• Title/Summary/Keyword: X-ray Micro CT

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A Study on the Usefulness of 3D Imaging in Micro-CT for Observing the Microstructure of Mice (흰쥐 미세구조 관찰을 위한 Micro-CT 3D 영상의 유용성에 관한 연구)

  • Lee, Sang-Ho;Lee, Jong-Seok;Lim, Cheong-Hwan;Jung, Hong-Ryang;Chai, Kyu-Yun;Han, Beom-Hee;Han, Sang-Hyun;Hong, Dong-Hee;Han, Tae-Jong;Park, Sung-Hoon;Mo, Eun-Hee;Jun, Hong-Young
    • Journal of Digital Convergence
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    • v.12 no.3
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    • pp.367-375
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    • 2014
  • In this thesis we observe microvascular structure in mice by using micro-computed tomography (CT), which is high-resolution X-ray imaging equipment that can acquire Real-time dynamic image, and it aims to investigate the usefulness of micro-CT developed by Institute for Radiological Imaging Science Wonkwang University School of Medicine. After acquiring the systemic images of rats, contrast-enhanced 3D images of vascular structures could be acquired by using Maximum Intensity Projection (MIP) and Volume Rending Technique (VRT), This was divided into each vascular system of head, abdomen and heart and systemic vascular system.

Comparison of limited- and large-volume cone-beam computed tomography using a small voxel size for detecting isthmuses in mandibular molars

  • de Souza Tolentino, Elen;Andres Amoroso-Silva, Pablo;Alcalde, Murilo Priori;Yamashita, Fernanda Chiguti;Iwaki, Lilian Cristina Vessoni;Rubira-Bullen, Izabel Regina Fischer;Duarte, Marco Antonio Hungaro
    • Imaging Science in Dentistry
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    • v.51 no.1
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    • pp.27-34
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    • 2021
  • Purpose: This study was performed to compare the ability of limited- and large-volume cone-beam computed tomography (CBCT) to display isthmuses in the apical root canals of mandibular molars. Materials and Methods: Forty human mandibular first molars with isthmuses in the apical 3 mm of mesial roots were scanned by micro-computed tomography (micro-CT), and their thickness, area, and length were recorded. The samples were examined using 2 CBCT systems, using the smallest voxels and field of view available for each device. The Mann-Whitney, Friedman, and Dunn multiple comparison tests were performed (α=0.05). Results: The 3D Accuitomo 170 and i-Cat devices detected 77.5% and 75.0% of isthmuses, respectively (P>0.05). For length measurements, there were significant differences between micro-CT and both 3D Accuitomo 170 and i-Cat(P<0.05). Conclusion: Both CBCT systems performed similarly and did not detect isthmuses in the apical third in some cases. CBCT still does not equal the performance of micro-CT in isthmus detection, but it is nonetheless a valuable tool in endodontic practice.

How do imaging protocols affect the assessment of root-end fillings?

  • Fernanda Ferrari Esteves Torres;Reinhilde Jacobs;Mostafa EzEldeen;Karla de Faria-Vasconcelos;Juliane Maria Guerreiro-Tanomaru;Bernardo Camargo dos Santos;Mario Tanomaru-Filho
    • Restorative Dentistry and Endodontics
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    • v.47 no.1
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    • pp.2.1-2.11
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    • 2022
  • Objectives: This study investigated the impact of micro-computed tomography (micro-CT)-based voxel size on the analysis of material/dentin interface voids and thickness of different endodontic cements. Materials and Methods: Following root-end resection and apical preparation, maxillary premolars were filled with mineral trioxide aggregate (MTA), Biodentine, and intermediate restorative material (IRM) (n = 24). The samples were scanned using micro-CT (SkyScan 1272; Bruker) and the cement/dentin interface and thickness of materials were evaluated at voxel sizes of 5, 10, and 20 ㎛. Analysis of variance and the Tukey test were conducted, and the degree of agreement between different voxel sizes was evaluated using the Bland and Altman method (p < 0.05). Results: All materials showed an increase in thickness from 5 to 10 and 20 ㎛ (p < 0.05). When evaluating the interface voids, materials were similar at 5 ㎛ (p > 0.05), while at 10 and 20 ㎛ Biodentine showed the lowest percentage of voids (p < 0.05). A decrease in the interface voids was observed for MTA and IRM at 20 ㎛, while Biodentine showed differences among all voxel sizes (p < 0.05). The Bland-Altman plots for comparisons among voxel sizes showed the largest deviations when comparing images between 5 and 20 ㎛. Conclusions: Voxel size had an impact on the micro-CT evaluation of thickness and interface voids of endodontic materials. All cements exhibited an increase in thickness and a decrease in the void percentage as the voxel size increased, especially when evaluating images at 20 ㎛.

