• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.1
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• pp.52-58
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• 1991

Physico-chemical properties including the composition of organic acids and fatty acids in native bee-honey and foreign bee-honey harvested in Kangwon area were studied with the emphasis on the honey which was collected form wild flowers nd mixed flowers sources for honey nectars. the major organic acids were considered as acetic acid formic acid and valeric acid in volatile acids and gluconic acid maleic acid malic acid quinic acid and citric acid in non-volatile fraction in both of native bee-honey and foreigr bee-honey. Some naturally occuring fatty acids({{{{ { C}_14{ } }}}}-{{{{ {C }_{20 } }}}}) were observed with the principal fatty acids of myristic aicd linolenic acid and palmitic acid and palmitic acid in the various honey, The characteristics of moisture content pH viscosity free acidity lactone and total acidity were also analyzed and discussed.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.4 no.3
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• pp.84-95
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• 2001

This study was conducted to suggest the ecological restoration methods for the decomposed granite roadside cutslopes by Natural Topsoil Restoration Methods(NTRM). Four woody species, 3 shrub species, 4 native grasses, 8 wild flowers and 2 cool-season grasses were used. The experiment was conducted with the purpose of developing ecological restoration techniques by achieving following objectives for restoration by using native woody plants, shrubs, native plants and wild flowers; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Restoring naturalness to human-altered landscapes are important to protecting the worlds biodiversity. In this study, to restore or create an ecological community use as full a complement of native species as possible. Ecological restoration means returning a specific sites to its predisturbance condition. 2. Some species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. In this study, cool season grasses was used small amount than native plants and woody species, because and Indigofera pseudo-tintoria grows well. Tall fescue and perennial ryegrass shows quick germination, but they could not inhibit the other species growing relatively slow. Pinus thunbergii used at Gonam near the ocean was suppressed by the other plants, so it can be concluded that using container seedlings is effective than seeding. 4. Ailanthus altissima, Albizzia julibrissin, Rhus chinensis Lespedeza cyrtobotrya, and Indigofera pseudo-tinctoria were germinated. In wild flowers, Coreopsis lanceolata, Dianthus sinensis, Coreopsis tinctoria, and Lotus corniculatus var. japonicus grew well and show enough plant height which will not suppressed by shrub and woody plants. After one year, native grasses such as Arundinella hirta, Miscanthus sinensis var. purpurascens and Imperata cylindrica var. koenigii cover the slopes well. 5. 25 herbaceous plants such as Trifolium repens, Arthraxon hispidus, Barbarea orthocerae, Potentilla fragarioides, Lepidium apetalum, Chenopodium album var. centrorubrum, Glycine soja, Rubus crataegifolius and 4 woody species such as Lespedeza maximowiczii, Robinia pseudoacacia, Symplocos Chinensis, Quercus acutissma were invaded. 6. Ecological restoration works by Natural Topsoil Restoration Methods for revegetation of the decomposed granite roadside cutslopes were successfully accomplished. It prevents surface water erosion and shows diverse vegetation structure. It will be successful to restore decomposed granite cutslopes.

• FLOWER RESEARCH JOURNAL
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• v.19 no.4
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• pp.212-218
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• 2011

Edible flowers are harvested at the full bloom stage and distributed without floral stems, which makes the flower quality deteriorated quickly. Thereby, this study was carried out for selection of effective storage film at room temperature during the distribution of edible flowers. Edible flowers, snapdragon (Antirrhinum majus), pot-marigold (Calendula officinalis), sweet violet (Viola odorata), clove pink (Dianthus caryophyllus), wild pansy (Viola tricolor), nasturtium (Tropaeolum majus) were used to select the effective storage film at room temperature during the distribution, polyethylene (PE) film (0.03 mm), polypropylene (PP) film (0.03 mm). PP (LF10) film packing was very effective for longer shelf life for sweet violet, clove pink, and wild pansy. But, there were little different for pot-marigold and nasturtium. Wilting at sweet violet, clove pink, and wild pansy, and petal-soft rot and color break at nasturtium were appeared. Browning and rot were appeared to snapdragon.

