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Seroprevalence of Trichinella sp. in Wild Boars (Sus scrofa) from Yanggu-gun, Gangwon-do, Korea

  • Lee, Hye-Jung;Chung, Ok-Sik;Kim, Jae-Lip;Lee, Seung-Ha;Yoo, Young-Bok;Seo, Min
    • Parasites, Hosts and Diseases
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    • v.53 no.2
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    • pp.233-236
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    • 2015
  • A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild boars had yet been performed in Korea, the present study aimed to estimate the prevalence of trichinellosis in wild boars and some species of rodents by artificial digestion and serological examinations in Yanggu-gun, Gangwon-do, the endemic area of trichinellosis. Both the wild boar and rodent muscle samples revealed no Trichinella larvae by direct examination and artificial digestion method. However, serological examinations revealed that 4 wild boar sera samples out of 118 (3.4%) were positive to Trichinella antigen. Although the recovery of Trichinella larvae ended in a failure, it is proved for the first time that the sylvatic cycle of Trichinella has been maintained in wild boars of Gangwon-do, Korea.

High Prevalence of Mycobacterium avium subsp. paratuberculosis in Wild Ducks in the Middle Area of South Korea

  • Rhim, Haerin;Cho, Yong-Il;Jang, Hye-Jin;Na, Ki-Jeong;Han, Jae-Ik
    • Journal of Veterinary Clinics
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    • v.35 no.1
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    • pp.7-9
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    • 2018
  • Mycobacterium avium subspecies paratuberculosis (MAP) causes a significant economic burden in the animal production industry. The purpose of this study was to investigate the prevalence of MAP in the feces of wild duck populations residing along a riverside close to farms in the center of Korea. From wild Spot-billed (Anas poecilorhyncha) and Mallard (Anas platyrhynchos) ducks, 128 fecal samples were collected and analyzed using multiplex real-time PCR, sequencing, and nested PCR to confirm the presence of the organism. The molecular analyses showed that 44 samples (34.4%) were positive for MAP, suggesting a high prevalence of MAP in the wild duck population. Considering the nature and habitat of wild ducks, this result suggests that the organism was introduced from contaminated water from waste of nearby farms, and that the wild ducks may act as a transmitter of the organism to other wild birds or livestock.

Antioxidative Activity and Serum Lipid Composition of Wild Grape Seed Powder in Rats fed Hypercholesterolemic Diet (고콜레스테롤혈증 유발식이를 급여한 흰쥐에서 머루씨 분말첨가가 항산화활성과 혈액지질조성에 미치는 효과)

  • Won, Hyang-Rye
    • The Korean Journal of Community Living Science
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    • v.18 no.3
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    • pp.363-368
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    • 2007
  • This study is to find out the antioxidative effect and serum lipid composition of wild grape seed powder in vivo. 20 white Sprague Dawley rats of six weeks old were divided into 2 groups and AIN-93 basic diet, high fat and cholesterol were provided. And they were examined to know how wild grape seed powder worked for antioxidative effect and serum lipid composition. For the comparing group, wild grape seed powder consisting 5% of the diet weight was provided and the quantity of protein, fat, carbohydrate, and cellulose was controlled following the analysis of the ingredients. The rats were fed for four weeks with experimental diet. Serum lipid and the antioxidant enzyme activity in blood and liver microsome were measured after 4 weeks of experiment. The results are as follows; There was no difference between the experimental groups in the initial body weight, final body weight, weight gain and FER. Food intake was higher in the group wild grape seed powder was provided than in the control group(p<0.05). Serum total cholesterol in the control group was significantly higher than that in the group wild grape seed powder was provided.(p<0.05). There was no difference serum HDL cholesterol and LDL cholesterol between the groups. Serum triglyceride showed no significant difference between the groups. In blood, glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group. The glutathione reductase activity of blood showed no difference between the groups. In liver, the glutanthione peroxidase activity was higher in the group supplemented with wild grape seed powder than in the control group(p<0.05). Glutathione reductase activity in liver showed no difference in accordance with the supplementation of wild grape seed powder.

