• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1428-1440
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• 2017

Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production ($110.28{\mu}g/l$), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and $24{\mu}g$ per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.5
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• pp.736-742
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• 2017

Objective: Experiments were conducted to clone the sequence of Wild Argali short palate, lung and nasal epithelium clone 1 (SPLUNC1) cDNA, and to lay the foundation for further study the biological function of Wild Argali SPLUNC1. Methods: The complete sequence of Wild Argali SPLUNC1 cDNA was generated by rapid amplification of cDNA ends. The entire coding sequence was inserted into the pPIC9K vector and expressed in Pichia pastoris (P. pastoris) GS115. The recombinant SPLUNC1 protein was detected by Western blot and purified by $Ni^{2+}$ chelate affinity chromatography. The test of effect of the protein on Mycoplasma ovipneumoniae (MO) was performed with real-time polymerase chain reaction. Results: The Wild Argali SPLUNC1 cDNA was 1,076 bp with an open reading frame of 768 bp, which encoded a 26.49 kDa protein composed of 255 amino acids. Its amino acid sequence shared 98.4%, 96.9%, 94.5%, 90.2%, 80.8%, 78.4%, 78.3%, 72.5%, 72.3%, 68.8% identity with those of SPLUNC1 cDNA from Ovis aries (accession no. NP_001288334.1), Capra hircus (accession no. XP_005688516.1), Pantholops hodgsonii (accession no. XP_005979709.1), Bos taurus (accession no. NP_776851.1), Felis catus (accession no. XP_006929910.1), Homo sapiens (accession no. NP_001230122.1), Sus scrofa (accession no. NP_001005727.1), Chinchilla lanigera (accession no. NP_001269294.1), Mus musculus (accession no. NP_035256.2), and Rattus norvegicus (accession no. NP_742028.1), respectively. The recombinant protein corresponded to the expected molecular mass of 25.47 kDa as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was detected in the supernatant of P. pastoris, and it could be purified. The results from the test of inhibition effect of argali recombinant SPLUNC1 protein on MO showed that the product could inhibit MO very well (p<0.01). Conclusion: The amino acid sequence of Wild Argali SPLUNC1 was different from other organisms. The recombinant SPLUNC1 protein has good biological activity.

• Korean Journal of Food Science and Technology
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• v.28 no.3
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• pp.580-586
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• 1996

Gelatinization behavior and morphology of epichlorohydrin-crosslinked potato starches (XPs) were investigated. Native potato starch showed a very steep single stage swlling pattern, but crosslinked starches showed various patterns with the degree of crosslinking. Swelling power, solubility and light transmittance were reduced drastically as the degree of crosslinking increased. Brabender initial pasting temperature and peak temperature of crosslinked starches increased because the crosslinking reinforces the intermolecular net work of the starches. Although the swelling of the potato starch granule was inhibited by crosslinking as compared to that of the native one, Brabender peak viscosities (6.5% w/v, db) were on the order of 2,500 units for the native potato starch, 3,700 for the XP with 2.300 anhydroglucose units per crosslinking (AGU/CL) and 3,400 for the XP with 2,100 AGU/CL, due to the decreased breakdown of the swollen granule resulting from the resistance to heat and shear. The XP with 1,900 AGU/CL, however, did not show the peak viscosity and the viscosity was on the order of 500 units because of the excessive unhibition of the swelling. Unlike the native potato starch, 6.5%(m/v, db) pastes of the crosslinked potato starches could form gels, which could be predicted from the Brabender setback and consistency index. When the degree of crosslinking is low, random contraction and radial swelling of the granule was possible. As the degree of crosslinking increased, morphological change became similar to the single dimensional tangential swelling observed from the lenticular wheat starch. These morphological change during heating in excess water explained the gelatinization behaviors of crosslinked starches tested.

