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http://dx.doi.org/10.5010/JPB.2017.44.3.271

Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation  

Hong, Joon Ki (National Institute of Agricultural Science, Rural Development Administration)
Oh, Seon-Woo (National Institute of Agricultural Science, Rural Development Administration)
Kim, Jeong Hoe (Department of Biology, Kyungpook National University)
Lee, Seung Bum (National Institute of Agricultural Science, Rural Development Administration)
Suh, Eun Jung (National Institute of Agricultural Science, Rural Development Administration)
Lee, Yeon-Hee (National Institute of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 271-286 More about this Journal
Abstract
GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors containing two conserved QLQ and WRC domains and play critical roles in regulating the growth and development of lateral organs, such as cotyledons, leaves, and flowers. To explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), the researchers isolated seven BrGRFs (BrGRF3-1, 3-2, 5, 7, 8-1, 8-2, and 9) and constructed BrGRF-overexpressing Arabidopsis thaliana plants (BrGRF-OX). BrGRF-OX plants developed larger cotyledons, leaves, and flowers as well as longer roots than the wild type. The increase in size of these organs were due to increases in cell number, but not due to cell size. BrGRF-OX plants also had larger siliques and seeds. Furthermore, BrGRF-OX seeds produced more oil than the wild type. RT-PCR analysis revealed that BrGRFs regulated expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, the data indicates that BrGRFs act as positive regulators of plant growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed oil production.
Keywords
Cell division; GROWTH-REGULATING FACTOR; Morphogenesis; Organ size; Seed oil; Transcription factor;
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1 Cho H-T, Cosgrove DJ (2000) Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana. Proc Natl Acad Sci USA 97:9783-9788   DOI
2 Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA (2001) Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis. Plant J 26:573-582   DOI
3 Coll-Garcia D, Mazuch J, Altmann T, Mussig C (2004) EXORDIUM regulates brassinosteroid-responsive genes. FEBS Lett 563:82-86   DOI
4 Che R, Tong H, Shi B, Liu Y, Fang S, Liu D, Xiao Y, Hu B, Liu L, Wang H, Zhao M, Chu C (2016) Control of grain size and rice yield by GL2-mediated brassinosteroid responses. Nature Plants 2:15195
5 Desfeux C, Clough SJ, Bent AF (2000) Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method. Plant Physiol 123:895-904   DOI
6 Duan P, Ni S, Wang J, Zhang B, Xu R, Wang Y, Chen H, Zhu X, Li Y (2016) Regulation of OsGRF4 by OsmiR396 controls grain size and yield in rice. Nature Plants 2:15203
7 Fleet CM, Sun TP (2005) A DELLAcate balance: the role of gibberellins in plant morphogenesis. Curr Opin Plant Biol 8:77-85   DOI
8 Gonzalez N, De Bodt S, Sulpice R, Jikumaru Y, Chae E, Dhondt S, Van Daele T, De Milde L, Weigel D, Kamiya Y, Stitt M, Beemster GT, Inze D (2010) Increased leaf size: different means to an end. Plant Physiol 153:1261-1279   DOI
9 Gonzalez N, Beemster G, Inze D (2009) David and Goliath: what can the tiny weed Arabidopsis teach us to improve biomass production in crops?. Curr Opin Plant Biol 12:157-164   DOI
10 AOCS (1997) Fatty acid composition by gas chromatography. In Official Methods and Recommended Practices of the American Oil Chemists. Method Ce 1-62 Champaign IL USA
11 Kim JH, Choi D, Kende H (2003) The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis. Plant J 36:94-104   DOI
12 Horvath BM, Magyar Z, Zhang Y, Hamburger AW, BakoL, Visser RGF, Bachem CWB, Bogre L (2006) EBP1 regulates organ size through cell growth and proliferation in plants. EMBO J 25:4909-4920   DOI
13 Horiguchi G, Kim GT, Tsukaya H. (2005) The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana. Plant J 43:68-78   DOI
