• KSBB Journal
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• v.21 no.5
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• pp.371-375
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• 2006

Frontal analysis(FA) and Pulsed input method(PIM) have been frequently utilized to measure isotherm of single solute, as well as non-competitive isotherms of two solutes in chromatography(1). FA and PIM were used in this study as complementary methods to measure adsorption isotherms of loxoprofen racemate in HPLC. Prior to FA and PIM experiments, measurements of loxoprofen solubility were made at hexane/ethanol=50/50, 80/20, 95/5(v/v) with acetic acid(0.5%) for adjusting pH. The last composition(95/5) of hexane/ethanol allows us to separate loxoprofen racemate into two forms(retentate, extract). PIM and FA were used to determine the isotherms of re-and ex-loxoprofen.

• Korean Journal of Food Science and Technology
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• v.21 no.3
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• pp.391-398
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• 1989

Triglyceride molecular species In some vegetable oils were analyzed by capillary column gas chromatography and electron impact ionization mass spectrometry utilizing selected ion monitoring. Triglycerides were separated according to their molecular weights and their degrees of unsaturation on $25m{\times}0.25mm$ fused silica open tubular capillary column coated with a phenylmethylsilicone gum stationary phase and in an analysis time less than 13 min. Triglyceride molecular species were identified by analyzing the fragment ions having the same time on the selected ion monitoring profile . The major triglyceride molecular species in each oils were $C_{18:1}.\;C_{18:2}.\;C_{18:2}(OLL:18.3%),\;C_{18:2}.\;C_{18:2}.\;C_{18:2}(LLL;\;14.3%),\;C_{18:0}.\;C_{18:2}.\;C_{18:2}(SLL;\;14.1%),\;C_{16:0}.\;C_{18:2}.\;C_{18:2}(PLL;\;13.2%),\;C_{16:0}.\;C_{18:2}.\;C_{18:1}(PLO;\;11.6%)$ in corn oil, $C_{18:2}.\;C_{18:2}.\;C_{18:2}(LLL;\;18.0%),\;C_{18:1}.\;C_{18:2}.\;C_{18:2}(OLL;\;18.0%),\;C_{16:0}.\;C_{18:2}.\;C_{18:2}(PLL;\;17.1%)$ in safflower oil, $C_{16:0}.\;C_{18:2}.\;C_{18:2}(PLL;\;23.5%),\;C_{16:0}.\;C_{18:2}.\;C_{18:1}(PLO;\;13.8%),\;C_{18:0}.\;C_{18:1}.\;C_{18:1}(SOO;\;13.5%),\;C_{18:1}.\;C_{18:2}.\;C_{18:2}(OLL;\;10.6%)$ in cottonseed oil.

• Korean Chemical Engineering Research
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• v.51 no.4
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• pp.476-481
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• 2013

Ethylbenzene (EB) which has a similar physical properties with p-xylene (pX) was separated from EB/pX mixture by using MFI-type zeolite (TS-1, ZSM-5, and Silicalite-1) coated membranes. The zeolites were synthesized by microwave method to reduce the synthesis time and uniformly formed zeolite particles were coated on the ${\alpha}$-almina tubular support with a thickness of $3-4{\mu}m$. Separation factor and permeation flux of the synthesized zeolite coated membranes were measured to survey the best performance of ethylbenzene separation from different composition of EB/pX mixtures. When the EB/pX mixture of 5:5 molar ratio applied for the separation experiment, it represented the highest separation factor. We also have studied about the effect of the atomic composition of zeolites on the separation performance within the temperature range from 160 to $220^{\circ}C$. TS-1 showed the highest permeation flux of $1,666mol/m^{2*}s^*Pa$ and Silicate-1 showed the highest separation factor of 1.73 at $200^{\circ}C$ respectively.