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Optimization of Extraction Condition of Methyl Jasmonate-treated Wild Ginseng Adventitious Root Cultures using Response Surface Methodology

  • Liu, Qing;Jo, Yang Hee;Ahn, Jong Hoon;Kim, Seon Beom;Paek, Kee-Yoeup;Hwang, Bang Yeon;Park, So-Young;Lee, Mi Kyeong
    • Natural Product Sciences
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    • v.24 no.2
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    • pp.103-108
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    • 2018
  • The usage of wild ginseng (Panax ginseng C.A. Meyer) has been limited due to short supply and high price. Therefore, sufficient production as well as efficient extraction of mountain ginseng are required for the development as products. In this study, wild ginseng adventitious root cultures were prepared for efficient production with advantages of fast growth and stable production. Treatment of methyl jasmonate (MJ) to wild ginseng adventitious root cultures increased the extraction yield and antioxidative activity. Further investigation on effect of extraction conditions suggested the importance of ethanol concentration on antioxidative activity and extraction yield of MJ-treated wild ginseng adventitious root cultures. Optimized extraction condition of MJ-treated wild ginseng adventitious root cultures for maximum extraction yield and antioxidative activity was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Extraction of 1 g MJ-treated wild ginseng adventitious root culture with 30 ml of 9% ethanol at $30^{\circ}C$ produced 310.2 mg extract with 71.0% antioxidative activity at $100{\mu}g/ml$. Taken together, MJ-treated wild ginseng adventitious root culture is valuable source for wild ginseng usage and optimized extraction condition can be used for the development of functional products or folk remedies.

The Effects of Soil Surface Moisture Distribution in Perlite on Occurrence of Wild Plants (지표면의 수분분포가 야생초본류의 발생에 미치는 영향)

  • Bak, In-Young;Kim, Min-Soo
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.4 no.1
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    • pp.16-23
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    • 2001
  • This study was conducted to analyse the relation between physical characteristics of soil surface and wild plants occurrence. Lots of natural occurrence on loamy soil and a little of natural occurrence on perlite. Those were used to observe the wild plants occurrence through the duration. Natural occurrence of wild plants were observed on uniform sand, perlite, loamy soil and 2cms loamy soil layer above the perlite. Uniform sand was compared with different height of drain ditch. The results of analysis were as followed. 1. Wild plants germinated on the uniform perlite layer, they did not grow larger. Because water in large pores of perlite surface drained rapidly and evaporated easily, therefore surface remained low moisture contents. 2. A lot of weed grew on 2cms loamy layer on perlite which stratified above the perlite layer. Because perlite had plenty of soil moisture and soil moisture moved easily from perlite to loamy soil layer. 3. Uniform loamy soil had similar occurrence on the uniform perlite. It was nearly same at surface moisture distribution but lower than layered loamy soil on perlite, and the vertical distributions at soil moisture was totally lower than 2cms loamy soil layer on perlite. 4. Wild plants were grew on uniform sand on different height of drain ditch. In this case, much more wild plants were grew on which had more higher drainage ditch. The number of wild plants occurred when it was affected by soil surface moisture, drain ditch and natural occurrence of wild plants. This could be controlled by layered soil at surface moisture. Therefore weed occurrence can control in planting ground, where soil layer would not be disturbed.

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Complete 1H-NMR and 13C-NMR spectral assignment of five malonyl ginsenosides from the fresh flower buds of Panax ginseng