14 Hu J, Wang Y, Fang Y, Zeng L, Xu J, Yu H, Shi Z, Pan J, Zhang D, Kang S, Zhu L, Dong G, Guo L, Zeng D, Zhang G, Xie L, Xiong G, Li J, Qian Q (2015) A rare allele of GS2 enhances grain size and grain yield in rice. 8:1455-1465   DOI
15 Kim JH, Kende H (2004) A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis. Proc Natl Acad Sci USA 101:13374-13379   DOI
16 Kim JH, Lee BH (2006) GROWTH-REGULATING FACTOR4 of Arabidopsis thaliana is required for development of leaves, cotyledons, and shoot apical meristem. J Plant Biol 49:463-468   DOI
17 Kim JH, Tsukaya H (2015) Regulation of plant growth and development by the GROWTH-REGULATING FACTOR and GRF-INTERACTING FACTOR duo. J Exp Bot 66:6093-6107   DOI
18 Krizek BA (2009) Making bigger plants: key regulators of final organ size. Curr Opin Plant Biol 12:17-22   DOI
19 Lee BH, Ko J-H, Lee S, Lee Y, Pak J-H, Kim JH (2009) The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties. Plant Physiol 151:655-668   DOI
20 Kwon YR, Lee HJ, Kim KH, Hong S-W, Lee SJ, Lee H (2008) Ectopic expression of Expansin3 or $Expansin{\beta}1$ causes enhanced hormone and salt stress sensitivity in Arabidopsis. Biotech Lett 30:1281-1288   DOI
21 Li Y, Zheng L, Corke F, Smith C, Bevan MW (2008) Control of fi nal seed and organ size by the DA1 gene family in Arabidopsis thaliana. Genes Dev 22:1331-1336   DOI
22 Li J, Yang H, Peer WA, Richter G, Blakeslee J, Bandyopadhyay A, Titapiwantakun B, Undurraga S, Khodakovskaya M, Richards EL (2005) Arabidopsis $H^+$-PPase AVP1 regulates auxin-mediated organ development. Science 310:121-125   DOI
23 Li LC, Kang DM, Chen ZL, Qu LJ 2007. Hormonal regulation of leaf morphogenesis in Arabidopsis. J Integr Plant Biol 49:75-80   DOI
24 Li S, Gao F, Xie K, Zeng X, Cao Y, Zeng J, He Z, Ren Y, Li W, Deng Q, Wang S, Zheng A, Zhu J, Liu H, Wang L, Ping Li (2016) The OsmiR396c-OsGRF4-OsGIF1 regulatory module determines grain size and yield in rice. Plant Biotech J 14:2134-2145   DOI
25 Liang G, He H, Li Y, Wang F, Yu D (2014) Molecular mechanism of microRNA396 mediating pistil development in Arabidopsis. Plant Physiol 164:249-258   DOI
26 Liang YC, Jeon Y-A, Lim S-H, Kim JK, Lee J-Y, Kim Y-M, Lee Y-H, Ha S-H (2011) Vascular-specific activity of the Arabidopsis carotenoid cleavage dioxygenase 7 gene promoter. Plant Cell Rep 30:973-980   DOI
27 Omidbakhshfard MA, Proost S, Fujikura U, Mueller-Roeber B (2015) Growth-Regulating Factors (GRFs): a small transcription factor family with important functions in plant biology. Mol Plant 8:998-1010   DOI
28 Liu D, Song Y, Chen Z, Yu D (2009) Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis. Physiol Plantarum 136:223-236   DOI
29 Liu J, Hua W, Yang HL, Zhan GM, Li RJ, Deng LB, Wang XF, Liu GH, Wang HZ (2012) The BnGRF2 gene (GRF2-like gene from Brassica napus) enhances seed oil production through regulating cell number and plant photosynthesis. J Exp Bot 63:3727-3740   DOI
30 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plantarum 15:473-497   DOI
31 Wang Y, Henriksson E, Soderman E, Henriksson KN, Sundberg E, EngstromP (2003) The Arabidopsis homeobox gene, ATHB16, regulates leaf development and the sensitivity to photoperiod in Arabidopsis. Dev Biol 264:228-239   DOI
32 Perrot-Rechenmann C (2010) Cellular responses to auxin: division versus expansion, Cold Spring Harb Perspect Biol 2:a001446
33 Van der Knaap E, Kim JH, Kende H (2000) A novel gibberellin induced gene from rice and its potential regulatory role in stem growth. Plant Physiol 122:695-704   DOI
34 Wang F, Qiu N, Ding Q, Li J, Zhang Y, Li H, Gao J (2014) Genome-wide identification and analysis of the growth-regulating factor family in Chinese cabbage (Brassica rapa L. ssp. pekinensis). BMC Genomics 15:807   DOI
35 Ye R, Yao QH, Xu ZH, Xue HW (2004) Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development. Plant J 38:348-357   DOI
36 Zhang DF, Li B, Jia GQ, Zhang TF, Dai JR, Li JS, Wang SC (2008) Isolation and characterization of genes encoding GRF transcription factors and GIF transcriptional coactivators in maize (Zea mays L.). Plant Sci 175:809-817   DOI