• Journal of Korea Water Resources Association
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• v.48 no.9
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• pp.729-741
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• 2015

This study examined the applicability of MBLRP (Modified Bartlett-Lewis Rectangular Pulse) rainfall generation model for an urban flood simulation which is a type of Poisson cluster rainfall generation model. This study constructed XP-SWMM model for Namgajwa area of Hongjecheon basin, which is a two-dimensional pipe network-surface flood simulation program and computed a flood discharge and a flooded area with input data of synthetic rainfall time series of 200 years that were generated by the MBLRP model. This study compared the data of flood with synthetic rainfall and flood with corresponding values which were based on design rainfall. The results showed that the flooded area computed with MBLRP model was somewhat smaller than the corresponding values on the basis of the design. A degree of underestimation was from 8% (5 year) to 34% (200 year) and the degree of underestimation increased as a return period increased. This study is meaningful in that it proposes methodology that enables quantifiability of uncertain variables which are related to a flooding through Monte Carlo analysis of urban flooding simulation and applicability and limitations thereof.

• Journal of Wetlands Research
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• v.15 no.3
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• pp.413-422
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• 2013

In recent, the rainfall is showing different properties in space and time but the ground rain gauge only can observe rainfall at a point. This means the ground rain gauge has the limitations in spatial and temporal resolutions to measure rainfall and so there is a need to utilize radar rainfall which can consider spatial distribution of rainfall This study tried to apply radar rainfall for runoff simulation on an urban drainage system. The study area is Guro-gu, Seoul and we divided study area into subbasins based on rain gauge network of AWS(Automatic Weather station). Then the radar rainfalls were adjusted using rainfall data of rain gauge stations the areal rainfalls were obtained. The runoffs were simulated by using XP-SWMM model in subbasins of an urban drainage system. As the results, the adjusted radar rainfalls were underestimated in the range of 60 to 95% of rain gauge rainfalls and so the simulated runoffs from the adjusted radar and gauge rainfalls also showed the differences. The runoff peak time from radar rainfall was occurred more fast than that from gauge rainfall.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.486-490
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• 2017

도심지에서의 침수피해는 이상홍수 및 국지성 호우 시 우수관거 시설기준 미달, 펌프장 등 배수시설이 설치되지 않아 하천의 계획홍수위보다 제내지의 지반고가 낮은 저지대 지역에서 많이 발생하고 있다. 특히, 내수침수의 경우는 외수에 따른 범람보다는 국민의 재산과 인명피해에 직접적인 영향을 미치므로 침수피해 위험도가 높은 지역의 주민에게 그 지역의 침수빈도와 범위를 인지시키고 사전대응 능력을 향상시킬 필요가 있다. 따라서 연구의 목적으로 매년 피해가 발생한 이력이 있는 위험지구에 대해 전국단위 시군구별 침수피해 지도를 작성하여 침수심 산정과 피해액 예측할 수 있는 기초자료로 활용하고, 주민들의 신속한 대처를 통해 그들의 생명과 재산을 보호하여 재난 안전 국가 이미지 제고에 기여하고자 한다. 본 연구에서는 도심지 유출모형인 XP-SWMM을 활용하여 내수재해 위험요인에 대한 전국을 해석하는 것에 한계가 있어 풍수해저감종합계획에 수록된 XP-SWMM모의 분석 결과 값을 활용하고자 하였다. 기 수립된 전국 풍수해저감종합계획의 과거 피해 자료를 바탕으로 이상 집중호우나 태풍의 내습 시 풍수해 피해 발생 가능성이 제일 높은 지역을 연구범위 대상지역으로 선정하였다. 그 중 풍수해의 주요 원인으로서 태풍, 집중호우 및 해일로 인한 피해발생 빈도가 높은 지역이면서 하천재해 및 내수침수 피해가 많은 경기도 동두천시를 연구대상 지역으로 선정하였으며, 대상지 유역 현황과 지형정보 및 빈도별 침수심을 조사하였다. 수록된 내용에 따르면 경기도 동두천시는 우수관망의 밀도가 높은 4개 위험지구를 내수재해 발생가능성 지역으로 선정하여 10년, 20년, 30년, 50년, 100년, 200년 6개 빈도에 대해 XP-SWMM 모의를 실시하였다. 이와 같이 수록된 각 빈도에 대한 모의 결과 값을 GIS기술을 이용하여 디지털화 하고 부가적인 분석을 위한 GIS데이터화 하는 내삽법을 선정하여 침수면적 및 침수심을 산출하였다. 그러나 면적비교를 통해 모의 결과 값을 디지털화 하는 과정에서 많은 오차가 발생되는 것을 확인하였고, 이를 보완하기 위해 좌표보정 자동화 프로그램을 개발하여 이러한 문제점을 제거하여 신뢰도를 향상시켰다. 이렇게 계산된 연구 대상지역의 침수심과 침수면적을 활용하여 지도제작 표준 지침서 및 가이드라인을 제시하여 한국형 호우피해 지도제작 기술개발에 기여하고, 비구조적 대책으로서 이상홍수에 대한 위험도를 파악하여 지역별 도심침수 방지를 위한 대비체계를 구축하는 등 위험지역에 대한 사전분석 및 활용에 기초자료로 도움이 되고자 한다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.561-567
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• 2018

