• Korean Journal of Animal Reproduction
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• v.16 no.4
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• pp.289-299
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• 1993

The polypeptide patterns of cellular and follicular components were analysed by SDS-PAGE and two dimensional(2-D)electrophoresis combined with isoelectric focusing (IEF) to establish protein profiles in each of the components in porcine follicles. Oocyte-cumulus complexes were cultured in M16+FCS+Gn at 39 in an atmosphere of 5% CO$_2$, in air for 35 h. At the end of the culture, the zona-free oocyte, ZP alone and cumulus cells were prepared and analysed either on 10% SDS-PAGE for the protein profile at the first dimensional gel or 2-D protein pattern. The amounts of each samples were determined for the visualization with Coomasie brilliant blue (CBB) or silver staining, thus giving useful information for the identification of specific proteins in the components or appropriate amount of samples for proper visualization. Oocyte showed 25 and 114 kd major protein band. Other minor components were additionally visualized with CBB on the same gel after silver staining procedure. Cumulus cells also showed specific proteins which is not present in the oocytes. The number of cumulus cell was proper to give major bands with CBB and additional minor bands with silver staining. To establish the degree of contamination from the remnant of the corona radiata to the ZP, zonae were differently prepared or analysed by SDS-PAGE.The preparation of the ZP in this study did not showed any contamination judged by the protein profile of the components. Also follicular fluid showed its specific protein profile without any significant differences among the different sizes of follicles. The established protein profile of each follicular component should be helpful for the identification and elimination of contaminated components, i. e., antigen preparation or immunological studies. The results also suggest that the preparation of each components in the study was appropriate and can be used for a further sensitive biochemical analysis in mammalian oocytes and early embryos.

• Journal of The Korean Astronomical Society
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• v.37 no.1
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• pp.41-53
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• 2004

It has been a big mystery what drives filament eruptions and flares. We have studied in detail an X1.8 flare and its associated filament eruption that occurred in NOAA Active Region 9236 on November 24,2000. For this work we have analyzed high temporal (about 1 minute) and spatial (about 1 arcsec) resolution images taken by Michelson Doppler Imager (MDI) onboard the Solar and Heliospheric Observatory, Hoc centerline and blue wing ($-0.6{\AA}$) images from Big Bear Solar Observatory, and 1600 ${\AA}$ UV images by the Transition Region and Corona Explorer (TRACE). We have found that there were several transient brightenings seen in H$\alpha$ and, more noticeably in TRACE 1600 ${\AA}$ images around the preflare phase. A closer look at the UV brightenings in 1600 ${\AA}$ images reveals that they took place near one end of the erupting filament, and are a kind of jets supplying mass into the transient loops seen in 1600 ${\AA}$. These brightenings were also associated with canceling magnetic features (CMFs) as seen in the MDI magnetograms. The flux variations of these CMFs suggest that the flux cancellation may have been driven by the emergence of the new flux. For this event, we have estimated the ejection speeds of the filament ranging from 10 to 160 km $s^{-1}$ for the first twenty minutes. It is noted that the initiation of the filament eruption (as defined by the rise speed less than 20 km $s^{-1}$) coincided with the preflare activity characterized by UV brightenings and CMFs. The speed of the associated LASCO CME can be well extrapolated from the observed filament speed and its direction is consistent with those of the disturbed UV loops associated with the preflare activity. Supposing the H$\alpha$/UV transient brightenings and the canceling magnetic features are due to magnetic reconnect ion in the low atmosphere, our results may be strong observational evidence supporting that the initiation of the filament eruption and the preflare phase of the associated flare may be physically related to low-atmosphere magnetic reconnection.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.50 no.12
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• pp.599-606
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• 2001

In this paper, the ac breakdown characteristics of pure Ar, Kr and $N_2$ gas with gas pressure range of 58.8-137.3[kPa] under uniform and non-uniform fields were investigated, and the measured values were compared with those In Ar/$N_2$ and Kr/$N_2$ gas mixtures with pressure varying. Summarizing the experimental results, the breakdown voltages of Pure $N_2$gas, under uniform and non-uniform fields, were increased about 4.8 and 1.1 times than those of pure Ar gas, and about 4.4 and 1.2 times than those of pure Kr gas, and the ac breakdown voltage increased with the pressure increasing. The breakdown voltages of Ar/$N_2$ gas mixtures were decreased with decreasing the mixture ratio of Pure $N_2$ gas. In case of Ar(85%)/$N_2$ (15%) and Ar(70%)/$N_2$ (30%) gas mixtures comparing to the pure Ar gas, the breakdown voltages under uniform field were increased about 1.8 and 2.2 times, and under non-uniform field were increased about 1.1 and 1.3 times at the pressure of 101.3[kPa]. Also, in case of Kr(85%)/$N_2$ (15%) and Kr(70%)/$N_2$ (30%) gas mixtures comparing to the pure Kr gas, the breakdown voltages under uniform field were increased about 1.7 and 2.0 times, and under non-uniform field were increased about 1.0 and 1.2 times. Corona inception voltage of Kr(70%)/$N_2$(30%) gas mixtures under non-uniform fields were increased about 1.28 times than those of Ar(70%)/$N_2$ (30%) gas mixtures. In case of practical incandescent lamps, luminous and lifetime of Kr(70%)/$N_2$ (30%) gas mixtures were increased about 1.15 and 1.21 times than those of Ar(70%)/$N_2$ (30%) gas mixtures.