  • Wang, Yu-Shuai;Jin, Yin-Ping;Gao, Wei;Xiao, Sheng-Yuan;Zhang, Yu-Wei;Zheng, Pei-He;Wang, Jia;Liu, Jun-Xia;Sun, Cheng-He;Wang, Ying-Ping
    • Journal of Ginseng Research
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    • v.40 no.3
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    • pp.245-250
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    • 2016
  • Background: Ginsenosides are the major effective ingredients responsible for the pharmacological effects of ginseng. Malonyl ginsenosides are natural ginsenosides that contain a malonyl group attached to a glucose unit of the corresponding neutral ginsenosides. Methods: Medium-pressure liquid chromatography and semipreparative high-performance liquid chromatography were used to isolate purified compounds and their structures determined by extensive one-dimensional- and two-dimensional nuclear magnetic resonance (NMR) experiments. Results: A new saponin, namely malonyl-ginsenoside Re, was isolated from the fresh flower buds of Panax ginseng, along with malonyl-ginsenosides Rb1, Rb2, Rc, Rd. Some assignments for previously published $^1H$- and $^{13}C$-NMR spectra were found to be inaccurate. Conclusion: This study reports the complete NMR assignment of malonyl-ginsenoside Re, $Rb_1$, $Rb_2$, Rc, and Rd for the first time.

A Study on the Wild and Scenic Rivers System(I) -American Experience, and Suggestions for System Building in Korea- (自然景觀水系의 體系樹立을 위한 基礎硏究(I) -美國의 自然景觀水系制度 紹介 및 國內適用 事例硏究-)

  • Ahn, Tong-Mahn;Ahn, Seong-Ro;Jin, Young-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.18 no.3 s.39
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    • pp.57-70
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    • 1990
  • This study is on system building and model case study to establish a new framework of "The Wild and Scenic Rivers System" and to apply this to Korean rivers. The Wild and Scenic Rivers System of the U. S. , which had become law in 1968 for establishing wild scenic and recreational river areas, was investigated. The techniques of two American case studies about the National Wild and Scenic Rivers System designation were investigated and synthesized, and study process was adjusted to Korean river system. Additionally, pilot study was carried out by the method and results were as follows : 1) In the American Wild and Scenic Rivers System, each river was evaluated based on eligibility criteria for designation, such as remarkable scenic, recreational, fish and wildlife, cultural or other ecological values. Segmented river areas were classified into three or four grades and management plans were prepared accordingly. 2) The management of rivers in Korea has emphasized on the flood control and water resources management up to the present, and now takes a growing interest in water quality. But it has been concerned very little with wild and scenic resources conservation of river corridors. It is strongly recommended to build Wild and Scenic Rivers System in Korea for rational management and conservation of the valuable natural resource. 3) Suggested evaluation criteria for the Korean Wild and Scenic Rivers System were wildness, wildlife and plants, hydrology, scenic quality, and historical and cultural resources. The river areas may be graded into four : Positive conservation area(I), Negative conservation area(II), Negative development area(III), Positive development area(IV). Management guidelines were proposed for each grade. 4) To test the applicability of the approach, one of the major tributary of the Han-River was selected for pilot study. The result showed that the evaluation and grading system worked well. Finally, it is recommended to incorporate the Wild and Scenic Rivers System into Korean national park system, and to amend the Korean National Park Act to enable this.

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A Clinical Study on the Effects of Intravenous Wild ginseng Herbal Acupuncture on the Human Body (정맥 주입용 산삼약침이 인체에 미치는 영향에 관한 임상적 연구)

  • Kwon, Ki-Rok
    • Journal of Pharmacopuncture
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    • v.7 no.1 s.12
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    • pp.15-26
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    • 2004
  • Background : This study was conducted to evaluate the effects of wild ginseng herbal acupuncture developed for the intravenous use. Healthy male and female volunteers(n=57) went through Randomized Control Trials(RCT). Methods : For those who are under a medication due to common cold or other illnesses were excluded in the primary stage and the subjects with possible abnormalities in the pre-screening process were also excluded in the secondary stage. Then the examination groups were determined by random sampling. Experiment groups were divided into Normal saline injection group(control group), cultivated wild ginseng herbal acupuncture group(experiment group 1) and natural wild ginseng herbal acupuncture group(experiment group 2) Blood tension, body temperature, pulse, and other criteria were measured and analyzed. Results : 1. Intravenous injection of cultivated wild ginseng herbal acupuncture and natural wild ginseng herbal acupuncture didn't cause significant changes in the blood tension, pulse, body temperature, and etc. 2. No significant differences were witnessed in CBC, ESR, biochemistry of blood test and UA between the experiment groups. 3. No significant changes were noted in the thermography before and after the test in the experiment groups. 4. Some of the common physical changes occurring during and after the administration were fatigue, chest distension, and headache in all of the experiment groups. 5. Comparing general condition after one week from the termination of administration, the control group showed worst condition while as the natural wild ginseng herbal acupuncture group displayed best condition. Conclusion : From the above results, we can carefully deduce that the intravenous injection of the wild ginseng herbal acupuncture didn't show significant differences compared to injection of the normal saline. We can infer it is safe on the human body and further studies and reports must be followed.

