• Title/Summary/Keyword: Wild yeasts

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한국산 야생효모에 관한 연구 3

  • 박명삼
    • Korean Journal of Microbiology
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    • v.10 no.2
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    • pp.51-68
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    • 1972
  • The aim of the sutdy is to collect a variety of wild yeasts from different regions in Korea and in different seasons and to account for the natural patterns of regional and seasonal variation that they display. From the specimens collected in this study, more useful strains are expected to be discovered, which can be cultivated and utilized fro industrial development. The study attempts to determine the degree to which utilizable yeasts can be applied in brewing, confectionary, baking, the manufacture of medicine, and as feed yeast. Such findings would contribute not only to the development of academic research, but would also be important in obtaining raw material that can be applied in our daily lives and in industrial development in response to the demands of the times.

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Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

  • Lee, Sang Eun;Oh, Hye Jin;Kim, Myung Kyum
    • Journal of Species Research
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    • v.10 no.4
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    • pp.344-349
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    • 2021
  • The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

Production of an Anti-dementia Butyrylcholinesterase Inhibitor from Non-pathogenic Wild Yeast, Saccharomyces cerevisiae WJSL 0113 (비병원성 야생효모 Saccharomyces cerevisiae WJSL 0113으로부터 항치매성 Butyrylcholinesterase 저해물질의 생산)

  • Han, Sang-Min;Park, Seon-Jeong;Jang, Ji-Eun;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.49 no.2
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    • pp.243-248
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    • 2021
  • In this study, screening of potent non-pathogenic wild yeast with high anti-dementia butyrylcholinesterase (BChE) inhibitory activity and production condition of a BChE inhibitor were described. Among 36 non-pathogenic wild yeasts, Saccharomyces cerevisiae WJSL 0113 showed the highest BChE inhibitory activity of 85.2%. The specific BChE inhibitor was maximally produced when S. cerevisiae WJSL 0113 was cultured at 30℃ for 48 h in a yeast extract-peptone-dextrose medium.

Isolation of Yeasts from Wild Flowers in Gyonggi-do Province and Jeju Island in Korea and the Production of Anti-gout Xanthine Oxidase Inhibitor (경기도와 제주도 야생화들로부터 효모의 분리, 동정 및 항통풍성 Xanthine oxidase 저해물질의 생산)

  • Hyun, Se Hee;Mun, Hye Yeon;Lee, Hyang Burm;Kim, Ha-Kun;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
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    • v.41 no.4
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    • pp.383-390
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    • 2013
  • Several yeasts were isolated from flowers found in Gyonggi-do Province and Jeju island in Korea. They were then identified by a comparison of their PCR-amplified D1/D2 regions of 26S rDNA, internal transcribed spacer 1 and 2 inclusive of 5.8S rDNA, using the BLAST database. A total of fifty four yeast strains were isolated from wild flowers in Gyonggi-do and the genus Pseudozyma was noted as being dominant. A total of thirty two strains were isolated from Songaksan and Seongsan-ilchulbong in Jeju island and Sporobolomyces ruberrimus was seen to be dominant. The anti-gout xanthine oxidase inhibitory activities of the culture broths and cell-free extracts from eighty six yeast strains were then determined. The cell-free extracts of Pseudozyma hubeiensis 228-S-1 exhibited the highest xanthine oxidase inhibitory activity of 19.6%. The XOD inhibitor was also maximally produced when Pseudozyma hubeiensis 228-S-1 was cultured at $30^{\circ}C$ for 36h in YEPD medium.