• Communications for Statistical Applications and Methods
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• v.8 no.3
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• pp.739-751
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• 2001

In this paper, a sample design is studied for 2000 forestry management survey of five types forestry , tree felling, gathering of pine mushroom, growing of nut trees, growing of wild flowers, and lumbering industry. We introduce population stratification and a modified stratified cut-off sampling which deal with determination of sample size, sample allocation, and estimation of total and variance of estimator. Substitution of sample units and imputation of nonresponse units are discussed for reducing the nonsampling errors.

• The Korea Journal of Herbology
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• v.28 no.5
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• pp.13-19
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• 2013

Objectives : The purpose of this study is correct the scientific name of Aquilariae Lignum in Korean Herbal Pharmacoepia. Methods : The production areas of Aquilariae Lignum and its trading status with China in Chinese history, Sanscrit-Chinese Translation Sutra, Naming year and the discovered district in main Aquilaria spp., Several nation's Pharmacoepia, The Plant List(TPL), Convention on International Trade in Endangered Species of Wild fauna and flora(CITES) and The International Union for Conservation of Nature(IUCN) were cross-checked. Results : The records in the Jiaozhouyiwuzhi written in the early 2nd century said that Aquilariae Lignum was produced in Vietnam. NanfangCaomuZhuang written in 304 said that Agarwood in Vietnam had white flowers. Vietnam had led production and trading of Aquilariae Lignum until Qing Dynasty. Aquilariae Lignum from Malaysia and Indonesia was not traded with China. In Sanscrit-Chinese Translation Sutra, India Aquilariae Lignum was translated as Vietnam Aquilariae Lignum. Aquilaria malaccensis was discovered from Malay-Peninsular in 1783, and has green or dirty yellow flowers. A. agallocha from North-Eastern India in 1814, white flowers. A. crasssna from Vietnam in 1914, white flowers. A. crassna is different from A. malaccensis in several ways, such as flower, fruit, seed and disribution. In several Nation's Pharmacoepia, A. crassna was a synonym of A. agallocha. But in TPL, CITES and IUCN, A. malaccensis was an accepted name, and A. agallocha was a synonym of A. malaccensis. Conclusions : These results show that the original species of Aquilariae Lignum in Korea Herbal Pharmacoepia should be reversed from A. agallocha to A. crassna Pierre ex Lacomte.

• Journal of Plant Biotechnology
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• v.47 no.2
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• pp.172-178
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• 2020

Fruit-specific promoters play an important role in the improvement of traits, such as fruit quality through genetic engineering. In tomato, the development of fruit-specific promoters was previously reported, but less attention has been paid to the promoters involved in the fruit development stage. In this study, we characterized the gene expression patterns of tomato alcohol dehydrogenase 2 (SlAdh2) in various tissues of wild-type tomato (cv. Ailsa Craig). Our findings revealed that SlAdh2 expression levels were higher in the developing fruit than in the leaves, stems, and flowers. The ProSlAdh2 region, which is expressed at different stages of fruit development, was isolated from tomato genomic DNA. Following this, it was fused with a β-glucuronidase reporter gene (GUS) and introduced into wild-type tomato using Agrobacterium-mediated transformation to evaluate promoter activity in the various tissues of transgenic tomato. The ProSlAdh2:GUS promoter exhibited strong activity in the fruit and weak activity in the stems, but displayed undetectable activity in the leaves and flowers. Interestingly, the promoter was active from the appearance of the green fruit (1 cm in size) to the well-ripened stage in transgenic tomatoes, indicating its suitability for transgene expression during fruit development and ripening. Thus, our findings suggest that ProSlAdh2 may serve as a potential fruit-specific promoter for genetic-based improvement of tomato fruit quality.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.271-286
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• 2017

GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors containing two conserved QLQ and WRC domains and play critical roles in regulating the growth and development of lateral organs, such as cotyledons, leaves, and flowers. To explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), the researchers isolated seven BrGRFs (BrGRF3-1, 3-2, 5, 7, 8-1, 8-2, and 9) and constructed BrGRF-overexpressing Arabidopsis thaliana plants (BrGRF-OX). BrGRF-OX plants developed larger cotyledons, leaves, and flowers as well as longer roots than the wild type. The increase in size of these organs were due to increases in cell number, but not due to cell size. BrGRF-OX plants also had larger siliques and seeds. Furthermore, BrGRF-OX seeds produced more oil than the wild type. RT-PCR analysis revealed that BrGRFs regulated expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, the data indicates that BrGRFs act as positive regulators of plant growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed oil production.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.71-80
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• 2019

We have been experimenting for industrialize four kinds of wildflowers(Smilax china, Saururus chinensis, Houttuynia cordata and Zizania caduciflora) which are used for Korean medicinal plants for a long time as Korean resources plants. Smilax china, Saururus chinensis, Houttuynia cordata was used for roots and Zizania caduciflora used for leaves. Extraction was carried out in 50% ethyl alcohol, which was concentrated by freezing the material and then injected at a low concentration of 100ppm. The results of the study on the antibacterial potential activity and antifungal potential activity using the four wildflowers strains were as follows. 1. The results of the sterilization and antibacterial potential activity and antifungal potential activity were as follows. Houttuynia cordata showed good inhibitory effect for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The inhibitory power of Zizania caduciflora was proved to some extent. However, other cultivars showed little antibacterial and antifungal activity. 2. The antifungal potential activity against Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Cryptococcus neoformans was small but some inhibition against Candida albicans was confirmed. There was no inhibition of other fungi potential at all.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.4
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• pp.70-82
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• 2007

Aiming at nature's early restoring needs to select plant species harmonizing with surrounding environment that fits to restoring goal of ultimately aiming at woody plants dominant vegetation that protect and stabilize surface parts of bared slopes. And it is important to make it assimilated with surrounding natural vegetation by differentiating planning of seed mixtures. Natural Ecological Restoring Construction Methods (JSB Method) was developed to increase the effect of landscape change according to seasons not to simple hydro-seeding by reducing rate of foreign grasses while raising rate of wild flower using. It was considered that using wild flowers that bloom on each season solved the problems of slope landscapes that looks artificial and uninteresting. After researching environmental condition of the slopes, JSB Method has shown that, as of wet-type method, soil is relatively soft and the difference of thickness of plant base soil media affects on the difference of soil hardness. In case of soil hardness of 8.8~17mm, there's dangerousness of slope's collapsing, but it has shown that growth of plant was favorable. For the acidity of soil, most of them are in the type of neutralized soil of more than pH 6. And after analyzing the degree of woody plants dominance, it had shown that lower part was occupied with wild flowers and other herbaceous species like China pin, golden phesant mum, pitcher plant, and middle and upper part was occupied with woody plants like silk-tree, sumac forming multi-layer structure. It can be concluded that the restoration objects of the woody plant vegetation on the roadside slopes can be accomplished successfully by Natural Ecological Restoring Construction Method (JSB method).

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.475-484
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• 2020

Various indigenous yeasts were isolated and obtained from flowers in the Republic of Korea, and their distribution and species diversity were studied. Seventy-seven flowers were collected from 25 areas in Korea, and 502 yeast strains were isolated from these flowers. A total of 50 species were identified by comparing large subunit rDNA gene sequence homology with the type strains of yeasts. The analysis of yeast distribution showed that the dominant yeast species were Aureobasidium pullulans, A. leucospermi, and Filobasidium magnum in each region and flower samples. Except for the above three yeast species, no species of yeasts showed any meaningful distribution among the habitat regions and sources. In conclusion, 50 species of indigenous yeasts were obtained from flowers that can be used as industrial resources, and the data could be used for further research on yeast diversity and interactions between yeast and its host.