• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.322-328
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• 2013

In this study, we show the expression and purification of the human recombinant farnesoid X receptor (FXR)- ligand binding domain (LBD) protein in E. coli using a double cistronic vector, pACYCDuet-1, as a soluble form. We describe here the expression and characterization of a biologically active $FXR-LBD_{(248-476)}$. When expressed in the influence of bacterial promoters ($P_{T7}$ and $P_{Tac}$) of the single cistronic expression vectors, the human recombinant $FXR-LBD_{(248-476)}$ was found to be totally insoluble. However, by using a double cistronic expression vector, we were able to obtain the human recombinant $FXR-LBD_{(248-476)}$ in a soluble form. To allow for biological activities, we have subcloned into the pACYCDuet-1 vector, expressed in E. coli cells at some optimized conditions, and purified and characterized the human recombinant active $FXR-LBD_{(248-476)}$ proteins using the fluorescence polarization assay. This suggests that the expression of FXR-LBD in a double cistronic vector improves its solubility and probably assists its correct folding for the biologically active form of the proteins. We suggest that this may represent a new approach to high expression of other nuclear receptors and may be useful as well for other classes of heterodimeric protein partners.

• Bulletin of the Korean Mathematical Society
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• v.26 no.2
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• pp.139-149
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• 1989

pp.Hartman and G. Stampacchia [6] proved the following theorem in 1966: If f:X.rarw. $R^{n}$ is a continuous map on a compact convex subset X of $R^{n}$ , then there exists $x_{0}$ ..mem.X such that $x_{0}$ , $x_{0}$ -x>.geq.0 for all x.mem.X. This remarkable result has been investigated and generalized by F.E. Browder [1], [2], W. Takahashi [9], S. Park [8] and others. For example, Browder extended this theorem to a map f defined on a compact convex subser X of a topological vector space E into the dual space $E^{*}$; see [2, Theorem 2]. And Takahashi extended Browder's theorem to closed convex sets in topological vector space; see [9, Theorem 3]. In Section 2, we obtain some variational inequalities, especially, generalizations of Browder's and Takahashi's theorems. The generalization of Browder's is an earlier result of the first author [8]. In Section 3, using Theorem 1, we improve and extend some known fixed pint theorems. Theorems 4 and 8 improve Takahashi's results [9, Theorems 5 and 9], respectively. Theorem 4 extends the first author's fixed point theorem [8, Theorem 8] (Theorem 5 in this paper) which is a generalization of Browder [1, Theroem 1]. Theorem 8 extends Theorem 9 which is a generalization of Browder [2, Theorem 3]. Finally, in Section 4, we obtain variational inequalities for multivalued maps by using Theorem 1. We improve Takahashi's results [9, Theorems 21 and 22] which are generalization of Browder [2, Theorem 6] and the Kakutani fixed point theorem [7], respectively.ani fixed point theorem [7], respectively.

• Honam Mathematical Journal
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• v.30 no.2
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• pp.219-225
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• 2008

In this paper, we obtain the general solution and the stability of the 2-dimensional vector variable quadratic functional equation f( x + y, z - w) + f(x - y, z + w) = 2f(x, z ) + 2f(y, ${\omega}$). The quadratic form f( x, y) = $ax^2$ + $by^2$ without cross product terms is a solution of the above functional equation.

• Journal of the Chungcheong Mathematical Society
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• v.17 no.1
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• pp.57-75
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• 2004

The aim of this paper is to solve the general solution of a quadratic functional equation f(x + 2y) + 2f(x - y) = f(x - 2y) + 2f(x + y) in the class of functions between real vector spaces and to obtain the generalized Hyers-Ulam stability problem for the equation.

• Korean Journal of Mathematics
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• v.18 no.4
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• pp.387-394
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• 2010

In this paper, we study the generalized Randers change $^*L(x,y)=L(x,y)+b_i(x,y)y^i$ from the Brewald metric L and the h-vector $b_i$. And in search for a non-Berwald Landsberg metric, we obtain the conditions on $b_i(x,y)$ under which $^*L$ is a Landsberg metric.

• Bulletin of the Korean Mathematical Society
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• v.42 no.1
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• pp.133-148
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• 2005

In this paper, we obtain the general solution of a gen-eralized quadratic and additive type functional equation f(x + ay) + af(x - y) = f(x - ay) + af(x + y) for any integer a with a $\neq$ -1. 0, 1 in the class of functions between real vector spaces and investigate the generalized Hyers- Ulam stability problem for the equation.

• Korean Journal of Mathematics
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• v.7 no.1
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• pp.45-51
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• 1999

Let X and Y be topological vector spaces. For a sequence {$T_j$} of bounded operators from X into Y the $c_0$-multiplier convergence of ${\sum}T_j$ is an invariant on topologies which are stronger (need not strictly) than the topology of pointwise convergence on X but are weaker (need not strictly) than the topology of uniform convergence on bounded subsets of X.

• Journal of the Korean Mathematical Society
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• v.34 no.3
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• pp.553-567
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• 1997

This paper is concerned with the control problem $$ \dot{x}(t) = F(x) + u(t)G(x), t \in [0,T], x(0) = 0, $$ where F and G are smooth vector fields on $R^n$, and the admissible controls u satisfy the constraint $$\mid$u(t)$\mid$ \leq 1$. We provide the sufficient condition that the bang-bang trajectories having different switching orders intersect.

• Journal of the Chungcheong Mathematical Society
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• v.31 no.4
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• pp.397-409
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• 2018

In this paper, we investigate the generalized Hyers-Ulam stability of the functional equation $$f\({\sum\limits_{i=1}^{n}}x_i\)+{\sum\limits_{1{\leq}i<j{\leq}n}}f(x_i-x_j)-n{\sum\limits_{i=1}^{n}f(x_i)=0$$ for integer values of n such that $n{\geq}2$, where f is a mapping from a vector space V to a Banach space Y.

• Journal of the Chungcheong Mathematical Society
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• v.23 no.3
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• pp.495-510
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• 2010

An Einstein's connection which takes the form (2.23) is called a $^*g$-ME-connection and the corresponding vector is called a $^*g$-ME-vector. The $^*g$-ME-manifold is a generalized n-dimensional Riemannian manifold $X_n$ on which the differential geometric structure is imposed by the unified field tensor $^*g^{{\lambda}{\nu}}$, satisfying certain conditions, through the $^*g$-ME-connection and we denote it by $^*g-MEX_n$. The purpose of this paper is to derive a general representation and a special representation of the $^*g$-ME-vector in $^*g-MEX_n$.