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Characterization of Actinomyces Isolated from Freshwater Sponges in Lake Baikal  

Jung, You-Jung (Department of Environmental Science, Kangwon National University)
Joung, Yo-Chan (Department of Environmental Science, Kangwon National University)
Ahn, Tae-Seok (Department of Environmental Science, Kangwon National University)
Publication Information
Korean Journal of Microbiology / v.47, no.2, 2011 , pp. 130-136 More about this Journal
Abstract
Five strains of Actinomyces were isolated from freshwater sponges, Baikalospongia and Lubomirskia, in Lake Baikal. By 16S rRNA sequencing, isolates were identified as Streptomyces griseoplanus, S. halstedii, S. violascens, S. flavovirens, and S. microflavus. Isolates had different characteristics of growth temperature, carbon utilization, enzyme activity, and cellular fatty acid composition. Optimum growth conditions of isolates were $30-37^{\circ}C$, pH 8-9, and 0-1.5% salt concentrations. Major fatty acid compositions were anteiso-$C_{15:0}$, iso-$C_{15:0}$, and iso-$C_{16:0}$. Strain ATS-BA-19 had DNase and chitinase activities and strain ATS-BA-16 had cellulase and protease activities. Colonies of strain ATS-BA-15 and ATS-BA-19 made inhibition zone of Pseudomonas aeruginosa.
Keywords
Actinomycetes; fresh water; lake Baikal; sponge;
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