The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Production of an Anti-dementia Butyrylcholinesterase Inhibitor from Non-pathogenic Wild Yeast, Saccharomyces cerevisiae WJSL 0113

비병원성 야생효모 Saccharomyces cerevisiae WJSL 0113으로부터 항치매성 Butyrylcholinesterase 저해물질의 생산

  • Han, Sang-Min (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Park, Seon-Jeong (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Jang, Ji-Eun (Faculty of Biomedicine and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Faculty of Biomedicine and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오의약학부) ;
  • 박선정 (배재대학교 바이오의약학부) ;
  • 장지은 (배재대학교 바이오의약학부) ;
  • 이종수 (배재대학교 바이오의약학부)
  • Received : 2021.02.10
  • Accepted : 2021.04.20
  • Published : 2021.06.30
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Abstract

In this study, screening of potent non-pathogenic wild yeast with high anti-dementia butyrylcholinesterase (BChE) inhibitory activity and production condition of a BChE inhibitor were described. Among 36 non-pathogenic wild yeasts, Saccharomyces cerevisiae WJSL 0113 showed the highest BChE inhibitory activity of 85.2%. The specific BChE inhibitor was maximally produced when S. cerevisiae WJSL 0113 was cultured at 30℃ for 48 h in a yeast extract-peptone-dextrose medium.

본 연구에서는 진균류 유래의 새로운 항치매성 BChE저해물질을 생산할 목적으로 대전광역시 하천에서 분리하여 선별한 비병원성 야생효모들의 무세포추출물들을 제조하여 이들의 BChE 저해활성 측정하였다. 비병원성 야생효모들 중 S. cerevisiae WJSL0113의 무세포추출물이 85.2%의 BChE 저해활성을 보여 최종 선발하였다. 선발 균주의 BChE 저해활성은 시판 주류용 S. cerevisiae과 식용 버섯들의 추출물보다 높은 저해활성을 보였고 선발 균주인 S. cerevisiae WJSL0113을 30℃에서 48시간 배양하였을 때 가장 높은 항치매성 BChE 저해활성을 보였다.

Keywords

References

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