• The Journal of Bigdata
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• v.6 no.1
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• pp.179-196
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• 2021

With the spread of untact culture resulting from the Covid-19 pandemic, the size of the car camping market has expanded to minimize contact with others. As a result, SUVs have exceeded sales of sedans, and sales of recreational vehicles (RVs) have increased by 101% compared to the same period last year. Despite the explosive increase in demand for car camping, research on car camping has not matched this increase. Therefore, in this study, we intended to conduct a study focused on car camping users. According to a survey of Naver's famous car camping cafe, it was difficult to find articles, maps, and websites with car camping places. Analysis of car camping websites showed that most only post information about the camping itself, so details of car camping places were not available. Furthermore, according to a survey derived from related prior studies and literature surveys, most users urged solutions to the problem of unauthorized garbage dumping in the car camping locations. In addition, car camping users wanted to receive information on amenities near the car camping places. Therefore, we aimed to establish a car camping website that provides basic information on car camping places and nearby convenience facilities. Moreover, to solve the problem of garbage dumping, we provided a category wherein users can post pictures of clean camping campaigns. We also developed a business model utilizing the certification process of clean camping. The business model is designed with a structure wherein car camping users are rewarded through the clean camping certification process. Compensation for clean camping certification was proposed to be provided through partnerships with domestic automakers, Korea Tourism Organization, and Small Business Market Promotion Agency.

• Clinical and Experimental Reproductive Medicine
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• v.26 no.1
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• pp.75-81
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• 1999

The beneficial effect of glucose and phosphate ions in culture medium on the development of human embryos in vitro has not been fully elucidated. The purpose of this study was to evaluate the influence of fertilization and culture of embryos in glucose/phosphate-free m-TALP medium on pregnancy rates in IVF-ET program. The patients in 244 IVF-ET cycles received GnRH agonist + HMG regimens. A does of 10,000 IU HCG was administered when two or more dominent follicles reached 18mm in diameter. Thirty-six hours after HCG, oocytes were recovered transvaginally using ultrasound guidance. Aspirated oocytes were matured for 4 to 6 h in TCM-199 supplemented with 10% follicular fluid (FF). Insemination was carried out with 50,000 motile spermatozoa in TCM-199 + 10% FF or m-TALP + 5% FF + 5% fetal cord serum (FCS) according to experimental design. After 6 h, oocytes were washed 3 to 4 times and cultured in each fresh medium. After 20 h, oocytes were freed from cumulus/corona cells and examined for the presence of pronuclei. Fertilized oocytes were transferred into each co-culture drops and cultured for further incubation. On day 3, embryo transfer was performed with grade 1 and 2 embryos. Monolayers for co-culture of embryos were prepared by plating $1{\times}10^5$ cumulus cells/ml in 10ul drop of TCM-199 + 10% FF or m-TALP + 5% FF + 5% FCS media 24 h prior to the onset of co-culture. Development to 4 to 16 cell stage was observed at 70x magnification following two days of incubation. Pregnancy was confirmed by detecting increasing serum ${\beta}$-hCG concentrations for 11 days following embryo transfer. Data were analyzed by ${\chi}^2$-test. Oocytes from 244 IVF-ET cycles were randomized. The number of cycles and mean age of patients were 97 and 147, 31.3 yrs and 31.2 yrs for TCM-199 (control) and m-TALP groups, respectively. The mean number of retrieved oocytes/cycle, fertilization rates, number of embryos transferred/ET and pregnancy rates were 11.1 and 10.3, 65.1% and 67.3%, 4.1 and 4.7, 28.9% and 43.8% for TCM-199 and m-TALP groups, respectively. Differences in the pregnancy rates were found between control and m-TALP groups (p<0.05). The pregnancy rate of patients divided according to maternal age groups of ${\leq}30$, 31-35, $36{\leq}$ were 44.4% and 49.0%, 26.1% and 41.3%, 29.2% and 41.2% for control and m-TALP groups, respectively. These data indicate that culture of human embryos in glucose/phosphate-free m-TALP medium improves pregnancy rates.

• Journal of Digital Convergence
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• v.19 no.6
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• pp.133-141
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• 2021

Governments of each country are actively implementing fiscal expansion policies to recover the real economy after Corona 19. In Korea, the stock market and housing market are greatly affected as liquidity in the market increases due to the implementation of disaster subsidies and welfare policies. The purpose of this study is to analyze the relationship between stock market and housing market trends and liquidity. Data were collected by the Bank of Korea and Kookmin Bank. The analysis period is from January 2000 to December 2020, and monthly data are used. For empirical analysis, the rate of change from the same month of the previous year was calculated for each variable, and numerical analysis, index analysis, and model analysis were performed. As a result of the analysis, it was found that the stock index showed a positive(+) relationship with the house price, while a negative(-) relationship with M2. Previous studies have suggested that, in general, an increase in liquidity affects the stock market and the housing market, and inflation also rises. In this study, it was found that the stock market and the housing market had an effect on each other. However, it was investigated that liquidity showed an inverse relationship with the stock market and had no relationship with the housing market. Through this, this study estimated that there is a time difference in the relationship between liquidity and the stock market & housing market.

