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http://dx.doi.org/10.4014/kjmb.1309.09002

Isolation of Yeasts from Wild Flowers in Gyonggi-do Province and Jeju Island in Korea and the Production of Anti-gout Xanthine Oxidase Inhibitor  

Hyun, Se Hee (Department of Biomedicinal Science and Bioechnology, Paichai University)
Mun, Hye Yeon (Divison of Applied Bioscience & Biotechnology, Chonnam National University)
Lee, Hyang Burm (Divison of Applied Bioscience & Biotechnology, Chonnam National University)
Kim, Ha-Kun (Department of Biomedicinal Science and Bioechnology, Paichai University)
Lee, Jong-Soo (Department of Biomedicinal Science and Bioechnology, Paichai University)
Publication Information
Microbiology and Biotechnology Letters / v.41, no.4, 2013 , pp. 383-390 More about this Journal
Abstract
Several yeasts were isolated from flowers found in Gyonggi-do Province and Jeju island in Korea. They were then identified by a comparison of their PCR-amplified D1/D2 regions of 26S rDNA, internal transcribed spacer 1 and 2 inclusive of 5.8S rDNA, using the BLAST database. A total of fifty four yeast strains were isolated from wild flowers in Gyonggi-do and the genus Pseudozyma was noted as being dominant. A total of thirty two strains were isolated from Songaksan and Seongsan-ilchulbong in Jeju island and Sporobolomyces ruberrimus was seen to be dominant. The anti-gout xanthine oxidase inhibitory activities of the culture broths and cell-free extracts from eighty six yeast strains were then determined. The cell-free extracts of Pseudozyma hubeiensis 228-S-1 exhibited the highest xanthine oxidase inhibitory activity of 19.6%. The XOD inhibitor was also maximally produced when Pseudozyma hubeiensis 228-S-1 was cultured at $30^{\circ}C$ for 36h in YEPD medium.
Keywords
26S rDNA; ITS 1/5.8S rDNA/ITS2; wild flowers; yeasts; anti-gout xanthine oxidase inhibitor;
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Times Cited By KSCI : 10  (Citation Analysis)
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