This study was carried out to improve the ride quality of high-speed electric multiple unit. Through dynamic analysis of the HEMU-430X, the range of the equivalent conicity with a critical speed of 300 km/h was between 0.05 and 0.25. The initial adopted wheel profile of HEMU-430X was S1002. The equivalent conicity of S1002 with the mileage of more than 40,000 km was about 0.033 and it was confirmed that XP55 is more suitable for stable operation because XP55 has the equivalent conicity of over 0.061. In order to improve ride quality of high-speed electric multiple unit, the change of installation angle of the yaw damper was suggested from $7.35^{\circ}$ to $0^{\circ}$. From sensitivity analysis and optimization, the air spring lateral and vertical stiffness was suggested to be reduced by 30% and the secondary vertical and lateral damper damping coefficient was increased by 50%. By applying this, it was expected that the car body acceleration could be improved by about 20% on average. The HEMU-430X's yaw damper installation angle was changed to $0^{\circ}$ and the damping coefficient of the lateral damper was increased by 30%. When the test run was carried out at the speed of 300 km/h on the Kyungbu high-speed line, the vehicle lateral acceleration had improved by 34.3%. The effect of additional improvement measures proposed in this paper will be tested in the on track test. The riding quality improvement process used in this study can be used to solve ride quality problems that can occur in commercial operation of high-speed electric multiple unit in the future.

• Journal of Korean Society of Disaster and Security
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• v.16 no.1
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• pp.13-22
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• 2023

The importance of preemptive flood preparation is growing as the importance of preparing for climate change increases due to record heavy rains in the Seoul metropolitan area in August 2022. Although it is responding to flood control through reservoirs and sediment sites, the government is preparing excellent spill reduction measures through a preliminary consultation system for Low Impact Development (LID). In this study, the depth of flooding was simulated when LID technologies were applied to the Sillim 2-drain region in Dorimcheon Stream basin, an urban stream, using XP-SWMM, a two-dimensional model. In addition, the analysis and applicability of the effect of reducing rainfall runoff for the largest rainfall in a day were reviewed, and it was judged to be effective as a method of reducing flooding in urban areas. Although there is a limitation in which the reduction effect is overestimated, it is thought that the LID technologies can be a significant countermeasure as a countermeasure for small-scale flooded areas where some flooding occurs after structural flooding measures are established.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.624-624
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• 2013

본 연구에서는 전자빔 조사를 이용하여 대기 조건에서 실리카 나노입자를 제조하였다. 제조된 실리카 나노입자는 FT-IR을 통해 전구체가 전자빔에 의하여 분해되었음을 확인하였고 또한 XPS를 통해 Si 2P binding energy가 확인되었다. 본 연구에서 제조된 나노입자의 평균 지름은 각각 210 nm와 73 nm로 나타났고, 입자의 평균지름은 전구체 증기의 전자빔 반응기내 체류시간 조절에 따라 제어된다.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.284-284
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• 2013

최근연구에 의하면 가시광선에 활성을 띄는 TiO2 개발은 중요한 연구 과제이다. 우리는 몇 가지 아민류를 이용해 수열합성법으로 TiO2에 질소를 도핑 함으로써 그 가시광선 활성을 연구하였다. 그 특성연구로서 XRD, SEM, TEM을 통해 구조와 morphology을 나타내었으며, EDX와 XPS를 통해 질소도핑 유무를 밝혔다. UV-Visible 분광기를 통해서 시료 각각의 가시광선영역에서의 흡수 유무를 확인 하였으며, 마지막으로 가시광선 영역에서 광촉매 활성을 알아보기 위해서, 광원으로 Xe-lamp를 이용, methylene blue를 분해해 그 농도를 분석하여 가시광활성을 비교 분석하였다.