Antioxidant Activity and Inhibitory Activities of Xanthine Oxidase and Tyrosinase of Yeasts from Wild Flowers in Korea (우리나라 야생화에서 분리한 효모의 항산화 활성과 Xanthine Oxidase 및 Tyrosinase 저해활성)

  • Han, Sang-Min;Hyun, Se-Hee;Kim, Na-Mi;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.43 no.2
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    • pp.99-103
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    • 2015
  • We prepared supernatants and cell-free extracts of yeasts isolated from wild flowers in Korea, and their antioxidant activity and inhibitory activities of xanthine oxidase and tyrosinase were investigated. Among them, cell-free extracts of Kuraishia capsulata UL40-2 and Sporobolomyces ruberrimus 121-Z-3 showed significantly high xanthine oxidase inhibitory activity of 46.4% and 48.3%, respectively. Starmerella bombicola 80-J-1 also showed tyrosinase inhibitory activity of 36.2% in the cell-free extract. Other antioxidant activities and tyrosinase inhibitory activities were not detected or were below 20%. Maximal production of the xanthine oxidase inhibitors were observed when Kuraishia capsulate UL40-2 and Sporobolomyces ruberrimus 121-Z-3 were cultured in the yeast extract-peptone-dextrose media at $30^{\circ}C$ for 24 hour, respectively.

Physiological Functionalities and Enzyme Activities of Non-pathogenic Pigmented Wild Yeasts (비병원성 야생 색소 효모들의 생리활성과 효소활성)

  • Jeong-Su Moon;Seung-A Shin;Seung-Eun Baek;Jong-Soo Lee
    • The Korean Journal of Mycology
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    • v.51 no.3
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    • pp.205-217
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    • 2023
  • This study aimed to produce novel bioactive compounds from non-pathogenic pigmented wild yeasts. Culture supernatants and cell-free extracts of non-pathogenic pigmented yeast strains were prepared, and their physiological functionalities and enzyme activities were measured. Cell-free extracts from Rhodosporidium paludigenum HHGG35-1 and culture supernatants from Rhodosporidium diobovatum NMD18-1 demonstrated very high antioxidant activity (76.6%) and anti-gout xanthin oxidase inhibitory activity (86.2%), respectively. Maximal production of the antioxidants (76.9%) was obtained when Rh. Paludigenum HHGG35-1 was cultured in a yeasts extract-peptone-dextrose (YPD) medium (pH 6.5) at 30℃ for 24 h. The xanthin oxidase inhibitor was also maximally produced (91.6%) when Rh. Diobovatum NMD18-1 was cultured at 30℃ for 96h in a YPD medium (pH 6.5). Rh. Paludigenum HHGG35-1 was oval in shape and formed ascospre. The Rh.diobovatum NMD18-1 specimen displayed dimensions of 1.6 × 1.6 ㎛ and produced ascospores; however, it did not form pseudomycelium. Both of Rh. Paludigenum HHGG35-1 and Rh. Diobovatum NMD18-1 grew well in a 40%-glucose-containing YPD medium and 10%-NaCl-containing YPD medium.

Screening of Anti-inflammatory Compound-producing Wild Yeasts and Their Microbiological Characteristics (항염증 물질 생산 능력이 우수한 야생효모의 선별 및 이들의 균학적 특성)

  • Bae, Sang-Min;Han, Sang-Min;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.45 no.3
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    • pp.212-223
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    • 2017
  • To screen for potent anti-inflammatory compound-producing yeasts, we evaluated nitric oxide production inhibitory activities in RAW 264.7 macrophage cells using cell-free extracts from 182 non-pathogenic yeasts. Rhodotorula graminis YJ36-1 and Meyerozyma guilliermondii YJ34-2 showed high inhibitory activities of 57.4% and 47.0%, respectively. The microbiological characteristics of these yeasts were investigated. Rhodotorula graminis YJ36-1 formed ascospores and pseudomycelium. This species grew well at $25^{\circ}C$ in yeast extract-peptone-dextrose (YPD) medium, vitamin-free medium, and 5% NaCl-containing YPD medium. Meyerozyma guilliermondii YJ34-2 was an asporogenous yeast and did not form pseudomycelium. This strain also grew well at $30^{\circ}C$ in YPD medium, vitamin-free medium, and 5% NaCl-containing YPD medium.