• Journal of the East Asian Society of Dietary Life
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• v.9 no.4
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• pp.435-441
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• 1999

This study was designed to investigate the effects of roasting temperatures on the antinutritional factors and functional properties of sesame protein isolates. The protein contents a sesame protein isolates (SPI) prepared at roasting temperatures of 5$0^{\circ}C$, 10$0^{\circ}C$, 15$0^{\circ}C$ and 20$0^{\circ}C$ were 65.5%, 66.6%. 68.9% and 64.1%, respectively. Total phenolic compounds, condensed tannin and phytate contents of SPI was increased from 5$0^{\circ}C$ to 20$0^{\circ}C$. From color measurements, higher roasting temperature decreased 'L' and 'b' values significantly, but 'a' value was increased. The bulk density, fat absorption and water absorption of SPI was increased as the temperature of roasting was increased. Sesame protein isolates prepared by roasting at 5$0^{\circ}C$, 10$0^{\circ}C$ and 15$0^{\circ}C$ had higher emulsifying activity than those prepared by roasting at 20$0^{\circ}C$. Foaming capacity of SPI was not changed by roasting up to 10$0^{\circ}C$, but this property was reduced dramatically when roasted at 20$0^{\circ}C$.

• YAKHAK HOEJI
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• v.39 no.5
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• pp.554-559
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• 1995

MT 2511-5 was purified as a inhibitor of phospholipase C(PLC) from culture broth of a Streptmyces sp. NO. 2511-5. It was identified as scopafungin, 36-membered marcrolide by physico-chemical and spectroscopic data. Its $IC_{50}$ was 30.mu.M against phospholipase C and it also showed inhibitory activity against some fungi.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.111-116
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• 2000

Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at $26^{\circ}C$ for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TAlOO.

• Korean Journal of Microbiology
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• v.42 no.4
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• pp.246-251
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• 2006

A lot of mutants which cannot initiate sexual development were screened and several loci including nsdA, nsdB, nsdC, and nsdD were identified in homothallic ascomycetes Aspergillus nidulans. The NSD206, which has nsdC6 allele, showed typical phenotype of NSD (Never in sexual development) mutants. The nsdC gene was cloned by transforming NSDP697 ($nsdC^-$, $pryG^-$) with AMA1-NotI genomic library. The transforming library DNA recovered from several transformants showing wild phenotype carried about 10 kb genomic DNA insert. The DNA sequence of nsdC was analysed using GPS (Genome priming system). The nsdC gene has an open reading frame (ORF) of 1,929 bp encoding a putative polypeptide of 643 amino acids. The NsdC carries $C_2H_2C_2H_2C_2HC$ type zinc finger DNA binding domains in the middle of the polypeptide. A coiled-coil domain at its C terminus were also found. In nsdC6 allele, a single T insertion was occurred between 407-408 bp leading to the frameshift mutation and early termination of translation producing the truncated protein which has only 139 amino acids.

• Microbiology and Biotechnology Letters
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• v.28 no.3
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• pp.139-146
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• 2000

산업적으로 유용한 미생물 유래 셀룰로오스를 생산 이용하기 위해 전국 각지 양조식초의 덧으로부터 세룰로오스의 생산성이 높고 조질의 균일성을 나타내는 균주를 분리하였다 분리균 GS11은 gram 음성이고 간균(0.6$\times$2.2~3.2 $\mu\textrm{m}$)의형태를 하고 있으며 편모를 가지고 있어 운동성을 보였다. 또한 세포내 지방산 조성은 다량의 불포화 지방산 {{{{ {C}_{18:1} }}}}과 포화지반산 {{{{ {C}_{16:0 } }}}}, {{{{ {C}_{14:0 } }}}} 이 대부분을 차지하였고 DNA 염기조성 (G+C) 함량은 58.4% 이였으며 ubiqunone 은 {{{{ { Q}_{10 } }}}}을 갖는 것으로 나타났다 이러한 형태학적 생리.생화학적 특성의 결과에 따라 본 균주는 Acetobacter xylinum GS11으로 동정되었다 A. xylinum, GS11 의배양기간 동안 셀룰로오스 생산성을 검토하고자 250mL 삼각플라스크에 균주를 접종하여 $30^{\circ}C$에서 12일간 정치배양하였다 그결과 기질인 glucose의 소비는 접종 후 급소하게 감소하여 셀룰로오스 생산에 이용되었으며 셀룰오스의 생산은 배양 9일 경에 2.8g/l로 최대의 생산량을 나타냈다.