Accuracy verification of dental cone-beam computed tomography of mandibular incisor root canals and assessment of its morphology and aging-related changes

  • Katsuyuki Aoki;Masamitsu Serikawa;Takuya Harada;Akinobu Usami
    • Anatomy and Cell Biology
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    • v.56 no.2
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    • pp.185-190
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    • 2023
  • The root canal morphology undergoes aging-related changes, and relevant quantitative analyses have not yet been reported. We compared the cone beam computed tomography (CBCT) and micro-computed tomography (microCT) scans of extracted mandibular incisors to check the accuracy of morphological measurements. Thereafter, the root canal morphology and aging-related changes in the mandibular incisors of Japanese individuals were assessed using CBCT. Six extracted teeth were fixed in a phantom head and imaged using CBCT and micro-CT. The correlation between the findings of the two imaging modalities was examined. Further, CBCT reconstructed images of the mandibular incisors of 81 individuals were observed. Age-related changes of the root canals were compared between participants aged <30 years and those aged ≥30 years. The CBCT and micro-CT findings regarding the root canals of the extracted teeth coincided in 94.4% of the cases. Mandibular incisors exhibiting two root canals in either cross-section accounted for 9.9% of central incisors and 12.4% of lateral incisors. Mandibular central incisors with two root canals were observed in two (6.3%) individuals aged <30 years and six (12.2%) aged ≥30 years. Mandibular lateral incisors with two root canals were observed in one (3.1%) individual aged <30 years and nine (18.4%) aged ≥30 years. CBCT allows accurate evaluation of complex root canal morphologies and is useful for endodontic preoperative assessment. Mandibular incisors have more frequent occurrence of two root canals with aging.

Microvascular Contrast Image in Portal Veins of Rat using Micro-CT (마이크로 CT를 이용한 BALB/C(흰쥐) 간문맥의 미세혈관 조영 영상)

  • Lee, Sang-Ho;Lim, Cheong-Hwan;Jung, Hong-Rayng;Han, Beom-Hee;Mo, Eun-Hee;Chai, Kyu-Yun
    • The Journal of the Korea Contents Association
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    • v.10 no.9
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    • pp.259-266
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    • 2010
  • The study focuses on the value of Micro CT, a high resolution X-ray imaging device, by using it on rats to observe the overall portal vein image of the liver and the microvasculature of each lobes, visualize the 4 segmental lobes and acquire 3D image of the microvasculature through the reconstruction of sectional image data. Less of the damage to liver of the 5 mice, the device was able to separate the liver into 4 segmental lobes and displayed the 4 portal vein microvasculature in 2D. By using the 3D MIP technique, observation of the whole portal vein system microvasculature in 3D image was made possible along with each of the portal vein segment's branches until the 6th branch. Measured the size of 6branch, the average was measured at 1branch : $0.51mm{\pm}0.08$, 2 branch : $0.32mm{\pm}0.12$, 3 branch : $0.23mm{\pm}0.11$, 4 branch : $0.19mm{\pm}0.08$, 5 branch : $0.13mm{\pm}0.06$, 6 branch : $70.5{\mu}m{\pm}14.1$. The 3D image and the images of the microvasculatures in the result of study proved that the Micro-CT can be considered many useful device in obtaining high resolution images.

