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http://dx.doi.org/10.5392/JKCA.2010.10.9.259

Microvascular Contrast Image in Portal Veins of Rat using Micro-CT  

Lee, Sang-Ho (익산병원 영상의학과)
Lim, Cheong-Hwan (한서대학교 방사선학과)
Jung, Hong-Rayng (한서대학교 방사선학과)
Han, Beom-Hee (서남대학교 방사선학과)
Mo, Eun-Hee (원광대학교병원 영상의학과)
Chai, Kyu-Yun (원광대학교 생명나노학부)
Publication Information
The Journal of the Korea Contents Association / v.10, no.9, 2010 , pp. 259-266 More about this Journal
Abstract
The study focuses on the value of Micro CT, a high resolution X-ray imaging device, by using it on rats to observe the overall portal vein image of the liver and the microvasculature of each lobes, visualize the 4 segmental lobes and acquire 3D image of the microvasculature through the reconstruction of sectional image data. Less of the damage to liver of the 5 mice, the device was able to separate the liver into 4 segmental lobes and displayed the 4 portal vein microvasculature in 2D. By using the 3D MIP technique, observation of the whole portal vein system microvasculature in 3D image was made possible along with each of the portal vein segment's branches until the 6th branch. Measured the size of 6branch, the average was measured at 1branch : $0.51mm{\pm}0.08$, 2 branch : $0.32mm{\pm}0.12$, 3 branch : $0.23mm{\pm}0.11$, 4 branch : $0.19mm{\pm}0.08$, 5 branch : $0.13mm{\pm}0.06$, 6 branch : $70.5{\mu}m{\pm}14.1$. The 3D image and the images of the microvasculatures in the result of study proved that the Micro-CT can be considered many useful device in obtaining high resolution images.
Keywords
Micro CT; Microvasculature; 3D Image;
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1 C. A. Zarse, J. A. McAteer, A. J. Sommer, S. C. Kim, E. K. Hatt, and J. E. Lingeman, "Nondestructive analysis of urinary calculi using micro computed tomography," BMC Urol, 13:4-15, 2004.
2 A. C. Langheinrich, R. M. Bohle, S. Greschus, N. Hackstein, G. Walker, and von Gerlach S, "Atherosclerotic l esions at micro CT: feasibility for analysis of coronary artery wall in autopsy specimens," Radiology, 231:675-681, 2004.   DOI   ScienceOn
3 Winter T III, Freeny P and H. Nghiem, "Hepatic arterial anatomy in transplantation candidates ; evaluation with three-dimensional CT arteriography," Radiology, 195:363-370, 1995.   DOI
4 E. Toyota, Y. Ogasawara, K. Fujimoto, T. Kajita, F. Higeto, and T. Asano, "Global heterogeneity of glomerular volume distribution in early diabetic nephropathy," Kidney Int, 66:855-61, 2004.   DOI   ScienceOn
5 엄태중, 강철, "바이오 포토닉스 영상기술," 물리학과첨단기술, pp.65-69, 2007(6).
6 S. Takahashi, T. Murakami, and M. Takamura, "Multi-detector row helical CT angiography of hepatic vessels : depiction with dual-arterial phase acquisition during single breath hold," Radiology, 222:81-88, 2002.   DOI   ScienceOn
7 J. W. Ludders, J. W. Wilson, and G. A. Ribble, "Microangiography and correlated histology: research technique for examining renal microcirculation," Am JVet Res,46:2536-8, 1985.
8 T. F. Massoud, "Gambhir imaging in living subjects; seeing fundamental processes in a new light," Genes Dev, 7:545-80, 2003.
9 M. D. Bentley, M. C. Ortiz, E. L. Ritman, and J. C. Romero, "The use of microcomputed tomography to study microvasculature in small rodents," Am J Physiol Regul Integr Comp Physiol, 282:F1267-79, 2002.   DOI   ScienceOn
10 P. Soyer, "Segmental anatomy of liver, Utility of a nomenclature accepted worldwide," AJR, 161:572-573, 1993.   DOI   ScienceOn
11 H. Bismuth, "Surgical anatomyh and anatomical surgery of the liver," World J Surg, 6:3-9, 1982   DOI
12 E. L. Ritman, "Micro-computed tomography-current statusand developments," Annu Rev Biomed Eng, Vol.6, pp.185-208, 2004.   DOI   ScienceOn
13 "Gray’s Anatomy," via Wikipedia, 1918.
14 T. Harada, "Liver and Gallbladder," Chapter 7 in Pathology of the Mouse.
15 E. L. Ritman, S. M. Jorgensen, P. E. Lund, P. J. Thomas, J. H. Dunsmuir, and J. C. Romero, "Synchrotron -based micro-CT of in-situ biological basic functional units and their integration," Proc Soc Photo Opt Instrum Eng : 3149:13-24, 1997.
16 Winter TCIII, Freeny PC and Nghiem HV, et al "Hepatic arterial anatomy in transplantation candidates: evaluation with three-dimensional CT arteriography," Radiology, 195:363-370, 1995.   DOI
17 S. Bourquin, Opt.Express, 3290, 2003.
18 J. F. Hainfeld, D. N. Slatkin, and H. M. Smilowitz, “The use of gold nanoparticles to enhance radiotherapy in mice,” Phys Med Biol, 49:N309- N315, 2004.   DOI   ScienceOn
19 G. F. Paciotti, L. Myer, and D. Weinreich, “Colloidal gold: a novel nanoparticle vector for tumor directed drug delivery,” Drug Deliv, Vol,11, pp.169-183, 2004.   DOI   ScienceOn
20 J. Chen, F. Saeki, and B. J. Wiley, “Gold nanocages bioconjugation and their potential use as optical imaging contrast agents,” Nano Lett, Vol.5, pp.473-477, 2005.   DOI   ScienceOn
21 M. J. Paulus, S. S. Gleason, S. J. Kennel, P. R. Hunsicker, and D. K. Johnson, "High resolution X-ray computed tomography: an emerging tool for small animal cancer research," Neoplasia, Vol.2, pp.62-70, 2000.   DOI
22 M. C. Daniel and D. Astruc, “Goldnanoparticles assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology,” Chem Rev, Vol.104, pp.293-346, 2004.   DOI   ScienceOn
23 R. L. sandberg, C. Song. P and W. Waehulak O, "A Raymondsono High numerical aperture tabletop soft x-ray diffratio microscopy with 10-nm redution," proc. Natl. Acad. Sci. U.S.A, 105-24, 2008.
24 C. T. Badea, B. Fubara, L. W. Hedlund, and G. A. Johnson, "4-D micro-CT of the mouse heart. Mol Imaging," Vol.4, pp.110-116, 2005.