• Korean Journal of Microbiology
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• v.47 no.2
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• pp.130-136
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• 2011

Five strains of Actinomyces were isolated from freshwater sponges, Baikalospongia and Lubomirskia, in Lake Baikal. By 16S rRNA sequencing, isolates were identified as Streptomyces griseoplanus, S. halstedii, S. violascens, S. flavovirens, and S. microflavus. Isolates had different characteristics of growth temperature, carbon utilization, enzyme activity, and cellular fatty acid composition. Optimum growth conditions of isolates were $30-37^{\circ}C$, pH 8-9, and 0-1.5% salt concentrations. Major fatty acid compositions were anteiso-$C_{15:0}$, iso-$C_{15:0}$, and iso-$C_{16:0}$. Strain ATS-BA-19 had DNase and chitinase activities and strain ATS-BA-16 had cellulase and protease activities. Colonies of strain ATS-BA-15 and ATS-BA-19 made inhibition zone of Pseudomonas aeruginosa.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.196-196
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• 2011

팔라듐-구리 합금 분리막은 세륨산화물로 전처리된 다공성 니켈 지지체 위에 마그네트론 스퍼터 공정과 구리리플로우 공정에 의해 제조되었다. 스퍼터 공정은 얇고 치밀한 팔라듐 합금 분리막 증착을 위해 아주 효과적이다. 본 연구에서는 고온 스퍼터 공정에 의해 증착된 팔라듐 상부에 유동성과 열적확산이 우수한 구리를 코팅한 후, 반도체 분야에서 기가 패턴 매립시 사용하는 구리리플로우 공정을 도입하였다. 구리리플로우 공정은 치밀하고 미세기공이 존재하지 않는 표면을 구현하고 무한대의 수소 투과도를 가능하게 한다. 이로써 마그네트론 스퍼터에 의해 $200^{\circ}C$에서 팔라듐과 구리를 순차적으로 코팅한 후, $700^{\circ}C$에서 2시간 구리리플로우 공정을 실시하여 $7.5{\mu}m$ 두께의 팔라듐-구리 합금 분리막이 제조되었다. 세륨산화물(CeO2)은 고온에서 장시간 운전하는 동안 다공성 니켈 지지체의 금속성분이 팔라듐 합금층으로 확산하는 금속의 확산 문제를 개선하고자 지지체와 코팅층 사이에 확산방지막으로 도입되었으며, 균일한 스퍼터 증착을 위해 평탄한 표면의 지지체를 구현하였다. 투과도 테스트는 100-400kPa 의 압력차, 673-773K 의 온도 조건에서 순수한 수소가스로 실시하였다. 표면 미세기공이 없는 치밀한 팔라듐-구리 합금 분리막은 혼합가스에서 질소의 투과 없이 수소만을 투과하는 무한대의 우수한 분리도를 나타내었으며, 상용온도 $500^{\circ}C$에서 12.6ml/$cm^2{\cdot}min{\cdot}atm$의 수소 투과 능력을 보였다. 본 연구에 의해 제조된 팔라듐-구리 합금 분리막은 표면 미세기공이 없는 치밀한 분리막 제조를 가능하게 하였으며 열팽창계수가 팔라듐과 매우 비슷한 세륨산화물($CeO_2$)로 인해 지지체층과 코팅층과의 접합력이 향상되고 수소취성에 강하고 높은 열적 안정성을 갖는다.