Suggestion of the morphological characteristic parameters according to the Lee's physical constitution theory using X-ray computed tomography (X선 CT화상을 이용한 사상체질의 형태학적 특징 파라메타의 제안)

  • Jo, Bong-Kwan;Bae, Jong-Il;Ko, Byung-Hee;Saito, Masao
    • Proceedings of the KOSOMBE Conference
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    • v.1993 no.11
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    • pp.13-16
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    • 1993
  • In this paper the morphological characteristic parameters according to the Lee's physical constitution theory are suggested using the X-ray computed tomography. The Lee's physical constitution theory is classify into 4 physical constitutional types by the relative degree of visceral function: those are macro-negative(strong liver- weak lung), micro-negative(strong kidney- weak spleen), micro-positive(strong spleen- weak kidney) and macro-positive(string lung- weak liver). The morphological characteristic parameters are the following. The AA-line is the distance between Rt. & Lt. acupuncture point keug-cheun. The BB-line is the length of lung. The CC-line is the longest width of thorax. The DD-line is the vertical distance between the CC-line and the EE-line. The EE-line is the distance between the Rt. and Lt. anterior superior iliac spines. As the results macro-negative(strong liver-weak lung) has the longest CC-line and the short BB-line. Micro-negative(strong kidney-weak spleen) has the longest EE-line and the short CC-line. Micro-positive(strong spleen-weak kidney) has the longest DD-line and the short BB-line. And macro-positive(strong lung-weak kidney) has the longest BB-line and the short EE-line.

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The Effect of Binder Content for the Pore Properties of Fe Foam Fabricated by Slurry Coating Process (슬러리 코팅 공정으로 제조된 Fe 폼의 기공 특성에 미치는 바인더 함량의 영향)

  • Choi, Jin Ho;Yang, Sangsun;Kim, Yang-Do;Yun, Jung-Yeul
    • Journal of Powder Materials
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    • v.20 no.6
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    • pp.439-444
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    • 2013
  • Fe foam with above 90% porosity and 2 millimeter pore size was successfully fabricated by a slurry coating process. In this study, the binder contents were controlled to produce the Fe foam with different pore size, strut thickness and porosity. Firstly, the slurry was prepared by uniform mixing with Fe powders, distilled water and polyvinyl alcohol(PVA) as initial materials. After slurry coating on the polyurethane(PU) foam the sample was dried at $80^{\circ}C$. The PVA and PU foams were then removed by heating at $700^{\circ}C$ for 3 hours. The debinded samples were subsequently sintered at $1250^{\circ}C$ with holding time of 3 hours under hydrogen atmosphere. The three dimensional geometries of the obtained Fe foams with open cell structure were investigated using X-ray micro CT(computed tomography) as well as the pore morphology, size and phase.

Evaluation of Micro-defects and Air Tightness of Al Die-casting by Impregnation of Organic Solvent (유기용제 함침법을 통한 알루미늄 다이캐스팅의 미세결함 및 기밀성 평가)

  • Lee, Jin-Wook;Cho, Chang-hyun;Kim, Sung-Gye;Ko, Young-Gun;Kim, Dong-Ju
    • Journal of Korea Foundry Society
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    • v.42 no.4
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    • pp.218-225
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    • 2022
  • For hydrogen-vehicle applications (air pressure control valve housing, APCVH), an investigation was conducted to determine how micro-defects in a high- pressure die-casted Al alloy (industrial code: ALDC12) could be controlled by means of a post-treatment using an organic-based impregnation solution in order to improve the air- tightness of the die-casted Al sample. Two different impregnation solutions were proposed and its test results were compared to a imported product from Japan with respect to the processing variables used. A structural investigation of the components under study was conducted by means of computer tomography and 3D X-ray micro-CT. These observations revealed that the use of the impregnation treatment to seal micro-defects led to highly significant and beneficial changes which were attributed mainly to interconnections among inherent micro-pores. A leak test after impregnation revealed that the performance improvement rate of the die-casted Al sample was ~70% for INNO-01. Therefore, the developed impregnation solutions offer an effective strategy to control the micro-defects found in various vehicle parts via die-casting.