• Journal of the Korean Society of Radiology
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• v.15 no.4
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• pp.519-524
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• 2021

As the COVID-19 situation continued to spread to the local community along with the spread due to influx, each university had to conduct all online classes and partially online classes. The purpose of this study was to investigate the satisfaction of learners with the content and lecture contents by paying attention to online non-face-to-face classes according to the change of the class environment in the Corona 19 situation. Satisfaction survey on online non-face-to-face class major subjects was analyzed using questionnaires from June 1 to June 11, 2021, targeting 2nd and 3rd year students in the Department of Radiology at S University in Daegu. As a result of the study, satisfaction with content and class content was found to be an average of 3.78 ± 0.75 points, and learning satisfaction was found to be an average of 3.00 ± 1.14 points. In addition, when taking online non-face-to-face classes, the correlation between students' class attitude and content and class content satisfaction was the highest (r=0.555, p<0.01), and it was found that there was also a correlation between content and class content satisfaction and learning satisfaction. (r=0.331, p<0.01). I think that satisfaction with non-face-to-face online classes can be improved if the quality of content is improved during non-face-to-face online major classes as well as more active interactions between students and professors.

• The Journal of the Convergence on Culture Technology
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• v.8 no.2
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• pp.281-289
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• 2022

Our society is rapidly changing with the core technology of the 4th industrial revolution, the emergence of a generation with new characteristics, and the untact era following the With Corona policy. Although the transition to untact is accelerating in the field of performing arts, in the case of opera, face-to-face performances are mainly conducted by experts and enthusiasts through on-site performances. Therefore, it is necessary to consider the uncertainty of creating opportunities through the influx of new customers such as the MZ generation and the departure of existing experts and enthusiasts. In this study, in order to examine these existing problems, we conducted literature review and case analysis, compared the opera face-to-face/non-face-to-face performance characteristics, derived an activation plan, and conducted expert interviews to secure the coherence and validity of the plan. In conclusion, we thought that it was difficult to improve the sound and sound quality, impairing the sense of presence and emotion due to many shortcomings when operating non-face-to-face as a music genre with the characteristics of opera. Therefore, we established the direction of activating the opera mainly face-to-face, but making good use of the advantages of non-face-to-face, which is not limited by region and time, and promoting the direction of activating face-to-face and non-face-to-face performances complementary to each other through the concept of cultural enjoyment.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.511-523
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• 2022

This study was conducted using non-face-to-face care technology for the elderly with mild dementia and the physically weak living in the community, as various methods of care for the elderly have been raised due to the prolonged COVID-19. The purpose of this study is a similar experimental study before and after the inequality control group to compare cognitive function, daily living activities, and the degree of depression by applying an AI robot integrated management program using. The data was collected from June 4 to September 17, 2021, and the survey results of 17 people in the experimental group and 18 in the control group were analyzed using the SPSS 25.0 program. As a result of the study, the experimental group was significant in language function, activities of daily living, and depression. In particular, the results showed a decrease in moderate to severe depression and mild depression. Cognitive function was significant with long-term care grade and daily living activity with family living together. Therefore, if such non-face-to-face care technology is introduced to the elderly care field in the 'With Corona era', it is thought that it will contribute to cognitive function training and depression reduction of the elderly.

• Journal of the Korean Applied Science and Technology
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• v.40 no.1
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• pp.113-122
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• 2023

This study attempted to investigate the effects of experience of non-contact lectures on learning flow against college students majoring in cosmetology and provide basic data to beauty education industry in the with-COVID-19 era. For this, a self-administered questionnaire survey was performed against 300 college students majoring in cosmetology from June 7 to 21, 2022. A total of 286 copies were collected and used for final analysis. The collected data were analyzed by frequency analysis, factor analysis, exploratory factor analysis, descriptive statistics, correlation analysis and multiple regression analysis, using SPSS 21.0, and the results found the followings: According to analysis of non-contact lecture experience factors, two course satisfaction factors were obtained. In learning flow, learning pleasure and learning flow were found. Specifically, class activities had a positive influence on 'learning pleasure (𝛽=.279, p<.007)' and 'learning flow (𝛽=.221, p<.031)' with statistical significance (p<.05). In addition, course satisfaction revealed a positive effect on 'learning flow (𝛽=.223, p<.041)' with statistical significance (p<.05). The above results confirm that experience of non-contact lectures affects learning flow. Therefore, it is anticipated that there would be more efforts to seek an efficient non-contact learning plan in this new era.