspid valve. The middle inter-nodal route (#2) begins with a broad span of fibers at the sinus venarum and extends to the superior limbus of the oval fossa. The major branch of route #2 joins with the branch of route #1 at the anterior part of the atrioventricular node. The posterior inter-nodal route (#3) is at the terminal crest and gives rise to many branches at the pectinate muscles of the right atrium and then approaches the posterior atrioventricular node after joining with the vestibular branch of route #1. The branches of the left part of Bachmann's bundle and the branches of the second inter-nodal route form a thin myocardial network at the posterior wall of the left atrium. These anatomical structures could be categorized into major routes and side branches. There are 9 or more anatomical circles in the atrial chambers that could be structural sites for macro re-entry. The implications of normal and abnormal structures of the myocardium for the pathogenesis and treatment of atrial arrhythmias are discussed.

• Earthquakes and Structures
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• v.24 no.2
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• pp.81-90
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• 2023

The maximum drifts are important to the seismic evaluation of steel buildings and connections, but the information can hardly be obtained from the post-earthquake field investigation. This research studies the feasibility of using the loss rate of bolt pretension as an earthquake damage predictor. Full-scale tests were made on four steel connections using bolted-web-welded-flange details. One connection was unreinforced (UN), another was reinforced with double shear plates (DS), and the other two used reduced beam sections (RBS). The preinstalled strain gauges were used to control the pretensions and monitor the losses of the high-strength bolts. The results showed that the loss rate of bolt pretension was highly related to the damage of the connections. The pretensions lost up to 10% in all the connections at the yield drifts of 0.5% to 1%. After yielding of the connections, the pretensions lost significantly until fracture occurred. The UN and DS connections failed with a maximum drift of 4 %, and the two RBS connections showed better ductility and failed with a maximum drift of 6%. Under the far-field-type loading protocol, the loss rate grew to 60%. On the contrary, the rate for the specimen under near-fault-type loading protocol was about 40%. The loss rate of bolt pretension is therefore recommended to use as an earthquake damage predictor. Additionally, the 10% and 40% loss rates are recommended to predict the limit states of connection yielding and maximum strength, respectively, and to define the performance levels of serviceability and life-safety for the buildings.

• Journal of the Korea Society of Computer and Information
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• v.29 no.6
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• pp.223-233
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• 2024

This study investigates the impact of personalization recommendation systems on customer loyalty in e-commerce, focusing on the role of information presentation, system interaction, and social community functions. It examines how these elements influence flow state, word of mouth (WOM), and repurchase intention (RPI). Using structural equation modeling (SEM) and data collected from 500 respondents in SPSS and AMOS, the study finds that all three personalization aspects significantly enhance flow state, which, in turn, positively affects WOM and RPI. System interaction directly boosts both WOM and RPI, while information presentation and social community functions influence only one of these loyalty measures. Flow state mediates the relationship between personalization factors and loyalty outcomes. These findings suggest e-commerce platforms should enhance system interaction and embed social community features to foster customer loyalty.

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.143-148
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• 1990

• Journal of Microbiology
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• v.45 no.6
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• pp.479-484
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• 2007

Bacterial communities at 10 cm, 100 cm, and 200 cm depths in a 100-year-old lead-zinc tailing heap were evaluated by constructing 16S rRNA gene libraries. In total, 98 operational taxonomic units (OTUs) were identified from 193 clones at a 3% sequence difference level. The OTU number and species richness decreased with the depth. Species composition was significantly different between the three libraries. Fifty-seven percent of the examined clones were Acidobacteria and 27% belonged to Proteobacteria. Other sequences included Chloroflexi, Firmicutes, Chlamydiae, Actinobacteria, Gemmatimonadetes, Nitrospira, and three unclassified OTUs. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were mainly distributed in the rhizosphere of naturally colonizing plants; however, Deltaproteobacteria, Acidobacteria, and Chloroflexi tended to inhabit the deeper tailings (below the 100 cm-depth).

• Journal of Electrical Engineering and Technology
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• v.10 no.1
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• pp.205-215
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• 2015

Common mode (CM) conducted interference are predicted and compared with experiments in a motor drive system of Electric vehicles in this study. The prediction model considers each part as an equivalent circuit model which is represented by lumped parameters and proposes the parameter extraction method. For the modeling of the inverter, a concentrated and equivalent method is used to process synthetically the CM interference source and the stray capacitance. For the parameter extraction in the power line model, a computation method that combines analytical method and finite element method is used. The modeling of the motor is based on measured date of the impedance and vector fitting technique. It is shown that the parasitic currents and interference voltage in the system can be simulated in the different parts of the prediction model in the conducted frequency range (150 kHz-30 MHz). Experiments have successfully confirmed that the approach is effective.

• Bulletin of the Korean Mathematical Society
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• v.49 no.6
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• pp.1147-1161
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• 2012

In this paper, a coincidence theorem for a compact ${\Re}\mathfrak{C}$-map is proved in an abstract convex space. Several more general coincidence theorems for noncompact ${\Re}\mathfrak{C}$-maps are derived in abstract convex spaces. Some examples are given to illustrate our coincidence theorems. As applications, an alternative theorem concerning the existence of maximal elements, an alternative theorem concerning equilibrium problems and a minimax inequality for three functions are proved in abstract convex spaces.