Estimating potential range shift of some wild bees in response to climate change scenarios in northwestern regions of Iran

  • Rahimi, Ehsan;Barghjelveh, Shahindokht;Dong, Pinliang
    • Journal of Ecology and Environment
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    • v.45 no.3
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    • pp.130-142
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    • 2021
  • Background: Climate change is occurring rapidly around the world, and is predicted to have a large impact on biodiversity. Various studies have shown that climate change can alter the geographical distribution of wild bees. As climate change affects the species distribution and causes range shift, the degree of range shift and the quality of the habitats are becoming more important for securing the species diversity. In addition, those pollinator insects are contributing not only to shaping the natural ecosystem but also to increased crop production. The distributional and habitat quality changes of wild bees are of utmost importance in the climate change era. This study aims to investigate the impact of climate change on distributional and habitat quality changes of five wild bees in northwestern regions of Iran under two representative concentration pathway scenarios (RCP 4.5 and RCP 8.5). We used species distribution models to predict the potential range shift of these species in the year 2070. Result: The effects of climate change on different species are different, and the increase in temperature mainly expands the distribution ranges of wild bees, except for one species that is estimated to have a reduced potential range. Therefore, the increase in temperature would force wild bees to shift to higher latitudes. There was also significant uncertainty in the use of different models and the number of environmental layers employed in the modeling of habitat suitability. Conclusion: The increase in temperature caused the expansion of species distribution and wider areas would be available to the studied species in the future. However, not all of this possible range may include high-quality habitats, and wild bees may limit their niche to suitable habitats. On the other hand, the movement of species to higher latitudes will cause a mismatch between farms and suitable areas for wild bees, and as a result, farmers will face a shortage of pollination from wild bees. We suggest that farmers in these areas be aware of the effects of climate change on agricultural production and consider the use of managed bees in the future.

Genetic Analysis of Pigmentation Pattern in the Leopard Danio (Leopard danio 반문의 유전 양상과 생존율에 미치는 영향)

  • 이병문;강거영;송춘복
    • Journal of Aquaculture
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    • v.11 no.3
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    • pp.353-361
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    • 1998
  • Genetic analysis of body pigmentation pattern has been conducted by the reciprocal monohybrid and back crosses using the wild type zebrafish, Danio rerio, and its aquarium morphs (mutants), leopard danio. Also, the effect fo pigmentation mutation was investigated with regard to the survival rates of eggs and larvae for the first 15 days after fertilization. As a result, the pattern pigment distribution was inherited by a single gene having two alleles, and which was basically followed by the principle of dominace and segregaion in Mendelian inheritance. A locus for pigment pattern turned out to be located on an autosomal chromosome. Average survival rates estimated from the various crosses between, and within, wild type zebrafish and leopard danio were as follows ; they were 80.6${\pm}$4.8% from the crosses within leopard danios ($L{\times}L$), 70.6${\pm}$4.2% between leopard female and wild type male ($L{\times}Z$), 73.2${\pm}$2.0% between wild type female and leopard male ($Z{\times}L$), and 83.8${\pm}$6.7% within wild type zebrafish ($Z{\times}Z$). These results indicated that the leopard danio, which were reported as an "aquarium morph" several decades ago and also known as a mutant in pigmentation pattern of the wild type zebrafish, seemed to be genetically stable like the wild type of zebrafish.zebrafish.

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