3D Histology Using the Synchrotron Radiation Propagation Phase Contrast Cryo-microCT (방사광 전파위상대조 동결미세단층촬영법을 활용한 3차원 조직학)

  • Kim, Ju-Heon;Han, Sung-Mi;Song, Hyun-Ouk;Seo, Youn-Kyung;Moon, Young-Suk;Kim, Hong-Tae
    • Anatomy & Biological Anthropology
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    • v.31 no.4
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    • pp.133-142
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    • 2018
  • 3D histology is a imaging system for the 3D structural information of cells or tissues. The synchrotron radiation propagation phase contrast micro-CT has been used in 3D imaging methods. However, the simple phase contrast micro-CT did not give sufficient micro-structural information when the specimen contains soft elements, as is the case with many biomedical tissue samples. The purpose of this study is to develop a new technique to enhance the phase contrast effect for soft tissue imaging. Experiments were performed at the imaging beam lines of Pohang Accelerator Laboratory (PAL). The biomedical tissue samples under frozen state was mounted on a computer-controlled precision stage and rotated in $0.18^{\circ}$ increments through $180^{\circ}$. An X-ray shadow of a specimen was converted into a visual image on the surface of a CdWO4 scintillator that was magnified using a microscopic objective lens(X5 or X20) before being captured with a digital CCD camera. 3-dimensional volume images of the specimen were obtained by applying a filtered back-projection algorithm to the projection images using a software package OCTOPUS. Surface reconstruction and volume segmentation and rendering were performed were performed using Amira software. In this study, We found that synchrotron phase contrast imaging of frozen tissue samples has higher contrast power for soft tissue than that of non-frozen samples. In conclusion, synchrotron radiation propagation phase contrast cryo-microCT imaging offers a promising tool for non-destructive high resolution 3D histology.

Micro-computed tomographic evaluation of a new system for root canal filling using calcium silicate-based root canal sealers

  • Tanomaru-Filho, Mario;Torres, Fernanda Ferrari Esteves;Pinto, Jader Camilo;Santos-Junior, Airton Oliveira;Tavares, Karina Ines Medina Carita;Guerreiro-Tanomaru, Juliane Maria
    • Restorative Dentistry and Endodontics
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    • v.45 no.3
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    • pp.34.1-34.7
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    • 2020
  • Objectives: This study evaluated by using micro-computed tomography (micro-CT) the filling ability and sealer apical extrusion promoted by a new Sealer Injection System (SIS; Angelus) with side openings needle, in comparison with the conventional injection system, associated with a new ready-to-use calcium silicate-based sealer (Bio-C Sealer). Materials and Methods: Acrylic resin models containing a main curved artificial canal and 3 simulated lateral canals in apical, middle and cervical thirds were used. The main root canals were prepared using a rotary system up to size 35.05. The canals were filled with Bio-C sealer by using a single cone technique and the conventional delivery system or SIS. Samples were scanned in micro-CT. The percentage of voids throughout the entire extension of the main root canal and in each third of the lateral canals, besides the apical extrusion of the sealer was calculated. Data were submitted to t-test (p < 0.05). Results: There was no difference between both systems in the main root canals filling. Although the volume percentage of voids was similar in the apical and middle thirds of lateral canals, SIS had the greatest filling ability of the cervical third lateral canal. Moreover, the conventional system showed the highest apical extrusion of the sealer. Conclusions: The conventional and SIS obturation systems had an appropriate filling ability of the main root canal. SIS had the best filling of the cervical third of the lateral canals, besides lower sealer apical extrusion, suggesting its clinical indication.