Characterization of Unrecorded Yeasts Isolated from Leaves of Trees of Oknyeobong Peak and Yeonjasan Mountain in Daejeon, Korea (대전광역시 옥녀봉과 연자산 나뭇잎으로부터 야생효모의 분리 및 국내 미기록 효모의 특성)

  • Han, Sang-Min;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.45 no.1
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    • pp.23-30
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    • 2017
  • Twenty-two yeast strains of 15 species were isolated from the leaves of 20 trees on Oknyeobong Peak, and 24 yeast strains of 12 species were isolated from the leaves of 20 trees on Yeonjasan Mountain in Daejeon city, Korea. Cryptococcus bestiolae (5 strains) and Aureobasidium pullulans (8 strains) were the predominant isolates from Oknyeobong Peak and Yeonjasan Mountain, respectively. Of a total of 46 yeast strains, Cryptococcus kuetzingii JSL508, Cryptococcus vishniacii JSL509, and Dioszegia takashimae JSL510 from Okyeobong Peak, and Plowrightia periclymeni JSL514, Erythrobasidium hasegawanium JSL0193, and Rhodotorula nothofagi JSL0196 from Yeonjasan Mountain were determined to be yeast strains that were yet unrecorded in Korea. Morphological and cultural characteristics of these unrecorded yeasts were investigated. Erythrobasidium hasegawanium JSL0193 and Rhodotorula nothofagi JSL0196 did not form ascospores and pseudomycelia. All the strains, except Dioszegia takashimae JSL510, were halotolerant or halophilic, and Cryptococcus kuetzingii JSL508 and Dioszegia takashimae JSL510 were thermophilic, growing at $37^{\circ}C$.

First Record of Four Yeast Strains Isolated from Freshwater in Korea (국내 담수환경에서 분리된 국내 미기록 효모 4종 보고)

  • Yoosun Oh;Wonsu Cheon;Hye Yeon Mun;Jaeduk Goh
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.349-359
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    • 2023
  • Freshwater ecosystems are significant habitats of fungi including yeasts. This study aimed to isolate and characterize wild yeasts from freshwater environments in Korea. The yeast isolates were identified by using the D1/D2 domains of the 26S rDNA regions. We identified four strains, Candida viswanathii (NNIBRFG39781), Curvibasidium cygneicollum (NNIBRFG49003), Oberwinklerozyma silvestris (NNIBRFG39803) and Vishniacozyma foliicola (NNIBRFG6120). These yeasts had not previously been recorded in Korea. We investigated the morphological and cultural characteristics of these yeasts. All of them grew on YPD, PD and YM media. Candida viswanathii (NNIBRFG39781), O. silvestris (NNIBRFG39803) and V. foliicola (NNIBRFG6120) grew in YPD medium containing glucose and in pH range of 4-8. Curvibasidium cygneicollum (NNIBRFG49003) grew well low temperature compared to others and slowly.

Screenig and Indentification of Wild Strains for the Production of High Concentration of Alcohol from Jerusalem artichoke Tubers (돼지감자를 이용한 고농도 알코올발효 균주의 탐색)

  • Hong, Yeun;Choi, Eon-Ho
    • Microbiology and Biotechnology Letters
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    • v.22 no.6
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    • pp.707-712
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    • 1994
  • Yeast screening for effective production of alcohol from Jerusalem artichoke tubers as an alternative energy source was performed. Inulin assimilative strains with high alcohol tolera- nce were isolated from wild sources and cultured in the liquid media of Jerusalem artichoke powder varying its concentraion from 15 to 30%. As a result, four strains of 2,445 isolates showing the inulin assimilation were selected as alcohol fermentative and alcohol tolerant yeasts. These strains were assignated to be Kluyveromyces marxianus F043 and Kluyveromyces sp. F173, E040, and F334, respectively, by their cultural and physiological characteristics. The F043 strain produced ethanol of 98.1 g/l in the 25% Jerusalem artichoke medium for 3 days.

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